MicroStation System, MicroLog Version 4.2 - DTU Systems Biology ...

MicroStation System 

MicroLog 

Version 4.2 

User Guide 

© 2007 Biolog, Inc. 

All rights reserved

Trademarks 

OmniLog is a registered trademark, MicroStation, MicroLog, MicroPlate, 

Streakerz, LongSwabs, RetroSpect are trademarks of Biolog, Inc. 

Microsoft Windows, Windows XP, Excel and Access are either registered 

trademarks of Microsoft Corporation in the United States and/or other 

countries. 

Pentium is a registered trademark of Intel Corporation. 

Macintosh is a registered trademark of Apple, Inc. 

InstallShield is a registered trademark of Macrovision Corporation. 

BDFalcon is a trademark of Becton Dickenson Co. 

Phytagel is a trademark of Sigma-Aldrich Biotechnology LP. 

Technical Service 

For technical and sales assistance, contact Biolog at: 

Address: 21124 Cabot Blvd 

Hayward, CA 94545-1130 

U.S.A. 

Tel: 510-785-2564 

(M-F, 7:30 a.m. to 5:00 p.m., PST) 

Fax: 510-782-4639 

email: tech@biolog.com 

web page: http://www.biolog.com 

MicroStation System, MicroLog Version 4.2 User Guide 

Part number: 35510 

Revisions: 2003; 2004; November 2007 

Not for human in vitro diagnostic use.

LICENSE AGREEMENT 

Notice: This is a legal agreement between you, an individual or entity (“Licensee”), and Biolog, Inc. (“Licensor”). By 

purchasing or using this software, whether it is on a diskette or preinstalled on a computer, you agree to be bound by this 

agreement. If you do not accept this agreement, please immediately return the unopened software case containing the 

complete contents, as well as any computer on which such contents were preinstalled, to Licensor or other place of purchase. 

License: Licensor grants Licensee the right to use the enclosed copy of Licensor’s MicroLog software (the “Software”) at 

the single microbiology laboratory (at one physical location) for which this license is granted (the “Site”). The Software 

shall include all related materials and documentation provided by Licensor. The Software shall be used only in the conduct 

of Licensee’s own business and Licensee shall not permit any third party to use the Software. Licensee shall make available 

implementation computer equipment and software configurations approved by Licensor as adequate for implementation of 

the Software. 

Ownership: The Software and all modifications thereto and all copies thereof are proprietary to Licensor and title thereto 

remains in Licensor. All applicable rights to patents, copyrights, know-how, trademarks, and trade secrets in the Software 

and any modifications made thereto are and shall remain in Licensor. Licensee shall not sell, transfer, publish, disclose, 

display or otherwise make available the Software or copies thereof to others. Licensee agrees to secure and protect each 

module, software product, and related documentation in a manner consistent with the maintenance of Licensor’s rights 

therein and to take appropriate actions by instruction or agreement with its employees or consultants who are permitted 

access to the Software to satisfy its obligations hereunder. Any copies made by Licensee of the Software and other programs 

developed hereunder, including translations, compilations, partial copies with modifications and updated works, are the 

property of Licensor. Violation of any provision of this paragraph shall be the basis for immediate termination of this 

Agreement. No copying or transmission of the Software is permitted. 

LIMITED WARRANTY 

For one year after the date of the invoice from Seller, Seller will either, at its sole discretion, repair or replace any product 

covered by such invoice purchased by you from Seller or Seller’s authorized distributor which, in Seller’s judgment, is 

defective in materials or workmanship, or refund the amount paid by you for such product. If the product is dated as to shelf 

life, this limited warranty shall in no event extend beyond such shelf life. In the case of third-party computer operating 

system software, this limited warranty shall extend only for thirty (30) days after the date of invoice and shall entitle you 

only to an exchange, with no right to a refund. This limited warranty does not cover: (a) computer applications software 

(which is governed by the Biolog, Inc. Software License Agreement); (b) consumable or disposable items not dated by 

Seller as to shelf life, including, without limitation, lamp assemblies, bulbs, fuses, and batteries; or (c) damage caused by 

batteries, probes or electrodes. This limited warranty does not apply to any product that has been misused, neglected, 

modified, or repaired by anyone other than an authorized service facility, or to any product requiring refrigeration or other 

special handling that is not properly refrigerated or so handled. All warranty registration cards included with the products 

must be completed and returned by you within ten (10) days after the date of purchase. Any product which is or becomes 

defective during said one year period shall be returned by you, transportation, custom duties and other fees prepaid, in the 

original carton to Seller’s principal facility in Hayward, California, or such other place which may be designated by Seller. 

You must obtain prior approval from Seller before returning any product to Seller. Transportation charges, custom duties 

and other fees for return to you shall be paid by you, and you shall bear all risk of loss or damage during transportation to 

and from Seller. Seller shall reimburse you transportation charges, custom duties and other charges incurred by you in 

returning product pursuant to this paragraph only if: (a) you have complied with all of these Terms and Conditions; and (b) 

the returned product is defective in materials or workmanship and is covered by the limited warranty provided in this 

paragraph. THERE ARE NO WARRANTIES WHICH EXTEND BEYOND THIS DESCRIPTION ON THE FACE 

HEREOF, ANY COURSE OF DEALING, CUSTOM OR USAGE OF TRADE OR COURSE OF PERFORMANCE 

NOTWITHSTANDING. THE FOREGOING IS SPECIFICALLY IN LIEU OF ALL OTHER WARRANTIES EXPRESS 

OR IMPLIED, INCLUDING THE WARRANTIES OF MERCHANTABILITY AND FITNESS FOR USE FOR A 

PARTICULAR PURPOSE AND ANY WARRANTIES THAT THE PRODUCTS DO NOT INFRINGE THE PATENT 

OR OTHER INTELLECTUAL PROPERTY RIGHTS OF THIRD PARTIES. NO AGENT OR REPRESENTATIVE OF 

SELLER HAS ANY AUTHORITY TO BIND SELLER TO ANY AFFIRMATION, REPRESENTATION OR 

WARRANT CONCERNING PRODUCTS MADE BY SELLER. IN NO EVENT SHALL SELLER BE LIABLE FOR 

LOSS OF PROFIT, LOSS OF DATA, DAMAGE TO OTHER EQUIPMENT USED IN CONJUNCTION WITH THE 

PRODUCTS WHETHER OR NOT USED PROPERLY, OR ANY OTHER INCIDENTAL, CONSEQUENTIAL, OR 

SPECIAL DAMAGES. YOU AGREE THAT SELLER’S LIABILITY FOR DAMAGES, IF ANY, SHALL NOT 

EXCEED THE PAYMENTS MADE BY YOU FOR ANY DEFECTIVE PRODUCT.

Schedule of Current Pages/Sections 

The current MicroStation System, MicroLog Version 4.2 User Guide is composed of the 

following pages or sections, dated as shown: 

Page/Section 

Date 

Cover Page 15 Apr 07 

Technical Service 1 Nov 07 

License Agreement 1 Nov 07 

Schedule of Current Pages/Sections 1 Nov 07 

Table of Contents 15 Apr 07 

Section 1 15 Apr 07 


Section 3 1 Nov 07 










Appendix Cover Page 15 Apr 07 

Appendix 1 15 Apr 07 





Appendix 6 1 Nov 07

Table of Contents 

Section Title 

1. Welcome…………………………………………………………………….. 

System Requirements……………………………………………………………. 

MicroStation/MicroLog Software Configurations………………………..……... 

MicroStation/MicroLog User Guide Access………..…………..……………….. 

21 CFR Part 11 Compliance………..…………………………………………… 

2. Installation and Registration ……..……………………………………... 

Installing the MicroStation/MicroLog Software…………………………………. 

First Log-In and Setting up the Administrator…………………………………... 

Registering your Software………………………………………………………. 

Installing Biolog Databases……………………………………………………... 

3. MicroStation System Overview…………………………………………... 

How It Works…………………………………………………………………….. 

The Identification Process………………………………………………………... 

Easy-to-Use Software…………………………………………………………….. 

Logging In and Out………………………………………………………………. 

The Math Behind the Software…………………………………………………... 

Identifying Microbes and Managing Data……………………………………….. 

4. Preparing Samples………………………………………………………….. 

Isolating a Pure Culture………………………………………………………….… 

Gram Staining……………………………………………………………………… 

Wet Prep…………………………………………………………………………… 

Characterizing Aerobic Bacteria…………………..…………………………….…. 

Characterizing Anaerobic Bacteria………………………………………………… 

Characterizing Filamentous Fungi & Yeast…..……………………………………. 

Culturing Your Microbe…………………………………………………………… 

Preparing Inocula………………………………………………………………….. 

Special Procedures for Spore-Forming Gram-Positive Rods…………………..…. 

Special Procedures for Filamentous Fungi………………………………………… 

Inoculating MicroPlates…………………………………………………………… 

Incubating MicroPlates……………………………………………………………. 

Special Procedures for Incubating Anaerobic MicroPlates……………………….. 

Sample Preparation Process for Filamentous Fungi………………………………. 

Sample Preparation Process for GN,GP,AN, YT ………...………………………. 

Sec.Page 

1.1 

1.1 

1.2 

1.3 

1.3 

2.1 

2.1 

2.2 

2.4 

2.5 

3.1 

3.1 

3.2 

3.3 

3.4 

3.5 

3.6 

4.1 

4.1 

4.2 

4.3 

4.3 

4.5 

4.5 

4.6 

4.7 

4.9 

4.13 

4.14 

4.15 

4.16 

4.17 

4.17

5. Reading MicroPlates………………………………………..………………. 

Logging In…………………………………………………………………………. 

Choosing Manual, Reader, or File Mode…………….……………………………. 

Choosing Printer Method………….……………………………………………….. 

Reading Plates Manually…………………………………………………………… 

Reading Plates Using the Plate Reader……………………………..……………… 

Reading from a Saved File………………………………………………….. 

Setting Up a Worksheet……………………………………………………………. 

Setting Up to Save Files……………………………………………………………. 

Using Worksheets to Read Multiple MicroPlates………………………….………. 

Adjusting Thresholds Manually……………………………………………………. 

Choosing a Database to Search…………………………………………….………. 

Exiting……………………………………………………………………………… 

6. Interpreting Results…………………………………………………….…… 

Reading Printouts…………………………..……………………………………… 

Assessing the Accuracy of Your ID……………………………….………………. 

Pinning Down an Uncertain ID……………………………...………………….…. 

Species Comparison………….………………………………………………….…. 

Searching and Assessing Species Information….………………………………….. 

Using the View Database Button ……………….....………………………………. 

Using Macroscopic and Microscopic Pictures (FF)..………………………………. 

Using the “Other” Row…..……….………………………………………………... 

Assessing Raw OD Values…..…..………………………………………………… 

Assessing Data Values…..…..…………………………………………………….. 

7. Data Management and Compiling Databases ..………………………… 

Backup Data File…………...………………….……………………………….….. 

Combining Data Files………..……………………….…………………………..... 

Viewing and Editing Files…..……………………………………………………... 

Compiling Data Files..……………...…………………………………………...…. 

Exporting ASCII Data………………………………………………………….…. 

Exporting to Microsoft Access……………………………………………………. 

Cluster Analysis……………………………..…………………………...…. 

8. Administration and Security…………………...……….………………… 

What is Restricted Access Mode?………………………………………………... 

Administrator Options ………………………………………………..…………. 

Creating a User List ……………………………………………………………… 

Viewing the Log-In Log …………………………………………………………. 

Interpreting the Log-In Log…………………………………………………….… 

Changing or Lost Passwords …………………………………………..………… 

Unauthorized Log-In Attempts…………………………………………………… 

9. Technical Notes…………………………………...……….………………… 

Materials List ……………………….……………………………………………... 

Media Preparation …………………………………………………………………. 

Specialized MicroPlates …………………………………………………………… 

5.1 

5.1 

5.1 

5.3 

5.3 

5.7 

5.10 

5.11 

5.14 

5.16 

5.19 

5.20 

5.22 

6.1 

6.1 

6.1 

6.2 

6.5 

6.6 

6.6 

6.12 

6.13 

6.14 

6.15 

7.1 

7.2 

7.3 

7.4 

7.7 

7.16 

7.21 

7.23 

8.1 

8.1 

8.2 

8.4 

8.5 

8.6 

8.6 

8.8 

9.1 

9.1 

9.2 

9.6

10. Frequently Asked Questions……………………………………………. 

11. Troubleshooting……………………………………………………………. 

Gram Stain Identification……………………………...………………………....… 

Culturing Microorganisms…………………………………………………….…… 

Preparing Inocula…………………………………………………………………... 

Inoculating MicroPlates……………………………………………………………. 

Incubating MicroPlates…………………………………………………………….. 

MicroStation Reader…………..…………………………………..………………. 

12. Glossary……………………………………………………………………… 

13 Appendices..……..…………..………….………………………………..… 

Appendix 1: Data Statistics for Various Plate Types……………………………… 

Appendix 2: Release 4.2 Setup Flowchart…………………………………………. 

Appendix 3: FF Setup Flowchart………………………………………………….. 

Appendix 4: Database Species List and Their Characteristics……………………... 

Appendix 5: Program Printouts…………………………………………………….. 

Appendix 6: Dangerous Pathogen Database……………………………………….. 

10.1 

11.1 

11.1 

11.3 

11.4 

11.5 

11.5 

11.6 

12.1 

13.1 

13.1 

13.2 

13.3 

13.5 

13.40 

13.52

1. Welcome 

In this section: 

�System 

Requirements 

�Installing 

MicroStation/ 


Software and 

Biolog 

Databases 

�MicroStation/ 


Software 

Configurations 

Welcome 

Welcome to Biolog's MicroStation System. The MicroStation 

System includes everything you need to use all the features of 

MicroStation software. If you ordered a MicroStation System with a 

computer, it should include the following: 

• Multimedia-compatible computer with color monitor 

• Windows ® operating system installed 

• MicroStation Reader 

• Turbidimeter 

• MicroStation/MicroLog software on CD ROM 

• Multichannel repeating pipettor 

Note: MicroLog 1 and MicroLog 2 systems do not include the 

MicroStation Reader or pipettor. 

System Requirements 

If you are installing MicroStation/MicroLog software on a computer 

system you already have, you will need the following minimum 

requirements: 

• 

• 

Pentium® PC with 512 Cache, 700 mHz or comparable 

256 MB RAM 

• 80 GB Hard Disk Space 

• CD ROM drive 

• 

• 

• 

Microsoft two-button mouse 

Monitor: 800x600 pixel resolution; 6 bit, 256 color 

Windows XP ® NOTE: 

Throughout this 

guide, references 

are made to other 

sections in the 

following manner: 

•Section 5 page 3, 

referring to the 

third page of the 

fifth section. 

, Service Pack 2 

Or, 

•Page 3.5 

referring to the 

fifth page of the 

third section. 

Note: The software is not designed to run on a Macintosh®. 

MicroStation System/MicroLog User Guide Apr 07 Section 1 � Page 1

Welcome 

Section 1 � Page 2 

Connecting the MicroStation System 

1. Unpack all materials. Your MicroStation arrives in many boxes. 

Check that all items have arrived against the packing slip. 

2. Connect the computer, monitor, keyboard, and mouse. 

3. Connect the reader, per enclosed instructions. 

4. Set up the turbidimeter, per enclosed instructions. 

5. Plug in the pipetter to fully charge the battery. 

MicroStation/MicroLog Software 


In order to make the power of the MicroStation System available to a 

variety of microbiology laboratories with different needs and budgets, 

Biolog sells three configurations of MicroStation/MicroLog software. 

This user guide contains instructions and information for all basic and 

advanced features of MicroStation/MicroLog software. Depending on 

the software configuration you purchased, some sections of the manual 

may not apply to your lab. 

Table 1.1 shows the differences between configurations. 

Software 

Specifications 

Configuration 

MicroLog 1 � Identification only 

� Visual reading only (no MicroStation Reader) 

MicroLog 2 � All advanced features, Restricted or Unrestricted Mode 

� Visual and file reading only (no MicroStation Reader) 


MicroLog 3 

� All advanced features, Restricted or Unrestricted Mode 

� MicroStation Reader (visual and file reading also possible) 

TABLE 1.1. MICROSTATION/MICROLOG SOFTWARE CONFIGURATIONS 

If you bought MicroLog1 

� Skip material dealing with the MicroStation 

Reader, Worksheets, Adjusting Thresholds, 

and Data Management, Compiling Databases 

and Restricted Access. 

If you bought MicroLog2 

� Skip material dealing with MicroStation 

Reader. 

If you bought MicroStation/ MicroLog 3 

� Read the entire User Guide. 

MicroStation System/MicroLog User Guide Apr 07

MicroStation/MicroLog User Guide Access 

Welcome 

In order to access the MicroStation/ MicroLog 4.2 User Guide, 

Acrobat Reader must be installed in your computer. If you do not have 

Acrobat Reader already installed in your computer, then a set-up 

program called “Acrobat Reader 7.0.exe exists in the Manual subdirectory 

under …\Program Files\Biolog\MLN_XX_XX (e.g. ML3 

version 4.20.05 is 42_05). Run the set-up application and follow its 

instructions. 

21 CFR Part 11 Compliance 

The MicroStation/MicroLog system is designed to exist within a 21 

CFR Part 11 environment. We provide the basic functionality to 

support our customer’s compliance efforts. 

The objective of the original regulation is to ensure integrity of 

electronic records by: 

1. Limiting Access 

2. Ensuring that original record files can not be modified. 

3. Providing documentation of what was changed, by whom 

and when. 

Within “Restricted Access Mode” the software will enforce all of the 

security Microbial identification tasks accomplished in accordance 

with the guidelines of 21 CFR Part 11, ensuring data integrity and 

security controls. Database management (audit control) will need user 

procedural controls to manually freeze files after edits are made 

The software is both password protected and encrypted. 

The MicroStation/MicroLog Software combined with the RetroSpect 

Trending and Tracking Software meets the needs of facilities that must 

adhere to strict regulatory guidelines. 


2. Installation and Registration 


�System 

Requirements 

�Installing 



Software and 

Biolog 

Databases 

�MicroStation/ 


Software 


Important! 

• The person 

installing the 

software must 

be logged in 

through the 

Windows 

operating 

system as the 

Administrator. 

Installation and Registration 

Installing the MicroStation/MicroLog Software 

1. Insert the software CD into the CD ROM drive. 

2. The software setup Installer will initialize. This will take a few 

moments to prepare. 

3. The software – InstallShield® Wizard will appear. Click Next. 

4. The License Agreement will appear. Review the terms and select 

the I accept the terms in the license agreement radio button. 

Click Next. 

5. The next screen that appears gives you the option of where to 

install the software root directory folders: 

Destination Folder - The root directory (for the software) may be 

installed anywhere on a stand alone PC or on a network drive. If 

you do install the directory on a network drive, please remember 

that this program is currently not designed for multiple users at one 

time. 

• The default/suggested installation location is 

…\Program Files\Biolog\MLN_XX_XX (e.g. ML3 

version 4.20.05 is 42_05). Click Next to accept this 

default location. 

• To choose your own location, click Change. Select the 

Look In pull down menu to browse for a location. 

Select the location and click OK. 



Note: 

The Readme 

Information is not 

printable at the 

InstallShield Wizard 

window. The file is 

located in the 

MicroStation\ 

MicroLog Software 

root directory and is 

named readme.rtf. 

Go to this location 

to open and print the 

Readme 

information. 


6. Now the InstallShield Wizard is ready to begin installation. 

Choose Back to make any changes, Cancel to exit the 

InstallShield Wizard and not install the program, or Install to 

proceed with installation. 

7. The next screen that appears asks you to enter the serial number of 

your program (found on the spine of the software jewel case). 

Enter the Serial Number and click OK. 

8. The Installing Software screen will appear while the installation 

is taking place. Click Finish when the InstallShield Wizard 

Completed screen appears. 

The following shortcut icons will now be installed on your 

desktop: 

• MLN_XX_XX.exe is for the program 

• MicroLog ID User Guide 

Please remember that the program will be accessible for up to 30 

days. After 30 days the software must be registered to use! 

First Log-In and Setting up an Administrator 

The program is ready to be opened for the first time. The program 

operates in the Restricted Access Mode. 

Restricted Access Mode requires that only the Program 

Administrator oversees and controls who has access to the program. 

The person who is designated as the Original Program Administrator 

should perform the first Log-in. This individual then manages who 

has access to the application and what tasks they can perform. If 

desired, more that one user can be assigned Administrator Privileges 

on the User List. 



The Administrator will: 

• Assign User names and passwords for those who will use the 

system. 

• Assign access privileges to each user. 

The following steps must be implemented by the user who will act as the program 

Administrator. 

1. Click on the ‘MicroStation/MicroLog’ shortcut icon on your 

desktop. 

2. The Welcome screen will appear. Click the Administrator Login 

key in the lower right hand corner. 

Note: 

Not all users will 

have full 

administrative 

privileges. To learn 

more about 

assigning user 

names and 

passwords, as well 

as setting up 

different levels of 

user access, please 

refer to Section 9. 

3. The Administrator Dialog box will open. 

• Enter a Username that is at least 1 character in length. 

• Enter a Password that is at least 6 characters in length 

and contains at least one number. The password is case 

sensitive. 

4. Click OK. 

5. When the message "Click on the Log In button at the top right to 

log in to the program" appears, click OK. 

6. Click on the Log In box located in the upper right hand corner of 

the Welcome window. A Password Dialog box will appear. 




7. Enter the Administrator username and password you set in Step 3. 

Click OK. 

8. You will now be logged in with full user privileges. 

Registering your Software 

After initial installation, the Welcome tab will show “Temporary 

Registration Days Left: 3” in red. The software will count down 

how many days you have left to register. You must click the 

Registration button to start the registration process. 

Note: 

Registration Process 

There is only 1 

registration 1. Generate a User Key and send to Biolog 

button access 

per session. 2. Load the Registration Key from Biolog 

Log-Out and 

Log-In for Follow the steps outlined below to generate a User Key: 

additional 

access. 

1. At the Welcome tab, click on the Registration button. 

2. The Registration Form window appears. 

• Each computer 

requires a separate 

registration key. 

• Your 

registration key 

should arrive 

within 48 business 

hours, providing it 

is received Monday 

to Thursday during 

regular Biolog 

business hours 

(M-F 8:30 A.M. – 

5:00 P.M. PST). 

3. Fill out every line of the Registration Form. (* Required Fields) 

4. Click the Save User Key button. 

5. A Save As window appears. Type a file name for the User Key 

File in the File Name field. The Save as Type field should show 

Text Files as the default file type. 

6. Click Save. The User Key will be saved as a text file. 

7. Click Close. 


8. E-mail the User Key File to tech@biolog.com. 


Follow the steps outlined below to load the Registration Key: 

1. Biolog will e-mail you a Registration Key. Save the attached 

registration key file on your hard drive 

(…\MLN_XX_XX\Certificates). 

2. Once you have saved your Registration key, open the 

MicroStation/MicroLog Software program, and click on the 

Registration button to open the Registration Form. 

3. Click on the Registration Key tab that is on the registration form. 

Then click the Load Registration Key button to load the 

registration key file and process the registration. 

4. The Temporary Registration box on the Welcome screen should 

now read “Registered”, and turn from red to gray. 

5. The Registration Button is no longer present. 

Installing Biolog Databases 

Installing a MicroLog database 

Install databases in any order. There are six available databases each 

on a separate CD. 



• Gram Negative (GN) 

• Gram Positive (GP) 

• Anaerobe (AN) 

• Yeast (YT) 

• Filamentous Fungi (FF)) 

• Dangerous Pathogens (DP) 

Note: Yeast or Filamentous Fungi databases requires use of a MicroStation Reader. 


1. Check to make sure you have the MicroLog database CD ROM. 

2. Put the database CD ROM into the CD ROM drive. 

3. Click Start. 

4. Select Run. Click on Browse. Click on the CD ROM drive. 

5. The field should read D:\SetUp(database).exe. If drive D is not 

your CD ROM drive, then type in the correct drive letter. 

6. Click OK. 

7. Follow the instructions on the pop-up dialogs. Make note of the 

drive and directory path into which the database is installed. 

8. To install the database into the default directory (…\Program 

Files\Biolog\MLN_XX_XX), click Finish. If you chose a 

different directory for the ML software, then type in the name of 

that same directory, before clicking Finish. 

Running MicroLog software 

Double-click the MicroLog desktop icon. 


MicroStation System/MicroLog Overview 

3. MicroStation System/MicroLog Overview 


�How It Works 

�The 

Identification 

Process 

�Easy-to-Use 

Software 

�The Math 

Behind the 

Software 

�Identifying 

Microbes and 

Managing Data 

The MicroStation System/MicroLog is an easy-to-use yet advanced tool 

for identifying and characterizing microorganisms. Our combined 

databases include over 1,900 species of aerobic bacteria, anaerobic 

bacteria, fungi and yeasts. They contain nearly all of the significant 

species encountered in diverse practices of microbiology, including 

pharmaceutical, biotechnology, cosmetic, and medical device companies; 

veterinary medicine; agriculture and environmental science; food 

processing, spoilage, and safety; reference laboratories; industrial 

microbiology; and research and education. 

MicroStation/MicroLog continues to expand and evolve to keep pace with 

progress in the field of microbiology. Every month researchers discover 

new species of microorganisms and recognize their importance. Biolog’s 

patented microbial identification technology with 95 carbon source 

utilization tests in a microtiter plate format (MicroPlate ) can recognize 

over 4 x 10 28 possible metabolic patterns. This provides room for future 

growth of the system, so that the technology will remain state-of-the-art. 

How It Works 

Biolog’s innovative, patented technology uses each microbe’s ability to 

use particular carbon sources to produce a unique pattern or “fingerprint” 

for that microbe. As a microorganism begins to use the carbon sources in 

certain wells of the MicroPlate, it respires. For bacteria, this respiration 

process reduces a tetrazolium redox dye and those wells change color to 

purple. The end result is a pattern of colored wells on the MicroPlate that 

is characteristic of that microorganism. For fungi, respiration and 

assimilation are detected. The color change in the wells is reddish orange 

due to the respiration of fungi, which reduces the dye. Assimilation or 

growth is detected by the turbidity of the well. 

A bacterial pattern is readable either visually or by a fiber optic reading 

instrument − the MicroStation Reader. This reader is required to read a 

yeast or fungal pattern. The fingerprint data is fed into the software, which 

searches its extensive database and makes a identification in seconds. By 

developing a simple tool that allows 95 simultaneous carbon source 

utilization tests, Biolog has accomplished its goal of producing an 

efficient, easy-to-use, powerful, and reliable microbe identification 

system. 

MicroStation System/MicroLog User Guide Nov 07 Section 3 � Page 1


The Identification Process 

Microbial identification involves five basic steps. These steps apply to all 

identifications. A small number of species have peculiarities that may 

require an extra step or special handling techniques. 

The Microbial Identification Process 

Step 1 

Step 2 

Step 3 

Step 4 

Step 5 

Isolate a pure culture on Biolog media 

Do a Gram stain (or wet prep for FF) 

and determine testing protocol 

Prepare inoculum at specified cell density 

Inoculate and incubate MicroPlate 

Read MicroPlate and determine ID 

Section 3 � Page 2 MicroStation System/MicroLog User Guide Nov 07

Follow 

directions 

closely to 

obtain accurate 

results. 

Step 1: Isolate a pure culture on Biolog media 


Isolating a pure culture is not always easy. For example: Bacteria often 

have sticky surfaces and cells sometimes stick together in clumps. As a 

first step to accurate microbe identification, streak agar plates using 

correct techniques to generate well isolated colonies. Always use Biologrecommended 

culture media and growth conditions. See Section 4 for full 

culturing and incubation instructions. 

Step 2: Do a Gram stain and determine testing protocol 

For bacteria, proper Gram stain technique and interpretation are the 

important second step in the ID process. See pages 4.2 and 10.1-10.2. For 

FF identification, use the wet prep test as necessary to differentiate yeasts 

from filamentous fungi. 

Step 3: Prepare inoculum at specified cell density 

Microbiologists are sometimes trained to prepare cell suspensions by 

judging cell density by eye. This practice will not yield accurate and 

reproducible results. Cell density determines oxygen concentration − a key 

parameter to control when testing microorganisms in MicroPlates. In 

addition, Biolog has carefully optimized the required inoculating fluids. 

Don’t deviate from Biolog’s inocula preparation directions See pages 4.7 - 

4.10. 

Step 4: Inoculate and incubate MicroPlate 

Pipet the specified amount of cell suspension into the MicroPlate, put the 

lid on, and incubate under the same conditions of temperature and 

atmosphere used to culture the microorganism. Biolog MicroPlates do not 

need oil overlays or color-developing chemicals. 

Step 5: Read MicroPlate and determine ID 

After an appropriate incubation time, read MicroPlates either by eye or 

using the MicroStation Reader. In either case, the pattern formed in the 

wells is entered into the software, which searches the database and 

provides an identification in seconds. 

Easy-to-Use Software 

MicroStation/MicroLog provides an easy-to-follow visual software 

interface to lead you through the identification process. The software can 



Sample 

identifiers 

List of 

ranked 

choices 

be run in a restricted access mode that provides lists of user names, 

passwords, and a hierarchy of log-in privileges. This ensures the integrity 

of electronic records by limiting access, providing documentation of 

changes made, creating audit trails, allowing you to freeze data, and 

ensuring that original file records have not been modified or deleted. 

You’ll spend most of your time using the Data window, with its areas for 

entering sample identifiers (strain name, strain number, etc.), viewing and 

entering MicroPlate reactions (positive, negative, and borderline), and 

viewing microbial identifications. 

Once you’re familiar with the system, you’ll be able to prepare your 

samples using proper techniques, read MicroPlates either singly or in 

batches, prepare worksheets to save time, build your own database, and 

analyze data using the advanced functions built into the software. 

DATA WINDOW, WITH RESULTS 

MicroPlate 

reactions 

ID box with 

first-ranked 

choice 

Logging In and Out 

First, follow these steps to Log In and Out of the program, as well as 

switch users. Remember, every person who wants to use the program 

must always enter a User ID and password to access the program. This 

provides important security to the program by putting controls on and 

keeping a record of who accesses the program. 

Logging In 

1. Click on the ‘MicroStation/MicroLog’ shortcut icon on your desktop 

2. The Welcome screen will appear, along with a Password Dialog box. 



3. Enter your Username and Password. Click OK. 

4. You will now be Logged In, and the Welcome interface will appear. 

Timed Log Out 

The software program has a default setting to automatically Log Out if the 

program has not been used for 15 minutes. The Login Time Out screen 

will appear. Simply select the Click Here button to stop the Log Out 

process and continue using the program. Otherwise the program will log 

off, return to the Welcome window, and display a new Log In dialog box. 

Logging Out and Switching Users 

There are 3 ways to end your use of the program: 

1. Select the Exit Tab and then click the Exit button. 

2. Click on the Change Users button in the center of the Welcome 

window. The Password Dialog box will be displayed for the new user 

to enter their username and password. 

3. Click on the windows page “X” button. 

The Math Behind the Software 

MicroStation/MicroLog software uses extensive computer algorithms to 

take the information from the observed pattern and compare it to the 

database. 

In simple terms, the software rapidly compares the positive-negativeborderline 

color pattern in the MicroPlate to the species pattern in the 

appropriate database. The patterns that most closely match your microbe’s 

pattern are shown on the screen in ranked order. Before making a decision 

on the result, the software considers the possibility that even the firstranked 

choice may not be a good match. It looks to see whether the first 

choice match is really “close enough” to be acceptable. If not, a “No ID” 

designation will result. 

For GN, GP, and AN databases, MicroStation/MicroLog uses a newly 

developed pattern matching method called Progressive ID (PID). This 

method more accurately identifies species patterns by considering the 

progressive sequence in which purple wells are formed. Typically, 

microorganisms will use their favorite carbon sources most rapidly and 

completely, resulting in dark purple wells that form quickly. Lesspreferred 

carbon sources are consumed slowly or incompletely, resulting 

in slower-forming or lighter purple color. The extra information 

considered by the PID matching method brings a higher level of 

consistency and accuracy, representing another innovation in Biolog’s 

technology. 



For YT and FF databases, MicroStation/MicroLog uses the developed 

pattern matching method called Endpoint ID (EID). This method more 

accurately identifies species patterns by considering the daily endpoint 

determinations. For the YT MicroPlate, turbidity may be caused by either 

growth or color formation. For the FF MicroPlate, both growth (measured 

by turbidity) and color formation are measured independently for each 

well. 

Identifying Microbes and Managing Data 

The software is structured to move you easily through identifying 

microbes and managing data. Navigation tools include a menu bar at the 

top of the window, menu tabs to move from window to window, dropdown 

lists to choose from pre-set choices, selection bars to reach detailed 

setup windows, and fields to type in specific data. 

Menu bar 

Menu tabs 

Selection 

button 

SET UP WINDOW (SHOWING NAVIGATION TOOLS) 

The software will guide you in answering the following questions: 

• Do I plan to read MicroPlates visually? 

• Will I use the reader or will I read back from a file? 

• Do I want an automatic printout of results? 

• Do I want to select a specific printer? 

Drop-down 

lists 



If you plan to enter MicroPlate reactions manually you’ll enter sample 

identifiers using the mouse, keyboard, or on-screen methods to indicate 

positive, negative, or borderline MicroPlate reactions. 

ENTERING SAMPLE IDENTIFIERS AND MICROPLATE REACTIONS 

In addition, the software allows you to: 

• Use worksheets 

• Save data 

• Edit files and build databases 

• Adjust thresholds 

• View a database 

• Perform cluster analysis 



Using worksheets 

Worksheets allow you to enter all of your sample identifiers ahead of time. 

This speeds the reading process and enables you to read high MicroPlate 

volumes more efficiently. 

WORKSHEET (WITH SAMPLE ENTRIES) FOR BATCHING MICROPLATES 

Saving data 

You can save your results into files and manage them by using the Output 

options on the Set Up window. 

FIELDS FOR NAMING AND SAVING FILES 

File output 

area 



Editing files and building databases 

You can also select which database you want to use – Biolog’s or your 

own. Advanced Data Management functions allow you to view data by 

strain or plate type, edit data files, build your database, and perform a 

number of other database management techniques. 

DATABASE MANAGEMENT WINDOW (FOR ADVANCED DATABASE FUNCTIONS) 

Adjusting thresholds 

Another software advanced feature for bacteria is the capability of 

adjusting thresholds by using histograms. This function may be 

appropriate if you feel that the automatic reading of your MicroPlate does 

not match your visual interpretation. 

HISTOGRAM AND MICROPLATE DATA (FOR MANUAL ADJUSTMENT OF THRESHOLDS) 



Viewing databases 

The View Database function allows you to access end-point and/or 

progressive data of specific organisms in each of Biolog’s databases. 

The function also allows you to view the Macroscopic and Microscopic 

image libraries included in the Filamentous Fungi (FF) Database. 

EXAMPLE OF MICRO PICTURES FROM THE FF DATABASE IMAGE LIBRARY 

Performing cluster analysis 

Cluster analysis is an advanced feature that offers visual ways to simplify 

interpretation of data sets. You’ll be able to view dendrograms, twodimensional, 

and three-dimensional cluster diagrams. 

1-D 

dendrogram 



2-D 

dendrogram 

3-D 

dendrogram 


4. Preparing Samples 


�Isolating a Pure 

Culture 

�Gram Staining 

�Wet Prep 

�Characterizing 

Aerobic Bacteria 


Anaerobic Bacteria 


Filamentous Fungi 

and Yeast 

�Culturing your 

Microbe 

�Preparing Inocula 

�Special Procedures 

for Spore-Forming 

Gram-Positive Rods 


for Filamentous Fungi 

�Inoculating 

MicroPlates 

�Incubating 

MicroPlates 


for Incubating 

Anaerobic MicroPlates 

�Sample Preparation 

Process 

Biolog special 

agar: 

BUG = Biolog 

Universal Growth 

BUA = Biolog 

Universal 

Anaerobe 

BUY = Biolog 

Universal Yeast 

2% ME = 2% Malt 

Extract Agar 

Preparing Samples 

As with any system, the precision and accuracy of MicroStation/ 

MicroLog results require proper sample preparation. The most common 

identification problems result from improper lab technique and using nonrecommended 

media. Using good sterile technique and the correct media 

greatly increase the likelihood of problem-free microbe identification. 

If you’re thinking of using non-recommended media – don’t. The 

extensive MicroLog databases were specifically developed using Biolog’s 

carefully selected media. Using non-specified media causes changes in the 

physiology of microbes. Luxuriant growth and full metabolic activity are 

required for the system to work well. 

The flowchart on page 2.2 provides an outline of the identification 

process. The tables on page 4.16-17 give a comprehensive overview of the 

sample preparation process, as does the flowchart in the Appendices. See 

pages 9.2-9.6 for detailed media preparation instructions. Refer to 

Appendix 6 when using the DP (Dangerous Pathogen) database. 

For additional information refer to Instructions for Use specific to the 

plate type (supplied with each purchase of MicroPlates). 

Isolating a Pure Culture 

Culture your sample on Biolog’s general-purpose culture medium. Nearly 

all the bacterial species in the MicroLog databases will grow on Biolog 

Universal Growth media (BUG or BUA), generally with the addition of 

either 5% sheep blood or maltose. Prepare media according to package 

insert. Yeast are cultured on BUY (YT), while filamentous fungi (FF) are 

cultured on 2% Malt Extract Agar. . 

Each shipment of Biolog MicroPlates comes with instructions. Refer to 

these for detailed directions about how to grow a healthy culture. In 

general: 

• Make certain you have a pure culture (a single strain). 

• Incubate at optimal temperature. Most bacteria should be grown at 

35-37° C or 30° C depending on genera. (A few gram positive, 

anaerobic organisms, yeasts and filamentous fungi are grown at 

26° C.) 



Preparing an 

inoculum 

directly from 

a mixedgrowth 

plate 

will cause 

identification 

problems. 

Use the 

colony 

magnifier 

lamp to 

closely 

examine 

colonies. 

• Do not allow cultures to grow for too long. Maximum growth is 24 

hours for most bacteria, 72 hours for yeasts, and 10 days for most 

fungi. Some exceptionally slow-growing or fastidious bacteria may 

require 48 hours of growth or the use of multiple growth plates. 

Checking to see if your culture is pure 

Colonies on the plate that seem to be isolated, may in fact be the result of 

mixed growth. This is especially true with Staphylococcus species. 

Careful visual examination is essential to ensuring that a culture is pure. It 

may be useful to use a colony magnifier lamp to assist in the examination. 

If a colony shows any hint of pleomorphism, it is probably not a pure 

culture and requires additional re-streaking and isolation. 

Carefully examine areas of confluent growth. If the lawn is not uniform in 

texture and color, this may indicate that the culture is not pure. Once 

again, restreak for isolation. 

Note: Unless your organism is a very slow grower, we don’t recommend 

using lawn growth. 

The opposite problem can also occur; sometimes a culture may be pure, 

but give the appearance of heterogeneity. This is due to a rather common 

phenomenon whereby microorganisms produce more than one colony 

type. To be certain of its identity, purify and test each colony type 

individually. Be aware that different colony types of the same strain may 

ID but give different phenotype profiles. 

Gram Staining 

A Gram stain is essential in order to select the proper media and 

MicroPlates for testing bacteria. Once your initial culture has been 

incubated sufficiently, perform a standard Gram stain. Determine the 

following: 

• Is the microbe a bacterium or a yeast? 

• Is the microbe gram positive or negative? 

• Are the cells cocci or rods? 

• Do the cells form spores? 

Based on the source of the sample, the initial growth conditions, and the 

Gram stain, you should know what basic type of microbe you have. See 

pages 10.1-10.2 for specific Gram stain information. Refer to a basic 

microbiology textbook if you are unsure of these procedures. 

Section 4 � Page 2 MicroStation System/MicroLog User Guide Apr 07

Attention! 

Please refer to 

Appendix 6 when 

using the DP 

(Dangerous 

Pathogen) 

Database. 

Enteric: gramnegative 

bacteria 

belonging to 

Enterobacteriaceae 

group 

Non-enteric: 

gram-negative 

bacteria not 

belonging to 

Enterobacteriaceae 

group 


Wet Prep 

A wet prep is performed to determine if the microbe is a yeast or 

filamentous fungi. This is an optional test, required only if the form is 

uncertain. Refer to a basic microbiology textbook if you are unsure of this 

procedure. 

Characterizing Aerobic Bacteria 

Gram-negative and gram-positive aerobes require further investigation to 

determine proper handling and identification. 

Characterizing gram-negative microbes 

If your microbe is gram negative, several additional tests will help 

determine whether your microbe is non-enteric (GN-NENT), enteric (GN- 

ENT) or fastidious (GN-FAS) as well as determine the proper setup 

protocol. This has important implications in regards to choice of 

incubation temperature, inoculum density, and inoculating fluid. 

Performing an oxidase test 

• Do an oxidase test on all gram-negative organisms. Refer to a basic 

microbiology textbook if you are unsure of this procedure. 

• If the oxidase test is positive, you have a non-enteric (GN-NENT) 

microbe. 

• If the oxidase test is negative you most likely have an enteric (GN- 

ENT) microbe. 

• If you are uncertain about whether the microbe is enteric or nonenteric, 

set up a TSI slant. 

Setting up a TSI slant 

• Prepare a TSI slant on oxidase-negative microbes. Refer to a basic 

microbiology textbook if you are unsure of this procedure. 

• If the TSI slant shows a K/K (alkaline/alkaline) or K/A w 

(alkaline/weak acid) reaction, your microbe is non-enteric (GN- 

NENT). 

• If the TSI slant shows an A/A (acid/acid) or K/A (alkaline/acid) 

reaction, your microbe is enteric (GN-ENT). This is true with a few 

exceptions (such as Pasteurella species=A/A, or Acinetobacter 

A/late A), which can be weak oxidase positive. 



Recognizing fastidious gram negatives 

• Fastidious gram-negative bacteria are primarily isolated from the 

respiratory tracts of mammals. 

• These bacteria grow poorly or not at all on BUG + B medium. Some 

Moraxella species do grow well on BUG + B, but should be set up as 

fastidious gram negatives. They generally stain as gram-negative 

diplococci. 

• They grow much better on chocolate agar at 35-37° C in an 

atmosphere of 6.5% CO2. 

Characterizing gram-positive microbes 

If your microbe is gram positive, pay special attention to the cell 

morphology in the Gram stain. Note the following: 

• Determine whether the microbe is a coccus or a rod. 

• If you are unsure if your organism is a coccus or a rod (e.g., coccobacillus), 

set up a MicroPlate and select the GP-COCCUS-ROD 

strain type option to determine the correct identification. 

• If the microbe is a rod (GP-ROD), determine whether it is sporeforming 

bacillus (GP-ROD SB, consisting of Bacillus and species 

formerly called Bacillus). Spore-formers require special handling and 

testing. See pages 4.10- 4.12. 

• It can be difficult to recognize Bacillus species from a Gram stain, but 

most can be recognized by colony morphology. They are often fast 

growers, forming colonies that have unusual characteristic textures 

(e.g., slimy, crusty, dry, embedded, or forming skin-like pellicles). 

Prepare a wet mount to look for spores, retractile ovals or spheres. 

Performing a catalase test 

It is advisable to perform a catalase test (3% hydrogen peroxide) on GP- 

COCCUS and GP-ROD isolates. The catalase reaction can help you verify 

or narrow down the species identification. Refer to a basic microbiology 

textbook if you are unsure of this procedure. 

If the organism is catalase-negative, this generally indicates a slow grower 

(CO2 may be required). Use a swab or loop to subculture the organism 

onto the agar to obtain a lawn of growth. You may need more than one 

agar plate. 

Do not perform a catalase test from a blood plate, it may give false 

positive results. 



Characterizing Anaerobic Bacteria 

Gram-negative anaerobic rods require further investigation to determine 

proper handling and identification. 

Characterizing rapidly-growing, gram-negative microbes 

If your microbe is rod-shaped, anaerobic, gram negative, and grows 

rapidly, a kanamycin disk test will help differentiate Fusobacterium from 

Bacteroides/Prevotella. This has important implications in regards to 

choosing the appropriate inoculation protocol. 

Performing a kanamycin disk test 

• Streak rapidly-growing, gram-negative rods on BUA plates. 

• Add a 1 mg kanamycin disk to the first quadrant (immediately after 

streaking, before incubating the organism). 

• Incubate the plate overnight. 

• If the bacterium is sensitive (a 10 mm zone of clearing will surround 

the kanamycin disk), you have a presumptive Fusobacterium. 

• If the bacterium is resistant (no or


Media shorthand: 

B = blood 

M = maltose 

T = thioglycolate 

ME = malt extract 

Culturing Your Microbe 

From a single isolated colony subculture your microbe onto the proper 

agar medium. Most of the species in Biolog’s database are relatively fast 

growers. However, if your microbe grows slowly, you may need to streak 

(subculture) more than one agar plate. 

Incubate according to instructions in package insert. In general: 

• Incubate most organisms for 16-24 hours. Very slow-growing 

species and yeasts may require 48 hours. Slow-growing anaerobic 

bacteria may require even longer incubation. Incubate filamentous 

fungi for 5-10 days (sporulation of isolate). Slower growing 

filamentous fungi may require longer incubation for sporulation. 

• Don’t over-incubate your culture. Excess incubation can cause 

microbes to enter a stationary phase, during which they lose 

viability and metabolic activity. 

• For best results, subculture anaerobic bacteria twice on BUA. 

Prepare the suspension from the second plate only. 

• For filamentous fungi, subculture less hyphae for fast growing 

fungi and more hyphae for slow growing fungi. 

Table 4-1 and Appendix 2 will help you select the correct culture medium. 

Organism Type Organism 

Abbreviations 

Gram Negative Non-Enteric** GN-NENT BUG + B 

Gram Negative Enteric GN-ENT BUG + B 

Gram Negative Fastidious GN-FAS Chocolate 

Gram Positive Cocci 

Gram Positive Rods (non-spore 

forming) 

Gram Positive Rods (spore forming 

bacillus) 

GP-COCCUS 

GP-ROD 

BUG + B 

Culture Media 

GP-ROD SB BUG + M + T 

Anaerobes AN BUA + B 

Yeasts YT BUY 

Filamentous Fungi (and select yeast 

species) 

FF 2% ME 

TABLE 4-1: SELECTING THE CORRECT CULTURE MEDIUM 

**Many agriculture organisms should be grown on BUG (w/o Blood). For 

listing, see Appendix 4. 


STOP! 

Is your 

suspension 

density 

accurate? Did 

you calibrate 

using Biolog’s 

Turbidity 

Standards? 

Preparing Inocula 


Once your microbe is isolated and cultured, prepare a liquid inoculum. Refer 

to instructions enclosed with Biolog MicroPlates for detailed directions. In 

general: 

• The inoculum MUST be within the range specified by the turbidity 

standards accompanying your database (+ 2% T). 

• The inoculum MUST be uniformly suspended. If a bacterial organism 

forms clumps, you will need to use special techniques to achieve a 

homogenous suspension. (see “Dry Tube Method” page 4.11) 

Table 4-2 will help you decide which suspension medium to use: 

Organism Type Inoculating Fluid Turbidity Standards Inoculum Density 

Gram Negative Non-Enteric GN/GP-IF GN-NENT 52% T 

Gram Negative Enteric GN/GP-IF + T GN-ENT 61% T 

Gram Negative Fastidious GN/GP-IF + T GP-COC & GP-ROD 

& GN-FAS 


Gram Positive Rods (non-spore 

forming) 

Gram Positive Rods (spore forming 

bacillus) 

GN/GP-IF + T GP-COC & GP-ROD 

& GN-FAS 

20% T 

20% T 

GN/GP-IF GP-ROD SB 28% T 

Anaerobes AN-IF AN 65% T 

Yeasts Sterile water YT 47% T 

Filamentous Fungi (and select yeast 

species) 

FF-IF FF 75% T 

TABLE 4-2: SELECTING THE CORRECT INOCULATING FLUID, TURBIDITY STANDARDS, AND CELL DENSITY 

Adding thioglycolate to inoculating fluid 

Some microbes (see Table 4-2) require the addition of thioglycolate (T) to 

the inoculum. Thioglycolate acts as an anticapsule agent; it decreases 

production of bacterial capsules so that strains give more consistent 

patterns. Biolog provides you with droppers of thioglycolate. To add 

thioglycolate: 



Don’t forget 

to add thioglycolate 

when working with: 

GN Enteric 

GN Fastidious 

GP Cocci & Rods 

Remember, 

your microbes 

are alive. 

Treat them 

with care. Use 

fresh cultures. 

Caution! 

Use special care! 

Do not 

excessively 

vortex FF-IF 

suspensions. 

The FF-IF is 

viscous. 

It will trap air 

bubbles that will 

interfere with 

absorbance 

readings. 

1. Hold reagent dropper upright and point tip away from you. Using a 

dissecting hemostat fully crush ampule close to it’s center one time 

only. Tap the bottom on benchtop a few times. 

Caution: Do not manipulate dropper any further, as the plastic may 

puncture, causing injury. 

2. Invert the dropper for convenient drop-by-drop dispensing of reagent. 

3. Dispense precisely 3 drops of concentrated thioglycolate into your 

inoculating fluid. Do not use more than 3 drops per 18-20 ml. This 

gives you a 5 mM final concentration. 

4. Each dropper contains about 15 drops. You can continue to use an 

open dropper for the rest of the day, and then discard the remainder. 

Adding sodium salicylate to inoculating fluid 

If gram-positive bacteria are false positive even after you’ve added 

thioglycolate to the inoculating fluid, try adding sodium salicylate: 

1. Add thioglycolate as described above. 

2. Add 1 ml of sterile 100 mM sodium salicylate to 18-20 ml of GN/GP- 

IF. 

3. Proceed with sample preparation. 

Preparing inocula 

Once you’ve added thioglycolate (if necessary), prepare the inocula: 

1. Establish the appropriate turbidity range on your turbidimeter by 

adding and subtracting 2% T to the percent transmittance measured 

with the appropriate turbidity standard. 

2. Dip a sterile swab into the inoculating fluid to moisten it. 

3. Lift cells from the agar by rolling the swab over the colonies, rather 

than sliding across them. Be sure not to pick up any agar. 

4. Twirl the swab against the inside surface of the tube (above the fluid 

line) to gently dissolve colonies. 



5. Dip the swab into the fluid and stir with a up-down motion to the 

bottom of the tube to create a uniform suspension. You can also use a 

sterile transfer pipette to mix without creating an aerosol. Recap the 

tube and invert if using Biolog’s GN/GP-IF. 

6. Adjust the inoculum density so it is within the specified turbidity 

range. You can change the density by adding more cells (to increase 

density) or more inoculating fluid (to lower density). Be sure to vortex 

or mix gently before taking the final reading. 

7. Examine your suspension and make certain that it is homogeneously 

suspended and free of clumps. It is essential that the cell suspension 

does not contain clumps. If there are only a few clumps, allow them to 

settle to the bottom, pouring off the supernatant. If the bacterial 

suspension is not homogenous, use the special dry tube procedure 

described in Step 4 on page 4.11 and in Section 10. 

8. For anaerobic bacteria, prepare the suspension in batches of no more 

than six at a time. The time from beginning the first suspension to 

finishing the last suspension should not exceed 5 minutes. 

Special Procedures for Spore-Forming Gram- 

Positive Rods 

This section describes the procedure and details the techniques found to 

provide consistent identification of spore-forming gram-positive rods 

(Bacillus species). Bacillus species require a special agar media and 

streaking technique to keep cells in a vegetative growth phase and 

minimize spore formation. Most species are strong producers of capsular 

polysaccharide. This polysaccharide material interferes with cell testing 

and causes cells to clump, making it difficult to prepare cell suspensions. 

Thioglycolate is used in this special procedure to reduce polysaccharide 

production. Preparing a uniform cell suspension free from clumps is 

important for accurate identification. 

Always use the following special swabbing and streaking technique with 

Bacillus species: 

1. Isolate a pure culture of the Spore-Forming Gram-Positive Rod. 

2. Subculture the spore-forming gram-positive rod on BUG plus Maltose 

plus Thioglycolate (BUG + M + T). This will stimulate cells into 

vegetative growth phase and decrease cell clumping. Prepare the agar 

media according to the following procedure. 

a. Add 8 drops of thioglycolate (Biolog P/N 73011) from the dropper 

ampoule into 3 mL of sterile water. Mix thoroughly. 



b. Use a sterile swab (Biolog P/N 3023 or 3021) wet with the 

thioglycolate solution to spread a thin film across the entire surface 

of the BUG + M agar plate (Biolog P/N 71103). 

c. Allow the thioglycolate to dry on the agar before streaking the sporeforming 

gram-positive rod strain (takes about 5 minutes). 

3. With a Biolog sterile Streakerz stick (Biolog P/N 3025 or 3026), touch 

an isolated colony and transfer to the BUG + M + T agar media. Make a 

plus sign (+) on the center of the agar media. See Figure 1. 

Figure 1: Plus Format to Streak 

A 

a. If the organism is a fast grower and spreads, make a single very 

thin plus sign (+). This will avoid overgrowth and sporulation. 

b. If the organism is a very slow grower and does not spread then 

make up to three (3) streaks in the shape of the letter N in each 

direction of the plus. Leave a small distance between the three 

streaks to promote vegetative growth on all three streaks. Prepare 

multiple agar plates (2 to 3) to insure that enough cells are 

available to prepare the inoculation suspension. 

PLUS “+” STREAKING METHOD CELL GROWTH AFTER INCUBATION 

Section 4 � Page 10 MicroStation System/MicroLog User Guide Apr 07 

B 

c. The goal of this streaking technique is to limit cell growth to one 

(1) to three (3) lines so that the cells along the edges have a good 

supply of nutrients. This keeps the cells in an active state and 

decreases sporulation. 

4. Incubate at 30°C for 16-20 hours; do not exceed 16 hours for fast-growing 

strains.


5. Prepare a cell suspension with the following “Dry Tube Method” for 

mucoid or dry colony types. 

a. Use a sterile, dry glass test tube. 

b. Using a Streakerz stick, take cells starting at the ends and edges of 

the “Plus” sign as shown by the arrows in the Figure 1. The cells 

located outside the boxed area illustrated in Figure 1 are the most 

metabolically active cells. Avoid picking cells from the boxed area 

that are more likely to have sporulated. Be careful not to scrape up 

agar from the plate while harvesting the cells. 

c. Some mucoid strains as well as strains that become embedded in 

the agar may not adhere to the Streakerz stick making it difficult to 

transfer cell mass to the dry test tube. In this case, use the edge of a 

sterile microscope slide to scrape across the inoculation lines on 

the agar plate and recover cell mass. Be careful not to dig in to the 

agar. Transfer the cell mass from the slide to the inner wall of a dry 

test tube with the aid of a Streakerz stick. 

d. Crush and break up the cell mass against the side of the tube. Use 

an up and down motion in combination with a circular motion to 

crush and spread the cells against the inner wall of the dry glass 

tube. Continue until a sufficient mass of cells adheres to the wall of 

the glass tube forming a uniform thin film on the inside surface 

free of clumps. 

e. Transfer approximately 5 mL from a tube of GN/GP-IF (Biolog 

P/N 72101) into the glass tube containing the cells. Use an up and 

down rubbing motion to slide the bacterial film from the tube wall 

downward, into the GN/GP-IF. Continue until all cells are 

suspended in the inoculation fluid. 

f. Examine the cell suspension. Hold the tube up to the light and see 

if the cells are completely suspended or if clumps and strings 

remain. If clumps or strings remain, follow one these methods to 

remove them: 

Method 1: If the suspension is a mixture of single cells 

and clumps, let the mixture stand at room temperature until 

the clumps and debris have settled to the bottom. Use the 

homogenous cell suspension layer free of debris. If the 

suspension contains only strings and clumps (observed with 

highly mucoid strains like Bacillus amyloliquefaciens and 

licheniformis), place the suspension test tube in a warm (36 ± 

1°C) incubator or water bath for up to 20 minutes. The heat 



aids in liquefying the cell mucous. Swirl the contents of the 

tube occasionally during the incubation. Look for the 

disappearance of the distinct mucoid strings as evidence that 

the sample is ready. 

Settling of the debris is critical to eliminating clumps 

and obtaining a homogeneous cell suspension for 

accurate identification. This step may take up to 20 

minutes. 


OR 

Method 2: Filter the cell mixture through a 70 µm cell 

strainer (BD Falcon 352350) to pull out the cell debris 

from the homogeneous cell suspension. 

g. Prepare a homogeneous cell suspension. Place a new tube of 

GN/GP-IF into the Biolog Turbidimeter. Adjust the zero of the 

turbidimeter for the tube. Carefully remove the homogenous cell 

suspension using a transfer pipette (Biolog P/N 3019) making sure 

that the cell debris in the bottom of the tube is not taken. Add the 

cell suspension drop wise, with mixing to the tube of GN/GP-IF in 

the turbidimeter. Use a swab to stir and mix the cell suspension 

into the GN/GP IF. Continue to add cells to achieve a cell turbidity 

of 28% ± 2%. Uniformly suspended cells typically give stable 

turbidimeter readings whereas remaining clumps and strings cause 

the turbidimeter to fluctuate as they pass across the light path. 

Remaining clumps can be removed by letting the tube stand until 

the clumps settle. Remove the uniformly suspended cells to 

inoculate the MicroPlate. 

h. If more cells are needed to get the required cell density, repeat the 

dry tube method preparation using a new empty, sterile dry glass 

tube. Do not use the same tube that is wet with GN/GP-IF. 

6. Inoculate a GP2 MicroPlate following the procedure in the Instructions 

for Use. 

7. Incubate at 30°C for 4 – 6 hours and/or 16 – 24 hours to allow the pattern 

to form.

General hints for handling Bacillus 

� 

� 

� 

� 

� 


Always grow Bacillus species on BUG + M + T for identification. 

Keep the incubation time on BUG + M + T as short as possible to 

reduce sporulation (i.e., incubate for 16 rather than 24 hours if the 

organism grows fast). 

For slow growing strains you may need to use multiple (2 to 3) BUG + 

M + T agar plates to obtain enough cell mass. 

Use the dry tube method described above to properly prepare the 

inoculum. Allow the suspension to stand until all clumps settle to the 

bottom of the tube. Be patient. 

Bacillus cereus and Bacillus thuringiensis are considered 

indistinguishable by biochemical assays. These two species are listed in 

the Biolog database as Bacillus cereus/thuringiensis. Bacillus anthracis 

is also very closely related to these taxa. 

Special Procedures for Filamentous Fungi 

Because of their potentially pathogenic nature, filamentous fungi need 

special care and handling. 

Laboratory Safety 1 

Sporulating cultures require extra handling precautions to prevent spores 

or conidia from escaping into the air. Manipulating sporulating cultures 

can sometimes present a risk of infection or allergy, contaminate 

laboratory air, and contaminate bacterial cultures. We recommend that 

filamentous fungi be handled in a biological safety cabinet. This practice 

also diminishes the risk of infection by dimorphic fungal pathogens. 

Follow the guidelines in this chapter for all specimen preparation, 

characterization, inoculum preparation, and MicroPlate inoculation. 

General hints for handling filamentous fungi 

1. Incubate subcultured filamentous fungi in a closed container. Place the 

agar plates in a plastic container or in sealed plastic bags on trays. 

1 Several organizations have published guidelines for laboratory safety in microbiology − the Centers for Disease Control and 

National Institutes of Health (1988), the Medical Research Council of Canada (1990), and the World Health Organization (1993). 



Caution! 

Pipet the 

inoculum into a 

MicroPlate 

within 

20 minutes! 

Use sterile 

technique. 

2. Do NOT add a source of moisture to this container. Mycelial growth 

will overwhelm the agar plate and cause crossover contamination of 

other cultures. 

3. Incubate the 2% ME agar at 26° C for 5-10 days to induce conidia 

formation (incubate longer if required). Incubate yeasts for 1-2 days. 

Inoculating MicroPlates 

Inoculate the suspension into one of the following Biolog MicroPlates: 

• GN2: For all gram-negative aerobic bacteria 

Caution! 

Accurately 

pipet 

recommended 

volumes. 

• GP2: For all gram-positive aerobic bacteria 

• AN: For all anaerobic bacteria 

• YT: For all yeasts visceral 

• FF: For all filamentous fungi and select yeast strains 

Pipet the inoculum into a Biolog MicroPlate within 20 minutes. Waiting 

any longer may cause inaccurate identification. Anaerobic bacteria are 

especially sensitive to delays. If you are running a batch of MicroPlates, 

set them up (from preparing the inoculum to pipetting into the MicroPlate) 

so you will not exceed the 20 minute deadline. 

Inoculating protocol 

1. Label the MicroPlate with the organism name/number and plate type 

(e.g., GN, GP). Label the side of the MicroPlate itself, not the lid. 

2. Using aseptic technique, pour the cell suspension into a multichannel 

pipette reservoir. 

3. Firmly attach eight sterile tips to the pipettor. 

4. Fill the tips with the suspension. Check to see that all tips fill equally. 

5. Prime the tips by dispensing once back into the reservoir. The 

electronic pipettor will do this automatically. 

6. Fill all MicroPlate wells by placing the tips at an angle inside the top 

of the wells . Take care not to splash from one well to another. Avoid 

contamination. Avoid touching the bottom of the wells, which could 

transfer carbon sources. 

• Gram negatives and gram positives � 150 µl per well 

• Anaerobes, yeasts and filamentous fungi � 100 µl per well 


MicroPlates 

of aerobic 

bacteria can 

be read after 

4-6 hours of 

incubation. If 

reactions are 

insufficient, 

read again at 

16-24 hours. 


7. If the fluid level in the tips gets low, refill and continue dispensing 

until all wells are full. 

8. Cover the MicroPlate with its lid. 

Incubating MicroPlates 

As soon as you dispense the suspension, incubate the MicroPlate using the 

appropriate temperature, atmosphere, and time conditions. Table 4-3 (page 

4-15) and Appendix 2 will help you select the correct conditions. 

• For aerobes: Use a moist chamber during incubation to prevent 

evaporation. A plastic container with wet paper towels on the bottom 

should be sufficient. 

• For filamentous fungi: MicroPlates must be incubated in a closed 

container or in sealed plastic bags on trays. Do not add a source of 

moisture. Mycelial growth will overwhelm the MicroPlate and cause 

reaction crossover within the MicroPlate. 

Organism Type Temperature Atmosphere Incubation Time 

Gram Negative Non-Enteric 30° C Air 4-6 and 16-24 

hours 

Gram Negative Enteric 35-37° C Air 4-6 and 16-24 

hours 

Gram Negative Fastidious 35-37° C 6.5% CO2 4-6 and 16-24 

hours 


Gram Positive Rods (nonspore 

forming) 

Gram Positive Rods (spore 

forming bacillus) 

35-37° C or 

30° C or 26° C (if 

required) 

30° C or 

55° C (if required) 

Anaerobes 35° C or 

30° C or 26° C (if 

required) 

Air or 

6.5% CO2 (if 

required) 

4-6 and 16-24 

hours 

Air 4-6 and 16-24 

hours 

Anaerobic-H2 

free 

20-24 hours 

Yeasts 26° C Air 24, 48, or 72 

hours 

Filamentous Fungi (and 

select yeast species) 

26° C Air 24, 48, 72 or 96 

hours 

TABLE 4-3: SELECTING CORRECT INCUBATING CONDITIONS 



Special Procedures for Incubating Anaerobic 

MicroPlates 

Caution! 

If you’re 

working with 

anaerobes: 

�Use 

anaerobically 

prepared media. 

�Inoculate 

MicroPlates in 

air. 

�After 

inoculation, wait 

10 minutes 

before moving 

the MicroPlates 

into a hydrogenfree 

anaerobic 

jar. 

1. In most cases, leave AN MicroPlates in their foil pouch until 

immediately before inoculation. The only exception is if you presume 

your organism is either Bacteroides or Prevotella. If your gramnegative 

rod is resistant to kanamycin (see page 4.5) and has grown 

well enough overnight to prepare a suspension, open the AN 

MicroPlate foil pouch and expose it to air for 20 minutes before 

inoculation. 

2. For all species, keep the air exposure time consistent. Do NOT exceed 

5 minutes between the time you begin and end inoculating AN 

MicroPlates for a single run. You should be able to inoculate six 

MicroPlates in 5 minutes. If the run requires more than six MicroPlates, 

use two people or divide the number of suspensions into smaller 

batches. 

3. Wait 10 minutes after inoculating the AN MicroPlate before placing it 

in an anaerobic jar. This is necessary to slightly oxidize the buffer. 

• Rectangular jars can hold six or 24 MicroPlates, are easy to work 

with, and are inexpensive 

• 3.5 liter jars hold up to nine MicroPlates 

• 10.5 liter jars hold up to 28 MicroPlates and can stand upright 

4. Use an anaerobic, atmosphere-generating system that does not produce 

hydrogen. Organisms with strong hydrogenases will reduce the 

tetrazolium in all wells if hydrogen is present. Add an anaerobic 

indicator strip to monitor the atmosphere. Call Biolog Technical 

Service for suggestions on where to find anaerobic supplies. 

5. Incubate most anaerobes at 35° C for 20-24 hours. 

6. After incubation, check the color of the MicroPlates before you open 

the anaerobic jar. MicroPlates should be clear with purple wells. Once 

you expose them to air, negative wells will slowly take on a faint 

green-blue color. If this green-blue color is present before you open the 

jar, the atmosphere was probably not fully anaerobic. 


Sample Preparation Process 

For Filamentous Fungi 

Initial Culture Medium 2% ME 2% ME 

Wet Prep Results Hyphal elements Yeast cells 

Microbe Type Filamentous Fungi Yeast 

Culture Medium 2% ME 2% ME 

Temperature 26° C 26° C 

Atmosphere Air Air 

Culture Incubation Time 5 - 10 days 2 days 

Inoculating Fluid FF-IF FF-IF 

Inoculum Turbidity/ 

75% T 

75% T 

Turbidity Standard 

FF 

FF 

MicroPlate Type/µl per well FF 

FF 

100 

100 

Incubation time (hours) 24, 48, 72, 96 24, 48, 72, 96 

ABBREVIATIONS 

2% ME = 2% Malt Extract Agar 

FF = Filamentous Fungi 

%T = percent transmittance 

IF = inoculating fluid 




Initial culture 

medium 

Gram stain 

results 

Characterization 

test 

Characterization 

test 

Sample Preparation Process For Gram Negatives, Gram Positives, Anaerobes and Yeasts 

BUG + B* BUA + B BUY 

Gram stain and observe cell morphology 

Oxidase positive 

requires 30° C 

� Gram Negatives � � Gram Positives � 

Anaerobes 

� 

Kanamycin test for 

rapidly-growing, gramnegative 

rods 

Oxidase negative and 

TSI=K/K or K/A w 

Oxidase 

negative and 

TSI=A/A or 

K/A 

Requires CO2 or chocolate agar 

or < 1 mm colonies 

on BUG + B 

Microbe type GN-NENT GN-ENT GN-FAS GP-COCCUS-ROD, 

GP-COCCUS, 

GP-ROD 

Culture medium BUG + B or 

TSA + B 

BUG +B or 

TSA + B 

Temperature Typically 30º C Typically 35- 

37º C 

GP-ROD SB (spore-forming 

bacillus) 

Chocolate BUG + B BUG + M + T (8drops in 

3ml H2O) “+”streaking 

Atmosphere Air Air 6.5% CO 2 Air or 6.5% CO 2 

(if required) 

Inoculating 

fluid 

Inoculum 

turbidity/ 

Turbidity 

standard 

GN/GP-IF 

(if A1 well is pos, add 

thioglycolate) 

52% T 

GN-NENT 

GN/GP-IF 

+ T (3 drops) 

61% T 

GN-ENT 


Yeasts 

� 

AN YT 

BUA + B BUY 

Typically 35-37º C Typically 35-37º C Typically 30º C Typically 35-37° C 26° C 

GN/GP-IF 

+ T (3 drops) 

20% T 

GP-COC & GP- 

ROD & GN-FAS 

GN/GP-IF 

+ T (3 drops) 

(if A1 well is pos, 

add 1 ml salicylate) 

20% T 

GP-COC & GP-ROD 

& GN-FAS 

Air ANA-H 2 free Air 

GN/GP-IF 

(NO THIO) 

28% T 

GP-ROD SB 

AN-IF Water 

MicroPlate GN2 

GN2 GN2 

GP2 

GP2 

AN 

YT 

type/ 

µl per well 

150 

150 

150 

150 

150 

100 

100 

Incubation time 

(hours) 

4-6, 16-24 4-6, 16-24 4-6, 16-24 4-6, 16-24 4-6, 16-24 20-24 24, 48, 72 

*Note: Agricultural bacteria may be grown on BUG without blood.and at 30° C for enterics. 

ABBREVIATIONS 

TSI = Triple Sugar Iron Slant; A = Acid, A W = Weak Acid, K = Alkaline; BUG + B = Biolog Universal Growth Medium + blood; BUG + M = Biolog Universal Growth Medium + maltose; T = thioglycolate; 

%T = percent transmittance; GN-NENT = gram-negative non-enteric; GN-ENT = gram-negative enteric; GN-FAS = gram-negative fastidious; GP-COCCUS & GP-COC = gram-positive cocci; GP-ROD = 

gram-positive rod; GP-ROD SB = gram-positive rod, spore-forming bacillus; AN = anaerobe; YT = yeast; IF = inoculating fluid 

65% T 

AN 

47% T 

YT

5. Reading MicroPlates 


� Logging-In 

�Choosing Manual, 

Reader or File Mode 

� Reading Plates 

Manually 

or using the Plate Reader 

�Reading from a 

Saved File 

� Setting Up a Worksheet 

�Setting Up to Save Files 

�Saving Files 

�Using a Worksheet to 

Read Multiple MicroPlates 

�Adjusting Thresholds 

Manually 

�Choosing a Database to 

Search 

�Exiting 

Reading MicroPlates 

Depending on which MicroStation/ MicroLog software version you 

are using, the process of reading MicroPlates can be done in a 

simplified way or using the detailed method of creating worksheets. 

• MicroLog 1 software offers only the simplified reading process 

(entering MicroPlate reactions manually’ “by eye”, printing the 

results, and interpreting the results. 

• MicroLog 2 software uses the same manual reading process as the 

MicroLog 1 but also allows you to save results, as well as create a 

user built database. 

• MicroStation/ MicroLog 3 software allows you to create 

worksheets to read multiple MicroPlates, save files, work with 

databases, and use advanced functions (such as cluster analysis and 

threshold adjustment) 

Logging-In 

Unless the software has been set up to run in Unrestricted mode, the 

MicroStation system/ MicroLog software will always require user login 

before beginning the process of entering information and reading 

MicroPlates. 

Follow the instructions for logging-in on page 3.2. 

Choosing Manual, Reader or File Mode 

You can run the identification process in three modes: 

• Manual mode allows you to enter MicroPlate reactions by hand 

SEE Section 5, Page 3 

• Reader mode is used in conjunction with the MicroStation reader, 

where MicroPlate reactions are read and entered automatically. 

SEE Section 5, Page 7 

• File mode lets you recall and view MicroPlate data already stored 

in a file. SEE Section 5, Page 10 

Note: While running in Restricted mode, you have access to these 

features only if you have “Log-In” and “Set Up” privileges. 



Set Up tab 

Select read 

mode here 

Select 

reader here 

Worksheet 

drop-down list 

To select whether you want to work in manual, reader, or file mode, 

click the Set Up tab on the Welcome window. 

WELCOME WINDOW 

Selecting Data Input (Read) mode 

1. Use the Data Input Mode drop-down list to select Manual, 

Reader, or File. 

SET UP WINDOW (WITH MANUAL MODE SELECTED) 

2. Select the correct MicroStation reader. Molecular Devices or 

Bio-Tek. 

Printer 

selections 

3. Select Yes or No in the Use Worksheet drop-down list. For further 

information on using a worksheet, see Section 5, page 12-16. 


Write Data 

to Data 

File dropdown 

list

Caution! 

Entering 

reactions 

manually is 

NOT 

recommended 

for 

YT and FF 

MicroPlates. 


4. Select Yes or No in the Write Data to Data File drop-down list. 

For further information on writing to a data file, see Section 5 

pages 15-16. Note: If the program is operating in Restricted 

Access Mode, this option defaults to Yes, and only users with SET 

UP privileges have the ability to change this option. 

Choosing printer method 

1. Click the Automatic Printout drop-down list on the Set Up 

window to select Yes (if you want to automatically print out your 

result) or No (if you don’t want automatic printout). 

2. Click the Print in Text Mode drop-down list to select Yes (if you 

want speedier printing) or No (if you want higher resolution 

printing). 

3. Click the Printer Setup bar if you need to select a printer. This bar 

is not necessary for text printing and will disappear if you select 

text mode. 

Reading Plates Manually 

1. Use the Data Input Mode drop down menu to select Manual as the 

Data Input Mode. 

2. Click the Data tab on the Menu Bar. The Data window appears. 

DATA WINDOW (IN MANUAL MODE) 



When you’re entering 

sample identifiers, 

make sure you select 

the correct strain type. 

The default entry in 

that field is “NOT 

SELECTED.” If 

default strain type is 

selected, no database 

will be searched 

Entering sample identifiers 

1. Current Time: Your computer’s clock automatically tracks time. 

2. Plate Number: Type in the MicroPlate number. 

3. Sample Number: Type in the sample ID number. 

SAMPLE IDENTIFIER SECTION OF DATA WINDOW 

4. Plate Type: Use the drop-down list to select the type of 

MicroPlate you used. 

5. Strain Type: Use the drop-down list to select the strain type you 

are working with. 

• For an initial bacterial query use one of the following: GN-NENT, 

GN-ENT, GN-FAS, GP-COCCUS, GP-ROD, GP-ROD SB, AN- 

ALL (use the NON-APPLICABLE default for other MicroPlate 

types.) 

• Secondarily, use GN-ALL or GP-ALL to screen organisms of 

questionable type and morphology as required. 

• If an ID is obtained using GN-ALL or GP-ALL be sure to check 

that the correct protocol for that ID was used. 

• For FF query Use the FF-AIR or FF-FOOD database for FF 

MicroPlates, depending on the source of the organism to be 

identified. These databases also contain the most commonly 

isolated species from the other databases (Yeast, Aspergillus, 

Colletotrichum, Fusarium, Penicillium, Trichoderma). The other 


Remember: 

Light purple 

reactions are 

considered 

positive as 

long as the 

color is 

noticeable 

when 

compared to 

the A1 

reference 

well. 


databases are intended for use by researchers working on specific 

genera, not for routine food and air isolates. 

6. Strain Name, Number: If you have additional information, enter 

it in these optional fields. 

7. Incubation Time: Use the drop-down list to select the hours that 

the MicroPlate has incubated. 

Note: A 240-hour incubation time is listed for the FF database, 

however, there is no database for 240 hours. That listing is a research 

tool. 

8. Other: If you have additional information, enter it in this optional 

field (colony morphology, culture conditions, etc.). An optional 

comment can be entered in this field. 

Entering reactions manually 

Entering reactions in manual mode requires visual assessment of 

MicroPlate reactions. 

Table 5-1 will guide your visual assessment: 

Color Density How To Assess It 

Same as A1 well (usually colorless) Negative 

Noticeable purple color (more than A1 well) Positive 

Extremely faint color, Not sure Borderline 

TABLE 5-1. VISUALLY ASSESSING WELL COLOR DENSITY 

1. From the Data window, click Read Next (directly under sample 

information). The Manual Entry of Data window appears. 

MANUAL ENTRY OF DATA WINDOW (TO ENTER MICROPLATE REACTIONS) 



2. Enter reactions into the on-screen MicroPlate, using any of the 

methods shown below. 

3. Click OK to read the Plate 

4. The Data screen appears and displays the read result. 

SELECT YOUR PREFERRED WAY TO MANUALLY ENTER REACTIONS 

Mouse Keypad or Keyboard Method 

Use your mouse to maneuver around the on-screen keypad or use the computer keyboard to enter reactions on 

the Manual Entry of Data window. 

“1” key � negative 

“2” key � borderline 

“3” key � positive 

up and down arrows � use to navigate around the wells 

“home” key � returns cursor to A1 well 

“end” key � moves cursor to H12 well 

Mouse Cursor Method 

Select the radio button you want, then click your cursor in desired wells. With this method, you can also click 

and drag across areas to change large blocks of wells. 

● Positive � makes selected well(s) positive 

● Borderline � makes selected well(s) borderline 

● Negative � makes selected well(s) negative 

All or Nothing Method 

One click to make the whole MicroPlate positive or negative, then use radio buttons and your cursor to make 

alterations. 

■ – All Neg � makes all wells negative 

■ + All Pos � makes all wells positive 


Caution! 

The Detailed 

Reader Setup 

window contains 

troubleshooting 

tools. Don’t 

change anything 

without 

consulting 

Biolog Technical 

Service. 

Initialize Reader bar 

Reading Plates Using the Plate Reader 


1. Use the Data Input Mode drop-down list to select Reader (to use 

the reader). 

2. Select the correct MicroStation reader. Molecular Devices or 

Bio-Tek. 

3. The Initialize Reader and Detailed Reader Setup selection bars 

appear. Use the Com Port drop-down list to assign 

communications (com) port 1 thru 5. 

SET UP WINDOW (WITH READER MODE SELECTED) 

Select Com port here 

4. Click the Initialize Reader selection bar. The Reader Ready field 

changes from No (red) to Yes (green). 

Note: If the Reader Ready field does not change to Yes (green) after 1 

minute, make sure the reader is connected properly and turned on. See 

Section 11 if you have trouble initializing the reader. 

5. Select Yes or No in the Use Worksheet drop-down list. For 

information on using a Worksheet see Section 5, Page 11-14. 

6. Select Yes or No in the Write Data to Data File drop-down list. 

For information on Saving Files see Section 5, page 15-16. 

Entering Reactions Using the Reader 

* For FF strain type query see section 4 page 5. 

1. Make sure the reader is initialized (on the Set Up window). 

2. Enter sample identifiers. 




-Current Time: Your computer’s clock automatically tracks 

time. 

-Plate Number: Type in the MicroPlate number. 

-Sample Number: Type in the sample ID number. 

SAMPLE IDENTIFIER SECTION OF DATA WINDOW 

-Plate Type: Use the drop-down list to select the type of 


-Strain Type: Use the drop-down list to select the strain type 

you are working with. 

a. For an initial bacterial query use one of the following: 

GN-NENT, GN-ENT, GN-FAS, GP-COCCUS, GP- 

ROD, GP-ROD SB, AN-ALL (use the NON- 

APPLICABLE default for other MicroPlate types.) 

b. Secondarily, use GN-ALL or GP-ALL to screen 

organisms of questionable type and morphology as 

required. 

c. If an ID is obtained using GN-ALL or GP-ALL be sure 

to check that the correct protocol for that ID was used. 

d. For FF query Use the FF-AIR or FF-FOOD database 

for FF MicroPlates, depending on the source of the 

organism to be identified. These databases also contain 

the most commonly isolated species from the other 

databases (Yeast, Aspergillus, Colletotrichum, 


Check your 

MicroPlate 

before 

loading. 

Make sure 

the reaction 

pattern is 

well 

developed. 


Fusarium, Penicillium, Trichoderma). The other 

databases are intended for use by researchers working 

on specific genera, not for routine food and air isolates. 

-Strain Name, Number: If you have additional information, 

enter it in these optional fields. 

-Incubation Time: Use the drop-down list to select the hours 

that the MicroPlate has incubated. 

Note: A 240-hour incubation time is listed for the FF 

database, however, there is no database for 240 hours. 

That listing is a research tool. 

-Other: If you have additional information, enter it in this 

optional field (colony morphology, culture conditions, etc.). 

-Comment: An optional comment can be entered in this field. 

3. Wipe the bottom of the MicroPlate to remove condensation and 

fingerprints. 

4. Place the MicroPlate on the reader tray with the A1 well at the top 

left-hand corner. 

5. Gently push the MicroPlate down until it snaps into a level position 

in the reader tray. 

6. Remove the MicroPlate lid. 

Note: For FF, leave the lid on. Replace it with a new lid if 

condensation is present. 

7. Click Read Next on the Data window. 

8. The reader calibrates itself, pulls the MicroPlate inside, takes a 

reading, displays the result on the Data window, and ejects the 

MicroPlate. Briefly do a visual check of the reaction with the +/b/- 

called by the auto-thresholding function (see pg 5.20 if adjustment 

is required). 



Select file name here 

Reading from a Saved File 

Arrow 

indicates 

selected 

entry 

1. Use the Data Input Mode drop-down list to select File (to view 

reactions from a saved file) 

2. The Input Data File Name field appears. Click in the empty field 

and move through the listed folders to find the file you want to 

read and click on it. This will bring the file name up on the Set up 

screen. 

SET UP WINDOW (WITH FILE MODE SELECTED) 

3. In the Write Data to Data File drop-down list 

a. Select No. 

4. Click the Data tab. 

5. Click Select Read 

6. A list of saved data appears. Select the MicroPlate you want to 

read and click OK. 

LIST OF SAVED DATA 


What’s a 

Worksheet? 

A worksheet is a 

spreadsheet 

within 



software. Use it 

to log in and 

read multiple 

MicroPlates 

efficiently. 

7. The Data page will show the record. 

a. Click Print to print the record. 

b. Click Read Next to view the next record. 

c. Click Select Read to select another record. 

8. Repeat steps 6 thru 7 to view all records. 

Setting Up a Worksheet 


Worksheets offer an efficient way to log in and read multiple 

MicroPlates. By entering multiple sample identifiers and MicroPlate 

reactions, you simply read MicroPlates sequentially as the worksheet 

indicates, without stopping to enter more information. If you’re 

working with over five MicroPlates, using a worksheet is faster. If 

you’re reading ten MicroPlates or more, worksheets will save 

significant time. 

Worksheets also allow you to: 

• Have one shift set up a worksheet, and the second shift read the 

MicroPlates 

• Avoid entering data fields more than once if you plan to read plates 

at multiple incubation time points 

Printing worksheets gives a record of batch activity. You can use a 

worksheet whether in manual or reader mode. Worksheet use is 

optional. 

Note: If the program is running in Restricted mode, you have 

access to this feature only if you have “Log-In” privileges. 

1. On the Set Up window, select Yes on the Use Worksheet dropdown 

list. 

2. Click on the Worksheet Name field. A Worksheet File dialog 

box appears. Type the desired file name (W4C extension) or 

choose a previously created worksheet to edit. (Note: Save 

worksheets that have been created in the “DataFile” folder, or 

create a separate Worksheet folder. This will assist you in 

recalling the files at a later date.) 



Naming 

Worksheets: 

Don’t use back- 

or forward 

slashes in file 

names. 

Worksheet Entry 

Selection Bar 

Moving Around 

a Worksheet: 

CLICK � Click 

in a row to select 

a sample. (Look 

for the small 

arrow next to the 

selected sample 

number.) 

Click to add an entry 

NAMING OR RETRIEVING A WORKSHEET (IN MANUAL AND READER MODES) 

3. Click the Worksheet Entry selection bar. The Worksheet 

window appears. 

BLANK WORKSHEET (TO ENTER SAMPLE IDENTIFIERS AND REACTIONS) 


4. Click Add Entry. The Plate Information window appears. 


PLATE INFORMATION WINDOW (TO ENTER DATA INTO A WORKSHEET) 

5. For each MicroPlate, enter the following information: 

• Plate Number: Default begins with 1 (changeable) 


• Sample Number: Type in the sample ID number (optional 

field). 

• Plate Type: Use the drop-down list to select the type of 


• Strain Type: 

o For an initial bacterial query use one of the following: 

GN-NENT, GN-ENT, GN-FAS, GP-COCCUS, GP- 

ROD, GP-ROD SB, AN-ALL (use the NON- 

APPLICABLE default for other MicroPlate types.) 

o Secondarily, use GN-ALL or GP-ALL to screen 

organisms of questionable type and morphology as 

required. 

o If an ID is obtained using GN-ALL or GP-ALL be sure 

to check that the correct protocol for that ID was used. 

o Use the FF-AIR or FF-FOOD database for FF 

MicroPlates, depending on the source of the organism 

to be identified. These databases also contain the most 

commonly isolated species from the other databases 

(Yeast, Aspergillus, Colletotrichum, Fusarium, 

Penicillium, Trichoderma). The other databases are 

intended for use by researchers working on specific 

genera, not for routine food and air isolates. The YT 

MicroPlates does not have a list of strain types from 

which to choose. 



This arrow shows 

which sample you’ve 

selected to read. 

• Strain Name, Number, Other: Enter other information in these 

optional fields. (In the “Other” field, you can enter colony 

morphology, culture conditions, etc.). 

6. After each entry, click the selection bars as desired: 

• Save, Clear − to save data, to advance MicroPlate numbers 

incrementally, to clear all fields for entering information on the 

next MicroPlate 

• Save, Keep − to save data, to advance MicroPlate numbers 

incrementally, to keep current entries in all fields 

• Save, Done − to return to the worksheet 

7. When you’re finished setting up the worksheet, click OK. 

Editing a worksheet 

1. To edit a worksheet, right click on the row you want to edit. The 

Plate Information window reappears. Edit fields as desired. 

WORKSHEET (WITH SAMPLE ENTRIES) 

2. Sort entries (Plate Number) by clicking Sort Worksheet. 

3. To delete an entry click on the row and then click Delete Record. 

Setting Up to Save Files 

Note: If you are planning to compile your own database, see Section 7 

before saving files. 


If you plan to 

compile your 

own database, 

see Section 7 

before saving 

files. 


1. Click the Write Data To Data File drop-down list on the Data 

window to select Yes (if you want the software to save files) or No 

(if you don’t want to save files). 

Note: This feature gives a default answer of Yes in the Write Data To 

Data File and the Auto Write to Data File fields when the program is 

running in Restricted mode. “Set Up” privileges are required to 

change these defaults. 

2. If you selected No, the two fields below will close. If you selected 

Yes, two new fields remain. Click on the Output Data File Name 

field. A Save As dialog box appears. Enter the desired file name 

(D4C extension) or choose a previously created data file. 

3. Use the Auto Write To Data File drop-down list to select Yes (if 

you want to automatically save your results) or No (if you don’t 

want to automatically save your results). 

Note: For easier 

editing and 

compiling of saved 

data in the future, 

separate different 

plate types into 

individual data files. 

CLOSE-UP OF OUTPUT PORTION OF SET UP WINDOW 

Note: If you are working in Restricted mode, new entries are saved as 

Read Only files. Original files cannot be edited. Make a backup for 

editing. 



Using Worksheets to Read Multiple MicroPlates 

To efficiently read a batch of MicroPlates, use a worksheet. Make sure 

you’re in the desired mode (reader or manual) and that you’ve selected 

Yes for worksheet use on the Set Up window. Select the desired file 

name. 

Reading multiple MicroPlates in manual mode 

Incubation time list 

Use Select Read to 

open the worksheet 

1. Select Manual mode on the Set Up window. 

2. Click the Data tab on the menu bar. The Data window appears. 

DATA WINDOW 

3. Use the Incubation Time drop-down list to select the desired 

incubation time. 

4. To read from an open Worksheet: 

• Click Select Read to go to the desired worksheet. 

• Click on the sample number you want to read (an arrow will 

appear next to the sample number you select). 

• Click OK to read the MicroPlate. The Manual Entry of Data 


• If necessary, click Cancel to return to the worksheet. 

5. To read MicroPlates sequentially: 

Use Read Next to 

read worksheet 

entries sequentially 


Remember: 

Light purple 

reactions 

are 

considered 

positive as 

long as the 

color is 

noticeable 

when 

compared to 

the A1 

reference 

well. 


• Click Read Next to sequentially read the next MicroPlate on 

the worksheet without returning to the Worksheet window. 

• A Confirmation window appears. Click OK if it displays the 

MicroPlate you wish to read. 

• The Manual Entry of Data window appears. 

• If necessary, click Cancel to return to the Data window. 

Assessing reactions 

Entering reactions in manual mode requires visual assessment of 

MicroPlate reactions. SEE Section 5, page 5 for more information on 

reading reactions manually. 

Table 5-1 will guide your visual assessment: 

Color Density How To Assess It 

Same as A1 well (usually colorless) Negative 

Noticeable purple color (more than A1 well) Positive 

Extremely faint color. Not sure Borderline 

Entering reactions 

TABLE 5-1. VISUALLY ASSESSING WELL COLOR DENSITY 

1. Enter reactions into the on-screen MicroPlate using any of the 

methods shown on page 5.6. 

2. Click OK. 

MANUAL ENTRY OF DATA WINDOW (TO ENTER PLATE REACTIONS) 

3. Click Print to print report (if you did not select Automatic 

Printout). 



4. Click Save to save data (if you did not select Auto Write to Data 

file.) 

5. Repeat Steps 1-4 for all MicroPlates. 

Reading multiple MicroPlates in reader mode 

1. Select Reader on the Set Up window. SEE Section 5, page 7 for 

further information on using the Reader. 

SET UP WINDOW (TO SELECT READER MODE AND INITIALIZE READER) 

2. Click Initialize Reader to set up the reader. Make sure that red No 

changes to a green Yes. 

3. Click the Data tab on the menu bar. The Data window appears. 

4. Use the Incubation Time drop-down list to select the number of 

hours the MicroPlate has incubated. 

5. Place the correct MicroPlate in the reader. 

6. To work from an open worksheet: 

• Click Select Read to go to the selected worksheet. 

• Click on the sample number you want to read. An arrow will 

appear next to the sample you select. 

• Click OK. The reader calibrates itself, pulls the MicroPlate 

inside, takes a reading, displays the result on the Data 

window, and ejects the MicroPlate. 

• If necessary, click Cancel to return to the worksheet. 

7. To read plates sequentially: 


Caution! 

Manually adjusting 

thresholds will 

change SIM and 

DIST values. These 

changes override 

automatic 

thresholds. This is 

an advanced 

technique. Call 

Biolog Technical 

Service if you need 

help. 


• Click Read Next to sequentially read the next MicroPlate on 

the worksheet without returning to the Worksheet window. 

• A Confirmation window appears. Click OK if it displays the 

MicroPlate you want to read. The reader calibrates itself, 

pulls the MicroPlate inside, takes a reading, displays the 

result on the Data window, and ejects the MicroPlate. 

• If necessary, click Cancel to return to the Data window. 

8. Click Print to print the report (if you did not select Automatic 

Printout). 

9. Click Save to save data (if you did not select Auto Write to Data 

file.) A Confirmation window appears. 

10. Repeat Steps 5-9 for all MicroPlates. 

Adjusting Thresholds Manually 

The software allows you to view histograms of MicroPlate patterns. In 

most cases, use of this feature should not be necessary to obtain 

accurate results. However, this advanced technique can be useful if 

you feel that the automatic reading of your MicroPlate does not match 

your visual interpretation. You can adjust either or both of the 

thresholds to override the automatic reading and obtain what you feel 

is the correct result. You can only adjust thresholds in Reader mode 

and for only GN, GP and AN plates 

1. Click on the small DW Data tab at the upper right edge of your 

on-screen Microplate (on the Data window). You’ll see an onscreen 

MicroPlate of your sample, showing optical densities. 

2. Click the DW Histogram tab. A histogram of the current ID 

selection appears. 

3. The histogram is divided into three lateral sections, which 

represent color distribution within the MicroPlate. Wells with low 

and high color density are on the left and right sides of the 

histogram. The middle section (between the two vertical lines) 

represents wells with borderline reactions. 



Wells with high and low 

color density 

Wells with borderline 

color density 

HISTOGRAM (ON LEFT) AND OPTICAL DENSITIES (ON RIGHT) 

4. Click the Manual radio button to make adjustments. 

5. Use the horizontal scroll bars under the histogram to make 

adjustments to the thresholds. The top scroll bar adjusts the 

negative threshold (white line); the bottom scroll bar adjusts the 

positive threshold (red line). Green lines represent the automatic 

threshold levels. 

6. Continue until you feel the adjusted threshold corresponds to your 

visual read of the MicroPlate. 

7. Click Apply button to update thresholds. 

Note: FF MicroPlate types will have Pos/Neg, Data, and Histogram tabs for both 

color and turbidity. 

Choosing a Database to Search 

The software allows you to select which database you want to search. 

If you have created User (custom) databases you can search the 

MicroLog databases, the User database or both. For information on 

creating a User database, see Section 7. MicroLog databases may not 

be selected as a User database. 


Click here to make 

adjustments

Wells with high and low 

color density 

Wells with borderline 

color density 

You’ll see a Data 

Management 

Menu bar on the 

Database 

window. See 

Section 7 for 

data 

management 

instructions. 

HISTOGRAM (ON LEFT) 


1. Click the Database tab on the Set Up window. The Database 


DATABASE WINDOW (TO SELECT DATABASE TO SEARCH) 

2. Use the drop-down list to select the database you want. If you 

select a User or MicroLog/User database, the User Progressive 

Database and User End-Point Database (YT & FF) fields appear. 

Click on the appropriate field and a Database File dialog box 

appears. 

3. Select the desired database by choosing the file name. For End- 

Point databases, the file name will include the hour (e.g., for a 24 

hour read, select “demo24.eid”). 

4. Click OK. 

Click here to 

make 

adjustments 

(manual) 

Note: When you’re conducting a MicroLog/User database search, a “U” 

will appear on the Species ID ranked list on the Data window to denote 

species in the User database. Also, you will see a ML/user designation in the 

upper left corner of the organism choices. 



Exiting 

When you’re finished, click the Exit tab on the menu bar. Click Click 

Here to Exit. 

Note: If you change your mind after clicking the Exit menu tab, you 

can go back to your work by clicking any tab on the menu bar. 


6. Interpreting Results 


� Reading 

Printouts 

�Assessing the 

Accuracy of your 

ID 

�Pinning Down 

an Uncertain ID 

�Species 

Comparison 

�Searching and 

Assessing Species 

Information 

� Using 

Macroscopic and 

Microscopic 

Pictures 

�Using the 

Other Row 

� Accessing 

Raw OD values 

Interpreting Results 

Check the bottom section of the Data window. The software identifies 

your microbe in the form of a ranked list. Entry #1 is the best match 

selected from the database. The other identifications are ranked in 

descending order of pattern match. Scroll down to see the whole list. 

CLOSE-UP OF RESULTS SECTION OF DATA WINDOW 

The ID box 

Final identification results are displayed in the ID box at the top of the 

results area of the Data window. The software considers the 

possibility that even the #1 ranked species may not be a good enough 

match. In this case, you will see “No ID” in the ID box. 

Reading Printouts 

Every time you read a MicroPlate, the printer will issue a hard copy 

report. The information on these reports varies, depending on whether 

you are working in manual or reader mode. See Appendices for plate 

data statistics (A-1), and sample printouts and explanation of entries 

(A-5). 

Assessing the Accuracy of Your ID 

If the answer to the following three questions is yes, you can feel 

confident that ID #1 is accurate. 

• Are the top-ranked ID choices on the list all the same (or closely 

related) genera? 

• Check the SIM (similarity index value) rating of ID #1. Is it greater 

that .5? 

• Check the DIST (distance) rating of ID #1. Is it greater than two 

distance points away from the 2 nd choice. 



Understanding 

the language of 

microbe 

identification: 

% PROB – 

allows you to 

compare our IDs 

to other systems 

that use this type 

of calculation 

SIM – tells you 

how good each 

match is 

DIST – tells you 

the number of 

mismatches 

between your 

MicroPlate 

results and the 

database pattern 

for that species 

Pinning Down an Uncertain ID 

If the top-rated choices show any of the following results, you may 

want to do some additional probing: 

• The top-rated ID choices on the list are random and unrelated 

genera 

• For GN, GP, & AN database (24 hour results): 

• SIM is very near 0.5 

• DIST is greater than 5.0 

• DIST of the first and second choices are nearly equal (less than 

two distance points apart) 

• There are many variable reactions (more than 15) 

• For YT database (≥48 hour results): 

• SIM is near 0.5 


• DIST of the first and second choices are nearly equal 


• For FF database (all time points): 

• SIM is near cutoff values for each day (1-4) 


• DIST of the first, second and third choices are nearly equal 


Note: For FF MicroPlate identifications, verification of the culture 

morphology is necessary. Refer to the software database photo library 

or mycology textbooks as references. 

For additional assistance in interpreting results, contact Biolog 

Technical Service. 

ID Rules of Thumb: 

SIM = 1.0 � a perfect match 

SIM = 0.0 � no match 

The closer to 1.0, the better the match. 


Assess SIM for ID #1 

Gram-negative aerobes 

Gram-positive aerobes 

What Should I Do 

About “No 

Identification”? 

Check the 

on-screen + and – 

signs for that 

MicroPlate. You 

can use these 

mismatch 

indicators to 

assess what 

happened. 


1. The chart below will help you clarify your identification by using 

SIM and DIST values: 

USING SIM AND DIST TO ASSESS IDENTIFICATIONS 

� must be > 0.5 at 16-24 hours incubation 

� must be > 0.75 at 4-6 hours incubation 

Anaerobic microbes � must be > 0.5 at 20-24 hours incubation 

Yeasts � must be > 0.75 at 24 hours incubation 

� must be > 0.5 at 48 or 72 hours incubation 

Filamentous Fungi (& select yeasts) � must be ≥ 0.90 at 24 hours incubation 

� must be ≥ 0.70 at 48 hours incubation 



Assess SIM for top IDs 

If SIMs for top IDs are < the SIM required AND all belong to the same genus AND if their total is ≥ the SIM 

required, you can be confident that the microbe is in that genus. MicroLog will give you a genus-level ID 

rather than a species-level ID. 

Assess DIST for top IDs 

If SIM is OK, but DIST between ID#1 and ID#2 are 

very close 

If you remain unconfident of the ID, try to 

differentiate organisms by 

� Good match to both species (some are quite close) 

� Gram stain, colony morphology, oxidase and/or 

catalase tests, additional tests (see Bergey’s Manual) 

2. If you get a “No Identification” result, the % Prob value will not be 

displayed. When this happens, check for black + and – signs in the 

on-screen MicroPlate. 

• If there are both + and – random mismatches, this is probably a 

true mismatch. If so, this is probably a species not in Biolog’s 

database. You can add it to your own user-created database. See 

Section 8. 

• If the mismatches are all + or all –, you may have made a 

testing error. Refer to Table 6-1, and see Section 10. 

Table 6-1 will assist you in figuring out the cause of these mismatches: 



Mismatch Type What It Might Mean 

All + (your pattern is giving 

fewer positive reactions than the 

species you’re comparing it to) 

All – (your pattern is giving more 

positive reactions than the species 

you’re comparing it to) 

Under-inoculation 

A1 well is overfilled, contains clumps, 

or is cloudy 

Cells were mishandled, too old, cultured 

on wrong medium, suspended in wrong 

inoculating fluid, incubated at wrong 

temperature, etc. 

Over-inoculation (especially with 

enterics) 

A1 well is underfilled 

Contamination (mixed culture) 

TABLE 6-1. ASSESSING MISMATCHES 

FF database 

Perform a visual macroscopic and microscopic assessment of your 

isolate compared to the first three ID ranked species. Refer to 

MicroLog software photo library or mycology textbooks as references. 

Note: The A1 well is not used as a negative control in the FF 

database, therefore this well may not appear colorless. 

FF ID Workflow 

1. Read daily (at 24, 48, 72 hours) until ID is called. 

2. After an ID is called, verify the first three choice macro/microscopically 

3. If no ID, re-incubate the MicroPlate 

4. Read at 96 hours. If an ID is called, verify the first three choices macro/microscopically 

5. If no ID, launch additional investigation. Check first three choices macro/microscopically. 


When you’re 

interpreting 

results: 

1. Trust your 

eye over the 

reader. 

2. When in 

doubt, 

repeat the 

test, using 

stringent 

technique. 

GN, GP, and AN databases 

1. Perform a visual assessment by checking the A1 well. 


• If it contains a significant purple color, this color may be 

obscuring the pattern. Re-examine your general setup 

procedures. 

• The strain may need to be retested with thioglycolate added to 

the inoculating fluid. 

• With some rare gram positive species, salicylate must be added 

(in addition to thioglycolate) to eliminate the false positive 

purple color. 

2. Check for borderline reactions. The system essentially disregards 

these when making identifications. 

• Review and verify your testing procedures. Did you use all 

optimal conditions? 

• If there are more than 15 borderline reactions, visually check 

the MicroPlate. If you used the reader, compare how it read the 

pattern in relation to how you read it by eye. If you disagree 

with the reader, click the Set Up tab on the menu bar. Select 

Manual mode and correct reactions manually. Or you can use 

the histogram function to adjust the thresholds (see Section 5). 

Re-identify based on the modified pattern or incubate the 

MicroPlate for a longer time (do not exceed time limits). 

3. Check for a message in the ID box. When appropriate, the ID box 

will offer additional information to guide your identification. For 

example: 

• ID of Salmonella, Shigella, or E. coli O157, ID box message 

says “Confirm by Serology” 

• ID of Listeria species or Neisseria gonorrhoeae, ID box 

message says “Confirmation of ID is recommended.” 

Species Comparison 

MicroLog software has several features that can help with species 

identification. You can compare any pattern with the expected pattern 

of any species or taxa in the database. You can also display expected 

species patterns visually. 

To compare your #1 ID with others on the list of runners-up: 



What are those + 

and – signs? 

These are signs 

showing 

mismatches 

between your 

sample and the 

database record. 

+ > 80% pos 

1. After a pattern is entered, the on-screen MicroPlate automatically 

displays the major mismatches between the #1 ranked species and 

your MicroPlate. 

2. To compare your ID with other species on the list, click on the 

species you want to view. A small arrow appears next to the 

selected organism and the major mismatches are displayed. 

– < 20% pos Mismatches 

displayed 

PLUS (+) AND MINUS (–) MISMATCHES 

3. To compare against a species that is not in the 10 top-ranking, click 

on the Other row. See page 13 

Searching and Assessing Species Information 

MicroLog software gives you an opportunity to find out more about 

the range of reaction patterns of species in the MicroLog database (or 

any record in a user database). There are three possible ways to access 

the species information feature, all from the Data window: 

• Using the View Database button on the selection bar. 

• Using the ID box 

• Using the “Other” row 

Using the View Database Button 

You can get advanced species information without running 

identification, for single or multiple records in any database. 



1. Click the View Database selection bar on the Data window. The 

Database List appears. This is a species list from the database, 

based on the strain type you last selected on the Data window. 

2. Click the Database Options tab to select the database you want to 

view, based on strain type. 

3. The Database List contains 15 records per page. Click Page Up or 

Page Down or use the scroll bar to move through the list. 

4. Click on the row containing the species you want. 

5. This will take you to the End-Point and Progressive data pages. 

DATABASE OPTIONS WINDOW DATABASE LIST WINDOW 

Endpoint data analysis 

For each well, the number displayed represents the percentage of strains of 

the displayed species that were positive at the selected incubation time. 

1. In the ID box in the Data window, right-click on the species you 

want to investigate. The End-Point window in the Database Data 




NOTE: A1 

value may be 

greater than 0 

if the organism 

tends to give 

false positive 

reactions. 

YT & FF 

MicroPlates are 

limited to endpoint 

data 

analysis 

only 

Progressive data 

analysis applies to 

bacterial 

identifications 

only 

DATABASE DATA WINDOWS (END-POINT WINDOW) 

2. The numbers appearing in the on-screen MicroPlate show (for each 

well) the average “percent positive” value, considering all strains 

of the species that were tested. This is a simple way to view 

species data. 

3. Click the Progressive tab to view more sophisticated bacterial 

species data, namely, the range of reactions you can get for this 

species as the pattern develops in a progressive fashion. 


On all progressive 

data windows: 

Click on a specific 

well to select it. 

Find well 

information in 

“Key” box. 

Click “Set to 

Current Data 

Value” to return 

all settings to 

number of positive 

reactions your 

current 

MicroPlate has. 


Progressive data analysis 

MicroLog software uses a new method of pattern matching called 

Progressive ID (PID). The PID method takes into account the 

progressive nature by which purple color forms in the wells of the 

MicroPlate. What typically happens after cells are inoculated is that 

some wells turn purple quickly and strongly, resulting in dark purple 

wells. These are usually the preferred carbon source for the microbe. 

After additional incubation, other wells also turn purple, sometimes 

forming weaker purple wells. 

For example, if a MicroPlate has 23 purple wells, PID will compare 

the pattern to the other species in the database at their stage of 

development when they also had 23 purple wells. Each species in the 

database responds to the search, as if to say “This is my pattern when I 

had 23 positive reactions.” PID finds the best match among the species 

in the database. The mathematics of matching the species to the pattern 

is otherwise identical to that used in the previous method of End-point 

ID (EID). 

Reaction range 

This display shows the entire metabolic progression of the species in a 

single image. The boxes are entirely purple when the well is positive 

100% of the time. A teal color indicates that the well is positive from 

20% to 80% of the time. Empty boxes indicate that the substrate is 

used < 20% of the time. Each box contains a black line that shows how 

many positive reactions are on the given MicroPlate. This black line is 

a gauge of progressive identification. The top right corner of the 

window contains a key showing the current reaction of a specific well 

and how often it is positive. 

REACTION RANGE WINDOW 



Slide the scroll bar left and right to represent various levels of positive 

reactions. As the vertical line moves across each well of the on-screen 

MicroPlate, it indicates where in the histogram a chosen positive well 

value falls. 

• The purple area under the graph (histograms) represents when a 

well is positive for that species. 

• A box that is completely purple is always positive for that species. 

This is a preferred carbon source for that species. 

• A box that has limited levels of purple is only positive when there 

are relatively high numbers of positive wells on the MicroPlate. 

• Teal represents borderline well reactions. 

• You can click on any well and it will appear as the example shown 

on the key at the top. 

Specific pattern 

This display shows the numerical pattern for the species at a specific 

level of positive reactions. This is a more explicit display of the 

Reaction Range display. For the number of positive reactions 

(indicated below the display and adjusted by using the scroll bar), this 

is the percentage of times that a specific well is positive. 

• A well that is 100 when the scroll bar is far to the left is a well that 

is always positive for that species, even when it has few positive 

reactions. 

• A well that is only 100 when the scroll bar is to the far right is a 

well that is only positive when the species has many positive 

reactions. 

• A well that contains a low number or zero is a well that is rarely or 

never positive, even when the species has expressed its full 

metabolic potential. 


SPECIFIC PATTERN WINDOW 


Vs. current MicroPlate 

This display shows a graphical representation of the pattern for the 

species at a specific level of positive reactions versus the MicroPlate 

currently on the Data window. The circle at the left of each box 

represents the reactions in the current MicroPlate. The bar at the right 

of each box represents the percent of times the species is positive at 

the given level of positive reactions. 

• Slide the scroll bar left and right to see the animation of the 

metabolic progression for that species. 

• Purple indicates positive wells. 

• Teal represents borderline well reactions. 

• Empty boxes are always negative. 

• The display shows major mismatches between the current 

MicroPlate and the species, at the current level of positive 

reactions. 

• If you set the number of positive reactions to be equal to the 

number of positive reactions in the current MicroPlate, you can see 

how the ID search treats the match between your MicroPlate and 

the displayed species. 



VS. CURRENT MICROPLATE WINDOW 

Using Macroscopic and Microscopic Pictures 

You can access macroscopic and microscopic species of selected fungi 

in the MicroLog photo library of the FF database. 

1. From the Data window, click the View Database selection bar. 

The Database List appears. This is a species list from the database 

based on strain type last selected on the Data screen. 

2. Click the Database Options tab and select the database you want 

to view, based on strain type. 

3. Click back to the Database List. Click Page Up or Page Down or 

use the scroll bar to move through the list. 

4. Click on the row containing the species you want. 

5. The End-Point, Micro Pictures, and Macro Pictures tabs appear. 

6. Click on the Macro or Micro tab to access the photo library. 

MACRO AND MICRO TABS ON DATABASE DATA WINDOW 


MACROSCOPIC PICTURE 

MICROSCOPIC PICTURE 


Using the Other Row 

You can use the Other row at the bottom of the Data window to get 

additional species information. 

1. Double-click anywhere on the Other row at the bottom of the ID 

box (watch for the small arrow). 



“Other” field 

2. The Database List appears. Click on the row containing the 

species you want to highlight it. 

3. Click OK to return to the Data window. Note that your selection 

now appears in the Other row. In addition, positive (+) and 

negative (–) mismatches appear in the on-screen MicroPlate, as 

does the DIST value in the ID box. This performs a comparison 

between the database record and your MicroPlate ID. 

Note: If you are searching both the MicroLog and User databases, the 

program will request that you designate which database you wish to 

compare to before the Database List appears. 

4. Click on the Other row and press Enter or Backspace on your 

keyboard to clear the entry. 

DATA WINDOW WITH “OTHER :FIELD FILLED (NOTE DIST AND +/– ENTRIES) 

Accessing Raw OD Values 

This function allows you to access the raw OD values for the 

wavelengths read. 

1. Click the Raw Data tab on the Data window. This tab is located 

below the reaction section of the Data window. The raw OD 

values for the wavelengths read will be displayed. 

2. To return to the previous data view, click the Data tab. 

Raw data tab 



Accessing Data Values 

This function allows you to access the Data values for the wavelengths 

read. This is useful to view both sets of data values for the FF results 

on one screen. 

1. Click the Data Stats tab on the Data window. This tab is located 

below the reaction section of the Data window. The Data values 

for the wavelengths read will be displayed. 

2. To return to the previous data view, click the Data tab. 


Data Management and Compiling Databases 

7. Data Management and Compiling 

Databases 


�BackUp a 

Data File 

�Combining 

Data Files 

�Viewing 

and Editing 

Files 

�Compiling 

Data Files 

�Exporting 

ASCII Data 

�Cluster 

Analysis 

The heart of the software is a library of over 1,900 microbes, 

distributed over a number of organism databases. You can replace or 

augment this library with other species or strains. The user can 

compile an ID File (customized database) from a Data File and 

perform identifications using either our database or your own 

customized database. The software also provides functionality for 

managing Data Files, which is also useful in preparing a Data File for 

compilation. These functions include backup (copying), combining, 

editing, printing, exporting to other applications (such as Microsoft 

Excel® or Microsoft Access). 

The software also provides dendrograms and Multi-Dimensional 

Scaling diagrams, useful for cluster analysis. 

DATA FILE DATA MANAGEMENT MENU PAGE 

Data File Management under Restricted Access mode 

If the software is running under Unrestricted Access mode, then any 

user may access the Data File Management page. Under Restricted 

Access mode, only users with at least the View/Print privileges may 

have access. If a user with the Log-In and Set Up privileges only, then 

a warning message box pops up stating that the user: “Must have the 

View/Print privilege to navigate to the Data File Management page”. 

Users with the View/Print privilege may access the following features: 

• Backup Data File 



If desired, 

view the 

destination 

/edited file 

using the 

View/Edit 

feature to 

confirm 

data file 

modifications. 

• View and Print functions (from the View/Edit Data File 

button) 

• Export to ASCII File 

Users with Edit Data File privileges have access to advanced features, 

including: 

• Add File to Data 

• Editing functions (from the View/Edit Data File button) 

• Compile/View Data File 

Backup Data File (Copying a Data File) 

Before altering a Data File in any way, it is good practice to make a 

back-up copy of the Data File. Frozen files cannot be edited; therefore, 

a backup copy must be created on which to perform any editing. The 

backup copy of the Data file keeps track of the file from which a 

record was copied (parent file). This creates an electronic trail of the 

record. 

1. Click the Data File tab on the menu bar to view the Data 

Management page. Click on the Data Management File field and 

a Data File dialog box appears which lists available Data files. To 

select the file, click on the name of the file. 

2. Click Open. 

3. Click the Backup Data File button to view the BackUp Data File 

save dialog. 

4. Enter the new desired file name (backup copy) into the File name: 

field. You can stay in the same directory as the original or 

navigate to another directory to save the file. 

5. Click Save. 

. 

BACKUP DATA FILE SAVE DIALOG 


Combining Data Files 


1. Click on the Data Management File field and a Data File dialog 

box appears which lists available Data files. Click on the name of a 

non-Frozen Data File (non-original data file or backup data file) to 

select that file as the destination file. 


3. Click the Add File to Data File button to view the Add Single 

Files dialog. Note: Destination file is listed in the Old File section. 

4. Click in the Source File section to view the Find New Data File 

open dialog. 

5. Click on the desired data file name (file to add). 

6. Click Open. Note: Last selected file is listed in the New File 

section. 

7. Click the Add Source to Target button. The file you’ve added 

will be listed in the ‘Files Added This Session’ section. The file 

listed in the Target File box now also contains a copy of the file 

listed in the Source File box. (The Source File box still contains 

the name of the added file until another file is selected.) 

8. Follow steps 4 thru 7 to add additional files. 

Note: As a safety feature(to avoid spurious duplication of records), the 

software will not allow you to add a file more than once in one 

session. However, if the user closes and re-opens this dialog, then the 

software does not prevent the user from adding a file that may have 

already been added. 

9. To freeze this file after you have added to it, click Freeze Data 

Records at the bottom of the Data Management window. 



Viewing and Editing Data Files 

1. Click on the Data Management File field and a Data File dialog 

box appears which lists available Data files. Click on the name of a 

Data File to select that file (non-original data file or backup data 

file). 


3. Click the View/Edit Data File button to view the Data File Edit 

dialog. Note: Dialog contains a spreadsheet, in which each record 

corresponds to a read of a plate. The dialog is initially in the View 

Only mode. 

VIEW/EDIT DATA FILE DIALOG 

4. You will be in View Only mode. You can examine the records, but 

not edit them even if the file is not frozen. If desired, navigate to 

other columns or rows by using the horizontal or vertical scroll bar 

or adjust the column widths by carefully positioning the cursor 

between column headings until the cursor icon changes and, 

subsequently, dragging the cursor. 

5. Select the Print tab to access the print options. 

CLOSE-UP OF PRINT OPTIONS IN DATA FILE EDIT DIALOG 


OK, Out, Hold, 

Atypical 

Sorting labels 

may be applied to 

individual 

identification 

records. 

This sorting 

facilitates the 

task of 

organizing 

records within in 

a datafile for the 

compiling user 

databases. 


• Click desired radio button to select ‘Text’ or ‘Graphics’ as 

the Print Mode. 

• Click desired radio button to select a Print Selection option: 

‘Print Record’ allows printing of the selected record, which 

is the record with an arrow icon in the row heading. ‘Print 

Page’ allows printing of all the records that appear on the 

spreadsheet. ‘Print All’ allows printing of all the records in 

the Data File. 

• Click the Print Fields button to print the ‘information’ fields 

that are in the spreadsheet. 

• Click the Print Results button to print each identification 

report printout. 

6. Select the Edit In/Out tab to label a record (used in the Data File 

compilation feature, Sec. 7 page 7). Users without the Data File 

Edit privilege are prevented from accessing this page. 

CLOSE-UP OF DATA FILE COMPILATION OPTIONS IN DATA FILE EDIT DIALOG 

• Click desired radio button to select a ‘Prompt Before Edit’ 

option. Note that the ‘Prompt’ option will prompt the user for 

an OK button click before applying any edit. 

• Click desired radio button to select an Edit Mode option: 

OK (to include a record in compilation) 

Out (to exclude a record from compilation, 

while indicating that it will never 

become eligible in the future) 

Hold (to exclude a record, while indicating 

that it may become eligible in the future) 

Atypical (to exclude a record, while indicating 

that it may become eligible on a 

contingency basis) 

• Once you have selected to Edit Mode, click on the row to label 

the record in the spreadsheet. Note that the same edit may 

rapidly be applied to many records by clicking many records in 

succession. 



7. Select the Edit Fields tab to access options that allow rapid editing 

of many records. Note that users without the Data File Edit 

privilege are prevented from accessing this page. 

CLOSE-UP OF EDIT FIELDS OPTIONS IN DATA FILE EDIT DIALOG 

• Click radio buttons to select a field for editing from among the 

Data Field options. 

• Enter the value of the selected field into the Edit box. 

• Once you have selected the Data Field and entered the value 

(new), click on the row on the spreadsheet to edit (replace 

value). Note that the same edit may rapidly be applied to many 

records by clicking many records in succession. 

8. Select the Edit Values tab to access options that allow extensive 

editing of a single record. Note that users without the Data File 

Edit privilege are prevented from accessing this page. 

• Click on a row in the spreadsheet to view a Plate Information 

dialog for the record corresponding to the selected row. 

• Enter a value into or change a value in each Edit box, as 

desired. Select a different value in each Pull Down box, as 

desired. 

• Click Save, Done or Cancel. 

9. Select the Copy tab to access options that allow copying of 

selected records into another Data File. Note that users without the 

Data File Edit privilege are prevented from accessing this page. 

CLOSE-UP OF COPY OPTIONS IN DATA FILE EDIT DIALOG 

• Click desired radio button to select a Mark Record option: 


Remember! 

When building 

your own 

databases, strain 

names must be 

consistent when 

you group 

patterns. Using 

inconsistent strain 

names will result 

in incorrect 

database 

groupings. 


Mark ( to select a record for copying ) 

Clear ( to de-select a record for copying ) 

• Once you have selected the Mark Record option, click on 

the row to label the record in the spreadsheet. Note that the 

same edit may rapidly be applied to many records by 

clicking many records in succession. 

• To mark or clear a large numbers of records 

simultaneously, click one of the following buttons: 

Mark All (to select all the records in the Data File) 

Mark OK’s (to select for copying only those records 

that are also ‘OK’ for compilation) 

Clear All (to de-select all records ) 

• Click the Write Marked Recs to New File button to view the 

Save Marked Data to this File save dialog. 

• Enter the desired file name of the copy into the File name: 

field. Click the Save button. 

10. Click Freeze Data Records if you’ve finished editing the file and 

want to save it as a “Read Only” file. This will create a file that is 

non-editable until another backup copy is made (Backup Section 7, 

page 2). 

Compiling Data Files 

The software also allows you to compile data files and produce 

databases. 

Producing databases 

Be sure to save GN, GP, AN, YT, and FF MicroPlate-type readings in 

separate files for database compilation. Certain features of the 

compilation menu are specifically linked to MicroPlate type. 

Choose the existing file to compile by clicking on the Data 

Management File field on the Data Management window. A Data 

File dialog box appears. 

1. Click on the desired file name. 


3. Click the Compile/View Data File selection bar on the Data 

Management window. The Compile/Index/Data tabs appear, 

defaulting to the Compile window. 



4. In the Data To Include section, enable check marks in the desired 

box(es). (See pages 7.4-7.6 for the Edit In/Out process). 

OK (to include records designated OK) 

Hold (to include records designated Hold) 

Atypical (to include records designated Atypical) 

Out (to include records designated Out) 

6. In the What Processing To Do section: 

• Enable the check mark next to Make Index Files (this must be 

enabled to use the rest of this feature below). 

• Enable Make ID Files to make your own database. 

• If you are compiling files with GN, GP, or AN plate types, the 

window below opens. 

COMPILE/INDEX/DATA WINDOWS (FOR GN/GP/AN PLATE TYPES) 

• If the file you are compiling contains YT or FF MicroPlate 

data, you must specify the incubation time for the data you are 

compiling. If the file contains several incubation times, select 

All. The software will compile the entries by strain name and 

automatically make an endpoint database for each incubation 

time. For further information, follow the on-screen instructions. 



COMPILE/INDEX/DATA WINDOWS (FOR YT/FF PLATE TYPES) 

• Click Go to compile database. 

• Indication bars at the bottom of the window show compilation 

progress. 

• The Progressive database file will have the same name as your 

data file, with a “.KID” extension (GN,GP, and AN files) and 

the Endpoint database file, with a “EID” extension (YT and FF 

files). The user databases are saved as read only files. 

7. Click the Index tab on the Compile/View Data File window to 

show the Index window. 

• You will be able to view a data file list by Sample Number, 

Strain Name, or Strain Number. Click the desired radio 

button and the list will change accordingly. 

• Click Page Up or Page Down or use the scroll bar to view the 

entire list. 

• To view an entry more closely, click on a row, then click the 

Data tab at the top of the page. 



INDEX WINDOW OF COMPILE/VIEW DATABASE (WITH SAMPLE ENTRIES) 

• The data spreadsheet will show you all information for that 

sample. You can perform all functions (such as editing and 

printing) from the View/Edit Data File menu. 

• If you edit your data file after compilation, you must recompile the 

data before using the database to identify organisms. 

• Click OK to close. 

DATA WINDOW OF COMPILE/VIEW DATABASE 

8. Click on the Setup tab to view the User Database you created. 

• Click on the Database tab. 

• Click on the Database to Search pull-down list. 


DATABASE WINDOW 


• Select User. 

• If the database contains GN/GP/AN plate types, click on 

the User Progressive Database field. 

• If the database contains YT/FF plate types, click on the 

User End-point Database field. 

• A Progressive or Endpoint Database File dialog box 

appears. 

DATABASE FILE DIALOG BOX 

• Select the user database you want. 

• Click Save. 

• Freeze and/or comment on the database file when you 

finish editing. 

• Click on the Data tab. 



User 

database 

development 

To evaluate 

various database 

versions during 

development, the 

Re-ID function 

allows you to 

perform ID test 

evaluations on 

test backup data 

files. 

!Note: Use of 

the compile tab 

re-ID feature 

is not 21CFR 

part 11 

compliant. 

• Select the appropriate Plate Type, Strain Type, and 

Incubation time for the User Database. 

• Click on the View Database bar under the picture of the 

MicroPlate. 

• A list of organisms in your User Database appears similar 

to a MicroStation/MicroLog database list. 

Compile tab Re-ID feature 

1. Choose the existing backup file for User database development and 

analysis to Re-ID by clicking on the Data Management File field 

on the Data Management window. A Data File dialog box 

appears. 



4. Click Compile/View Data File. 


• Enable the check mark next to Make Index File (this must be 

enabled to use the rest of this feature). 

• Enable Re-ID Data identify your data against a database you 

have created or re-threshold data collected in an earlier 

software release to the thresholds in the current release. 

• Click on the Re-ID Database field to select the appropriate 

database. The software will re-identify the data file based on 

this entry. 

• If the file to Re-ID contains GN, GP or AN MicroPlate types, 

the appropriate progressive database is selected. 

SELECT RE-ID DATABASE WINDOW 



• If the file to Re-ID contains YT or FF plate data the with only one 

incubation time, choose the corresponding endpoint database file 

for that time in the Re-ID Database field. For example, if the FF 

file is from 24-hour incubation time in Step 1, choose 1 Day. For 

the Re-ID Database, select FF60124.eid (24 Hour). 

SELECT RE-ID DATABASE WINDOW 

• If the YT/FF file contains several incubation times, select All 

at Step 1. The software will compile and Re-ID the entries by 

strain name and incubation time. You do not need to select a 

database for the Re-ID Database field. 

• Click Go to Re-ID the file. 

• Indication bars at the bottom of the window shows the Re-ID 

progress. 

• Click OK when the process is finished. 

Printing data headers, species counts and dendrograms 

These features are automatically printed once they are compiled. The 

software does not save a permanent record. 

1. Choose the existing file to compile by clicking on the Data 

Management File field on the Data Management window. A 

Data File dialog box appears. 




• Enable the check mark next to Make Index File (this must be 

enabled to use the rest of this feature). 



Re-ID using 

input mode 

features 

You must use 

this method to 

re-ID previously 

run data against 

your user 

database and 

save as a new 

file to be 21 

CFR part 11 

compliant. 

Select file name here 

• Enable Print Ranked Data Headers if you want to print a list 

of all the information in the data file as entered in a spreadsheet 

row, ranked by MicroPlate type and species (see Appendices 

for sample printout). 

• Enable Print Species Counts if you want to print by strain 

type, one row per species, with species name and number of 

MicroPlates for that species (this will give you a species count) 

(see Appendices for sample printout). 

• Enable Print Species/Plate Dendrograms if you want to 

generate a dendrogram printout, with each dendrogram entry 

equaling one MicroPlate. The same species must have at least 

three entries in the file to make a dendrogram. Each 

dendrogram is determined by the strain name (see Appendix 5 

for sample printouts). The dendrograms display linear 

relationships. 

Entering Reactions from a Saved File for Re-ID 

1. Use the Data Input Mode drop-down list to select File (to re-ID 

saved data records from a saved file) 

2. The Input Data File Name field appears. Click in the empty field 

and move through the listed folders to find the file you want to 

read and click on it. This will bring the file name up on the Set up 

screen. 

SET UP WINDOW (WITH FILE MODE SELECTED) 


Arrow 

indicates 

selected 

entry 


3. In the Write Data to Data File drop-down list 

a. Select Yes to save the generated data to another file. 

b. Click on the Output Data File Name field. A Save As 

dialog box appears. Enter the desired file name (D4C 

extension). 


5. Click Select Read 

6. A list of saved data appears. Select the MicroPlate you want to 

read and click OK. 

LIST OF SAVED DATA 

7. The Data page will show the MicroLog ID record. 

a. Click the Setup tab. 

b. Click the Database tab. 

i. Select User. 

ii. Enter database file name, as required for User 

database search options. 


a. The re-ID based on the MicroLog database is in view. 

b. Click the Setup tab. 

c. Select Manual input mode. 

d. Click the Data tab. 

e. Click on Re-read button 

f. Manual entry display appears with the reactions populated. 

g. Click OK to re-ID. 

h. The Data screen is displayed with the re-ID result. 

i. Click Save to save the ID generated for the file record 

entered to your Output file. 



9. Click the Setup tab. 

10. Use the Data Input Mode drop-down list to select File 


12. Click Read Next (to select the next record) or click Select Read 

(to choose another record) and click OK after the selection. The 

program defaults to the MicroLog database in file input mode. 

13. Repeat steps 7 thru 12 until you have re-ID’d all of the records that 

you wish. Click Save to save the ID generated for the file record 

entered to your Output file. 

Exporting ASCII Data 

The software enables you to export data into an ASCII file, and export 

data to Excel and/or Microsoft Word. 

Exporting data into an ASCII file 

1. Choose an existing file to export to by clicking on the Data 

Management File field on the Data Management window. A 

Data File dialog box appears. 



4. Click the Export to ASCII File selection bar on the Data 

Management window. The Export to ASCII File window 

appears. The file you chose appears in the Data File To Give Data 

field. 

ASCII EXPORT WINDOW 



5. Click on the ASCII File to Get Data field. An ASCII Export File 

dialog box appears. 

ASCII EXPORT FILE DIALOG BOX 

6. Use the Save as type pull-down list to select the format of the 

ASCII file you want to import. 

• Text File shows a list with a .prn or .txt extension. 

• Comma Delimited shows a list with a .csv extension. 

• All Files shows a list of all files 

7. Type in the desired name for the ASCII file where you want to 

export the data or click on a previously created ASCII file to 

append to it. Be sure to use the correct file extension. 

8. Click Save. The ASCII Files window reappears. 

9. Choose Type of Format, click the radio button designating the 

format you desire for your ASCII file. 

• One-Line, Comma Delimited (see Section 7, page 17) 

• Line Delimited, Data 8x12 Space Delimited (see Section 7, 

pages 18-19) 

• Fixed 8x12 O.D. Format 

• Comma Delimited Files for Importing into MicroSoft’s 

ACCESS database software 

10. Click the Format tab or Go To Format Page. The Export to 

ASCII File window appears. The choices on the Data Fields list 

depend on the MicroPlate in the file you’ve chosen. For a 

definition of each of the Data Fields (refer to Section 7, page 20 

lists). 



data file 

field 

information 


(GN,GP,AN,YT) (FF) 

ASCII EXPORT WINDOWS 

11. If necessary, click Clear All Fields to clear fields from the right 

list. 

12. Click Add All Fields to select all data fields for ASCII File Fields. 

13. Click on the left (data) field you want, then click on the next empty 

row on the right (ASCII) field. This information will be exported 

from the ASCII file. Add all the desired information. 

Note: Clicking on a previously used row inserts a field. 

14. Click Remove Field and then the ASCII File Fields field to 

remove a selected Data Field. 

15. Click OK. The Files window reappears. 

16. Click Convert Data File � ASCII File. 

17. Click OK to exit. 

18. Minimize the MicroStation/MicroLog software. 

19. Open the software where you wish to view the exported data. 

Exporting to a .csv file for EXCEL 

1. Choose Comma Delimited as the Save as Type at Step 6 in the 

procedure above. 

2. Choose One Line, Comma Delimited at Step 9 above. All fields 

selected for export will be in one line across the columns of the 

EXCEL spreadsheet. Data Field titles will also be exported. 



3. Open EXCEL after you have exported the desired data and 

minimized the MicroStation/MicroLog software. 

4. Click on File � Open. Under Files of type, choose Text Files. 

5. Move through the folders to the C: drive. Open the Biolog folder 

and look for the file where the data was exported 

Note: The file extension may not appear, but you will see the EXCEL 

symbol on the icon next to the file. 

6. Click on your file name. The file will open and data will appear 

with Data Field Headers. 

7. Select Format from the toolbar. 

8. Select Column. 

9. Select Auto Fit Selection. 

Note: The instructions for opening the file in EXCEL may vary 

slightly, depending on the version you’re using. 

Exporting as a .txt or .prn file for EXCEL 

Note: Use this to export reaction results in an 8x12 format similar to 

software printouts. 

1. Choose Text File as the Save as type at Step 6 in Section 7, page 

15. 

2. Choose Line delimited, Data 8x12 Space Delimited at Step 9 in 

Section 7, page 16. All fields selected for export will be on 

separate lines on the EXCEL spreadsheet. Data Field titles will not 

be exported. 

3. Open EXCEL after you have exported the desired data and 

minimized the MicroStation software. 

4. Click on File � Open. Under Files of type: choose Text Files. 

5. Move up through the folders to the C: drive. Open the Biolog 

folder and look for the file where the data was exported 

Note: The file extension may not appear, but you will see a text icon 

next to the file. 

6. Click on your file. A Text Import Wizard box appears. 



7. Choose file type as Delimited. 

8. Click Next. 

9. Under Delimiters, deselect Tab and select Space. 

10. Click Next. 

11. Click Finish. 

12. Your file will open with all the Data Fields on separate lines. If 

you chose to export any +/- values or OD values they will be in an 

8x12 format. 

Note: The instructions for opening the file in EXCEL may vary 

slightly, depending on the version you’re using. There are no Data 

Field Headers for each field exported. 

Exporting as a .txt or .prn file for Microsoft Word 

Note: Use this option to export reaction results in an 8x12 format 

similar to software printouts. 

1. Choose Text File as the Save as type at Step 6 in Section 7, page 

15. 

2. Choose Line Delimited, Data 8x12 Space Delimited at Step 9 in 

Section 7, page 16. All fields selected for export will be on separate 

lines on the EXCEL spreadsheet. Data Field titles will not be 

exported. 

3. Open Microsoft Word after you have exported the desired data and 

minimized the MicroStation/MicroLog software. 

4. Click on File � Open. Under Files of type: choose Text Files. 

5. Move up through the folders to the C: drive. Open the Biolog 

folder and look for the file where the data was exported. 

Note: The file extension may not appear, but you will see a text icon 

next to the file. 

6. Click on your file name. A File Conversion box appears. 

7. Choose Plain text as the encoding loading this file. 

8. Click OK. 


Export Help 

Mark this choice 

if you wish to 

export to Access. 


9. Your file will open with all the Data Fields on separate lines. If 

you chose to export any +/- values or OD values they will be in an 

8x12 format. 

Exporting to Microsoft Access 

You may also wish to export data to Microsoft Access. This can be 

done by clicking the Export to ASCII File button on the Data File 

window. In the Step 2 box mark the comma delimited for Access 

format. For further information on exporting into Access, please see 

the Help tab on the Export to ASCII File window (see below). 



ASCII Export Window Entries 

• Date: Date data was saved 

• Plate Type: GN, GP, YT, FF, etc. required and selected from saved file 

• Plate Number: number you designated for plate information 

• Incubation Time: time you designated depending on MicroPlate type 

• Sample Number: number you designated for plate information 

• Strain Type: GN-ENT, or GN-NENT, etc., determined by the MicroPlate type 

• Strain Name: name you designated for plate information 

• Strain Number: number you designated for plate information 

• Other: information you add to further describe sample 

• “+/b/-“ Reactions: results from reading MicroPlate displayed as: 

o “+” = positive 

o “-“ = negative 

o “b” = borderline 

• 1/0.5/0 Reactions: OD display of results if reactions read manually 

o 1 = positive 

o 0 = negative 

o 0.5 = borderline 

• Dual Wavelength Data (DWD): O.D. data on printouts if GN, GP, AN, MT or Eco plate is read from a MicroStation 

Reader = [(590nm–750nm) x – (590nm-750nm) A1] X 1000, where x=any well 

• Dual Wavelength O.D. (DW): OD difference data if GN, GP, AN, MT, or Eco plate read from a MicroStation 

Reader = (590nm–750nm) x, where x=any well 

• 750nm O.D.: Raw O.D. read at 750 nm from MicroStation Reader 

• 590nm O.D.: Raw O.D. read at 590 nm from MicroStation Reader 

• 590nm %: O.D. data on printouts if YT, SFN, or SFP plate read from a MicroStation Reader= 

• 590nm % = (590nm x – A1) x 100, where x=any well 

• 590nm Filter Position: position 5 

• 750nm Filter Position: position 6 

• Data Generation Mode: Manual or Reader 

• ID Result: Message if there is NO ID or Genus species name if ID obtained from reading MicroPlate 

• ID #1 Name: Genus species name of organism listed as first choice on printout of results 

• ID #1 SIM: SIM of organism listed as first choice on printout of results 

• ID # 1 DIS: DIS of organism listed as first choice on printout of results 

• ID #2 Name: Genus species name of organism listed as second choice on printout of results 

• ID #2 SIM: SIM of organism listed as second choice on printout of results 

• ID # 2 DIS: DIS of organism listed as second choice on printout of results 

• ID #3 Name: Genus species name of organism listed as third choice on printout of results 

• ID #3 SIM: SIM of organism listed as third choice on printout of results 

• ID # 3 DIS: DIS of organism listed as third choice on printout of results 

ASCII Export Window Entries (Unique Entries for FF) 

• Color Data: O.D. Data on FF printouts for color data = 

• [(490nm x –490nm A1) -(750nm x –750nm A1)] X 1000, where x=any well. 

• Turbidity Data: O.D. Data on FF printouts for turbidity data = 

• (750nmx –750nm A1) X 1600, where x=any well. 

• Dual Wavelength O.D. (DW): OD readings at 490 nm and 750 nm minus the A-1 well: 

• (490nm x - 450nm A1), where x=any well. 

• (750nm x - 750nm A1), where x=any well. 

• 750nm O.D.: Raw O.D. read at 750 nm from MicroStation Reader. 

• 490nm O D: Raw O.D. read at 490 nm from MicroStation Reader. 

• Color Filter Position: 490nm, position 3 

• Turbidity Filter Position: 750nm, position 6 



Cluster Analysis 

Cluster analysis uses a mathematical approach to display the relative 

similarities and differences between strains or species based on pattern 

matching. It offers a visual method for examining groups using linear 

dendrograms and two- and three-dimensional cluster diagrams. You 

can generate cluster analyses on groups of entries in Biolog’s database 

or your own database. 

The math behind cluster analysis 

Dendrograms are generated using a modified UPGMA analysis 

developed by Biolog (algorithm for constructing rooted phylogenic 

trees). The algorithm uses DIST values to generate the branching 

structure of the dendrogram. A DIST value of 14 is usually used as a 

cutting level to separate genera. This value has been empirically 

determined to be an appropriate one for most genera of gram-negative 

bacteria. 

Once the software generates a preliminary dendrogram, it makes a 

second pass, rotating all branch points to their most preferred position. 

This best maintains proximity of related strains or species. If the 

distance between strains or species adjacent to cut points is less than 

14, they are joined with a dashed line that indicates their actual 

distance. 

By mathematically removing the linear constraints of the dendrogram, 

the software can generate two- and three-dimensional diagrams of 

DIST relationships. 

All of these cluster diagrams are only approximations of true 

relationships. With 95 carbon source tests, it would take 95dimensional 

space to provide fully detailed clustering. As a practical 

matter, the linear dendrograms and two- and three-dimensional 

diagrams give very satisfactory representations. 

Using cluster analysis with IDs 

1. Once you get a species ID, click the Cluster Menu selection bar on 

the Data window. 



Dendrogram 

numbering: 

�The 

number in ( ) 

is one less that 

the 

corresponding 

number found 

in the data file 

record. 

�The 

numbering 

here begins 

with 0 

�Data file 

numbering 

begins with 1 

2. The screen shows a dendrogram of your ID. 

CLUSTER MENU (TEXT) 

3. Click on the black line below or above the dendrogram to scroll. 

4. Click the Cluster Menu (Graphics) tab to view one-dimensional, 

two-dimensional, and three-dimensional cluster analyses. 

ONE-DIMENSIONAL (1-D) CLUSTER ANALYSIS 



TWO-DIMENSIONAL (2-D) CLUSTER ANALYSIS 

THREE-DIMENSIONAL (3-D) CLUSTER ANALYSIS 

5. Design your cluster according to your preferences. 

• Select B/W for a black and white representation. 

• Select Copy to paste your cluster into a text document (print 

screen). 

Note: Copy to Paint and use the cut and paste feature to place a 

“cluster only” picture into a Word document. 

• Select Color to choose the color scheme you want. 

• Select Big to enlarge your species. 

• Select Small to shrink your cluster. 



• Select In to zoom in on cluster. 

• Select Out to zoom out on cluster. 

• Select Distance to designate the distances between organisms 

with lines and to note the distances with a key in the lower 

right corner of the cluster. 

• Select Groups to encircle the entries that are in the same 

groups in the dendrogram. 

• Select Lines in the three-dimensional cluster to draw a line 

from each organism to the base. 

• Select to the left of Live to enable the spin of the threedimensional 

cluster left. 

• Select to the right of Live to enable the spin of the threedimensional 

cluster right. 

• Click on Spin, Roll, and /or Rotate, Fast or Slow, to capture 

the best view of your cluster. 

6. Click Print to print any diagram. (Note: “Live” feature must be 

disabled to print cluster diagram) 

Note: When searching your own database, you can view and print 

dendrograms, 2-D, and 3-D cluster diagrams by following the 

instructions above in “Using cluster analysis with IDs.” 

Using cluster analysis with collected data 

To obtain a dendrogram displaying the spatial relationships between 

your own organisms in a saved data file, refer to “Printing Data 

Headers, Species Counts, and Dendrograms” beginning on page 7-13. 

1. From the Compile window, enable the desired box: 

• Make Index Files 

• Print Species/Plate Dendrograms (to generate one 

dendrogram for each MicroPlate) 

2. When the data file compiles, printouts of your dendrograms will 

display linear relationships. 


8. Administration and Security 


� What Is Restricted 

Access Mode? 

� First Log-In and 

Setting Up an 

Administrator 

� Administrator 

Options 

� Viewing the Log-In 

Log 

� Changing or Lost 

Passwords 

� Unauthorized Log- 

In Attempts 

Administration and Security 

The software runs in Restricted or Unrestricted Access Mode, a term 

that may be familiar from experience with the OmniLog® System. 

Let's review what Restricted Access Mode does and why it is 

important. 

What is Restricted Access Mode? 

Restricted Access Mode requires that only users with Administrator 

privileges have the ability to oversee and control access to the 

software. 

The Administrator will: 

• Assign Usernames and passwords for those who will use the 

system. 

• Assign access privileges to each user. 

• Oversee the security of the system 

Restricted Access Mode requires all users to Log-In with a Username 

and password when entering the software or changing users. The 

software maintains files of the User List, Log-In Log and Log-In 

Log Archive to keep track of registered users, access privileges, and 

Log-In/out activity in the system. 

All of these files are encrypted. The Log-In Log Archive files can be 

placed by the Administrator in the computer/network location of their 

choosing. This allows the Administrator to place the files in a location 

that has secured access. 

Why Restricted Access Mode? 

Restricted Access Mode ensures that data integrity and security 

controls are implemented in accordance with the guidelines of 21 CFR 

Part 11. It assists in compliance with federal Current Good 

Manufacturing Practices (cGMP) by ensuring the integrity of software 

use and electronic files. 

Security Features: 

• User List and Log-in Log 

• Notification of failed Log-In attempts 



21 CFR Part 11: 

Only restricted 

data should be 

imported. 

(global setting) 

• User password change 

• User privileges 

• Session time-out 

• Password expiration 

• Log-in archive 

Electronic record integrity: 

• Limiting access 

• Original record integrity 

• Documentation of changes (by whom and when) 

• Audit trails 

What if I don't work in a 21 CFR compliant environment? 

No problem! 

• It is easy for the software Administrator to assign full access 

privileges to anyone in your organization who will be using the 

software. 

o This will give everyone the ability to use all of the 

system's features, with the exception of the ability to 

access the Administration Options button. 

o All records will contain the Username of the individual 

users 

Other options are: 

• Simply to use the Administrator ID and password for all users, 

thereby giving everyone equal access. 

• Select to run the software in Unrestricted mode. 

First Log-In and Setting up an Administrator 

Please refer to Section 2, page 2 for full instructions on how to set up 

an Administrator and Log-In to the software for the first time. Ideally, 

this will be done soon after the software is installed. 

Administrator Options 

The Administrator (or a user with Administrator access rights) uses the 

Administration Options button, located on the Welcome window, to 

register new users and assign access privileges, as well as manage 

security controls. This button is only available when the software 

Administrator or a user with Administration access is Logged In; in all 

other cases it will not appear on the Welcome window. 


Important! 

• If you test 

Password 

Expiration Period 

or Time Out 

Period features 

using system 

clock changes, the 

software must be 

closed when 

system clock 

changes are 

made. 

• If not the 

Administration 

screen may 

appear with each 

Administrator 

Log-in until 100 

Log-In/Log-Outs 

occur. 

Remember: 

• A user name 

must be at least 1 

character in 

length. 

• The password 

must be at least 

6 characters in 

length, contain 

at least one 

number, and is 

case sensitive. 


Options Tab 

Once you have selected the Administration Options buttons, the 

Administration window will open. By default, it will appear with the 

Options tab selected. Make your selections from the available options 

and then click the Save and Close button. 

• Destination Directory for Log-In-Log files 

Shows the default directory where Log-In Log Archive files are 

saved. Select the desired directory to place in the 

computer/network location of your choosing. You should select a 

secure location (for example, in a secure server or a password 

protected file folder). 

• Password Expiration Period 

Use the pull down menu to select either Three Months or One 

Minute. The default is three months. This requires all users to 

select new passwords after 3 months. Select one minute to expedite 

validation testing only. 

• Time-Out Period 

Use the pull down menu to select either Fifteen Minutes or 10 

seconds. The default is fifteen minutes. Select 10 seconds to 

expedite validation testing only. Please refer to Timed Log Out in 

Section 3, Pg. 5 for more information. 

• Restricted Access Mode 

Click on the checkbox to change the mode (make sure there’s a 

check in the box if you want Restricted Access mode. 



Once the 

administrator 

adds a new 

user name to 

the user list, 

that name can 

never be 

deleted or 

changed. 

Creating a User List 

From the Administration window, select the User List tab. Go to this 

list to add new users and assign levels of software access to those 

users, or to make changes to their level of access. User names can 

never be changed or deleted. 

Adding new users and Assigning Privileges 

1. Click the User List tab. A numbered list will appear, showing all 

users registered to that point, starting with the original Software 

Administrator (in row 1). 

2. To add a new user, click in the next blank field in the Username 

column. Enter the new user name. 

3. Click in the blank Assigned Password field next to that new user 

name. Enter a temporary password for that new user. 

4. Click in each Privilege box to the right, toggling between Yes and 

No to assign or deny specific access levels to that user. 

5. Click the Save and Close button when you are finished. 

6. Give the Username and Password to the person you have 

registered, and refer them to Logging In and Out (starting on Pg. 4, 

Section 3), if they need help logging into and out of the software. 

Remember that the password you have chosen is only temporary; 

the user will be prompted to enter a new password the first time 

they Log-In to the system. 

Each new user must be assigned a level of software access by the 

Administrator. Consult Table 9-1 for a listing of these privileges. 


Log-In Privileges 


Privilege What It Allows What It Does Not Allow 

Log-In User will be able to Log-In and out of 

MicroLog software using a password, and 

will be able to use the Set Up Input and Data 

features (to enter MicroPlate information, 

read MicroPlates, and print results. All Data 

will be saved automatically). 

Set-Up User will be able change screen colors and 

fonts, will be able to access the Detailed 

Reader Setup page (for troubleshooting), and 

can disable the Auto Save feature. 

User cannot use Data Management 

functions 

User cannot use Data Management 

functions 

View/Print User will be able to view or print data files User cannot edit data files 

Edit User will be able to use all Data 

Management features, including editing data 

files and compiling databases. 

Admin User will have complete access to all aspects 

of the software (just like the software 

administrator). 

User cannot perform any 

Administrator functions 

User cannot delete or change user 

names 

TABLE 8-1: USER ACCESS PRIVILEGES, IN DESCENDING ORDER OF LOWEST TO HIGHEST LEVELS OF ACCESS. 

Viewing the Log-In Log 

View the Log-In Log by selecting the Log-In Log tab on the 

Administration window. This feature of the software keeps 

meticulous track of the software's use in descending order of date 

(with the most recent date and log-in first). This log is non-editable. 

The Log-In Log records the past 100 log-in attempts. For each log-in 

attempt, the Log-In Log records the username, date and time loggedin, 

whether the username is registered, and the date and time the 

person logged-out. The log also records which privileges registered 

users have, and shows the software is in Restricted Access Mode. This 

creates an audit trail. As log-in records in excess of 100 drop from the 

list, they are saved to a “Read-Only” file (Logins/LoginLogXXXXX.csv 1 ). 

When this saved Log-In Log archive file contains 100 records, 

subsequent records will be saved to a new Log-In Log archive file with 

a different date stamp 1 . 

1 XXXXX is a five-digit date stamp 



THE LOG-IN LOG 

Table 8-2 defines each of the columns in the Log-In Log and describes 

what they mean. 

Interpreting the Log-In Log 

Column Name Information Given 

Restricted If software was in Restricted Access mode when user logged-in, this entry will 

say Yes; if software was operating in Unrestricted Access mode, this entry will 

say No. 

Entered The user name of each person who logged in is listed here. 

Username 

Registered If this person was an approved user, this entry will say Yes; if not, this entry 

will say No. 

Attempt Date This is the exact date and time the user logged in (or attempted to do so). 

Log-Out Time This is the exact date and time the user logged out. The entry here will read 

Never in the event of a failed log-in and when the software administrator is 

currently using the software. 

User Privileges 

Log-In Yes if access was given during that log-in period; No if not 

Option Yes if access was given during that log-in period; No if not 

View/Print Yes if access was given during that log-in period; No if not 

Edit Yes if access was given during that log-in period; No if not 

Admin Yes if access was given during that log-in period; No if not 

TABLE 8-2: INFORMATION IN THE LOG-IN LOG. 


Remember that the 

password must be at 

least 6 characters in 

length, contain at 

least one number, 

and is case sensitive. 

NOTE: The 

software will 

not allow the 

use of the last 

four passwords 

established for 

a given user 

name. 


Changing or Lost Passwords 

The software requires that all users Log-In with a password to enter 

the software. This ensures a secure environment by controlling access 

to the software. A user can change his or her password at any time. 

Changing your Password 

1. Log-In to the software. 

2. At the Welcome window, select the Change Password button. 

3. The Change Password dialog box appears. 

4. Enter the password that was assigned to you, then enter a password 

chosen by you and reenter it. 

5. Click OK. 

CHANGE PASSWORD DIALOG WINDOW 

Lost or Revoked Password 

Occasionally, a user might simply forget or misplace their password. 

The user may have attempted entry 5 times with the incorrect 

password. If the user is NOT the only Administrator, this situation is 

easily remedied. Any Software Administrator can easily go to the 

User List under Administration and assign the person a new 

password. Make sure to re-assign the user's privileges as well, or the 

new password will not work. 

Logging In 

When the system is operating in Restricted Access Mode, all users 

must Log-In when starting up the software or changing users. 

1. When starting up the software, the software will automatically 

show a Password Dialog window. Enter your user name and 

password, and then click OK. After a few seconds, the Welcome 

window will appear. 



PASSWORD DIALOG WINDOW 

2. If the software is already open and it is necessary to change users, 

go to the Welcome window and click Change Users. A Password 

Dialog window will appear. Enter the new user’s name and 

password. 

Unauthorized log-in attempts 

If someone enters an incorrect user name and/or password, the 

software will allow five attempts to enter the information correctly. 

After five attempts, the software will automatically close. 

Subsequent attempts to open the software and Log-In using an 

incorrect user name or password will result in a warning tone and 

screen message noting that “Unauthorized Access Has Been 

Attempted.” 

The message will remain until the Administrator clicks on the message 

while in the Administration screens. 


9. Technical Notes 


�Materials List 

�Media 

Preparation 

�Specialized 

MicroPlates 

Media 

Inoculating 

Fluid 

Miscellaneous 

Supplies 

MicroPlates 

Technical Notes 

This section gives specific information regarding consumable materials 

you’ll need to perform microbial identifications, as well as instructions for 

how to prepare isolation media and inoculating fluid. 

Materials List 

Item Description 

BUG + B Growth medium for gram-negative and gram-positive bacteria 

BUG + M Growth medium for gram-positive spore-forming rods 

BUG Growth medium for agricultural bacteria 

BUA Growth medium for anaerobes 

BUY Growth medium for yeasts 

CHOC Growth medium for fastidious gram-negative bacteria 

2% ME Growth medium for filamentous fungi (and select yeast species) 

GN/GP-IF Inoculating fluid for gram-negative and gram-positive bacteria 

AN-IF Inoculating fluid for anaerobic bacteria 

FF-IF Inoculating fluid for filamentous fungi (and select yeast species) 

Sterile water Inoculating fluid for yeasts 

Salicylate Inoculating fluid additive for some microbes 

Thioglycolate Inoculating fluid additive for some microbes (available in droppers) 

Streakerz Sterile 6” pointed streaking sticks 

LongSwabs Sterile 7” cotton-tipped swabs 

Transfer pipettes Sterile 6” disposable pipettes, graduated tip, 5 ml 

Reagent reservoirs Sterile reservoirs 

Test tube rack Autoclavable, holds 40 tubes, 20 mm diameter 

Pipette tips Sterile, racked, for repeating multichannel pipettor 

Pipette tips, filtered Sterile, filtered, racked, for repeating multichannel pipettor 

MicroPlate lids Gamma irradiated MicroPlate lids 

GN2 MicroPlates MicroPlates for gram-negative bacteria 

GP2 MicroPlates MicroPlates for gram-positive bacteria 

AN MicroPlates MicroPlates for anaerobic bacteria 

YT MicroPlates MicroPlate for yeasts 

FF MicroPlates MicroPlates for filamentous fungi (and select yeast species) 

TABLE 9-1. CONSUMABLE MATERIALS AND EQUIPMENT 



Caution! 

If you’re making 

your own media, 

follow 

instructions 

exactly. Take 

care to avoid 

contamination. 

Media Preparation 

Biolog can provide you with all the pre-made media you will need for 

microbe identification. However, you can also prepare your own media, 

using Biolog’s formulas. 

Making Biolog Universal Growth Agar + blood (BUG + B) 

1. Mix the following in a 2-3 liter container: 

• 57 grams BUG Agar 

• 950 ml purified, distilled, or deionized water 

2. Gently boil mixture while stirring to dissolve the agar and other 

components. 

3. Cool an aliquot and measure the pH. Adjust pH with NaOH or HCl to 

achieve a final pH of 7.3 + 0.1 at 25° C. 

4. Sterilize by autoclaving at 15 pounds of pressure and 121° C for 15 

minutes. 

5. Cool to 45-50° C. 

6. Add 50 ml sterile fresh defibrinated sheep’s blood just prior to 

dispensing and mix gently. (Use good quality blood with a hematocrit 

of at least 40%). 

7. Dispense into sterile petri dishes. 

Making Biolog Universal Growth Agar + maltose (BUG + M) 

1. Follow steps 1-5 above for BUG + B, except use 990 ml purified water. 

2. Add 10 ml sterile solution of 25% maltose and mix thoroughly. 



Making Biolog Universal Growth Agar (BUG) 

1. Mix the following in a 2-3 liter flask: 

• 57 grams BUG Agar 

• 1,000 ml purified, distilled, or deionized water 


2. Gently boil while stirring to dissolve the agar and other components. 


achieve a final pH of 7.3 + 0.1. 

4. Sterilize by autoclaving at 15 pounds of pressure, at 121° C, for 15 

minutes. 

5. Cool to 45-50° C. 


Making Biolog Universal Anaerobe Agar + blood (BUA + B) 


• 51.7 grams BUA Agar 

• 950 ml purified, distilled, or deionized water 

2. While flushing with oxygen-free nitrogen gas, gently boil mixture 

while stirring to dissolve the agar and other components. 




minutes. Make sure the bottle is tightly capped to prevent the entry of 

oxygen. 

5. Cool to 45-50° C under a stream of oxygen-free nitrogen gas. 

6. Add 50 ml sterile fresh defibrinated sheep’s blood just prior to 

dispensing and mix gently. (Use good quality blood with a hematocrit 

of at least 40%). 

7. Dispense into sterile petri dishes in an anaerobic chamber. 



Making Biolog Universal Yeast Agar (BUY) 


• 60 grams BUY Agar 






minutes. 

5. Cool to 45-50° C. 


Making 2% Malt Extract Agar 


• 20 grams Oxoid Malt Extract (LP0039B) 

• 18 grams Bacteriological Grade Agar 






minutes. 

5. Cool to 45-50° C. 



� 

� 

� 

� 


General hints for culture media preparation 

Keep dehydrated media powder in original bottles with lids tightly closed 

to avoid water absorption and deterioration. 

Use clean dry glassware that has been rinsed free of all soap residue. 

Add water to the vessel first, then weigh agar powder and add it to the 

vessel. Mix to obtain an even suspension. Do NOT fill the vessel more 

than two-thirds full (to avoid boiling over during heating and autoclaving). 

Heat agar gently, with constant stirring. 

Making inoculating fluid (GN/GP-IF) 

Biolog supplies pre-made inoculating fluid, with the following 

composition: 

• 0.40% sodium chloride (NaCl) 

• 0.03% Pluronic F-68 (e.g., Sigma P7061) 

• 0.02% Gellan Gum (e.g., Phytagel, Sigma P8169) 

However, if you wish to prepare your own, using Biolog’s formula. 

1. Add 0.2 grams Gellan Gum to 1 liter of H2O. 

2. Heat to boiling, stirring constantly, until the Gellan Gum is completely 

dissolved. 

3. Turn off the heat but continue stirring. 

4. Add 4 grams NaCl and continue stirring until the NaCl is completely 

dissolved. 

5. Add 0.3 grams of Pluronic F-68. Continue stirring until the Pluronic F- 

68 is completely dissolved. 

6. Dispense the volume appropriate to obtain 19 ml post-autoclaving into 

20 x 150 mm tubes. 


minutes. 

Note: Gellan Gum concentration of 0.01-0.02% is acceptable. 



Making inoculating fluid (FF-IF) 

Biolog supplies pre-made inoculating fluid, with the following 

composition: 

• 0.03% Tween 40 (e.g., Sigma P1504) 

• 0.25% Gellan Gum (e.g., Phytagel, Sigma P8169) 

However, if you wish to prepare your own, using Biolog’s formula. 

1. Heat to boiling 1 liter of H2O. 

2. Add 2.5 grams Gellan Gum and 0.3 grams Tween 40. Stir. 

3. Turn off the heat but continue stirring until all components are 

completely dissolved and the solution becomes clear. 

4. Dispense the volume appropriate to obtain 16 ml post-autoclaving into 

20 x 150 mm tubes. 


minutes. 

Specialized MicroPlates 

Biolog makes a series of additional MicroPlates for special applications. 

While there is currently no database for these, you can use them to create 

your own database with the software. 

Table 9-2 lists these specialized MicroPlates and their uses. Contact 

Biolog or your Biolog Distribution Partner for more information. 

Specialized MicroPlate Use 

SF-N2 and SF-P2 MicroPlates For testing of Sporulating and Filamentous microorganisms (such as 

actinomycetes and fungi) 

MT2 MicroPlate Contains same nutrient base and color chemistry as GN MicroPlate, but 

without carbon sources. Add your own carbon sources to study the 

metabolic capabilities of any microbe of your choice. 

EcoPlate Contains the same color chemistry as the GN2 plate, but has three sets of 31 

the identical carbon sources. 

Note: the A1 well is used as the threshold algorithm control. Running 

different samples on one plate may affect the positive/negative/borderline 

calls. 

TABLE 9-2. BIOLOG SPECIALIZED MICROPLATES 


10. Frequently Asked Questions 


�Answers to 

Common 

Questions 

Q 

A 

Q 

A 

Q 

A 

How I should I store my MicroPlates? 

Frequently Asked Questions 

Unpack MicroPlates as soon as you receive them and keep them 

refrigerated until use. Biolog MicroPlates should be kept refrigerated (not 

frozen). MicroPlates are fairly stable at room temperature; we suggest 

refrigeration because they must be kept cold for maximum shelf life. At 

moderately warm temperatures, they slowly begin to deteriorate. Visually 

examine MicroPlates before using them. You may see faint yellow-brown 

or pink shades in wells when they are dry (this is OK). However, if wells 

show significant color immediately after inoculating the MicroPlate with 

the cell suspension, they have most likely been heat damaged. 

Do I have to handle frozen or lyophilized cultures in a special way? 

Yes. Subculture frozen or lyophilized cultures two to three times before 

testing. 

Our lab would really like to use our own media. We know that our strains 

will grow on it. Is that all right? 

No. However tempting it may be to use nonrecommended media, using 

anything other than Biolog-recommended media is a mistake. The carbon 

source tests in our MicroPlates are configured for precise metabolic 

reactions. The metabolic state of an organism has a profound influence on 

the resulting MicroPlate pattern that is the basis for identification. Many 

species will give fewer positive reactions when grown on nonrecommended 

media. Use Biolog-recommended media only! 



Q 

A 

Q 

A 

Q 

A 

Q 

A 

Why is there a defined range for the turbidity of inocula? 

It is essential to prepare inocula in a consistent and precise manner. The 

Biolog redox test chemistry is sensitive to oxygen concentration, which is 

determined by cell density. Use Biolog turbidity standards and calibrate 

your turbidimeter. 

Are Biolog’s Turbidity standards comparable to McFarland standards? 

Biolog’s Turbidity standards have different set points and are not 

comparable to McFarland standards. Biolog’s standards are set for cell 

densities at which the Biolog test performs optimally. Use of Biolog 

standards is necessary to achieve consistent and accurate results. 

Can I use one incubator for all my strains and set it at 32°-33°c instead of 

having two incubators at 30° c and 35°-37° c respectively? 

Organisms grow better depending on the temperature of their 

environment. Biolog has chosen the temperatures at which the organisms 

in out database grow optimally. Using different temperatures than those 

recommended will decrease the performance of your Biolog ID system. 

We read our MicroPlates visually. Occasionally we are unsure whether a 

reaction is positive or negative. How should we enter those wells? 

Light purple reactions are considered positive as long as the color is 

noticeable when compared to the A1 reference well. However, if you’re 

still unsure, enter these reactions as “borderline.” If, for example, you 

enter 4 out of 95 tests as borderline, the software will ignore the 4 

borderline tests and base its identification on the remaining 91 tests. This 

will give you far more accurate results than guessing wrong about 

reactions you’re not sure of. 


Q 

A 

Q 

A 

Q 

A 


What should I do if I get different ID results at 4 hours and at 24 hours? 

This is most often the result of having a mixed culture. See Section 4 for 

recommended procedures and examine your culture carefully to see if it is 

mixed. Also, consider the similarity value at one incubation time vs. the 

other. Environmental strains will often grow a little differently than the 

lab strains used to make the databases. Some strains may have a high 

similarity in our 4 hour database, while others match our 24 hour database 

more closely. Compare you similarity values to see which is a better 

match. 

Are there any genera where the taxonomy is still undergoing change? 

Taxonomists have not yet agreed upon how all species should be 

delineated and microbiology is an ever-evolving science. We update and 

expand our software library on a continual basis, but at this point there are 

certain genera still undergoing revision. These include Bacillus, 

Corynebacterium, Enterobacter, Pseudomonas, Aeromonas, Vibrio, 

Acinetobacter, Moraxella, Clostridium, and Candida. 

www.dsmz.de/bactnom is a good site for current bacterial nomenclature. 

You can also link to this site through Biolog’s web page. 

www.cbs.knaw.nl is a good site for current yeast and filamentous fungi 

nomenclature and information. 

Our lab is interested in your quality control and validation procedures. 

Where do we get this information? 

Material Safety Data Sheets, Quality Control organism information, and 

Certificates of Performance are available on the website. Validation 

procedures, reagents kits and bacterial strains are available from Biolog. 


11. Troubleshooting 


�Gram Stain 

Identification 

�Culturing 

Microorganisms 

�Preparing Inocula 

�Inoculating 

MicroPlates 

�Incubating 

MicroPlates 

�MicroStation 

Reader 

Troubleshooting 

Carefully following the instructions in this guide will greatly minimize 

problems. Occasionally, however, you may get stuck or encounter 

difficulties. This section addresses the symptoms, causes, and solutions to 

those occasional problems. If you still can’t figure out the cause of the 

problem, call Biolog Technical Service. We’re always glad to help. 

Additional help can be found on Biolog’s website at www.biolog.com. 

Gram Stain Identification 

Since Gram stain readings start the chain of steps leading to a 

MicroStation/ MicroLog identification, it’s essential to perform them 

according to standard lab protocol and to interpret results correctly. 

� 

� 

� 

� 

View the smear with a light microscope, using the oil-immersion 

objective. Gram-positive bacteria appear blue or violet; gram-negative 

bacteria appear pink or red. 

Gram-negative bacteria may appear gram-positive if the smear is too thick 

and decolorization is incomplete. 

Gram-positive bacteria may appear gram-negative if the smear is overdecolorized. 

This can also occur if the culture is not fresh and has reached 

stationary phase (some Bacillus species are gram positive for only a few 

divisions after spore germination). In addition, gram-positive bacteria will 

appear gram-negative if the integrity of their cell walls is damaged. 

To prevent misidentification, prepare light smears of young, actively 

growing cultures. Use known gram-positive and gram-negative controls. 

When you’re determining organism morphology, perform Gram stains 

from liquid medium. Solid agar can affect the appearance of the organism. 



Symptom Cause Solution 

Gram variability Smear is too thick Make a single-cell-layer smear. 

Run positive and negative controls. 

All smears appear Overwarming of slides Air dry slides completely before warming. 

gram negative 

Don’t use a flame to fix slides. 

Using old cultures Prepare slides from fresh log-phase growth cultures. 

Over-decolorizing Repeat Gram stain, using specified timing. 


Morphology unclear Growth on agar media 

affects morphological 

appearance 

Do a Gram stain from broth culture. 

Too-intense color Under-decolorizing Use decolorizer made by same company that makes 

your stains. 

Prepare decolorizer or acetone/alcohol at several 

concentrations. Test solutions at 5, 10, and 15 seconds 

with known positive, negative, and weakly positive 

cultures. 


Additional differentiation techniques 

Make sure you’re using proper Gram stain procedure. If you still get 

ambiguous results, use the following table as a guide: 

Test Reactions 

Vancomycin sensitivity (30 µg disks) Gram-negative bacteria � resistant 

Gram-positive bacteria � most are sensitive 

Growth on MacConkey and CNA plates Gram-negative bacteria � MacConkey +, CNA – 

Gram-positive bacteria � MacConkey –, CNA + 

KOH test (3%)* Gram-negative bacteria � suspension becomes thick 

and stringy 

L-alanine aminopeptidase activity (use 

Gram-negative bacteria � + 

commercially available test strips impregnated 

with a colorimetric substrate for this enzyme)* 

Gram-positive bacteria � – 

*For test procedures, see Bailey & Scott’s Diagnostic Microbiology, Baron E.J. and Finegold, S.M., C.V. Mosby 

Co., 1990, pages 102-103. 


Culturing Microorganisms 


Poor overall growth Using nonrecommended 

media 

Use Biolog-recommended media. 

Isolate takes several 

days to form a colony 

Bacterium will not 

grow on BUG + B or 

forms pinpoint 

colonies 

Slow growth and/or 

weak pattern 

formation 

Mixed growth on 

agar plates 

Some environmental 

organisms take several 

days to become visible on 

growth plate, at which 

point culture may be too 

old for successful ID 

Fastidious gram-negative 

bacteria need special 

culture conditions 

Some environmental 

bacteria may be 

oligotrophic or temporarily 

in an oligotrophic state 

(i.e., they will only grow 

on low nutrient media such 

as R2A) 

Sub-optimal growth 

temperature and/or 

humidity and/or 

atmosphere 

Sticky bacterial surfaces 

Filamentous fungi mixed 

with bacteria 


Subculture one or two passages in broth medium. 

Set up two or three agar plates to obtain sufficient 

growth. 

Use Chocolate agar and incubate with elevated CO2 at 

35-37° C. 

Try to subculture the bacterium from R2A and see if 

they will gradually adapt to growing on BUG + B. If 

not, it is a species that is not included in our 

MicroStation/MicroLog database. 

Agricultural bacteria may be grown on BUG without 

blood. 

Use specified incubating temperatures (see Section 4 

and Appendices). 

If your unknown organism came from a cold or warm 

environment, grow it first at its environmental 

temperature, then try to grow it at 35-37° C, 30° C, or 

26° C. 

Add a pan of water to provide humidity in your 

incubator. 

Incubate the MicroPlate at the same temperature as 

the growth plate. 

Transfer a colony into a few ml of GN/GP-If or sterile 

water, vortex for several seconds, and streak out from 

the cell suspension onto a medium that aids the 

detection of subtle differences in colony morphology. 

Take a single colony and streak for isolation on a 

separate plate. Repeat as necessary until you have a 

pure culture. 

Cultures can be streaked on media containing 

antibacterial antibiotics. If this fails, use 1 drop of 

saline suspension of the mixed culture to inoculate 

each of four tubes of Sabouraud broth (1.0 ml). Add 

in series either 1,2,3 or 4 drops of concentrated HCl. 

Streak out from each tube. 



Preparing Inocula 


Incorrect 

turbidity 

Inaccurate %T 

measurements 

Trouble making a 

homogenous 

suspension 

Too few positive 

reactions in 

MicroPlates 

Turbidimeter out of 

calibration 

Tubes not properly 

blanked 

Significant scratches 

or smudges on tube 

Nonuniform 

suspension of cells 

Mucoid or clumpy 

cells 

Detergent traces in 

tube 

Calibrate accurately. 

Use correct standards. 

Make sure standards have not expired. 

Once you blank a tube, do not rotate it while making 

suspension. 

Blank each suspension tube (tubes are not optically precise and 

can vary tube-to-tube and with rotation). 

Use suspension tubes only once, then discard. 

Visually inspect tubes before using. 

Wipe outside of tubes with a tissue before placing in 

turbidimeter. 

Use 7” swab to mix cells all the way to the bottom of the tube. 

The light path that looks through the tube is only viewing the 

bottom of the tube. 

For Spore Forming Gram positive Rods, Follow the 

recommendations on pages 4-10 to 4-12. 

For Non-Spore formers: 

1. Roll swab over young colonies in 3 rd and 4 th quadrants. 

2. Mash colonies against side of a sterile tube (above meniscus). 

3. Add several ml of inoculating fluid and wash the sides of the 

tube with a cotton swab. 

4. Mix well, then examine for clumps. If clumps are present, 

allow them to settle. 

5. Transfer this concentrated inoculum into a fresh Inoculating 

Fluid tube until the recommended turbidity is reached. 

* Be careful not to transfer any colony clumps into the 

Inoculating fluid. 

1. For difficult organisms such as Gornia or Tsukamurella, 

suspend colonies as a dense suspension in a small amount of 

inoculating fluid as above. 

2. Incubate as needed at 35° C (do not exceed 10 minutes). 

Vortex (if clumps still present, pull off supernatant and 

repeat). 

3. Bring supernatant volume up to 18 ml and mix until 

homogenous. 

Use suspension tubes only once, then discard. 

Use of “bad” water Use high quality purified water without preservatives. 


Inoculating MicroPlates 



Pipetting problems Wells inoculated unevenly Dispense first aliquot from pipettor back into reservoir. 

Depress lever smoothly and completely. 

Make sure tips are lined up evenly and tightly seated. 

Avoid pipetting or trapping air bubbles. 

Visually check wells after inoculating. 

Incorrect volumes used 

Incubating MicroPlates 

100 µl for anaerobic bacteria, yeasts and fungal. 

150 µl for aerobic bacteria. 


Poor growth or poor 

pattern formation in 

MicroPlates 

Wells at the four 

corners or edges of 

the MicroPlate have 

lower liquid levels 

after incubation 

Wrong incubation 

conditions 

Wrong MicroPlate 

Organism not in Database 

Incubator is too dry 

Filamentous fungi not 

incubated in a closed 

container 

Incubate according to specified temperature and 

conditions. See Section 4 and Appendices. 

Incubate the MicroPlate at the same temperature as 

the growth plate. 

Make sure incubator humidity is sufficient. 

Add a pan of water to humidify incubator, or incubate 

MicroPlates in a plastic container humidified with 

moist paper towels. 

Make sure you have the FF MicroPlates in a closed 

container or in plastic bags on trays. Do not add 

additional moisture when incubating filamentous 

fungi. 



MicroStation Reader 


Erratic or inaccurate 

reading 

Software won’t 

communicate with or 

initialize reader 

MicroPlate won’t go 

into reader 

Moisture, scratches, or 

smudges on MicroPlate 

Many borderline reactions 

on MicroPlate 

Remove MicroPlate lid before reading. 

Wipe bottom of MicroPlate before putting into reader. 

Review all sample preparation procedures for 

correctness and accuracy. 

Reading artifacts Always double-check results by eye. 

Verify that your visual reading coincides with reader 

results. 

Use the Histogram software function to manually 

adjust threshold if necessary. 

Wrong com port selected Choose correct com port. 

Loose cable connection Turn reader off. Unplug cable, then plug it back in. 

Turn reader on and try again. 

Unknown cause Turn reader off and exit program. Restart program, 

turn reader on, and try again. 

The reader has its own error messages, which should 

be self-explanatory. Call Biolog Technical Service if 

you need further assistance. 

Reader Bulb is Out/Low Use the System Check test in the Bio-Tek reader 

manual. (Use Air reference test in the Molecular 

Devices reader manual.) 

MicroPlate mispositioned Make sure MicroPlate snaps into place and is seated 

levelly. 

Make sure MicroPlate lid is off before reading. 

Remove MicroPlate and reposition in reader tray. 

Make sure A1 well is at the top left. 

Verify that software is communicating with reader. 


12. Glossary 


� Definitions of 

Terms 

Anamorph 

Ascospore 

Ascus 

Asexual state for yeasts and filamentous fungi. 

Glossary 

Sexual spore formed within an ascus in fungi of the division Ascomycota. There 

are usually 4 or 8 ascospores per ascus (sometimes 2 or multiples of 4). 

Cell in the shape of a sac (round, club-shaped or cylindrical) within which 

ascospores are produced. 

Aseptic technique 

Standard lab procedures used to prevent contamination. 

Basidiospore 

Basidium 

A sexual spore formed on the upper surface of a basidium in fungi of the division 

Basidiomycota. There are usually 4 basidiospores, sometimes 2, and rarely 

multiples of 4. 

A club shaped cell upon which basidiospores form. 

Carbon source utilization 

Basic process used to identify microbes based on the chemicals they can utilize. 

Catalase test 

Additional test used to characterize gram-positive bacteria. 

Cluster analysis 

Mathematical depiction of the relationship between closely related microbe 

identification patterns. Shows dendrograms and two- and three-dimensional 

diagrams. 

Conidium 

A unicellular or multicellular fungal element specialized to detach from the 

mycelium and disseminate, thus serving as an asexual reproductive structure. 


Glossary 

Dendrogram 

Cluster diagram in the form of a branching tree. 

Endpoint ID (EID) 

Biolog, Inc. developed pattern matching method which considers the daily 

endpoint determination. 

Enteric 

Freeze 

Gram-negative bacteria belonging to the group Enterobacteriaceae. 

The act of converting a database into a Read Only and non-editable format. 

Gram negative 

Bacteria showing typical pink or red reaction on Gram stains. 

Gram positive 

Bacteria show typical blue or violet reaction on Gram stains. 

Histogram 

Hypha 

Inocula 

Visual representation of MicroPlate color distribution and thresholds. 

Septate or aseptate filaments of a fungus. 

Cell suspension used to inoculate MicroPlates. 

Inoculating fluid 

Fluid used to prepare inocula. 

Maltose 

A sugar added to BUG Agar. Required for culturing spore-forming gram-positive 

rods (e.g., Bacillus species). 

Manual mode 

Software mode used to record MicroPlate reactions visually. 

MicroStation/MicroLog software 

Windows-based program for microbe identification. 

MicroPlate 

Plate with 95 carbon source utilization tests (one in each well), with A1 well as 

control. The MicroPlate for yeasts has 94 tests and two control wells (A1 and D1). 


MicroPlate reactions 

Positive, negative, and borderline color responses used to identify microbes. 

Glossary 

MicroStation System 

Comprehensive system for microbe identification, including computer, 

MicroStation/MicroLog software, MicroStation reader, pipettor, turbidimeter, and 

ancillary materials. 

Non-enteric 

Oxidase test 

Pattern 

Pleomorphic 

Gram-negative bacteria not belonging to the group Enterobacteriaceae. 

Additional test used to characterize gram-negative bacteria. 

Color responses in MicroPlates. 

Having various distinct forms or shapes exhibited by a single strain or species 

Progressive ID (PID) 

Biolog, Inc. developed pattern matching method which considers the progressive 

sequence in which purple wells are formed. 

Pure culture 

Culture containing only one microbe species. 

Reader mode 

Software mode used to register MicroPlate reactions from MicroStation Reader. 

Read Only File 

The MicroStation/MicroLog Program will prevent editing of designated Read 

Only files 

Restricted Access mode 

Mode set by system administrator to prevent unregistered users from using 

system; assign user names, passwords and privileges to each user; create an audit 

trail, and freeze data files to maintain data integrity. 

Salicylate 

Anticapsulate agent required as an addition to inoculating fluid for a few grampositive 

species. 

Sporangiospore 

An asexual spore produced by cytoplasmic cleavage within a sporangium. 


Glossary 

Spore, fungal 

Sterile, fungi 

Teleomorph 

Thioglycolate 

Thresholds 

TSI slant 

Turbidity 

A fungal propagative element, produced either as part of a sexual process 

(ascospore, basidiospore, zygospore), or by an asexual reproductive process 

involving a process of cytoplasmic cleavage (sporangiospore). 

Used to describe a fungal culture which produces no spores or conidia. 

Sexual state for yeasts and filamentous fungi. 

Anticapsulate agent required as an addition to inoculating fluid for gram-negative 

enteric bacteria, gram-negative fastidious bacteria, and gram-positive cocci and 

rods. 

The optical boundaries between negative, borderline, and positive reactions. 

Additional test used to characterize gram-negative bacteria. 

Measurement of cloudiness, which is indicative of inocula cell densities. 

Unrestricted Access mode 

Mode set by system administrator to allow any user to use all software functions. 

Zygospore 

A sexual spore formed from the fusion of two similar cells called gametangia; this 

spore is characteristic of fungi in the division Zygomycota. 


13. Appendices 


�Plate Data 

Statistics 

� Setup 

Flowcharts 

� Database 

Species Lists and 

Their 

Characteristics 

� Program 

Printouts 

Plate Type 

GN, GP, 

MT or 

Eco: 

AN: 

YT or 

Other: 

FF: 

General Read Statistics 

-Dual wavelength color statistic (590, 750) 

-A1-zeroed OD 750 turbidity statistic 

-Progressive identification algorithm 

-0.75, 0.5 similarity cut-offs for 4-6 hour, and 24 

hours 



-Progressive identification algorithm 

-0.5 similarity cut-offs for 20-24 hours 

-Single wavelength color statistic (590) 

-End Point identification algorithm 

-0.75, 0.5, 0.5 similarity cut-offs for 24 hours, 48 

hours, and 72 hours 



-End-point identification algorithm 

-0.9, 0.7, 0.65, 0.6, 0.6 similarity cut-offs for 

1,2,3,4, and 7 days (7 day reading is present, 

however not included in the identification 

procedure) 

Appendix 1: Plate Data Statistics 

Appendix 1: Data Statistics 

For Various Plate Types 

Minimum Number of 

positives to call an ID 

4-6 hour 3 

read 

24 hour 3 

read 

20-24 hour 7 

read 

24 hour 3 

read 

48 hour 3 

read 

72 hour 3 

read 

24 hour 30 

48 hour 25 

72 hour 20 

96 hour 15 

168 hour 5 

ID specific limitations: 

Maximum number of 

borderline Reactions 

25 Borderline 

Reactions 

25 Borderline 

Reactions 

25 Borderline 

Reactions 

50 Borderline 

Reactions 


Culture Media 

Atmosphere 

Temperature 

Inoculating Fluid 

Inoculum %T 

MicroPlate - µl 

per well 

Incubation Time 

(hours) 

Appendix 2: Release 4.2 Setup Flowchart 

Oxi + 

Appendix 2: Release 4.2 Setup Flowchart (non-FF) 

Oxi – 

GN 

GN-NENT GN-ENT GN-FASb BUG+B 

BUG+B 

CHOC 

AIR 

Typically 

30°C 

GN/GP-IF f 

52%T 

GN2 – 150 µl 

4-6, 16-24 

30°C 

TSI=K/K 

or K/A w 

TSI=A/A 

or K/A 

AIR 

Typically 

35°-37°C 

GN/GP-IF+T 

61%T 

GN2 – 150 µl 

4-6, 16-24 

Determine Gram Stain Morphology 

and Optimal Incubation Temperature a 

and Atmosphere 

If no 

reactions 

Aerobic Bacteria 

6.5 % CO 2 

Typically 

35°-37°C 

GN/GP-IF+T 

20%T 

GN2 – 150 µl 

4-6, 16-24 

MicroStation System/MicroLog User Guide Apr 07 Section 13 �Page 2 

GP 

GP-COCCUS GP-ROD 

BUG+B c 

AIR/6.5 % CO 2 d 

Typically 

35°-37°C 

GN/GP-IF+T g 

20%T 

GP2 – 150 µl 

4-6, 16-24 

GP-ROD SB 

BUG+M+T 

(See 4.9-13 PLUS 

streaking) 

AIR 

Typically 

30°C 

GN/GP-IF 

28%T 

GP2 – 150 µl 

4-6, 16-24 

Yeast 


AN 

BUA+B 

a 

With the exception of thermophiles, all species are either 26°C, 30° or 35°-37°C. 

f 

If control well (A1) is positive, add thioglycolate to inoculating fluid. 

b 

Requiring CHOC or CO2 for growth or forming

Appendix 3: FF Setup Flowchart 

Appendix 3: FF Setup Flowchart 

Sample Preparation Process for FF MicroPlate 

Initial culture medium 2% ME 

Wet prep results Hyphal elements Yeast cells 

Microbe type Filamentous Fungi Yeast 

Culture medium 2% ME 2% ME 

Temperature 26° C 26° C 

Atmosphere Air Air 

Culture Incubation time 5 - 10 days 2 days 

Inoculating fluid 

FF-IF FF-IF 

Inoculum turbidity/ 

Turbidity standard 

MicroPlate type/ 

µl per well 

Incubation time 

(hours) 

75% T 

75% T 

FF 

FF 

FF 

FF 

100 

100 

24, 48, 72, 96 24, 48, 72, 96 

*Note: We recommend that filamentous fungi be manipulated in a biological safety cabinet. 

ABBREVIATIONS 

2% ME = 2% Malt Extract Agar 

FF = Filamentous Fungi 

%T = percent transmittance 

IF = inoculating fluid 


Appendices: Notes 

Notes: 

Section 13 � Page 4 - MicroStation System/MicroLog User Guide Apr 07

†Agricultural bacteria that may be grown on BUG without blood. 

** Found in the Dangerous Pathogen database 

Appendix 4: Database Species Lists and Their Characteristics 

Appendix 4: Database Species Lists and Their 

Characteristics 

Gram-Negative Aerobic Bacteria 

Species Name Type Test Thio Medium Atm Temp 

1 "Achromobacter cholinophagum" GN-NENT O+ N BUG+B Air 30 

2 Achromobacter xylosoxidans ss denitrificans GN-NENT O+ N BUG+B Air 30 

3 Achromobacter xylosoxydans ss xylosoxidans GN-NENT O+ N BUG+B Air 30 

4 Acidovorax avenae ss avenae GN-NENT O+ N BUG+B† Air 30 

5 Acidovorax avenae ss cattleyae GN-NENT O+ N BUG+B† Air 30 

6 Acidovorax avenae ss citruli GN-NENT O+ N BUG+B† Air 30 

7 Acidovorax delafieldii GN-NENT O+ N BUG+B† Air 30 

8 Acidovorax facilis GN-NENT O+ N BUG+B† Air 30 

9 Acidovorax konjaci GN-NENT O+ N BUG+B† Air 30 

10 Acidovorax temperans GN-NENT O+ N BUG+B† Air 30 

11 Acinetobacter baumanii/genospecies 2 GN-NENT O- N BUG+B Air 30 

12 Acinetobacter calcoaceticus bv alc GN-NENT O- N BUG+B Air 30 

13 Acinetobacter calcoaceticus/genospecies 1 GN-NENT O- N BUG+B Air 30 



16 Acinetobacter genospecies 6 GN-NENT O- N BUG+B Air 30 





21 Acinetobacter haemolyticus/genospecies 4 GN-NENT O- N BUG+B Air 30 

22 Acinetobacter johnsonii/genospecies 7 GN-NENT O- N BUG+B Air 30 

23 Acinetobacter junii/genospecies 5 GN-NENT O- N BUG+B Air 30 

24 Acinetobacter lwoffii/genospecies 8/9 GN-NENT O- N BUG+B Air 30 

25 Acinetobacter radioresistens/genospecies 12 GN-NENT O- N BUG+B Air 30 

26 Actinobacillus capsulatus GN-FAS O+ Y CHOC 6.5%CO2 35-37 

27 Actinobacillus equuli GN-FAS O+/- Y CHOC 6.5%CO2 35-37 

28 Actinobacillus hominis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

29 Actinobacillus indolicus GN-FAS O+ Y CHOC 6.5%CO2 35-37 

30 Actinobacillus lignieresii GN-FAS O+/- Y CHOC 6.5%CO2 35-37 

31 Actinobacillus minor GN-FAS O+ Y CHOC 6.5%CO2 35-37 

32 Actinobacillus muris GN-FAS O+/- Y CHOC 6.5%CO2 35-37 

33 Actinobacillus pleuropneumoniae GN-FAS O+/- Y CHOC 6.5%CO2 35-37 

34 Actinobacillus porcinus GN-FAS O+ Y CHOC 6.5%CO2 35-37 

35 Actinobacillus rossii GN-FAS O+ Y CHOC 6.5%CO2 35-37 

36 Actinobacillus salpingitidis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

37 Actinobacillus seminis GN-FAS O+/- Y CHOC 6.5%CO2 35-37 

38 Actinobacillus suis GN-FAS O+/- Y CHOC 6.5%CO2 35-37 

39 Actinobacillus ureae GN-FAS O+/- Y CHOC 6.5%CO2 35-37 

40 Aeromonas allosaccharophila GN-NENT O+ N BUG+B Air 30 

41 Aeromonas caviae DNA group 4 GN-NENT O+ N BUG+B Air 30 

42 Aeromonas encheleia GN-NENT O+ N BUG+B Air 30 

43 Aeromonas enteropelogenes GN-NENT O+ N BUG+B Air 30 

44 Aeromonas eucrenophila DNA group 6 GN-NENT O+ N BUG+B Air 30 

45 Aeromonas hydrophila DNA group 1 GN-NENT O+ N BUG+B Air 30 

46 Aeromonas hydrophila-like DNA group 2 GN-NENT O+ N BUG+B Air 30 

47 Aeromonas hydrophila-like DNA group 3 GN-NENT O+ N BUG+B Air 30 

48 Aeromonas ichthiosmia GN-NENT O+ N BUG+B Air 30 

49 Aeromonas jandaei DNA group 9 GN-NENT O+ N BUG+B Air 30 

50 Aeromonas media DNA group 5b GN-NENT O+ N BUG+B Air 30 

51 Aeromonas media-like DNA group 5a GN-NENT O+ N BUG+B Air 30 

52 Aeromonas salmonicida ss achromogenes GN-NENT O+ N BUG+B Air 30 

53 Aeromonas salmonicida ss masoucida GN-NENT O+ N BUG+B Air 30 

54 Aeromonas salmonicida ss salmonicida GN-NENT O+ N BUG+B Air 30 

55 Aeromonas schubertii DNA group 12 GN-NENT O+ N BUG+B Air 30 

56 Aeromonas sobria DNA group 7 GN-NENT O+ N BUG+B Air 30 

57 Aeromonas trota DNA group 13 GN-NENT O+ N BUG+B Air 30 




58 Aeromonas veronii DNA group 10 GN-NENT O+ N BUG+B Air 30 

59 Aeromonas veronii/sobria DNA group 8 GN-NENT O+ N BUG+B Air 30 

60 Alcaligenes faecalis ss faecalis GN-NENT O+ N BUG+B Air 30 

61 Alysiella filiformis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

62 Aminobacter aminovorans GN-NENT O+ N BUG+B Air 30 

63 Ancylobacter aquaticus GN-NENT O+ N BUG+B Air 30 

64 Aquaspirillum autotrophicum GN-NENT O+ N BUG+B Air 30 

65 Aquaspirillum dispar GN-NENT O+ N BUG+B Air 30 

66 Aquaspirillum metamorphum GN-NENT O+ N BUG+B Air 30 

67 Aquaspirillum peregrinum ss integrum GN-NENT O+ N BUG+B Air 30 

68 Aquaspirillum peregrinum ss peregrinum GN-NENT O+ N BUG+B Air 30 

69 Aquaspirillum putridiconchylium GN-NENT O+ N BUG+B Air 30 

70 Bergeyella zoohelcum GN-NENT O+ N BUG+B Air 30 

71 Bordetella avium GN-NENT O+ N BUG+B Air 35-37 

72 Bordetella bronchiseptica GN-NENT O+ N BUG+B Air 35-37 

73 Bordetella hinzii GN-NENT O+ N BUG+B Air 30 

74 Bordetella holmesii GN-NENT O- N BUG+B Air 30 

75 Bordetella parapertussis GN-NENT O- N BUG+B Air 35-37 

76 Bordetella pertussis GN-NENT O+ N BUG+B Air 35-37 

77 Bordetella trematum GN-NENT O- N BUG+B Air 30 

78 Bordetella-like species GN-NENT O+ N BUG+B Air 35-37 

79 Brenneria rubrifaciens GN-ENT O- N BUG+B† Air 30 

80 Brenneria salicis GN-ENT O- N BUG+B† Air 30 

81 Brevundimonas diminuta GN-NENT O+ N BUG+B Air 30 

82 Brevundimonas vesicularis GN-NENT O+/- N BUG+B Air 30 

83 Brucella melitensis (abortus) GN-FAS O+ N CHOC** 6.5%CO2 35-37 

84 Brucella melitensis (abortus, melitensis) GN-FAS O+ N CHOC** 6.5%CO2 35-37 

85 Brucella melitensis (melitensis) GN-FAS O+ N CHOC** 6.5%CO2 35-37 

86 Brucella melitensis (suis, canis) GN-FAS O+ N CHOC** 6.5%CO2 35-37 

87 Brucella melitensis (suis) GN-FAS O+ N CHOC** 6.5%CO2 35-37 

88 Budvicia aquatica GN-ENT O- Y BUG+B Air 35-37 

89 Burkholderia andropogonis GN-NENT O+/- N BUG+B† Air 30 

90 Burkholderia caryophylli GN-NENT O+ N BUG+B† Air 30 

91 Burkholderia cepacia GN-NENT O+/- N BUG+B† Air 30 

92 Burkholderia gladioli GN-NENT O+/- N BUG+B† Air 30 

93 Burkholderia glathei GN-NENT O+ N BUG+B† Air 30 

94 Burkholderia glumae GN-NENT O+ N BUG+B† Air 30 

95 Burkholderia mallei GN-NENT O+/- N BUG+B†** Air 30 

96 Burkholderia multivorans GN-NENT O+ N BUG+B† Air 30 

97 Burkholderia phenazinium GN-NENT O+ N BUG+B† Air 30 

98 Burkholderia plantarii GN-NENT O+ N BUG+B† Air 30 

99 Burkholderia pseudomallei GN-NENT O+ N BUG+B†** Air 30 

100 Burkholderia pyrrocinia GN-NENT O- N BUG+B† Air 30 

101 Burkholderia vietnamiensis GN-NENT O+ N BUG+B† Air 30 

102 Buttiauxella agrestis GN-ENT O- Y BUG+B Air 35-37 

103 Buttiauxella brennerae GN-ENT O- Y BUG+B Air 35-37 

104 Buttiauxella ferragutiae GN-ENT O- Y BUG+B Air 35-37 

105 Buttiauxella gaviniae GN-ENT O- Y BUG+B Air 35-37 

106 Buttiauxella izardii GN-ENT O- Y BUG+B Air 35-37 

107 Buttiauxella noackiae GN-ENT O- Y BUG+B Air 35-37 

108 Buttiauxella warmboldiae GN-ENT O- Y BUG+B Air 35-37 

109 CDC group DF-3 (Capnocytophaga) GN-FAS O- Y CHOC 6.5%CO2 35-37 

110 CDC group EF-4 (Neisseria) GN-FAS O+ Y CHOC 6.5%CO2 35-37 

111 CDC group EO-2 GN-NENT O+ N BUG+B Air 30 

112 CDC group II-E subgroup A GN-NENT O+ N BUG+B Air 30 

113 CDC group II-E subgroup B GN-NENT O+ N BUG+B Air 30 

114 CDC group II-H GN-NENT O+ N BUG+B Air 30 

115 Capnocytophaga canimorsus GN-FAS O+ Y CHOC 6.5%CO2 35-37 

116 Capnocytophaga cynodegmi GN-FAS O+ Y CHOC 6.5%CO2 35-37 

117 Capnocytophaga gingivalis GN-FAS O- Y CHOC 6.5%CO2 35-37 

118 Capnocytophaga granulosa GN-FAS O- Y CHOC 6.5%CO2 35-37 

119 Capnocytophaga haemolytica GN-FAS O- Y CHOC 6.5%CO2 35-37 

120 Capnocytophaga ochracea/sputigena GN-FAS O- Y CHOC 6.5%CO2 35-37 

121 Cardiobacterium hominis GN-NENT O+ N BUG+B Air 35-37 

122 Cedecea davisae GN-ENT O- Y BUG+B Air 35-37 

123 Cedecea lapagei GN-ENT O- Y BUG+B Air 35-37 

124 Cedecea neteri GN-ENT O- Y BUG+B Air 35-37 

125 Chromobacterium violaceum GN-NENT O+/- N BUG+B Air 30 








126 Chryseobacterium balustinum GN-NENT O+ Y BUG+B Air 30 

127 Chryseobacterium gleum /indologenes GN-NENT O+ Y BUG+B Air 30 

128 Chryseobacterium indoltheticum GN-NENT O+ Y BUG+B Air 30 

129 Chryseobacterium meningosepticum GN-NENT O+ N BUG+B Air 30 

130 Chryseobacterium scophthalmum GN-NENT O+ N BUG+B Air 30 

131 Chryseomonas luteola GN-NENT O- N BUG+B Air 30 

132 Citrobacter amalonaticus GN-ENT O- Y BUG+B Air 35-37 

133 Citrobacter braakii GN-ENT O- Y BUG+B Air 35-37 

134 Citrobacter farmeri GN-ENT O- Y BUG+B Air 35-37 

135 Citrobacter freundii GN-ENT O- Y BUG+B Air 35-37 

136 Citrobacter gillenii GN-ENT O- Y BUG+B Air 35-37 

137 Citrobacter koseri GN-ENT O- Y BUG+B Air 35-37 

138 Citrobacter murliniae GN-ENT O- Y BUG+B Air 35-37 

139 Citrobacter rodentium GN-ENT O- Y BUG+B Air 35-37 

140 Citrobacter sedlakii GN-ENT O- Y BUG+B Air 35-37 

141 Citrobacter werkmanii GN-ENT O- Y BUG+B Air 35-37 

142 Citrobacter youngae GN-ENT O- Y BUG+B Air 35-37 

143 Comamonas terrigena GN-NENT O+ N BUG+B† Air 30 

144 Comamonas testosteroni GN-NENT O+ N BUG+B† Air 30 

145 Cytophaga fermentans GN-NENT O- N BUG+B Air 30 

146 Delftia acidovorans GN-NENT O+ N BUG+B† Air 30 

147 Edwardsiella hoshinae GN-ENT O- Y BUG+B Air 35-37 

148 Edwardsiella ictaluri GN-ENT O- Y BUG+B Air 30 

149 Edwardsiella tarda GN-ENT O- Y BUG+B Air 35-37 

150 Eikenella corrodens GN-FAS O+ Y CHOC 6.5%CO2 35-37 

151 Empedobacter brevis GN-NENT O+ N BUG+B Air 30 

152 Enterobacter aerogenes (Klebsiella mobilis) GN-ENT O- Y BUG+B Air 35-37 

153 "Enterobacter agglomerans" bgp 2 GN-ENT O- Y BUG+B Air 35-37 


155 "Enterobacter agglomerans" bgp 4 (Pantoea) GN-ENT O- Y BUG+B Air 35-37 


157 "Enterobacter agglomerans" bgp 6 (Pectobacterium) GN-ENT O- Y BUG+B Air 35-37 

158 "Enterobacter agglomerans" bgp 7 (Pantoea) GN-ENT O- Y BUG+B Air 35-37 

159 Enterobacter amnigenus GN-ENT O- Y BUG+B Air 35-37 

160 Enterobacter asburiae GN-ENT O- Y BUG+B Air 35-37 

161 Enterobacter cancerogenus GN-ENT O- Y BUG+B Air 35-37 

162 Enterobacter cloacae GN-ENT O- Y BUG+B Air 35-37 

163 Enterobacter gergoviae GN-ENT O- Y BUG+B Air 35-37 

164 Enterobacter hormaechei GN-ENT O- Y BUG+B Air 35-37 

165 Enterobacter intermedius GN-ENT O- Y BUG+B Air 35-37 

166 Enterobacter nimipressuralis GN-ENT O- Y BUG+B Air 35-37 

167 Enterobacter sakazakii GN-ENT O- Y BUG+B Air 35-37 

168 Erwinia amylovora GN-ENT O- Y BUG+B† Air 30 

169 Escherichia blattae GN-ENT O- Y BUG+B Air 35-37 

170 Escherichia coli GN-ENT O- Y BUG+B Air 35-37 

171 Escherichia coli (USP5-7085) GN-ENT O- Y BUG+B Air 35-37 

172 Escherichia coli inactive GN-ENT O- Y BUG+B Air 35-37 

173 Escherichia coli O157:H7 GN-ENT O- Y BUG+B Air 35-37 

174 Escherichia fergusonii GN-ENT O- Y BUG+B Air 35-37 

175 Escherichia hermannii GN-ENT O- Y BUG+B Air 35-37 

176 Escherichia vulneris GN-ENT O- Y BUG+B Air 35-37 

177 Ewingella americana GN-ENT O- Y BUG+B Air 35-37 

178 Flavimonas oryzihabitans GN-NENT O- N BUG+B Air 30 

179 Flavobacterium ferrugineum GN-NENT O+ N BUG+B Air 30 

180 Flavobacterium flevense GN-NENT O+ N BUG+B Air 30 

181 Flavobacterium hydatis GN-NENT O+ N BUG+B Air 30 

182 Flavobacterium johnsoniae GN-NENT O+ N BUG+B Air 30 

183 Flavobacterium mizutaii GN-NENT O+ N BUG+B Air 30 

184 Flavobacterium mizutaii-like (CDC group II-I) GN-NENT O+ N BUG+B Air 30 

185 Flavobacterium resinovorum GN-NENT O+ N BUG+B Air 30 

186 "Flavobacterium tirrenicum" (Chryseobacterium) GN-NENT O+ N BUG+B Air 30 

187 Francisella philomiragia GN-FAS O-/+ N CHOC 6.5%CO2 35-37 

188 Francisella tularensis GN-FAS O- N CHOC** 6.5%CO2 35-37 

189 Gilardi unnamed rod group 1 GN-NENT O+ N BUG+B Air 30 

190 Haemophilus actinomycetemcomitans GN-FAS O+ Y CHOC 6.5%CO2 35-37 

191 Haemophilus aegyptius GN-FAS O+ Y CHOC 6.5%CO2 35-37 

192 Haemophilus aphrophilus GN-FAS O- Y CHOC 6.5%CO2 35-37 

193 Haemophilus avium (Pasteurella) GN-FAS O+ Y CHOC 6.5%CO2 35-37 




194 Haemophilus ducreyi GN-FAS O+ Y CHOC 6.5%CO2 35-37 

195 Haemophilus haemoglobinophilus GN-FAS O+ Y CHOC 6.5%CO2 35-37 

196 Haemophilus haemolyticus GN-FAS O+ Y CHOC 6.5%CO2 35-37 

197 Haemophilus influenzae GN-FAS O+ Y CHOC 6.5%CO2 35-37 

198 Haemophilus paracuniculus GN-FAS O+ Y CHOC 6.5%CO2 35-37 

199 Haemophilus paragallinarum GN-FAS O- Y CHOC 6.5%CO2 35-37 

200 Haemophilus parahaemolyticus GN-FAS O+ Y CHOC 6.5%CO2 35-37 

201 Haemophilus parainfluenzae GN-FAS O+ Y CHOC 6.5%CO2 35-37 

202 Haemophilus paraphrohaemolyticus GN-FAS O+/- Y CHOC 6.5%CO2 35-37 

203 Haemophilus paraphrophilus GN-FAS O+/- Y CHOC 6.5%CO2 35-37 

204 Haemophilus parasuis GN-FAS O- Y CHOC 6.5%CO2 35-37 

205 Haemophilus segnis GN-FAS O- Y CHOC 6.5%CO2 35-37 

206 Haemophilus somnus GN-FAS O+ Y CHOC 6.5%CO2 35-37 

207 Hafnia alvei GN-ENT O- Y BUG+B Air 35-37 

208 Herbaspirillum rubrisubalbicans GN-NENT O+ N BUG+B Air 30 

209 Herbaspirillum seropedicae GN-NENT O+ N BUG+B Air 30 

210 Hydrogenophaga flava GN-NENT O+ N BUG+B Air 30 

211 Hydrogenophaga palleronii GN-NENT O+ N BUG+B Air 30 

212 Hydrogenophaga pseudoflava GN-NENT O+ N BUG+B Air 30 

213 Hydrogenophaga taeniospiralis GN-NENT O+ N BUG+B Air 30 

214 Iodobacter fluviatilis GN-NENT O+ N BUG+B Air 30 

215 Janthinobacterium lividum GN-NENT O+/- N BUG+B Air 26 

216 Kingella denitirificans GN-FAS O+ Y CHOC 6.5%CO2 35-37 

217 Kingella kingae GN-FAS O+ Y CHOC 6.5%CO2 35-37 

218 Kingella oralis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

219 Klebsiella oxytoca GN-ENT O- Y BUG+B Air 35-37 

220 Klebsiella pneumoniae ss ozaenae GN-ENT O- Y BUG+B Air 35-37 

221 Klebsiella pneumoniae ss pneumoniae GN-ENT O- Y BUG+B Air 35-37 

222 Klebsiella pneumoniae ss rhinoscleromatis GN-ENT O- Y BUG+B Air 35-37 

223 Kluyvera ascorbata GN-ENT O- Y BUG+B Air 35-37 

224 Kluyvera cochleae GN-ENT O- Y BUG+B Air 35-37 

225 Kluyvera cryocrescens GN-ENT O- Y BUG+B Air 35-37 

226 Kluyvera georgiana GN-ENT O- Y BUG+B Air 35-37 

227 Lampropedia hyalina GN-NENT O+ N BUG+B Air 30 

228 Leclercia adecarboxylata GN-ENT O- Y BUG+B Air 35-37 

229 Leminorella grimontii GN-ENT O- Y BUG+B Air 35-37 

230 Leminorella richardii GN-ENT O- Y BUG+B Air 35-37 

231 Listonella anguillarum GN-NENT O+ N BUG+B Air 30 

232 Listonella pelagia GN-NENT O+ N BUG+B Air 30 

233 Mannheimia granulomatis GN-NENT O+ N BUG+B Air 35-37 

234 Mannheimia haemolytica GN-NENT O+ N BUG+B Air 35-37 

235 Moellerella wisconsensis GN-ENT O- Y BUG+B Air 35-37 

236 Moraxella bovis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

237 Moraxella canis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

238 Moraxella caprae GN-FAS O+ Y CHOC 6.5%CO2 35-37 

239 Moraxella catarrhalis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

240 Moraxella caviae GN-FAS O+ Y CHOC 6.5%CO2 35-37 

241 Moraxella cuniculi GN-FAS O+ Y CHOC 6.5%CO2 35-37 

242 Moraxella equi GN-FAS O+ Y CHOC 6.5%CO2 35-37 

243 Moraxella lacunata GN-FAS O+ Y CHOC 6.5%CO2 35-37 

244 Moraxella nonliquefaciens GN-FAS O+ Y CHOC 6.5%CO2 35-37 

245 Moraxella osloensis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

246 Moraxella ovis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

247 Morganella morganii ss morganii GN-ENT O- Y BUG+B Air 35-37 

248 Myroides odoratimimus GN-NENT O+ N BUG+B Air 30 

249 Myroides odoratus GN-NENT O+ N BUG+B Air 30 

250 Neisseria animalis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

251 Neisseria canis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

252 Neisseria cinerea GN-FAS O+ Y CHOC 6.5%CO2 35-37 

253 Neisseria denitrificans GN-FAS O+ Y CHOC 6.5%CO2 35-37 

254 Neisseria elongata ss elongata GN-FAS O+ Y CHOC 6.5%CO2 35-37 

255 Neisseria flava GN-FAS O+ Y CHOC 6.5%CO2 35-37 

256 Neisseria flavescens GN-FAS O+ Y CHOC 6.5%CO2 35-37 

257 Neisseria gonorrhoeae GN-FAS O+ Y CHOC 6.5%CO2 35-37 

258 Neisseria lactamica GN-FAS O+ Y CHOC 6.5%CO2 35-37 

259 Neisseria meningitidis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

260 Neisseria mucosa GN-FAS O+ Y CHOC 6.5%CO2 35-37 

261 Neisseria perflava GN-FAS O+ Y CHOC 6.5%CO2 35-37 








262 Neisseria sicca GN-FAS O+ Y CHOC 6.5%CO2 35-37 

263 Neisseria subflava GN-FAS O+ Y CHOC 6.5%CO2 35-37 

264 Neisseria weaveri GN-FAS O+ Y CHOC 6.5%CO2 35-37 

265 Obesumbacterium proteus GN-ENT O- Y BUG+B Air 35-37 

266 Obesumbacterium proteus biogroup 2 GN-ENT O- Y BUG+B Air 35-37 

267 Oceanomonas doudoroffii GN-NENT O+ N BUG+B† Air 30 

268 Ochrobactrum anthropi GN-NENT O+ N BUG+B Air 30 

269 Oligella ureolytica GN-NENT O+ N BUG+B Air 35-37 

270 Oligella urethralis GN-NENT O+ N BUG+B Air 35-37 

271 Ornithobacterium rhinotracheale GN-NENT O+ N BUG+B Air 30 

272 Pandoraea norimbergensis GN-NENT O+ N BUG+B† Air 30 

273 Pantoea agglomerans GN-ENT O- Y BUG+B Air 35-37 

274 Pantoea citrea GN-ENT O- Y BUG+B Air 35-37 

275 Pantoea dispersa GN-ENT O- Y BUG+B Air 35-37 

276 Pantoea punctata GN-ENT O- Y BUG+B Air 35-37 

277 Pantoea stewartii ss stewartii GN-ENT O- Y BUG+B Air 35-37 

278 Pantoea terrea GN-ENT O- Y BUG+B Air 35-37 

279 "Pasteurella" aerogenes GN-NENT O+/- N BUG+B Air 35-37 

280 Pasteurella anatis GN-NENT O+/- N BUG+B Air 35-37 

281 Pasteurella bettyae GN-NENT O- N BUG+B Air 35-37 

282 Pasteurella caballi GN-NENT O+ N BUG+B Air 35-37 

283 Pasteurella canis/stomatis GN-NENT O+ N BUG+B Air 35-37 

284 Pasteurella dagmatis GN-NENT O+ N BUG+B Air 35-37 

285 Pasteurella gallinarum GN-NENT O+ N BUG+B Air 35-37 

286 Pasteurella langaaensis GN-NENT O+/- N BUG+B Air 35-37 

287 Pasteurella lymphangitidis GN-NENT O- N BUG+B Air 30 

288 Pasteurella mairii GN-NENT O+ N BUG+B Air 30 

289 Pasteurella multocida ss multocida GN-NENT O+ N BUG+B Air 35-37 

290 Pasteurella pneumotropica GN-NENT O+ N BUG+B Air 35-37 

291 "Pasteurella" testudinis GN-NENT O+ N BUG+B Air 30 

292 Pasteurella trehalosi GN-NENT O+ N BUG+B Air 30 

293 Pasteurella volantium GN-NENT O+ N BUG+B Air 35-37 

294 Paucimonas lemoignei GN-NENT O+ N BUG+B† Air 30 

295 Pectobacterium carotovorum ss atrosepticum GN-ENT O- Y BUG+B† Air 35-37 

296 Pectobacterium carotovorum ss betavasculorum GN-ENT O- Y BUG+B† Air 35-37 

297 Pectobacterium carotovorum ss carotovorum GN-ENT O- Y BUG+B† Air 35-37 

298 Pectobacterium chrysanthemi GN-ENT O- Y BUG+B† Air 35-37 

299 Pectobacterium cypripedii GN-ENT O- Y BUG+B† Air 35-37 

300 Pedobacter heparinus GN-NENT O+ N BUG+B Air 30 

301 Photobacterium angustum GN-NENT O+ N BUG+B Air 30 

302 Photobacterium damsela ss damselae GN-NENT O+ N BUG+B Air 30 

303 Photobacterium fischeri GN-NENT O+ N BUG+B Air 30 

304 Photobacterium leiognathi GN-NENT O+ N BUG+B Air 30 

305 Photorhabdus luminescens ss luminescens GN-ENT O- N BUG+B Air 30 

306 Phyllobacterium myrsinacearum GN-NENT O+ N BUG+B Air 30 

307 Phyllobacterium rubiacearum GN-NENT O+ N BUG+B Air 30 

308 Plesiomonas shigelloides GN-NENT O+ N BUG+B Air 30 

309 Pragia fontium GN-ENT O- Y BUG+B Air 35-37 

310 Proteus mirabilis GN-ENT O- Y BUG+B Air 35-37 

311 Proteus myxofaciens GN-ENT O- Y BUG+B Air 35-37 

312 Proteus penneri/vulgaris GN-ENT O- Y BUG+B Air 35-37 

313 Providencia alcalifaciens GN-ENT O- Y BUG+B Air 35-37 

314 Providencia heimbachae GN-ENT O- Y BUG+B Air 35-37 

315 Providencia rettgeri GN-ENT O- Y BUG+B Air 35-37 

316 Providencia rustigianii GN-ENT O- Y BUG+B Air 35-37 

317 Providencia stuartii GN-ENT O- Y BUG+B Air 35-37 

318 Pseudomonas aeruginosa GN-NENT O+ N BUG+B† Air 30 

319 Pseudomonas agarici GN-NENT O+ N BUG+B† Air 30 

320 Pseudomonas alcaligenes GN-NENT O+/- N BUG+B† Air 30 

321 Pseudomonas asplenii GN-NENT O+ N BUG+B† Air 30 

322 Pseudomonas aurantiaca GN-NENT O+ N BUG+B† Air 30 

323 "Pseudomonas bathycetes" GN-NENT O+ N BUG+B† Air 30 

324 "Pseudomonas" boreopolis (Stenotrophomonas) GN-NENT O+ N BUG+B† Air 30 

325 Pseudomonas caricapapayae (syringae) GN-NENT O- N BUG+B† Air 30 

326 Pseudomonas chlororaphis GN-NENT O+ N BUG+B† Air 30 

327 Pseudomonas cichorii (syringae) GN-NENT O- N BUG+B† Air 30 

328 "Pseudomonas" cissicola (Xanthomonas-like) GN-NENT O- N BUG+B† Air 30 

329 Pseudomonas citronellolis GN-NENT O+ N BUG+B† Air 30 




330 Pseudomonas corrugata GN-NENT O+ N BUG+B† Air 30 

331 "Pseudomonas floridana" (Burkholderia-like) GN-NENT O+ N BUG+B† Air 30 

332 Pseudomonas fluorescens GN-NENT O+/- N BUG+B† Air 30 

333 Pseudomonas fluorescens biotype A GN-NENT O+/- N BUG+B† Air 30 

334 Pseudomonas fluorescens biotype C GN-NENT O+/- N BUG+B† Air 30 

335 Pseudomonas fluorescens biotype F GN-NENT O+/- N BUG+B† Air 30 

336 Pseudomonas fluorescens biotype G GN-NENT O+/- N BUG+B† Air 30 

337 Pseudomonas fragi GN-NENT O+ N BUG+B† Air 30 

338 Pseudomonas fulva GN-NENT O+ N BUG+B† Air 30 

339 Pseudomonas fuscovaginae GN-NENT O+ N BUG+B† Air 30 

340 "Pseudomonas group 2" (Burkholderia-like) GN-NENT O+ N BUG+B† Air 30 

341 "Pseudomonas" huttiensis (Burkholderia-like) GN-NENT O+ N BUG+B† Air 30 

342 Pseudomonas lundensis GN-NENT O+ N BUG+B† Air 30 

343 Pseudomonas "maculicola" GN-NENT O+ N BUG+B† Air 30 

344 Pseudomonas marginalis GN-NENT O+ N BUG+B† Air 30 

345 Pseudomonas mendocina GN-NENT O+ N BUG+B† Air 30 

346 "Pseudomonas mephitica" GN-NENT O+ N BUG+B† Air 30 

347 Pseudomonas mucidolens GN-NENT O+ N BUG+B† Air 30 

348 Pseudomonas nitroreducens/azelaica GN-NENT O+ N BUG+B† Air 30 

349 Pseudomonas oleovorans GN-NENT O+ N BUG+B† Air 30 

350 Pseudomonas pertucinogena GN-NENT O+ N BUG+B† Air 30 

351 Pseudomonas pseudoalcaligenes ss pseudoalcaligenes GN-NENT O+ N BUG+B† Air 30 

352 Pseudomonas putida GN-NENT O+ N BUG+B† Air 30 

353 Pseudomonas putida biotype A GN-NENT O+ N BUG+B† Air 30 

354 Pseudomonas putida biotype B GN-NENT O+ N BUG+B† Air 30 

355 Pseudomonas resinovorans GN-NENT O+ N BUG+B† Air 30 

356 Pseudomonas savastanoi pv fraxini GN-NENT O- N BUG+B† Air 30 

357 Pseudomonas savastanoi pv glycinea GN-NENT O- N BUG+B† Air 30 

358 Pseudomonas savastanoi pv nerii GN-NENT O- N BUG+B† Air 30 

359 Pseudomonas spinosa (Burkholderia) GN-NENT O+ N BUG+B† Air 30 

360 Pseudomonas straminea GN-NENT O+ N BUG+B† Air 30 

361 Pseudomonas stutzeri GN-NENT O+ N BUG+B† Air 30 

362 Pseudomonas synxantha GN-NENT O+ N BUG+B† Air 30 

363 Pseudomonas syringae pv aceris GN-NENT O- N BUG+B† Air 30 

364 Pseudomonas syringae pv antirrhini GN-NENT O- N BUG+B† Air 30 

365 Pseudomonas syringae pv apii GN-NENT O- N BUG+B† Air 30 

366 Pseudomonas syringae pv aptata GN-NENT O- N BUG+B† Air 30 

367 Pseudomonas syringae pv atrofaciens GN-NENT O- N BUG+B† Air 30 

368 Pseudomonas syringae pv coronafaciens GN-NENT O- N BUG+B† Air 30 

369 Pseudomonas syringae pv cunninghamiae GN-NENT O- N BUG+B† Air 30 

370 Pseudomonas syringae pv delphinii GN-NENT O- N BUG+B† Air 30 

371 Pseudomonas syringae pv eriobotryae GN-NENT O- N BUG+B† Air 30 

372 Pseudomonas syringae pv glycinea GN-NENT O- N BUG+B† Air 30 

373 Pseudomonas syringae pv helianthi GN-NENT O- N BUG+B† Air 30 

374 Pseudomonas syringae pv lachrymans GN-NENT O- N BUG+B† Air 30 

375 Pseudomonas syringae pv mori GN-NENT O- N BUG+B† Air 30 

376 Pseudomonas syringae pv myricae GN-NENT O- N BUG+B† Air 30 

377 Pseudomonas syringae pv oryzae GN-NENT O- N BUG+B† Air 30 

378 Pseudomonas syringae pv papulans GN-NENT O- N BUG+B† Air 30 

379 Pseudomonas syringae pv persicae GN-NENT O- N BUG+B† Air 30 

380 Pseudomonas syringae pv phaseolicola GN-NENT O- N BUG+B† Air 30 

381 Pseudomonas syringae pv pisi GN-NENT O- N BUG+B† Air 30 

382 Pseudomonas syringae pv porri GN-NENT O- N BUG+B† Air 30 

383 Pseudomonas syringae pv primulae GN-NENT O- N BUG+B† Air 30 

384 Pseudomonas syringae pv sesami GN-NENT O- N BUG+B† Air 30 

385 Pseudomonas syringae pv syringae GN-NENT O- N BUG+B† Air 30 

386 Pseudomonas syringae pv tabaci A GN-NENT O- N BUG+B† Air 30 

387 Pseudomonas syringae pv tabaci B GN-NENT O- N BUG+B† Air 30 

388 Pseudomonas syringae pv tagetis GN-NENT O- N BUG+B† Air 30 

389 Pseudomonas syringae pv tomato GN-NENT O- N BUG+B† Air 30 

390 Pseudomonas syringae pv zinaniae GN-NENT O- N BUG+B† Air 30 

391 Pseudomonas taetrolens GN-NENT O+ N BUG+B† Air 30 

392 Pseudomonas tolaasii GN-NENT O+ N BUG+B† Air 30 

393 Pseudomonas viridiflava (syringae) GN-NENT O- N BUG+B† Air 30 

394 Pseudomonas viridilivida GN-NENT O- N BUG+B† Air 30 

395 Psychrobacter immobilis GN-NENT O+ N BUG+B Air 30 

396 Psychrobacter phenylpyruvicus GN-NENT O+ Y BUG+B Air 35-37 

397 Rahnella aquatilis GN-ENT O- Y BUG+B Air 35-37 








398 Ralstonia eutropha GN-NENT O+ N BUG+B Air 30 

399 Ralstonia paucula GN-NENT O+ N BUG+B Air 30 

400 Ralstonia pickettii GN-NENT O+ N BUG+B Air 30 

401 Ralstonia solanacearum GN-NENT O+ N BUG+B Air 30 

402 Raoultella planticola GN-ENT O- Y BUG+B Air 35-37 

403 Raoultella planticola/ornithinolytica GN-ENT O- Y BUG+B Air 35-37 

404 Raoultella terrigena GN-ENT O- Y BUG+B Air 35-37 

405 Rhizobium like-Cystic Fibrosis GN-NENT O+ N BUG+B† Air 35-37 

406 Rhizobium radiobacter GN-NENT O+/- N BUG+B† Air 30 

407 Rhizobium rhizogenes GN-NENT O+/- N BUG+B† Air 30 

408 Rhizobium vitis GN-NENT O+/- N BUG+B† Air 30 

409 Riemerella anatipestifer GN-NENT O+ N BUG+B Air 35-37 

410 Roseomonas cervicalis GN-NENT O+ N BUG+B Air 30 

411 Roseomonas fauriae GN-NENT O+ N BUG+B Air 30 

412 Roseomonas genomospecies 4 GN-NENT O+ N BUG+B Air 30 



415 Roseomonas gilardii GN-NENT O+ N BUG+B Air 30 

416 Salmonella gp 1 (choleraesuis) GN-ENT O- Y BUG+B Air 35-37 

417 Salmonella gp 1 (choleraesuis) st choleraesuis GN-ENT O- Y BUG+B Air 35-37 

418 Salmonella gp 1 (choleraesuis) st gallinarum GN-ENT O- Y BUG+B Air 35-37 

419 Salmonella gp 1 (choleraesuis) st paratyphi A GN-ENT O- Y BUG+B Air 35-37 

420 Salmonella gp 1 (choleraesuis) st pullorum GN-ENT O- Y BUG+B Air 35-37 

421 Salmonella gp 1 (choleraesuis) st typhi GN-ENT O- Y BUG+B Air 35-37 

422 Salmonella gp 1 (choleraesuis) st typhimurium GN-ENT O- Y BUG+B Air 35-37 

423 Salmonella gp 3a (arizonae) GN-ENT O- Y BUG+B Air 35-37 

424 Salmonella gp 3b (diarizonae) GN-ENT O- Y BUG+B Air 35-37 

425 Salmonella gp 4 (houtenae) GN-ENT O- Y BUG+B Air 35-37 

426 Salmonella gp 5 (bongori) GN-ENT O- Y BUG+B Air 35-37 

427 Salmonella gp 6 (indica) GN-ENT O- Y BUG+B Air 35-37 

428 Serpens flexibilis GN-NENT O+ N BUG+B Air 30 

429 Serratia entomophila GN-ENT O- Y BUG+B Air 35-37 

430 Serratia ficaria GN-ENT O-/v Y BUG+B Air 35-37 

431 Serratia fonticola GN-ENT O- Y BUG+B Air 35-37 

432 Serratia liquefaciens/grimesii GN-ENT O- Y BUG+B Air 35-37 

433 Serratia marcescens GN-ENT O- Y BUG+B Air 35-37 

434 Serratia odorifera GN-ENT O- Y BUG+B Air 35-37 

435 Serratia plymuthica GN-ENT O- Y BUG+B Air 35-37 

436 Serratia proteamaculans ss proteamaculans GN-ENT O- Y BUG+B Air 35-37 

437 Serratia rubidaea GN-ENT O- Y BUG+B Air 35-37 

438 Shewanella algae GN-NENT O+ N BUG+B Air 30 

439 Shewanella putrefaciens A GN-NENT O+ N BUG+B Air 30 

440 Shewanella putrefaciens B GN-NENT O+ N BUG+B Air 30 

441 Shigella boydii GN-ENT O- Y BUG+B Air 35-37 

442 Shigella dysenteriae GN-ENT O- Y BUG+B Air 35-37 

443 Shigella flexneri GN-ENT O- Y BUG+B Air 35-37 

444 Shigella sonnei GN-ENT O- Y BUG+B Air 35-37 

445 Simonsiella crassa GN-FAS O+ Y CHOC 6.5%CO2 35-37 

446 Simonsiella muelleri GN-FAS O+ Y CHOC 6.5%CO2 35-37 

447 Simonsiella steedae GN-FAS O+ Y CHOC 6.5%CO2 35-37 

448 Sinorhizobium meliloti GN-NENT O+ N BUG+B Air 30 

449 Sphingobacterium multivorum GN-NENT O+ N BUG+B Air 30 

450 Sphingobacterium multivorum-like GN-NENT O+ N BUG+B Air 30 

451 Sphingobacterium spiritivorum GN-NENT O+ N BUG+B Air 30 

452 Sphingobacterium thalpophilum GN-NENT O+ N BUG+B Air 30 

453 Sphingomonas adhaesiva GN-NENT O+/- N BUG+B Air 30 

454 Sphingomonas capsulata GN-NENT O+/- N BUG+B Air 30 

455 Sphingomonas echinoides GN-NENT O+ N BUG+B† Air 30 

456 Sphingomonas macrogoltabidus GN-NENT O+/- N BUG+B Air 30 

457 Sphingomonas parapaucimobilis GN-NENT O+/- N BUG+B Air 30 

458 Sphingomonas paucimobilis A GN-NENT O+/- N BUG+B Air 30 

459 Sphingomonas paucimobilis B GN-NENT O+/- N BUG+B Air 30 

460 Sphingomonas sanguinis GN-NENT O+/- N BUG+B Air 30 

461 Sphingomonas terrae GN-NENT O+/- N BUG+B Air 30 

462 Sphingomonas yanoikuyae GN-NENT O+/- N BUG+B Air 30 

463 Stenotrophomonas maltophilia GN-NENT O- N BUG+B† Air 30 

464 Suttonella indologenes GN-FAS O+ Y CHOC 6.5%CO2 35-37 

465 Tatumella ptyseos GN-ENT O- Y BUG+B Air 35-37 




466 Taylorella equigenitalis GN-FAS O+ Y CHOC 6.5%CO2 35-37 

467 Trabulsiella guamensis GN-ENT O- Y BUG+B Air 35-37 

468 Variovorax paradoxus GN-NENT O+ N BUG+B Air 30 

469 Vibrio aestuarianus GN-NENT O+ N BUG+B Air 30 

470 Vibrio alginolyticus GN-NENT O+ N BUG+B† Air 30 

471 Vibrio campbelli GN-NENT O+ N BUG+B Air 30 

472 Vibrio carchariae GN-NENT O+ N BUG+B Air 30 

473 Vibrio cholerae O1 (ATCC 25870) GN-NENT O+ N BUG+B Air 30 

474 Vibrio cholerae O1/0139 GN-NENT O+ N BUG+B Air 30 

475 Vibrio cholerae non O1 GN-NENT O+ N BUG+B Air 30 

476 Vibrio cincinnatiensis GN-NENT O+ N BUG+B Air 30 

477 Vibrio diazotrophicus GN-NENT O+ N BUG+B Air 30 

478 Vibrio fluvialis GN-NENT O+ N BUG+B Air 30 

479 Vibrio furnissii GN-NENT O+ N BUG+B Air 30 

480 Vibrio harveyi GN-NENT O+ N BUG+B Air 30 

481 Vibrio mediterranei GN-NENT O+ N BUG+B Air 30 

482 Vibrio metschnikovii GN-NENT O- N BUG+B Air 30 

483 Vibrio mimicus GN-NENT O+ N BUG+B Air 30 

484 Vibrio natriegens GN-NENT O+ N BUG+B Air 30 

485 Vibrio ordalii GN-NENT O+ N BUG+B Air 30 

486 Vibrio parahaemolyticus GN-NENT O+ N BUG+B Air 30 

487 Vibrio proteolyticus GN-NENT O+ N BUG+B Air 30 

488 Vibrio splendidus GN-NENT O+ N BUG+B Air 26 

489 Vibrio tubiashii GN-NENT O+ N BUG+B Air 30 

490 Vibrio vulnificus GN-NENT O+ N BUG+B Air 30 

491 Vogesella indigofera GN-NENT O+ N BUG+B Air 30 

492 Weeksella virosa GN-NENT O+ N BUG+B Air 30 

493 Xanthomonas albilineans GN-NENT O- N BUG Air 30 

494 Xanthomonas campestris pv begoniae A GN-NENT O- N BUG Air 30 

495 Xanthomonas campestris pv begoniae B GN-NENT O- N BUG Air 30 

496 Xanthomonas campestris pv campestris GN-NENT O- N BUG Air 30 

497 Xanthomonas campestris pv carotae GN-NENT O- N BUG Air 30 

498 Xanthomonas campestris pv dieffenbachiae GN-NENT O- N BUG Air 30 

499 Xanthomonas campestris pv hyacinthi GN-NENT O- N BUG Air 30 

500 Xanthomonas campestris pv juglandis GN-NENT O- N BUG Air 30 

501 Xanthomonas campestris pv malvacearum GN-NENT O- N BUG Air 30 

502 Xanthomonas campestris pv nigromaculans GN-NENT O- N BUG Air 30 

503 Xanthomonas campestris pv pelargonii GN-NENT O- N BUG Air 30 

504 Xanthomonas campestris pv phaseoli GN-NENT O- N BUG Air 30 

505 Xanthomonas campestris pv poinsettiicola GN-NENT O- N BUG Air 30 

506 Xanthomonas campestris pv raphani GN-NENT O- N BUG Air 30 

507 Xanthomonas campestris pv tardicrescens GN-NENT O- N BUG Air 30 

508 Xanthomonas campestris pv translucens GN-NENT O- N BUG Air 30 

509 Xanthomonas campestris pv vesicatoria GN-NENT O- N BUG Air 30 

510 Xanthomonas oryzae pv oryzicola GN-NENT O- N BUG Air 30 

511 Xenorhabdus bovienii GN-ENT O- N BUG+B Air 30 

512 Xenorhabdus nematophila GN-ENT O- N BUG+B Air 30 

513 Yersinia aldovae GN-ENT O- Y BUG+B Air 35-37 

514 Yersinia bercovieri GN-ENT O- Y BUG+B Air 35-37 

515 Yersinia enterocolitica ss enterocolitica GN-ENT O- Y BUG+B Air 35-37 

516 Yersinia fredericksenii GN-ENT O- Y BUG+B Air 35-37 

517 Yersinia intermedia GN-ENT O- Y BUG+B Air 35-37 

518 Yersinia kristensenii GN-ENT O- Y BUG+B Air 35-37 

519 Yersinia mollaretii GN-ENT O- Y BUG+B Air 35-37 

520 Yersinia pestis GN-ENT O- N BUG+B** Air 35-37 

521 Yersinia pseudotuberculosis GN-ENT O- Y BUG+B Air 35-37 

522 Yersinia rohdei GN-ENT O- Y BUG+B Air 35-37 

523 Yersinia ruckeri GN-ENT O- Y BUG+B Air 35-37 

524 Yokenella regensburgei GN-ENT O- Y BUG+B Air 35-37 




Gram-Positive Aerobic Bacteria 





1 Actinomyces bovis GP-ROD C- Y BUG+B 6.5%CO2 35-37 

2 Actinomyces canis GP-ROD C+/- Y BUG+B 6.5%CO2 35-37 

3 Actinomyces hordeovulneris GP-ROD C- Y BUG+B 6.5%CO2 35-37 

4 Actinomyces hyovaginalis GP-ROD C-/W+ Y BUG+B 6.5%CO2 35-37 

5 Actinomyces naeslundii GP-ROD C-/W+ Y BUG+B 6.5%CO2 35-37 

6 Actinomyces neuii ss anitratus GP-ROD C+/- Y BUG+B 6.5%CO2 35-37 

7 Actinomyces neuii ss neuii GP-ROD C+/- Y BUG+B 6.5%CO2 35-37 

8 Actinomyces odontolyticus GP-ROD C- Y BUG+B 6.5%CO2 35-37 

9 Actinomyces radingae/turicensis (CDC.E) GP-ROD C- Y BUG+B 6.5%CO2 35-37 

10 Actinomyces viscosus GP-ROD C+ Y BUG+B 6.5%CO2 35-37 

11 Aerococcus christensenii GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

12 Aerococcus urinae GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

13 Aerococcus viridans GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

14 Alloiococcus otitis GP-COCCUS C-/W+ Y BUG+B 6.5%CO2 35-37 

15 Arcanobacterium bernardiae (CDC.2) GP-ROD C- Y BUG+B 6.5%CO2 35-37 

16 Arcanobacterium haemolyticum GP-ROD C- Y BUG+B 6.5%CO2 35-37 

17 Arcanobacterium pyogenes GP-ROD C- Y BUG+B 6.5%CO2 35-37 

18 Arthrobacter cumminsii GP-ROD C+ Y BUG+B Air 30 

19 Arthrobacter histidinolovorans GP-ROD C+ Y BUG+B Air 30 

20 Arthrobacter ilicis GP-ROD C+ Y BUG+B Air 30 

21 Arthrobacter woluwensis GP-ROD C+ Y BUG+B Air 30 

22 Aureobacterium resistens GP-ROD C+ Y BUG+B Air 35-37 

23 Bacillus alcalophilus GP-ROD SB C-/W+ N BUG+M Air 30 

24 Bacillus amyloliquefaciens A GP-ROD SB C-/W+ N BUG+M Air 30 

25 Bacillus amyloliquefaciens B GP-ROD SB C-/W+ N BUG+M Air 30 

26 Bacillus anthracis subgroup A GP-ROD SB C-/W+ N BUG+M** Air 30 

27 Bacillus anthracis subgroup B GP-ROD SB C-/W+ N BUG+M** Air 30 

28 Bacillus anthracis subgroup C GP-ROD SB C-/W+ N BUG+M** Air 30 

29 Bacillus anthracis subgroup D GP-ROD SB C-/W+ N BUG+M** Air 30 

30 Bacillus badius GP-ROD SB C-/W+ N BUG+M Air 30 

31 Bacillus cereus/thuringiensis A GP-ROD SB C-/W+ N BUG+M Air 30 

32 Bacillus cereus/thuringiensis B GP-ROD SB C-/W+ N BUG+M Air 30 

33 Bacillus cereus/thuringiensis C GP-ROD SB C-/W+ N BUG+M Air 30 

34 Bacillus circulans GP-ROD SB C-/W+ N BUG+M Air 30 

35 Bacillus coagulans GP-ROD SB C-/W+ N BUG+M Air 30 

36 Bacillus fastidiosus GP-ROD SB C-/W+ N BUG+M Air 30 

37 Bacillus firmus GP-ROD SB C-/W+ N BUG+M Air 30 

38 Bacillus halodurans GP-ROD SB C-/W+ N BUG+M Air 30 

39 Bacillus laevolacticus GP-ROD SB C-/W+ N BUG+M Air 30 

40 Bacillus licheniformis GP-ROD SB C-/W+ N BUG+M Air 30 

41 Bacillus maroccanus GP-ROD SB C-/W+ N BUG+M Air 30 

42 Bacillus megaterium A GP-ROD SB C-/W+ N BUG+M Air 30 

43 Bacillus megaterium B GP-ROD SB C-/W+ N BUG+M Air 30 

44 Bacillus mycoides GP-ROD SB C-/W+ N BUG+M Air 30 

45 Bacillus psychrosaccharolyticus GP-ROD SB C-/W+ N BUG+M Air 30 

46 Bacillus pumilus A GP-ROD SB C-/W+ N BUG+M Air 30 

47 Bacillus pumilus B GP-ROD SB C-/W+ N BUG+M Air 30 

48 Bacillus pumilus C GP-ROD SB C-/W+ N BUG+M Air 30 

49 Bacillus racemilacticus GP-ROD SB C-/W+ N BUG+M Air 30 

50 Bacillus sphaericus GP-ROD SB C-/W+ N BUG+M Air 30 

51 Bacillus subtilis A GP-ROD SB C-/W+ N BUG+M Air 30 

52 Bacillus subtilis B GP-ROD SB C-/W+ N BUG+M Air 30 

53 Bacillus subtilis C GP-ROD SB C-/W+ N BUG+M Air 30 

54 Brevibacillus brevis GP-ROD SB C-/W+ N BUG+M Air 30 

55 Brevibacterium casei GP-ROD C+ Y BUG+B Air 35-37 

56 Brevibacterium epidermidis GP-ROD C+ Y BUG+B Air 35-37 

57 Brevibacterium linens GP-ROD C+ Y BUG+B Air 35-37 

58 Brevibacterium liquifaciens GP-ROD C+ Y BUG+B Air 35-37 

59 Brevibacterium mcbrellneri GP-ROD C+ Y BUG+B Air 35-37 

60 Brevibacterium otitidis GP-ROD C+ Y BUG+B Air 35-37 

61 Brochothrix campestris GP-ROD C+ Y BUG+B 6.5%CO2 35-37 

62 Brochothrix thermosphacta GP-ROD C+ Y BUG+B Air 30 

63 Carnobacterium alterfunditum GP-ROD C- Y BUG+B Air 26 

64 Carnobacterium divergens GP-ROD C- Y BUG+B Air 26 




65 Carnobacterium gallinarum GP-ROD C- Y BUG+B Air 26 

66 Carnobacterium mobile GP-ROD C- Y BUG+B Air 26 

67 Carnobacterium piscicola GP-ROD C- Y BUG+B Air 26 

68 Cellulomonas biazotea GP-ROD C+ Y BUG+B Air 35-37 

69 Cellulomonas cellasea GP-ROD C+ Y BUG+B Air 30 

70 Cellulomonas fimi GP-ROD C+ Y BUG+B Air 35-37 

71 Cellulomonas flavigena GP-ROD C+ Y BUG+B Air 35-37 

72 Cellulomonas gelida GP-ROD C+ Y BUG+B Air 35-37 

73 Cellulomonas hominis (CDC.A-3) GP-ROD C+ Y BUG+B Air 35-37 

74 Cellulomonas turbata (Oerskovia turbata) GP-ROD C+ Y BUG+B Air 35-37 

75 Cellulomonas uda GP-ROD C+ Y BUG+B Air 35-37 

76 Cellulosimicrobium cellulans GP-ROD C+ Y BUG+B Air 35-37 

77 Clavibacter agropyri (Corynebacterium) GP-ROD C+ N BUG Air 30 

78 Clavibacter michiganensis ss insidiosus GP-ROD C+ N BUG Air 30 

79 Clavibacter michiganensis ss michiganensis GP-ROD C+ N BUG Air 30 

80 Clavibacter michiganensis ss nebraskensis GP-ROD C+ N BUG Air 30 

81 Clavibacter michiganensis ss sepedonicus GP-ROD C+ N BUG Air 30 

82 Clavibacter michiganensis ss tessellarius GP-ROD C+ N BUG Air 30 

83 Corynebacterium accolens GP-ROD C+ Y BUG+B Air 35-37 

84 Corynebacterium afermentans ss afermentans(CDC.ANF-1) GP-ROD C+ Y BUG+B Air 35-37 

85 Corynebacterium afermentans ss lipophilum GP-ROD C+ Y BUG+B Air 35-37 

86 "Corynebacterium" ammoniagenes (Brevibacterium-like) GP-ROD C+ Y BUG+B Air 35-37 

87 Corynebacterium amycolatum (CDC.F-2) GP-ROD C+ Y BUG+B Air 35-37 

88 Corynebacterium argentoratense GP-ROD C+ Y BUG+B Air 35-37 

89 Corynebacterium auris GP-ROD C+ Y BUG+B Air 35-37 

90 Corynebacterium bovis GP-ROD C+ Y BUG+B Air 35-37 

91 Corynebacterium callunae GP-ROD C+ Y BUG+B Air 35-37 

92 Corynebacterium camporrealensis GP-ROD C+ Y BUG+B Air 35-37 

93 Corynebacterium coyleae GP-ROD C+ Y BUG+B Air 35-37 

94 Corynebacterium cystitidis GP-ROD C+ Y BUG+B Air 35-37 

95 Corynebacterium diphtheriae GP-ROD C+ Y BUG+B Air 35-37 

96 Corynebacterium durum GP-ROD C+ Y BUG+B Air 35-37 

97 Corynebacterium falsenii GP-ROD C+ Y BUG+B Air 35-37 

98 Corynebacterium flavescens GP-ROD C+ Y BUG+B Air 35-37 

99 Corynebacterium glucuronolyticum GP-ROD C+ Y BUG+B Air 35-37 

100 Corynebacterium glutamicum GP-ROD C+ Y BUG+B Air 35-37 

101 Corynebacterium imitans GP-ROD C+ Y BUG+B Air 35-37 

102 Corynebacterium jeikeium GP-ROD C+ Y BUG+B Air 35-37 

103 Corynebacterium kutscheri GP-ROD C+ Y BUG+B Air 35-37 

104 Corynebacterium lipophiloflavum GP-ROD C+ Y BUG+B Air 35-37 

105 Corynebacterium macginleyi GP-ROD C+ Y BUG+B Air 35-37 

106 Corynebacterium mastitidis GP-ROD C+ Y BUG+B Air 35-37 

107 Corynebacterium matruchotii GP-ROD C+ Y BUG+B Air 35-37 

108 Corynebacterium minutissimum GP-ROD C+ Y BUG+B Air 35-37 

109 Corynebacterium mucifaciens GP-ROD C+ Y BUG+B Air 35-37 

110 Corynebacterium mycetoides GP-ROD C+ Y BUG+B Air 35-37 

111 "Corynebacterium nitrilophilus" GP-ROD C+ Y BUG+B Air 35-37 

112 Corynebacterium pilosum GP-ROD C+ Y BUG+B Air 35-37 

113 Corynebacterium pseudodiphtheriticum/propinquum(CDC.ANF-3) GP-ROD C+ Y BUG+B Air 35-37 

114 Corynebacterium pseudotuberculosis GP-ROD C+ Y BUG+B Air 35-37 

115 Corynebacterium renale GP-ROD C+ Y BUG+B Air 35-37 

116 Corynebacterium riegelii GP-ROD C+ Y BUG+B Air 35-37 

117 Corynebacterium seminale GP-ROD C+ Y BUG+B Air 35-37 

118 Corynebacterium singulare GP-ROD C+ Y BUG+B Air 35-37 

119 Corynebacterium spp. (CDC.G) GP-ROD C+ Y BUG+B Air 35-37 

120 Corynebacterium striatum (CDC.I-1) GP-ROD C+ Y BUG+B Air 35-37 

121 Corynebacterium thomssenii GP-ROD C+ Y BUG+B Air 35-37 

122 Corynebacterium ulcerans GP-ROD C+ Y BUG+B 6.5%CO2 35-37 

123 Corynebacterium urealyticum GP-ROD C+ Y BUG+B Air 35-37 

124 Corynebacterium variabile (Caseobacter polymorphus) GP-ROD C+ Y BUG+B Air 35-37 

125 Corynebacterium vitaeruminis GP-ROD C+ Y BUG+B Air 35-37 

126 "Corynebacterium" xerosis (GPC) GP-COCCUS C+ Y BUG+B Air 35-37 

127 Curtobacterium albidum GP-ROD C+ N BUG Air 30 

128 Curtobacterium citreum GP-ROD C+ N BUG Air 30 

129 Curtobacterium flaccumfaciens GP-ROD C+ N BUG Air 30 

130 Curtobacterium luteum GP-ROD C+ N BUG Air 30 

131 Curtobacterium pusillum GP-ROD C+ N BUG Air 30 

132 Deinococcus grandis GP-COCCUS C+ Y BUG+B Air 30 








133 Deinococcus proteolyticus GP-COCCUS C+ Y BUG+B Air 30 

134 Deinococcus radiodurans GP-COCCUS C+ Y BUG+B Air 30 

135 Deinococcus radiophilus GP-COCCUS C+ Y BUG+B Air 30 

136 Deinococcus radiopugnans GP-COCCUS C+ Y BUG+B Air 35-37 

137 Dermabacter hominis GP-ROD C+ Y BUG+B Air 35-37 

138 Dermacoccus nishinomiyaensis GP-COCCUS C+ Y BUG+B Air 30 

139 Dolosicoccus paucivorans GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

140 Dolosigranulum pigrum GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

141 Enterococcus avium GP-COCCUS C- Y BUG+B Air 35-37 

142 Enterococcus casseliflavus GP-COCCUS C- Y BUG+B Air 35-37 

143 Enterococcus cecorum GP-COCCUS C- Y BUG+B Air 35-37 

144 Enterococcus columbae GP-COCCUS C- Y BUG+B Air 35-37 

145 Enterococcus dispar GP-COCCUS C- Y BUG+B Air 30 

146 Enterococcus durans GP-COCCUS C- Y BUG+B Air 35-37 

147 Enterococcus faecalis GP-COCCUS C- Y BUG+B Air 35-37 

148 Enterococcus faecium GP-COCCUS C- Y BUG+B Air 35-37 

149 Enterococcus flavescens GP-COCCUS C- Y BUG+B Air 35-37 

150 Enterococcus gallinarum GP-COCCUS C- Y BUG+B Air 35-37 

151 Enterococcus hirae GP-COCCUS C- Y BUG+B Air 35-37 

152 Enterococcus malodoratus GP-COCCUS C- Y BUG+B Air 35-37 

153 Enterococcus mundtii GP-COCCUS C- Y BUG+B Air 35-37 

154 Enterococcus pseudoavium GP-COCCUS C- Y BUG+B Air 35-37 

155 Enterococcus raffinosus GP-COCCUS C- Y BUG+B Air 35-37 

156 Enterococcus saccharolyticus GP-COCCUS C- Y BUG+B Air 35-37 

157 Enterococcus solitarius GP-COCCUS C- Y BUG+B Air 35-37 

158 Enterococcus sulfureus GP-COCCUS C- Y BUG+B Air 35-37 

159 Eremococcus coleocola GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

160 Erysipelothrix rhusiopathiae/tonsillarum GP-ROD C- Y BUG+B 6.5%CO2 35-37 

161 Exiguobacterium acetylicum GP-ROD C+ Y BUG+B Air 30 

162 Gardnerella vaginalis GP-ROD C- Y BUG+B 6.5%CO2 35-37 

163 Gemella bergeri GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

164 Gemella haemolysans/morbillorum GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

165 Gemella palaticanis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

166 Gemella sanguinis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

167 Geobacillus stearothermophilus GP-ROD SB C-/W+ N BUG+M Air 55 

168 Geobacillus thermoglucosidasius GP-ROD SB C-/W+ N BUG+M Air 55 

169 Globicatella sanguinis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

170 Gordonia aichiensis GP-ROD C+ Y BUG+B Air 35-37 

171 Gordonia bronchialis GP-ROD C+ Y BUG+B Air 35-37 

172 Gordonia rubroprincta GP-ROD C+ Y BUG+B Air 35-37 

173 Gordonia sputi GP-ROD C+ Y BUG+B Air 35-37 

174 Gordonia terrae GP-ROD C+ Y BUG+B Air 35-37 

175 Helcococcus kunzii GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

176 Ignavigranum rouffiae GP-COCCUS C+/- Y BUG+B 6.5%CO2 35-37 

177 Jonesia denitrificans GP-ROD C+ Y BUG+B Air 35-37 

178 Kocuria kristinae GP-COCCUS C+ Y BUG+B Air 30 

179 Kocuria rosea GP-COCCUS C+ Y BUG+B Air 30 

180 Kocuria varians GP-COCCUS C+ Y BUG+B Air 30 

181 Kurthia gibsonii GP-ROD C+ Y BUG+B Air 30 

182 Kurthia sibirica GP-ROD C+ Y BUG+B Air 30 

183 Kurthia zopfii GP-ROD C+ Y BUG+B Air 35-37 

184 Kytococcus sedentarius GP-COCCUS C+ Y BUG+B Air 30 

185 Lactococcus garvieae GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

186 Lactococcus lactis ss cremoris GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

187 Lactococcus lactis ss diacetylactis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

188 Lactococcus lactis ss hordniae GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

189 Lactococcus lactis ss lactis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

190 Lactococcus plantarum GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

191 Lactococcus raffinolactis GP-COCCUS C- Y BUG+B Air 30 

192 Leifsonia aquatica GP-ROD C+ Y BUG+B Air 35-37 

193 Leuconostoc carnosum GP-COCCUS C- Y BUG+B Air 30 

194 Leuconostoc citreum GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

195 Leuconostoc fallax GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

196 Leuconostoc gelidum GP-COCCUS C- Y BUG+B Air 30 

197 Leuconostoc lactis GP-COCCUS C- Y BUG+B Air 30 

198 Leuconostoc mesenteroides GP-COCCUS C- Y BUG+B Air 30 

199 Leuconostoc mesenteroides ss dextranicum GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

200 Leuconostoc mesenteroides ss mesenteroides GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 




201 Listeria grayi GP-ROD C+ Y BUG+B Air 35-37 

202 Listeria innocua/monocytogenes/seeligeri/welshimeri GP-ROD C+ Y BUG+B Air 35-37 

203 Listeria ivanovii GP-ROD C+ Y BUG+B Air 35-37 

204 Listeria ivanovii ss londoniensis GP-ROD C+ Y BUG+B Air 35-37 

205 Listeria monocytogenes/innocua/seeligeri/welshimeri GP-ROD C+ Y BUG+B Air 35-37 

206 Listeria seeligeri/innocua/monocytogenes/welshimeri GP-ROD C+ Y BUG+B Air 35-37 

207 Listeria welshimeri/innocua/monocytogenes/seeligeri GP-ROD C+ Y BUG+B Air 35-37 

208 Macrococcus bovicus GP-COCCUS C+ Y BUG+B Air 35-37 

209 Macrococcus carouselicus GP-COCCUS C+ Y BUG+B Air 35-37 

210 Macrococcus caseolyticus GP-COCCUS C+ Y BUG+B Air 35-37 

211 Macrococcus equipercicus GP-COCCUS C+ Y BUG+B Air 35-37 

212 Microbacterium arborescens GP-ROD C+/- Y BUG+B Air 35-37 

213 Microbacterium esteraromaticum GP-ROD C+/- Y BUG+B Air 35-37 

214 Microbacterium flavescens GP-ROD C+/- Y BUG+B Air 35-37 

215 Microbacterium imperiale GP-ROD C+/- Y BUG+B Air 35-37 

216 Microbacterium lacticum GP-ROD C+/- Y BUG+B Air 35-37 

217 Microbacterium laevaniformans GP-ROD C+/- Y BUG+B Air 35-37 

218 Microbacterium liquefaciens GP-ROD C+/- Y BUG+B Air 35-37 

219 Microbacterium maritypicum GP-ROD C+/- Y BUG+B Air 30 

220 Microbacterium saperdae GP-ROD C+/- Y BUG+B Air 30 

221 Microbacterium spp. (CDC.A-4) GP-ROD C+/- Y BUG+B Air 35-37 

222 Microbacterium spp. (CDC.A-5) GP-ROD C+/- Y BUG+B Air 35-37 

223 Microbacterium terregens GP-ROD C+/- Y BUG+B Air 35-37 

224 Microbacterium testaceum GP-ROD C+/- Y BUG+B Air 35-37 

225 "Micrococcus diversus" GP-COCCUS C+ Y BUG+B Air 30 

226 Micrococcus luteus GP-COCCUS C+ Y BUG+B Air 30 

227 Micrococcus luteus (ATCC 9341) GP-COCCUS C+ Y BUG+B Air 30 

228 Micrococcus lylae GP-COCCUS C+ Y BUG+B Air 30 

229 Paenibacillus azotofixans GP-ROD SB C- N BUG+M Air 30 

230 Paenibacillus larvae ss larvae GP-ROD SB C-/W+ N BUG+M Air 30 

231 Paenibacillus macerans GP-ROD SB C-/W+ N BUG+M Air 30 

232 Paenibacillus pabuli GP-ROD SB C-/W+ N BUG+M Air 30 

233 Paenibacillus polymyxa GP-ROD SB C-/W+ N BUG+M Air 30 

234 Paenibacillus popilliae GP-ROD SB C- N BUG+M Air 30 

235 Paenibacillus validus GP-ROD SB C-/W+ N BUG+M Air 30 

236 Pediococcus acidilactici/parvulus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

237 Pediococcus dextrinicus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

238 Pediococcus pentosaceus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

239 Pediococcus urinaeequi GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

240 Pediococcus urinaeequi-like GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

241 Rathayibacter rathayi GP-ROD C+ N BUG Air 30 

242 Rathayibacter tritici GP-ROD C+ N BUG Air 30 

243 Rhodococcus australis GP-ROD C+ Y BUG+B Air 35-37 

244 Rhodococcus coprophilus GP-ROD C+ Y BUG+B Air 35-37 

245 Rhodococcus equi GP-ROD C+ Y BUG+B Air 35-37 

246 Rhodococcus erythropolis GP-ROD C+ Y BUG+B Air 35-37 

247 Rhodococcus fascians GP-ROD C+ Y BUG+B Air 35-37 

248 Rhodococcus globerulus GP-ROD C+ Y BUG+B Air 35-37 

249 Rhodococcus rhodnii GP-ROD C+ Y BUG+B Air 35-37 

250 Rhodococcus rhodochrous GP-ROD C+ Y BUG+B Air 35-37 

251 Rhodococcus ruber GP-ROD C+ Y BUG+B Air 35-37 

252 Rothia dentocariosa GP-ROD C+/- Y BUG+B Air 35-37 

253 Rothia mucilaginosa GP-COCCUS C-/W+ Y BUG+B Air 35-37 

254 Sanguibacter inulinus GP-ROD C+ Y BUG+B 6.5%CO2 35-37 

255 Sanguibacter keddieii GP-ROD C+ Y BUG+B Air 30 

256 Sanguibacter suarezii GP-ROD C+ Y BUG+B Air 30 

257 Staphylococcus arlettae GP-COCCUS C+ Y BUG+B Air 35-37 

258 Staphylococcus aureus ss anaerobius GP-COCCUS C- Y BUG+B Air 35-37 

259 Staphylococcus aureus ss aureus GP-COCCUS C+ Y BUG+B Air 35-37 

260 Staphylococcus auricularis GP-COCCUS C+ Y BUG+B Air 35-37 

261 Staphylococcus capitis GP-COCCUS C+ Y BUG+B Air 35-37 

262 Staphylococcus caprae GP-COCCUS C+ Y BUG+B Air 35-37 

263 Staphylococcus carnosus GP-COCCUS C+ Y BUG+B Air 35-37 

264 Staphylococcus chromogenes GP-COCCUS C+ Y BUG+B Air 35-37 

265 Staphylococcus cohnii GP-COCCUS C+ Y BUG+B Air 35-37 

266 Staphylococcus delphini GP-COCCUS C+ Y BUG+B Air 35-37 

267 Staphylococcus epidermidis GP-COCCUS C+ Y BUG+B Air 35-37 

268 Staphylococcus equorum GP-COCCUS C+ Y BUG+B Air 35-37 








269 Staphylococcus felis GP-COCCUS C+ Y BUG+B Air 35-37 

270 Staphylococcus gallinarum GP-COCCUS C+ Y BUG+B Air 35-37 

271 Staphylococcus haemolyticus GP-COCCUS C+ Y BUG+B Air 35-37 

272 Staphylococcus hominis GP-COCCUS C+ Y BUG+B Air 35-37 

273 Staphylococcus hominis ss novobiosepticus GP-COCCUS C+ Y BUG+B Air 35-37 

274 Staphylococcus hyicus GP-COCCUS C+ Y BUG+B Air 35-37 

275 Staphylococcus intermedius GP-COCCUS C+ Y BUG+B Air 35-37 

276 Staphylococcus kloosii GP-COCCUS C+ Y BUG+B Air 35-37 

277 Staphylococcus lentus GP-COCCUS C+ Y BUG+B Air 35-37 

278 Staphylococcus lugdunensis GP-COCCUS C+ Y BUG+B Air 35-37 

279 Staphylococcus lutrae GP-COCCUS C+ Y BUG+B Air 35-37 

280 Staphylococcus muscae GP-COCCUS C+ Y BUG+B Air 35-37 

281 Staphyloccocus pasteuri GP-COCCUS C+ Y BUG+B Air 35-37 

282 Staphylococcus piscifermentans GP-COCCUS C+ Y BUG+B Air 35-37 

283 Staphylococcus pulvereri/vitulinus GP-COCCUS C+ Y BUG+B Air 35-37 

284 Staphylococcus saprophyticus GP-COCCUS C+ Y BUG+B Air 35-37 

285 Staphylococcus schleiferi GP-COCCUS C+ Y BUG+B Air 35-37 

286 Staphylococcus sciuri GP-COCCUS C+ Y BUG+B Air 35-37 

287 Staphylococcus sciuri ss rodentium GP-COCCUS C+ Y BUG+B Air 35-37 

288 Staphylococcus simulans GP-COCCUS C+ Y BUG+B Air 35-37 

289 Staphylococcus warneri GP-COCCUS C+ Y BUG+B Air 35-37 

290 Staphylococcus xylosus GP-COCCUS C+ Y BUG+B Air 35-37 

291 Streptococcus acidominimus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

292 Streptococcus agalactiae A (GP B) GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

293 Streptococcus agalactiae B (GP B) GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

294 Streptococcus alactolyticus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

295 Streptococcus anginosus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

296 Streptococcus bovis (GP D) GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

297 Streptococcus canis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

298 Streptococcus constellatus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

299 Streptococcus criceti GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

300 Streptococcus cristatus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

301 Streptococcus downei GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

302 Streptococcus dysgalactiae ss dysgalactiae GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

303 Streptococcus dysgalactiae ss equisimilis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

304 Streptococcus equi ss equi GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

305 Streptococcus equi ss zooepidemicus (GP C) GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

306 Streptococcus equinus (GP D) GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

307 Streptococcus ferus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

308 Streptococcus gallolyticus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

309 Streptococcus gordonii GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

310 Streptococcus hyointestinalis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

311 Streptococcus hyovaginalis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

312 Streptococcus infantarius ss coli GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

313 Streptococcus infantarius ss infantarius GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

314 Streptococcus infantis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

315 Streptococcus iniae GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

316 Streptococcus intermedius GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

317 Streptococcus intestinalis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

318 Streptococcus macacae GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

319 Streptococcus macedonicus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

320 Streptococcus mitis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

321 Streptococcus mutans/ratti GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

322 Streptococcus oralis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

323 Streptococcus parasanguinis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

324 Streptococcus peroris GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

325 Streptococcus phocae GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

326 Streptococcus pluranimalium GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

327 Streptococcus pneumoniae GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

328 Streptococcus porcinus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

329 Streptococcus pyogenes A (GP A) GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

330 Streptococcus pyogenes B (GP A) GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

331 Streptococcus pyogenes C (GP A) GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

332 Streptococcus salivarius GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

333 Streptococcus sanguinis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

334 Streptococcus sobrinus GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

335 Streptococcus suis (GP RST) GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

336 Streptococcus suis serogroup 1/2 GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 




337 Streptococcus suis serogroup 4/6 GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

338 Streptococcus suis serogroup 5 GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

339 Streptococcus suis serogroup 7 GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

340 Streptococcus thoraltensis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

341 Streptococcus uberis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

342 Streptococcus vestibularis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

343 Streptococcus waius GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

344 Tetragenococcus halophilus GP-COCCUS C- Y BUG+B Air 30 

345 Tsukamurella inchonensis GP-ROD C+ Y BUG+B Air 35-37 

346 Tsukamurella paurometabola GP-ROD C+ Y BUG+B Air 35-37 

347 Turicella otitidis GP-ROD C+ Y BUG+B Air 35-37 

348 Vagococcus fluvialis GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

349 Vagococcus lutrae GP-COCCUS C- Y BUG+B 6.5%CO2 35-37 

350 Vagococcus salmoninarum GP-COCCUS C- Y BUG+B Air 26 

351 Virgibacillus pantothenticus GP-ROD SB C-/W+ N BUG+M Air 30 





*Indicates species that require a MicroStation Reader for identification. 



1 Abiotrophia defectiva AN GP-COCCUS N/A N BUA ANA 35-37 

2 Acetivibrio ethanolgignens AN GN-ROD N/A N BUA ANA 35-37 

3 Acetoanaerobium noterae AN GN-ROD N/A N BUA ANA 35-37 

4 Acidaminococcus fermentans AN GN-COCCUS N/A N BUA ANA 35-37 

5 Actinobaculum schaalii* AN GP-ROD N/A N BUA ANA 35-37 

6 Actinobaculum suis* AN GP-ROD N/A N BUA ANA 35-37 

7 Actinomyces bovis BGA* AN GP-ROD N/A N BUA ANA 35-37 

8 Actinomyces bovis BGB* AN GP-ROD N/A N BUA ANA 35-37 

9 Actinomyces europaeus* AN GP-ROD N/A N BUA ANA 35-37 

10 Actinomyces graevenitzii* AN GP-ROD N/A N BUA ANA 35-37 

11 Actinomyces howellii* AN GP-ROD N/A N BUA ANA 35-37 

12 Actinomyces hyovaginalis* AN GP-ROD N/A N BUA ANA 35-37 

13 Actinomyces israelii* AN GP-ROD N/A N BUA ANA 35-37 

14 Actinomyces meyeri* AN GP-ROD N/A N BUA ANA 35-37 

15 Actinomyces naeslundii/viscosus* AN GP-ROD N/A N BUA ANA 35-37 

16 Actinomyces neuii* AN GP-ROD N/A N BUA ANA 35-37 

17 Actinomyces odontolyticus* AN GP-ROD N/A N BUA ANA 35-37 

18 Actinomyces radingae* AN GP-ROD N/A N BUA ANA 35-37 

19 Actinomyces slackii* AN GP-ROD N/A N BUA ANA 35-37 

20 Actinomyces turicensis* AN GP-ROD N/A N BUA ANA 35-3 

21 Anaerobiospirillum succiniproducens AN GN-ROD N/A N BUA ANA 35-37 

22 Anaeromusa acidaminophila AN GN-ROD N/A N BUA ANA 35-37 

23 Anaerorhabdus furcosa AN GN-ROD N/A N BUA ANA 35-37 

24 Anaerosinus glycerini AN GN-ROD N/A N BUA ANA 35-37 

25 Arcanobacterium pyogenes* AN GP-ROD N/A N BUA ANA 35-37 

26 Atopobium fossor AN GP-ROD N/A N BUA ANA 35-37 

27 Atopobium minutum AN GP-COCCUS N/A N BUA ANA 35-37 

28 Atopobium parvulum AN GP-COCCUS N/A N BUA ANA 35-37 

29 Bacteroides caccae* AN GN-ROD N/A N BUA ANA 35-37 

30 Bacteroides coagulans* AN GN-ROD N/A N BUA ANA 35-37 

31 Bacteroides distasonis* AN GN-ROD N/A N BUA ANA 35-37 

32 Bacteroides eggerthii* AN GN-ROD N/A N BUA ANA 35-37 

33 Bacteroides forsythus* AN GN-ROD N/A N BUA ANA 35-37 

34 Bacteroides fragilis BGA* AN GN-ROD N/A N BUA ANA 35-37 

35 Bacteroides fragilis BGB* AN GN-ROD N/A N BUA ANA 35-37 

36 Bacteroides helcogenes* AN GN-ROD N/A N BUA ANA 35-37 

37 Bacteroides merdae* AN GN-ROD N/A N BUA ANA 35-37 

38 Bacteroides ovatus* AN GN-ROD N/A N BUA ANA 35-37 

39 Bacteroides pectinophilus* AN GN-ROD N/A N BUA ANA 35-37 

40 Bacteroides putredinis* AN GN-ROD N/A N BUA ANA 35-37 

41 Bacteroides pyogenes* AN GN-ROD N/A N BUA ANA 35-37 

42 Bacteroides splanchnicus* AN GN-ROD N/A N BUA ANA 35-37 

43 Bacteroides stercoris* AN GN-ROD N/A N BUA ANA 35-37 

44 Bacteroides suis* AN GN-ROD N/A N BUA ANA 35-37 

45 Bacteroides tectus* AN GN-ROD N/A N BUA ANA 35-37 

46 Bacteroides thetaiotaomicron* AN GN-ROD N/A N BUA ANA 35-37 

47 Bacteroides uniformis* AN GN-ROD N/A N BUA ANA 35-37 

48 Bacteroides ureolyticus* AN GN-ROD N/A N BUA ANA 35-37 

49 Bacteroides vulgatus* AN GN-ROD N/A N BUA ANA 35-37 

50 Bifidobacterium adolescentis AN GP-ROD N/A N BUA ANA 35-37 

51 Bifidobacterium angulatum AN GP-ROD N/A N BUA ANA 35-37 

52 Bifidobacterium animalis AN GP-ROD N/A N BUA ANA 35-37 

53 Bifidobacterium asteroides AN GP-ROD N/A N BUA ANA 35-37 

54 Bifidobacterium bifidum AN GP-ROD N/A N BUA ANA 35-37 

55 Bifidobacterium boum AN GP-ROD N/A N BUA ANA 35-37 

56 Bifidobacterium breve AN GP-ROD N/A N BUA ANA 35-37 

57 Bifidobacterium catenulatum AN GP-ROD N/A N BUA ANA 35-37 

58 Bifidobacterium choerinum AN GP-ROD N/A N BUA ANA 35-37 

59 Bifidobacterium coryneforme AN GP-ROD N/A N BUA ANA 35-37 

60 Bifidobacterium cuniculi AN GP-ROD N/A N BUA ANA 35-37 

61 Bifidobacterium dentium AN GP-ROD N/A N BUA ANA 35-37 

62 Bifidobacterium gallicum AN GP-ROD N/A N BUA ANA 35-37 

63 Bifidobacterium gallinarum AN GP-ROD N/A N BUA ANA 35-37 

64 Bifidobacterium indicum AN GP-ROD N/A N BUA ANA 35-37 

65 Bifidobacterium infantis AN GP-ROD N/A N BUA ANA 35-37 

66 Bifidobacterium longum AN GP-ROD N/A N BUA ANA 35-37 

MicroStation System/MicroLog User Guide Apr 07 Section 13 � Page- 19 -



67 Bifidobacterium magnum AN GP-ROD N/A N BUA ANA 35-37 

68 Bifidobacterium merycicum AN GP-ROD N/A N BUA ANA 35-37 

69 Bifidobacterium minimum AN GP-ROD N/A N BUA ANA 35-37 

70 Bifidobacterium pseudocatenulatum AN GP-ROD N/A N BUA ANA 35-37 

71 Bifidobacterium pseudolongum ss globosum AN GP-ROD N/A N BUA ANA 35-37 

72 Bifidobacterium pseudolongum ss pseudolongum AN GP-ROD N/A N BUA ANA 35-37 

73 Bifidobacterium pullorum AN GP-ROD N/A N BUA ANA 35-37 

74 Bifidobacterium ruminantium AN GP-ROD N/A N BUA ANA 35-37 

75 Bifidobacterium saeculare AN GP-ROD N/A N BUA ANA 35-37 

76 Bifidobacterium subtile AN GP-ROD N/A N BUA ANA 35-37 

77 Bifidobacterium suis AN GP-ROD N/A N BUA ANA 35-37 

78 Bifidobacterium thermophilum AN GP-ROD N/A N BUA ANA 35-37 

79 Bilophila wadsworthia AN GN-ROD N/A N BUA ANA 35-37 

80 Butyrivibrio fibrisolvens* AN GP-ROD N/A N BUA ANA 35-37 

81 Campylobacter curvus/gracilis AN GN-ROD N/A N BUA ANA 35-37 

82 Campylobacter rectus AN GN-ROD N/A N BUA ANA 35-37 

83 Catonella morbi AN GN-ROD N/A N BUA ANA 35-37 

84 Centipeda periodontii AN GN-ROD N/A N BUA ANA 35-37 

85 Clostridium absonum AN GP-ROD N/A N BUA ANA 35-37 

86 Clostridium acetobutylicum AN GP-ROD N/A N BUA ANA 35-37 

87 Clostridium aerotolerans AN GP-ROD N/A N BUA ANA 35-37 

88 Clostridium “aminobutyricum” AN GP-ROD N/A N BUA ANA 35-37 

89 Clostridium aminophilum AN GP-ROD N/A N BUA ANA 35-37 

90 Clostridium aminovalericum AN GP-ROD N/A N BUA ANA 35-37 

91 Clostridium barati AN GP-ROD N/A N BUA ANA 35-37 

92 Clostridium botulinum AN GP-ROD N/A N BUA ANA 35-37 

93 Clostridium cadaveris AN GP-ROD N/A N BUA ANA 35-37 

94 Clostridium carnis AN GP-ROD N/A N BUA ANA 35-37 

95 Clostridium cellobioparum AN GP-ROD N/A N BUA ANA 35-37 

96 Clostridium cellulolyticum AN GP-ROD N/A N BUA ANA 35-37 

97 Clostridium clostridiiforme AN GP-ROD N/A N BUA ANA 35-37 

98 Clostridium coccoides AN GP-ROD N/A N BUA ANA 35-37 

99 Clostridium cochlearium AN GP-ROD N/A N BUA ANA 35-37 

100 Clostridium cocleatum AN GP-ROD N/A N BUA ANA 35-37 

101 Clostridium colinum AN GP-ROD N/A N BUA ANA 35-37 

102 Clostridium cylindrosporum AN GP-ROD N/A N BUA ANA 35-37 

103 Clostridium difficile AN GP-ROD N/A N BUA ANA 35-37 

104 Clostridium fallax AN GP-ROD N/A N BUA ANA 35-37 

105 Clostridium glycolicum AN GP-ROD N/A N BUA ANA 35-37 

106 Clostridium hastiforme AN GP-ROD N/A N BUA ANA 35-37 

107 Clostridium hydroxybenzoicum AN GP-ROD N/A N BUA ANA 35-37 

108 Clostridium innocuum AN GP-ROD N/A N BUA ANA 35-37 

109 Clostridium intestinale AN GP-ROD N/A N BUA ANA 35-37 

110 Clostridium irregularis AN GP-ROD N/A N BUA ANA 35-37 

111 Clostridium magnum AN GP-ROD N/A N BUA ANA 35-37 

112 Clostridium malenominatum AN GP-ROD N/A N BUA ANA 35-37 

113 Clostridium nexile AN GP-ROD N/A N BUA ANA 35-37 

114 Clostridium novyi AN GP-ROD N/A N BUA ANA 35-37 

115 Clostridium orbiscindens AN GP-ROD N/A N BUA ANA 35-37 

116 Clostridium oroticum AN GP-ROD N/A N BUA ANA 35-37 

117 Clostridium papyrosolvens AN GP-ROD N/A N BUA ANA 35-37 

118 Clostridium paraputrificum AN GP-ROD N/A N BUA ANA 35-37 

119 Clostridium pasteurianum AN GP-ROD N/A N BUA ANA 35-37 

120 Clostridium perfringens AN GP-ROD N/A N BUA ANA 35-37 

121 Clostridium propionicum AN GP-ROD N/A N BUA ANA 35-37 

122 Clostridium purinolyticum AN GP-ROD N/A N BUA ANA 35-37 

123 Clostridium putrificum AN GP-ROD N/A N BUA ANA 35-37 

124 Clostridium ramosum AN GP-ROD N/A N BUA ANA 35-37 

125 Clostridium rectum AN GP-ROD N/A N BUA ANA 35-37 

126 Clostridium saccharolyticum AN GP-ROD N/A N BUA ANA 35-37 

127 Clostridium sardiniensis AN GP-ROD N/A N BUA ANA 35-37 

128 Clostridium sartagoformum AN GP-ROD N/A N BUA ANA 35-37 

129 Clostridium scatologenes AN GP-ROD N/A N BUA ANA 35-37 

130 Clostridium scindens AN GP-ROD N/A N BUA ANA 35-37 

131 Clostridium septicum AN GP-ROD N/A N BUA ANA 35-37 

132 Clostridium sordelli AN GP-ROD N/A N BUA ANA 35-37 

133 Clostridium sphenoides AN GP-ROD N/A N BUA ANA 35-37 

134 Clostridium spiroforme AN GP-ROD N/A N BUA ANA 35-37 

135 Clostridium sporogenes AN GP-ROD N/A N BUA ANA 35-37 


Section 13 � Page - 20 - MicroStation System/MicroLog User Guide Apr 07




136 Clostridium sporosphaeroides AN GP-ROD N/A N BUA ANA 35-37 

137 Clostridium sticklandii AN GP-ROD N/A N BUA ANA 35-37 

138 Clostridium subterminale AN GP-ROD N/A N BUA ANA 35-37 

139 Clostridium symbiosum AN GP-ROD N/A N BUA ANA 35-37 

140 Clostridium tertium AN GP-ROD N/A N BUA ANA 35-37 

141 Clostridium tetani AN GP-ROD N/A N BUA ANA 35-37 

142 Clostridium tetanomorphum AN GP-ROD N/A N BUA ANA 35-37 

143 Clostridium tyrobutyricum AN GP-ROD N/A N BUA ANA 35-37 

144 Clostridium/Eubacterium species AN GP-ROD N/A N BUA ANA 35-37 

145 Collinsella aerofaciens AN GP-ROD N/A N BUA ANA 35-37 

146 Coprococcus catus AN GP-COCCUS N/A N BUA ANA 35-37 

147 Dendrosporobacter quercicolus AN GP-ROD N/A N BUA ANA 35-37 

148 Desulfobulbus elongatus AN GN-ROD N/A N BUA ANA 35-37 

149 Desulfomonas pigra AN GN-ROD N/A N BUA ANA 35-37 

150 Desulfomonile tiedjei AN GN-ROD N/A N BUA ANA 35-37 

151 Desulfovibrio desulfuricans ss desulfuricans AN GN-ROD N/A N BUA ANA 35-37 

152 Desulfovibrio fructosivorans AN GN-ROD N/A N BUA ANA 35-37 

153 Desulfovibrio vulgaris ss vulgaris AN GN-ROD N/A N BUA ANA 35-37 

154 Eggerthella lenta* AN GP-ROD N/A N BUA ANA 35-37 

155 Eikenella corrodens* AN GN-ROD N/A N BUA ANA 35-37 

156 Eubacterium angustum AN GP-ROD N/A N BUA ANA 35-37 

157 Eubacterium barkeri AN GP-ROD N/A N BUA ANA 35-37 

158 Eubacterium biforme AN GP-ROD N/A N BUA ANA 35-37 

159 Eubacterium brachy AN GP-ROD N/A N BUA ANA 35-37 

160 Eubacterium budayi AN GP-ROD N/A N BUA ANA 35-37 

161 Eubacterium combesii AN GP-ROD N/A N BUA ANA 35-37 

162 Eubacterium contortum AN GP-ROD N/A N BUA ANA 35-37 

163 Eubacterium cylindroides AN GP-ROD N/A N BUA ANA 35-37 

164 Eubacterium desmolans AN GP-ROD N/A N BUA ANA 35-37 

165 Eubacterium dolichum AN GP-ROD N/A N BUA ANA 35-37 

166 Eubacterium fissicatena AN GP-ROD N/A N BUA ANA 35-37 

167 Eubacterium hallii AN GP-ROD N/A N BUA ANA 35-37 

168 Eubacterium limosum BGA AN GP-ROD N/A N BUA ANA 35-37 

169 Eubacterium limosum BGB AN GP-ROD N/A N BUA ANA 35-37 

170 Eubacterium moniliforme AN GP-ROD N/A N BUA ANA 35-37 

171 Eubacterium multiforme AN GP-ROD N/A N BUA ANA 35-37 

172 Eubacterium nodatum AN GP-ROD N/A N BUA ANA 35-37 

173 Eubacterium plautii AN GP-ROD N/A N BUA ANA 35-37 

174 Eubacterium saburreum AN GP-ROD N/A N BUA ANA 35-37 

175 Eubacterium tortuosum AN GP-ROD N/A N BUA ANA 35-37 

176 Eubacterium uniforme AN GP-ROD N/A N BUA ANA 35-37 

177 Eubacterium yurii ss margaretiae AN GP-ROD N/A N BUA ANA 35-37 

178 Eubacterium yurii ss schtitka AN GP-ROD N/A N BUA ANA 35-37 

179 Eubacterium yurii ss yurii AN GP-ROD N/A N BUA ANA 35-37 

180 Facklamia hominis AN GP-COCCUS N/A N BUA ANA 35-37 

181 Facklamia sourekii AN GP-COCCUS N/A N BUA ANA 35-37 

182 Falcivibrio grandis AN GP-ROD N/A N BUA ANA 35-37 

183 Falcivibrio vaginalis AN GP-ROD N/A N BUA ANA 35-37 

184 Filifactor alocis* AN GN-ROD N/A N BUA ANA 35-37 

185 Filifactor villosus AN GP-ROD N/A N BUA ANA 35-37 

186 Finegoldia magna AN GP-COCCUS N/A N BUA ANA 35-37 

187 Fusobacterium gonidiaformans* AN GN-ROD N/A N BUA ANA 35-37 

188 Fusobacterium naviforme* AN GN-ROD N/A N BUA ANA 35-37 

189 Fusobacterium necrogenes* AN GN-ROD N/A N BUA ANA 35-37 

190 Fusobacterium necrophorum* AN GN-ROD N/A N BUA ANA 35-37 

191 Fusobacterium nucleatum ss animalis* AN GN-ROD N/A N BUA ANA 35-37 

192 Fusobacterium nucleatum ss fusiforme* AN GN-ROD N/A N BUA ANA 35-37 

193 Fusobacterium nucleatum ss nucleatum* AN GN-ROD N/A N BUA ANA 35-37 

194 Fusobacterium nucleatum ss polymorphum* AN GN-ROD N/A N BUA ANA 35-37 

195 Fusobacterium nucleatum ss vincentii* AN GN-ROD N/A N BUA ANA 35-37 

196 Fusobacterium russii* AN GN-ROD N/A N BUA ANA 35-37 

197 Fusobacterium simiae* AN GN-ROD N/A N BUA ANA 35-37 

198 Fusobacterium ulcerans* AN GN-ROD N/A N BUA ANA 35-37 

199 Fusobacterium varium* AN GN-ROD N/A N BUA ANA 35-37 

200 Gardnerella vaginalis AN GP-ROD N/A N BUA ANA 35-37 

201 Gemella bergeri AN GP-COCCUS N/A N BUA ANA 35-37 

202 Gemella haemolysans AN GP-COCCUS N/A N BUA ANA 35-37 

203 Gemella morbillorum AN GP-COCCUS N/A N BUA ANA 35-37 

204 Granulicatella adiacens AN GP-COCCUS N/A N BUA ANA 35-37 




205 Hallella seregens AN GN-ROD N/A N BUA ANA 35-37 

206 Lactobacillus acetotolerans AN GP-ROD N/A N BUA ANA 35-37 

207 Lactobacillus acidophilus BGA AN GP-ROD N/A N BUA ANA 35-37 

208 Lactobacillus acidophilus BGB AN GP-ROD N/A N BUA ANA 35-37 

209 Lactobacillus alimentarius AN GP-ROD N/A N BUA ANA 35-37 

210 Lactobacillus amylovorus AN GP-ROD N/A N BUA ANA 35-37 

211 Lactobacillus aviarius ss araffinosus AN GP-ROD N/A N BUA ANA 35-37 

212 Lactobacillus aviarius ss aviarius AN GP-ROD N/A N BUA ANA 35-37 

213 Lactobacillus bifermentans AN GP-ROD N/A N BUA ANA 35-37 

214 Lactobacillus brevis AN GP-ROD N/A N BUA ANA 35-37 

215 Lactobacillus buchneri AN GP-ROD N/A N BUA ANA 35-37 

216 Lactobacillus casei AN GP-ROD N/A N BUA ANA 35-37 

217 Lactobacillus catenaformis AN GP-ROD N/A N BUA ANA 35-37 

218 Lactobacillus collinoides AN GP-ROD N/A N BUA ANA 26 

219 Lactobacillus coryniformis ss coryniformis AN GP-ROD N/A N BUA ANA 35-37 

220 Lactobacillus coryniformis ss torquens AN GP-ROD N/A N BUA ANA 35-37 

221 Lactobacillus crispatus AN GP-ROD N/A N BUA ANA 35-37 

222 Lactobacillus curvatus AN GP-ROD N/A N BUA ANA 35-37 

223 Lactobacillus delbrueckii ss bulgaricus AN GP-ROD N/A N BUA ANA 35-37 

224 Lactobacillus delbrueckii ss delbrueckii AN GP-ROD N/A N BUA ANA 35-37 

225 Lactobacillus delbrueckii ss lactis AN GP-ROD N/A N BUA ANA 35-37 

226 Lactobacillus farciminis AN GP-ROD N/A N BUA ANA 35-37 

227 Lactobacillus fermentum AN GP-ROD N/A N BUA ANA 35-37 

228 Lactobacillus fructivorans AN GP-ROD N/A N BUA ANA 35-37 

229 Lactobacillus fructosus AN GP-ROD N/A N BUA ANA 35-37 

230 Lactobacillus gasseri AN GP-ROD N/A N BUA ANA 35-37 

231 Lactobacillus hamsteri AN GP-ROD N/A N BUA ANA 35-37 

232 Lactobacillus helveticus AN GP-ROD N/A N BUA ANA 35-37 

233 Lactobacillus hilgardii AN GP-ROD N/A N BUA ANA 26 

234 Lactobacillus jensenii AN GP-ROD N/A N BUA ANA 35-37 

235 Lactobacillus kefiri AN GP-ROD N/A N BUA ANA 35-37 

236 Lactobacillus malefermentans AN GP-ROD N/A N BUA ANA 30 

237 Lactobacillus mali/sakei ss sakei AN GP-ROD N/A N BUA ANA 35-37 

238 Lactobacillus murinus/paracasei ss tolerans AN GP-ROD N/A N BUA ANA 35-37 

239 Lactobacillus oris/parabuchneri AN GP-ROD N/A N BUA ANA 35-37 

240 Lactobacillus paracasei ss paracasei AN GP-ROD N/A N BUA ANA 35-37 

241 Lactobacillus pentosus AN GP-ROD N/A N BUA ANA 35-37 

242 Lactobacillus plantarum AN GP-ROD N/A N BUA ANA 35-37 

243 Lactobacillus reuteri AN GP-ROD N/A N BUA ANA 35-37 

244 Lactobacillus rhamnosus AN GP-ROD N/A N BUA ANA 35-37 

245 Lactobacillus salivarius ss salicinius AN GP-ROD N/A N BUA ANA 35-37 

246 Lactobacillus salivarius ss salivarius AN GP-ROD N/A N BUA ANA 35-37 

247 Lactobacillus sanfranciscensis AN GP-ROD N/A N BUA ANA 35-37 

248 Lactobacillus suebicus AN GP-ROD N/A N BUA ANA 35-37 

249 Lactobacillus vaginalis AN GP-ROD N/A N BUA ANA 35-37 

250 Lactococcus garvieae AN GP-COCCUS N/A N BUA ANA 35-37 

251 Lactococcus lactis ss cremoris AN GP-COCCUS N/A N BUA ANA 35-37 

252 Lactococcus lactis ss hordniae AN GP-COCCUS N/A N BUA ANA 35-37 

253 Lactococcus lactis ss lactis AN GP-COCCUS N/A N BUA ANA 35-37 

254 Lactococcus plantarum AN GP-COCCUS N/A N BUA ANA 35-37 

255 Lactococcus raffinolactis AN GP-COCCUS N/A N BUA ANA 35-37 

256 Leuconostoc carnosum AN GP-COCCUS N/A N BUA ANA 35-37 

257 Leuconostoc citreum AN GP-COCCUS N/A N BUA ANA 35-37 

258 Leuconostoc fallax AN GP-COCCUS N/A N BUA ANA 35-37 

259 Leuconostoc gelidum AN GP-COCCUS N/A N BUA ANA 26 

260 Leuconostoc lactis AN GP-COCCUS N/A N BUA ANA 35-37 

261 Leuconostoc mesenteroides ss cremoris AN GP-COCCUS N/A N BUA ANA 35-37 

262 Leuconostoc mesenteroides ss dextranicum AN GP-COCCUS N/A N BUA ANA 35-37 

263 Leuconostoc mesenteroides ss mesenteroides AN GP-COCCUS N/A N BUA ANA 35-37 

264 Megamonas hypermegale AN GN-ROD N/A N BUA ANA 35-37 

265 Megasphaera cerevisiae AN GN-COCCUS N/A N BUA ANA 35-37 

266 Megasphaera elsdenii AN GN-COCCUS N/A N BUA ANA 35-37 

267 Micromonas micros AN GP-COCCUS N/A N BUA ANA 35-37 

268 Mobiluncus curtisii AN GP-ROD N/A N BUA ANA 35-37 

269 Mobiluncus mulieris AN GP-ROD N/A N BUA ANA 35-37 

270 Mongibacterium timidum AN GP-ROD N/A N BUA ANA 35-37 

271 Pectinatus cerevisiiphilus AN GN-ROD N/A N BUA ANA 30 

272 Pectinatus frisingensis AN GN-ROD N/A N BUA ANA 30 

273 Pediococcus acidilactici AN GP-COCCUS N/A N BUA ANA 35-37 






274 Pediococcus dextrinicus AN GP-COCCUS N/A N BUA ANA 35-37 

275 Pediococcus parvulus AN GP-COCCUS N/A N BUA ANA 35-37 

276 Pediococcus pentosaceus AN GP-COCCUS N/A N BUA ANA 35-37 

277 Pediococcus urinaeequi AN GP-COCCUS N/A N BUA ANA 35-37 

278 Peptostreptococcus anaerobius AN GP-COCCUS N/A N BUA ANA 35-37 

279 Peptostreptococcus asaccharolyticus AN GP-COCCUS N/A N BUA ANA 35-37 

280 Peptostreptococcus barnesae AN GP-COCCUS N/A N BUA ANA 35-37 

281 Peptostreptococcus hydrogenalis AN GP-COCCUS N/A N BUA ANA 35-37 

282 Peptostreptococcus indolicus AN GP-COCCUS N/A N BUA ANA 35-37 

283 Peptostreptococcus lacrimalis AN GP-COCCUS N/A N BUA ANA 35-37 

284 Peptostreptococcus lactolyticus AN GP-COCCUS N/A N BUA ANA 35-37 

285 Peptostreptococcus prevotii AN GP-COCCUS N/A N BUA ANA 35-37 

286 Peptostreptococcus tetradius AN GP-COCCUS N/A N BUA ANA 35-37 

287 Peptostreptococcus vaginalis AN GP-COCCUS N/A N BUA ANA 35-37 

288 Porphyromonas asaccharolytica AN GN-ROD N/A N BUA ANA 35-37 

289 Porphyromonas circumdentaria/endodontalis AN GN-ROD N/A N BUA ANA 35-37 

290 Porphyromonas gingivalis AN GN-ROD N/A N BUA ANA 35-37 

291 Porphyromonas levii AN GN-ROD N/A N BUA ANA 35-37 

292 Porphyromonas macacae AN GN-ROD N/A N BUA ANA 35-37 

293 Prevotella bivia AN GN-ROD N/A N BUA ANA 35-37 

294 Prevotella buccae AN GN-ROD N/A N BUA ANA 35-37 

295 Prevotella buccalis AN GN-ROD N/A N BUA ANA 35-37 

296 Prevotella corporis AN GN-ROD N/A N BUA ANA 35-37 

297 Prevotella denticola AN GN-ROD N/A N BUA ANA 35-37 

298 Prevotella disiens (GNR Kan-R setup) AN GN-ROD N/A N BUA ANA 35-37 

299 Prevotella disiens (Standard AN setup) AN GN-ROD N/A N BUA ANA 35-37 

300 Prevotella heparinolytica AN GN-ROD N/A N BUA ANA 35-37 

301 Prevotella intermedia AN GN-ROD N/A N BUA ANA 35-37 

302 Prevotella loescheii AN GN-ROD N/A N BUA ANA 35-37 

303 Prevotella nigrescens AN GN-ROD N/A N BUA ANA 35-37 

304 Prevotella oralis AN GN-ROD N/A N BUA ANA 35-37 

305 Prevotella oris (GNR Kan-R setup) AN GN-ROD N/A N BUA ANA 35-37 

306 Prevotella oulora AN GN-ROD N/A N BUA ANA 35-37 

307 Prevotella species AN GN-ROD N/A N BUA ANA 35-37 

308 Propionibacterium acidipropionici* AN GP-ROD N/A N BUA ANA 35-37 

309 Propionibacterium acnes* AN GP-ROD N/A N BUA ANA 35-37 

310 Propionibacterium avidum* AN GP-ROD N/A N BUA ANA 35-37 

311 Propionibacterium freudenreichii ss freudenreichii* AN GP-ROD N/A N BUA ANA 35-37 

312 Propionibacterium granulosum* AN GP-ROD N/A N BUA ANA 35-37 

313 Propionibacterium jensenii* AN GP-ROD N/A N BUA ANA 35-37 

314 Propionibacterium lymphophilum* AN GP-ROD N/A N BUA ANA 35-37 

315 Propionibacterium propionicus* AN GP-ROD N/A N BUA ANA 35-37 

316 Propionibacterium thoenii* AN GP-ROD N/A N BUA ANA 35-37 

317 Pseudoramibacter alactolyticus AN GP-ROD N/A N BUA ANA 35-37 

318 Rikenella microfusus AN GN-ROD N/A N BUA ANA 35-37 

319 Ruminococcus gnavus AN GP-COCCUS N/A N BUA ANA 35-37 

320 Ruminococcus hansenii AN GP-COCCUS N/A N BUA ANA 35-37 

321 Ruminococcus lactaris AN GP-COCCUS N/A N BUA ANA 35-37 

322 Ruminococcus productus AN GP-COCCUS N/A N BUA ANA 35-37 

323 Ruminococcus torques AN GP-COCCUS N/A N BUA ANA 35-37 

324 Sarcina maxima AN GP-COCCUS N/A N BUA ANA 35-37 

325 Sarcina ventriculi AN GP-COCCUS N/A N BUA ANA 35-37 

326 Sebaldella termitidis AN GN-ROD N/A N BUA ANA 35-37 

327 Selenomonas flueggei AN GN-ROD N/A N BUA ANA 35-37 

328 Selenomonas infelix AN GN-ROD N/A N BUA ANA 35-37 

329 Selenomonas noxia AN GN-ROD N/A N BUA ANA 35-37 

330 Selenomonas sputigena AN GN-ROD N/A N BUA ANA 35-37 

331 Slackia heliotrinireducens AN GP-COCCUS N/A N BUA ANA 35-37 

332 Sporolactobacillus inulinus AN GP-ROD N/A N BUA ANA 35-37 

333 Sporomusa acidovorans AN GN-ROD N/A N BUA ANA 35-37 

334 Sporomusa ovata AN GN-ROD N/A N BUA ANA 35-37 

335 Sporomusa sphaeroides AN GN-ROD N/A N BUA ANA 35-37 

336 Staphylococcus aureus ss anaerobius AN GP-COCCUS N/A N BUA ANA 35-37 

337 Staphylococcus saccharolyticus AN GP-COCCUS N/A N BUA ANA 35-37 

338 Streptococcus alactolyticus AN GP-COCCUS N/A N BUA ANA 35-37 

339 Streptococcus anginosus AN GP-COCCUS N/A N BUA ANA 35-37 

340 Streptococcus bovis AN GP-COCCUS N/A N BUA ANA 35-37 

341 Streptococcus macacae AN GP-COCCUS N/A N BUA ANA 35-37 

342 Streptococcus mutans AN GP-COCCUS N/A N BUA ANA 35-37 




343 Streptococcus pleomorphus AN GP-COCCUS N/A N BUA ANA 35-37 

344 Streptococcus suis AN GP-COCCUS N/A N BUA ANA 35-37 

345 Streptococcus thermophilus AN GP-COCCUS N/A N BUA ANA 35-37 

346 Succinivibrio dextrinosolvens AN GN-ROD N/A N BUA ANA 35-37 

347 Sutterella wadsworthensis AN GN-ROD N/A N BUA ANA 35-37 

348 Tetragenococcus halophilus AN GP-COCCUS N/A N BUA ANA 30 

349 Tissierella praeacuta AN GN-ROD N/A N BUA ANA 35-37 

350 Veillonella atypica/caviae/dispar/parvula AN GN-COCCUS N/A N BUA ANA 35-37 

351 Veillonella criceti AN GN-COCCUS N/A N BUA ANA 35-37 

352 Veillonella ratti AN GN-COCCUS N/A N BUA ANA 35-37 

353 Weissella confusa AN GP-COCCUS N/A N BUA ANA 35-37 

354 Weissella halotolerans/hellenica AN GP-ROD N/A N BUA ANA 35-37 

355 Weissella kandleri AN GP-ROD N/A N BUA ANA 35-37 

356 Weissella minor AN GP-ROD N/A N BUA ANA 35-37 

357 Weissella paramesenteroides AN GP-COCCUS N/A N BUA ANA 35-37 

358 Weissella viridescens AN GP-ROD N/A N BUA ANA 35-37 

359 Wolinella succinogenes AN GN-ROD N/A N BUA ANA 35-37 

360 Zymomonas mobilis ss mobilis* AN GN-ROD N/A N BUA ANA 30 

361 Zymophilus paucivorans/raffinosivorans AN GN-ROD N/A N BUA ANA 30 



Yeasts 



1 Arthroascus javanensis N/A N/A N BUY Air 26 

2 Bulleromyces albus N/A N/A N BUY Air 26 

3 Candida aaseri A N/A N/A N BUY Air 26 

4 Candida aaseri B N/A N/A N BUY Air 26 

5 Candida albicans N/A N/A N BUY Air 26 

6 Candida apicola N/A N/A N BUY Air 26 

7 Candida azyma N/A N/A N BUY Air 26 

8 Candida blankii N/A N/A N BUY Air 26 

9 Candida boidinii N/A N/A N BUY Air 26 

10 Candida bombi N/A N/A N BUY Air 26 

11 Candida cantarelli N/A N/A N BUY Air 26 

12 Candida cariosilignicola N/A N/A N BUY Air 26 

13 Candida castellii N/A N/A N BUY Air 26 

14 Candida catenulata N/A N/A N BUY Air 26 

15 Candida diddensiae N/A N/A N BUY Air 26 

16 Candida diversa N/A N/A N BUY Air 26 

17 Candida edax N/A N/A N BUY Air 26 

18 Candida entomophila N/A N/A N BUY Air 26 

19 Candida ergastensis N/A N/A N BUY Air 26 

20 Candida etchellsii N/A N/A N BUY Air 26 

21 Candida famata N/A N/A N BUY Air 26 

22 Candida fluviatilis N/A N/A N BUY Air 26 

23 Candida freyschussii N/A N/A N BUY Air 26 

24 Candida friedrichii N/A N/A N BUY Air 26 

25 Candida fructus N/A N/A N BUY Air 26 

26 Candida fusiformata N/A N/A N BUY Air 26 

27 Candida galacta N/A N/A N BUY Air 26 

28 Candida geochares N/A N/A N BUY Air 26 

29 Candida glabrata N/A N/A N BUY Air 26 

30 Candida glaebosa N/A N/A N BUY Air 26 

31 Candida gropengiesseri N/A N/A N BUY Air 26 

32 Candida haemulonii N/A N/A N BUY Air 26 

33 Candida humilis N/A N/A N BUY Air 26 

34 Candida incommunis N/A N/A N BUY Air 26 

35 Candida insectalens N/A N/A N BUY Air 26 

36 Candida insectamans N/A N/A N BUY Air 26 

37 Candida insectorum N/A N/A N BUY Air 26 

38 Candida intermedia N/A N/A N BUY Air 26 

39 Candida ishiwadae N/A N/A N BUY Air 26 

40 Candida magnoliae N/A N/A N BUY Air 26 

41 Candida maltosa N/A N/A N BUY Air 26 

42 Candida maris N/A N/A N BUY Air 26 

43 Candida maritima N/A N/A N BUY Air 26 

44 Candida melibiosica N/A N/A N BUY Air 26 

45 Candida mogii N/A N/A N BUY Air 26 

46 Candida montana N/A N/A N BUY Air 26 

47 Candida multisgemmis N/A N/A N BUY Air 26 

48 Candida musae N/A N/A N BUY Air 26 

49 Candida nemodendra N/A N/A N BUY Air 26 

50 Candida nitratophila N/A N/A N BUY Air 26 

51 Candida norvegica N/A N/A N BUY Air 26 

52 Candida oleophila N/A N/A N BUY Air 26 

53 Candida parapsilosis A N/A N/A N BUY Air 26 

54 Candida parapsilosis B N/A N/A N BUY Air 26 

55 Candida pararugosa N/A N/A N BUY Air 26 

56 Candida peltata N/A N/A N BUY Air 26 

57 Candida pinus N/A N/A N BUY Air 26 

58 Candida rugosa A N/A N/A N BUY Air 26 

59 Candida rugosa B N/A N/A N BUY Air 26 

60 Candida sake N/A N/A N BUY Air 26 

61 Candida salmanticensis N/A N/A N BUY Air 26 

62 Candida santamariae N/A N/A N BUY Air 26 

63 Candida savonica N/A N/A N BUY Air 26 

64 Candida shehatae N/A N/A N BUY Air 26 

65 Candida shehatae var shehatae N/A N/A N BUY Air 26 




66 Candida silvae N/A N/A N BUY Air 26 

67 Candida silvatica N/A N/A N BUY Air 26 

68 Candida solani N/A N/A N BUY Air 26 

69 Candida sonorensis N/A N/A N BUY Air 26 

70 Candida sorbophila N/A N/A N BUY Air 26 

71 Candida sorboxylosa N/A N/A N BUY Air 26 

72 Candida spandovensis N/A N/A N BUY Air 26 

73 Candida succiphila N/A N/A N BUY Air 26 

74 Candida tropicalis A N/A N/A N BUY Air 26 

75 Candida tropicalis B N/A N/A N BUY Air 26 

76 Candida vanderwaltii N/A N/A N BUY Air 26 

77 Candida vartiovaarai N/A N/A N BUY Air 26 

78 Candida versatilis N/A N/A N BUY Air 26 

79 Candida viswanathii N/A N/A N BUY Air 26 

80 Candida wickerhamii N/A N/A N BUY Air 26 

81 Candida zeylanoides N/A N/A N BUY Air 26 

82 Citeromyces matritensis N/A N/A N BUY Air 26 

83 Clavispora lusitaniae N/A N/A N BUY Air 26 

84 Cryptococcus albidus N/A N/A N BUY Air 26 

85 Cryptococcus albidus var aerius N/A N/A N BUY Air 26 

86 Cryptococcus albidus var albidus N/A N/A N BUY Air 26 

87 Cryptococcus albidus var diffluens N/A N/A N BUY Air 26 

88 Cryptococcus amylolentus N/A N/A N BUY Air 26 

89 Cryptococcus curvatus A N/A N/A N BUY Air 26 

90 Cryptococcus curvatus B N/A N/A N BUY Air 26 

91 Cryptococcus dimennae N/A N/A N BUY Air 26 

92 Cryptococcus gastricus N/A N/A N BUY Air 26 

93 Cryptococcus kuetzingii N/A N/A N BUY Air 26 

94 Cryptococcus laurentii N/A N/A N BUY Air 26 

95 Cryptococcus luteolus N/A N/A N BUY Air 26 

96 Cryptococcus macerans N/A N/A N BUY Air 26 

97 Cryptococcus magnus N/A N/A N BUY Air 26 

98 Cryptococcus magnus var aerius N/A N/A N BUY Air 26 

99 Cryptococcus marinus N/A N/A N BUY Air 26 

100 Cryptococcus skinneri N/A N/A N BUY Air 26 

101 Cryptococcus terreus A N/A N/A N BUY Air 26 

102 Cryptococcus terreus B N/A N/A N BUY Air 26 

103 Cryptococcus tsukubaensis N/A N/A N BUY Air 26 

104 Debaryomyces castellii N/A N/A N BUY Air 26 

105 Debaryomyces hansenii A N/A N/A N BUY Air 26 

106 Debaryomyces hansenii B N/A N/A N BUY Air 26 

107 Debaryomyces hansenii C N/A N/A N BUY Air 26 

108 Debaryomyces hansenii var fabryi N/A N/A N BUY Air 26 

109 Debaryomyces maramus N/A N/A N BUY Air 26 

110 Debaryomyces polymorphus N/A N/A N BUY Air 26 

111 Debaryomyces vanrijiae N/A N/A N BUY Air 26 

112 Dekkera anomala N/A N/A N BUY Air 26 

113 Dekkera bruxellensis A N/A N/A N BUY Air 26 

114 Dekkera bruxellensis B N/A N/A N BUY Air 26 

115 Dekkera custersiana N/A N/A N BUY Air 26 

116 Dekkera naardenensis N/A N/A N BUY Air 26 

117 Dipodascus capitatus N/A N/A N BUY Air 26 

118 Dipodascus ovetensis N/A N/A N BUY Air 26 

119 Endomyces fibuliger N/A N/A N BUY Air 26 

120 Endomycopsella vivi N/A N/A N BUY Air 26 

121 Eremothecium ashbyi N/A N/A N BUY Air 26 

122 Fellomyces fuzhouensis N/A N/A N BUY Air 26 

123 Filobasidiella neoformans bacillisporus N/A N/A N BUY Air 26 

124 Filobasidiella neoformans neoformans A N/A N/A N BUY Air 26 

125 Filobasidiella neoformans neoformans B N/A N/A N BUY Air 26 

126 Filobasidium uniguttulatum N/A N/A N BUY Air 26 

127 Galactomyces geotrichum N/A N/A N BUY Air 26 

128 Geotrichum terrestre N/A N/A N BUY Air 26 

129 Guilliermondella selenospora N/A N/A N BUY Air 26 

130 Hanseniaspora guilliermondii/uvarum/valb N/A N/A N BUY Air 26 

131 Hanseniaspora occidentalis N/A N/A N BUY Air 26 

132 Hanseniaspora osmophila/vineae N/A N/A N BUY Air 26 

133 Hyphopichia burtonii A N/A N/A N BUY Air 26 

134 Hyphopichia burtonii B N/A N/A N BUY Air 26 




135 Hyphopichia burtonii C N/A N/A N BUY Air 26 

136 Issatchenkia orientalis N/A N/A N BUY Air 26 

137 Issatchenkia scutulata N/A N/A N BUY Air 26 

138 Issatchenkia scutulata var exigua N/A N/A N BUY Air 26 

139 Issatchenkia scutulata var scutula N/A N/A N BUY Air 26 

140 Kluyveromyces delphensis N/A N/A N BUY Air 26 

141 Kluyveromyces lactis N/A N/A N BUY Air 26 

142 Kluyveromyces lodderae N/A N/A N BUY Air 26 

143 Kluyveromyces marxianus N/A N/A N BUY Air 26 

144 Kluyveromyces thermotolerans N/A N/A N BUY Air 26 

145 Kluyveromyces wickerhamii N/A N/A N BUY Air 26 

146 Kurtzmanomyces nectairei N/A N/A N BUY Air 26 

147 Lodderomyces elongisporus N/A N/A N BUY Air 26 

148 Metschnikowia pulcherrima N/A N/A N BUY Air 26 

149 Metschnikowia reukaufii N/A N/A N BUY Air 26 

150 Metschnikowia zobellii N/A N/A N BUY Air 26 

151 Nadsonia fulvescens N/A N/A N BUY Air 26 

152 Pachysolen tannophilus N/A N/A N BUY Air 26 

153 Phaffia rhodozyma N/A N/A N BUY Air 26 

154 Pichia alni N/A N/A N BUY Air 26 

155 Pichia amenthionina var amethonina N/A N/A N BUY Air 26 

156 Pichia amenthionina var pachy N/A N/A N BUY Air 26 

157 Pichia amylophila/mississippiensis N/A N/A N BUY Air 26 

158 Pichia angusta N/A N/A N BUY Air 26 

159 Pichia anomala N/A N/A N BUY Air 26 

160 Pichia bispora N/A N/A N BUY Air 26 

161 Pichia canadensis N/A N/A N BUY Air 26 

162 Pichia carsonii N/A N/A N BUY Air 26 

163 Pichia etchellsii N/A N/A N BUY Air 26 

164 Pichia fabianii N/A N/A N BUY Air 26 

165 Pichia farinosa/muscicola N/A N/A N BUY Air 26 

166 Pichia fermentans N/A N/A N BUY Air 26 

167 Pichia fluxuum N/A N/A N BUY Air 26 

168 Pichia glucozyma/methanolica N/A N/A N BUY Air 26 

169 Pichia guilliermondii A N/A N/A N BUY Air 26 

170 Pichia guilliermondii B N/A N/A N BUY Air 26 

171 Pichia haplophila N/A N/A N BUY Air 26 

172 Pichia holstii N/A N/A N BUY Air 26 

173 Pichia jadinii N/A N/A N BUY Air 26 

174 Pichia kluyveri N/A N/A N BUY Air 26 

175 Pichia media N/A N/A N BUY Air 26 

176 Pichia membranaefaciens N/A N/A N BUY Air 26 

177 Pichia mexicana N/A N/A N BUY Air 26 

178 Pichia minuta N/A N/A N BUY Air 26 

179 Pichia muscicola N/A N/A N BUY Air 26 

180 Pichia norvegensis N/A N/A N BUY Air 26 

181 Pichia ohmeri A N/A N/A N BUY Air 26 

182 Pichia ohmeri B N/A N/A N BUY Air 26 

183 Pichia onychis N/A N/A N BUY Air 26 

184 Pichia opuntiae N/A N/A N BUY Air 26 

185 Pichia pastoris N/A N/A N BUY Air 26 

186 Pichia petersonii N/A N/A N BUY Air 26 

187 Pichia pijperi N/A N/A N BUY Air 26 

188 Pichia pini N/A N/A N BUY Air 26 

189 Pichia rabaulensis N/A N/A N BUY Air 26 

190 Pichia rhodanensis N/A N/A N BUY Air 26 

191 Pichia silvicola N/A N/A N BUY Air 26 

192 Pichia spartinae N/A N/A N BUY Air 26 

193 Pichia stipitis N/A N/A N BUY Air 26 

194 Pichia subpelliculosa N/A N/A N BUY Air 26 

195 Pichia sydowiorum N/A N/A N BUY Air 26 

196 Pichia thermotolerans N/A N/A N BUY Air 26 

197 Pichia toletana N/A N/A N BUY Air 26 

198 Pichia trehalophila N/A N/A N BUY Air 26 

199 Pichia triangularis N/A N/A N BUY Air 26 

200 Rhodosporidium diobovatum N/A N/A N BUY Air 26 

201 Rhodosporidium sphaerocarpum N/A N/A N BUY Air 26 

202 Rhodosporidium toruloides N/A N/A N BUY Air 26 




203 Rhodotorula acheniorum N/A N/A N BUY Air 26 

204 Rhodotorula acuta N/A N/A N BUY Air 26 

205 Rhodotorula araucariae N/A N/A N BUY Air 26 

206 Rhodotorula aurantiaca A N/A N/A N BUY Air 26 

207 Rhodotorula aurantiaca B N/A N/A N BUY Air 26 

208 Rhodotorula bacarum N/A N/A N BUY Air 26 

209 Rhodotorula glutinis N/A N/A N BUY Air 26 

210 Rhodotorula glutinis var glutinis N/A N/A N BUY Air 26 

211 Rhodotorula graminis N/A N/A N BUY Air 26 

212 Rhodotorula hylophila N/A N/A N BUY Air 26 

213 Rhodotorula minuta N/A N/A N BUY Air 26 

214 Rhodotorula minuta var minuta N/A N/A N BUY Air 26 

215 Rhodotorula mucilaginosa N/A N/A N BUY Air 26 

216 Rhodotorula muscorum N/A N/A N BUY Air 26 

217 Rhodotorula philyla N/A N/A N BUY Air 26 

218 Rhodotorula pustula N/A N/A N BUY Air 26 

219 Saccharomyces bayanus N/A N/A N BUY Air 26 

220 Saccharomyces boulardii N/A N/A N BUY Air 26 

221 Saccharomyces cerevisiae A/Tor.pretorien N/A N/A N BUY Air 26 

222 Saccharomyces cerevisiae B N/A N/A N BUY Air 26 

223 Saccharomyces dairensis N/A N/A N BUY Air 26 

224 Saccharomycodes ludwigii N/A N/A N BUY Air 26 

225 Saccharomycopsis capsularis N/A N/A N BUY Air 26 

226 Saturnospora dispora N/A N/A N BUY Air 26 

227 Schizoblastosporon starkeyi-henricii N/A N/A N BUY Air 26 

228 Schizosaccharomyces japonicus N/A N/A N BUY Air 26 

229 Schizosaccharomyces japonicus var japoni N/A N/A N BUY Air 26 

230 Schizosaccharomyces octosporus N/A N/A N BUY Air 26 

231 Schizosaccharomyces pombe N/A N/A N BUY Air 26 

232 Schizosaccharomyces pombe var malidevora N/A N/A N BUY Air 26 

233 Schwanniomyces occidentalis N/A N/A N BUY Air 26 

234 Sporidiobolus johnsonii A N/A N/A N BUY Air 26 

235 Sporidiobolus johnsonii B N/A N/A N BUY Air 26 

236 Sporidiobolus johnsonii C N/A N/A N BUY Air 26 

237 Sporidiobolus pararoseus A N/A N/A N BUY Air 26 

238 Sporidiobolus pararoseus B N/A N/A N BUY Air 26 

239 Sporobolomyces albo-rubescens N/A N/A N BUY Air 26 

240 Sporopachydermia cereana N/A N/A N BUY Air 26 

241 Sporopachydermia lactativora N/A N/A N BUY Air 26 

242 Stephanoascus ciferrii N/A N/A N BUY Air 26 

243 Sterigmatomyces elviae N/A N/A N BUY Air 26 

244 Sterigmatomyces halophilus N/A N/A N BUY Air 26 

245 Torulaspora delbrueckii N/A N/A N BUY Air 26 

246 Torulaspora globosa N/A N/A N BUY Air 26 

247 Trichosporon beigelii A N/A N/A N BUY Air 26 

248 Trichosporon beigelii B N/A N/A N BUY Air 26 

249 Trichosporon brassicae N/A N/A N BUY Air 26 

250 Trichosporon inkin N/A N/A N BUY Air 26 

251 Trigonopsis triangularis/variabilis N/A N/A N BUY Air 26 

252 Ustilago maydis N/A N/A N BUY Air 26 

253 Wickerhamiella domercqiae N/A N/A N BUY Air 26 

254 Williopsis californica N/A N/A N BUY Air 26 

255 Williopsis saturnus N/A N/A N BUY Air 26 

256 Williopsis saturnus var mrakii N/A N/A N BUY Air 26 

257 Williopsis saturnus var saturnus N/A N/A N BUY Air 26 

258 Wingea robertsiae N/A N/A N BUY Air 26 

259 Yarrowia lipolytica N/A N/A N BUY Air 26 

260 Zygoascus hellenicus N/A N/A N BUY Air 26 

261 Zygosaccharomyces bailii N/A N/A N BUY Air 26 

262 Zygosaccharomyces bisporus N/A N/A N BUY Air 26 

263 Zygosaccharomyces cidri N/A N/A N BUY Air 26 

264 Zygosaccharomyces fermentati N/A N/A N BUY Air 26 

265 Zygosaccharomyces florentinus N/A N/A N BUY Air 26 

266 Zygosaccharomyces microellipsoides/mraki N/A N/A N BUY Air 26 

267 Zygosaccharomyces rouxii N/A N/A N BUY Air 26 



Filamentous Fungi (and select Yeasts) 

Species Name Type Medium Atm Temp Photo 

1 Absidia californica J.Ellis & Hesseltine FF-AIR 2%ME Air 26 Mm 

2 Absidia corymbifera (Cohn) Sacc. & Trotter FF-AIR, FOOD 2%ME Air 26 

3 Absidia clindrospora var clindrospors Hagen FF-AIR 2%ME Air 26 m 

4 Absidia glauca Hagen FF-AIR 2%ME Air 26 Mm 

5 Absidia spinosa var spinosa Lendner FF-AIR 2%ME Air 26 

6 Acremonium atrogrisum (Panasenko) W. Gams FF-AIR 2%ME Air 26 M 

7 Acremonium bacillisporum (Onions & Barron) W.Gams FF-AIR 2%ME Air 26 M 

8 Acremonium berkeleyanum (P. Karsten) W. Gams FF-FOOD 2%ME Air 26 M 

9 Acremonium breve (Sukapure & Thirumalachar) W. Gams BGA FF-AIR 2%ME Air 26 M 

10 Acremonium breve (Sukapure & Thirumalachar) W. Gams BGB FF-AIR 2%ME Air 26 M 

11 Acremonium charticola (Lindau) W. Gams FF-AIR, FOOD 2%ME Air 26 M 

12 Acremonium furcatum (F. & V. Moreau) ex W. Gams FF-AIR 2%ME Air 26 M 

13 Acremonium fusidioides (Nicot) W.Gams FF-AIR 2%ME Air 26 M 

14 Acremonium hyalinulum FF-AIR 2%ME Air 26 

15 Acremonium kiliense Gruetz FF-AIR 2%ME Air 26 M 

16 Acremonium murorum var felinum (Marchal) S. Hughes FF-AIR 2%ME Air 26 M 

17 Acremonium murorum var murorum (Corda) W. Gams FF-AIR 2%ME Air 26 

18 Acremonium polychromum (v. Beyma) W. Gams FF-AIR 2%ME Air 26 

19 Acremonium potronii Vuill. FF-AIR 2%ME Air 26 M 

20 Acremonium strictum W. Gams BGA FF-AIR, FOOD 2%ME Air 26 M 

21 Acremonium strictum W. Gams BGB FF-AIR, FOOD 2%ME Air 26 M 

22 Acrodontium crateriforme (van Beyma) de Hoog FF-AIR 2%ME Air 26 M 

23 Acrodontium griseum (Fassatiova’) de Hoog FF-AIR 2%ME Air 26 

24 Acrodontium salmoneum de Hoog BGA FF-AIR 2%ME Air 26 M 

25 Acrodontium salmoneum de Hoog BGB FF-AIR 2%ME Air 26 M 

26 Acrodontium simplex (Mangenot) de Hoog FF-AIR 2%ME Air 26 M 

27 Actinomucor elegans (Eidam) C.R. Benjamin & Hesseltine BGA FF-AIR 2%ME Air 26 Mm 

28 Actinomucor elegans (Eidam) C.R. Benjamin & Hesseltine BGB FF-AIR 2%ME Air 26 Mm 

29 Alternaria alternata (Fr.) Keissl FF-AIR, FOOD 2%ME Air 26 Mm 

30 Amorphotheca resinae D. Parbery FF-AIR 2%ME Air 26 M 

31 Aphanocladium album (Preuss) W. Gams FF-AIR 2%ME Air 26 M 

32 Apiospora montagnei Sacc. FF-AIR 2%ME Air 26 M 

33 Arthrinium phacospermum (Corda) M.B. Ellis FF-AIR, FOOD 2%ME Air 26 M 

34 Aspergillus aculeatus Izuka FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

35 Aspergillus aeneus Sappa FF-ASP 2%ME Air 26 M 

36 Aspergillus asperescens Stolk FF-AIR, ASP 2%ME Air 26 Mm 

37 Aspergillus aureolatus Muntanola-Cvctkovic & Bata BGA FF-AIR, ASP 2%ME Air 26 Mm 

38 Aspergillus aureolatus Muntanola-Cvctkovic & Bata BGB FF-AIR, ASP 2%ME Air 26 Mm 

39 Aspergillus auricomus (Guegen) Saito FF-ASP 2%ME Air 26 Mm 

40 Aspergillus avenaceus G. Smith FF-ASP 2%ME Air 26 M 

41 Aspergillus awamori Nakazawa FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

42 Aspergillus brevipes G.Smith FF-ASP 2%ME Air 26 M 

43 Aspergillus caesiellus Saito FF-AIR, ASP 2%ME Air 26 Mm 

44 Aspergillus caespitosus Raper & Thom FF-ASP 2%ME Air 26 M 

45 Aspergillus candidus Link BGA FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

46 Aspergillus candidus Link BGB FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

47 Aspergillus carbonarius (Bainier) Thom FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

48 Aspergillus carneus (V. Tiegham) Blockwitz FF-AIR, ASP 2%ME Air 26 Mm 

49 Aspergillus cervinus Massee FF-ASP 2%ME Air 26 M 

50 Aspergillus clavatus Desm. FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

51 Aspergillus egyptiacus Moubasher & Moustafa FF-ASP 2%ME Air 26 M 

52 Aspergillus elegans Gasperini FF-AIR, ASP 2%ME Air 26 Mm 

53 Aspergillus flavofurcatus Batista & Maia FF-ASP 2%ME Air 26 Mm 

54 Aspergillus flavus var flavus Link FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

55 Aspergillus foetidus Thom & Raper FF-ASP 2%ME Air 26 M 

56 Aspergillus fresenii Subramanian FF-AIR, ASP 2%ME Air 26 Mm 

57 Aspergillus fumigatus Fresen. FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

58 Aspergillus furniculosus G. Smith FF-ASP 2%ME Air 26 M 

59 Aspergillus giganteus Wehmer FF-AIR, ASP 2%ME Air 26 Mm 

60 Aspergillus japonicus var japonicus K. Saito FF-AIR, ASP 2%ME Air 26 Mm 

61 Aspergillus kanagawaensis Nehira BGA FF-ASP 2%ME Air 26 M 

62 Aspergillus kanagawaensis Nehira BGB FF-ASP 2%ME Air 26 M 

63 Aspergillus lanosus Kamal & Bhargava FF-ASP 2%ME Air 26 M 

64 Aspergillus malignus Lindt FF-ASP 2%ME Air 26 M 

M Indicates that a Macroscopic photo is present in Biolog’s photo library. 

m Indicates that a Microscopic photo is present in Biolog’s photo library. 

MicroStation System/MicroLog User Guide Apr 07 Section 13� Page 29



65 Aspergillus niger v. Tiegham BGA FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

66 Aspergillus niger v. Tiegham BGB FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

67 Aspergillus nutans Mclennan & Ducker FF-ASP 2%ME Air 26 Mm 

68 Aspergillus ochraceus K. Wilhelm BGA FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

69 Aspergillus ochraceus K. Wilhelm BGB FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

70 Aspergillus oryzae var oryzae (Ahlburg) Cohn FF-AIR, FOOD 2%ME Air 26 Mm 

71 Aspergillus ostianus Wehmer FF-AIR, ASP 2%ME Air 26 Mm 

72 Aspergillus pallidus Kamyschko FF-ASP 2%ME Air 26 Mm 

73 Aspergillus parasiticus Speare BGA FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

74 Aspergillus parasiticus Speare BGB FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

75 Aspergillus parvulus G. Sm. BGA FF-ASP 2%ME Air 26 M 

76 Aspergillus parvulus G. Sm. BGB FF-ASP 2%ME Air 26 M 

77 Aspergillus penicilliformis Kamyschko FF-ASP 2%ME Air 26 M 

78 Aspergillus petrakii Voros FF-ASP 2%ME Air 26 M 

79 Aspergillus phoenicis (Corda) Thom FF-ASP 2%ME Air 26 M 

80 Aspergillus proliferans G. Smith FF-ASP 2%ME Air 26 M 

81 Aspergillus pulverulentus (McAlp.) Thom FF-ASP 2%ME Air 26 Mm 

82 Aspergillus puniceus Kwon & Fennell FF-AIR, ASP 2%ME Air 26 Mm 

83 Aspergillus raperi Stolk & J.A. Meyer FF-AIR, ASP 2%ME Air 26 M 

84 Aspergillus restrictus G. Smith FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

85 Aspergillus sclerotiorum Huber FF-AIR, ASP 2%ME Air 26 Mm 

86 Aspergillus sepultus Tuthill & Christensen FF-ASP 2%ME Air 26 M 

87 Aspergillus sparsus Raper & Thom FF-ASP 2%ME Air 26 M 

88 Aspergillus speluneus Raper & Fennell FF-ASP 2%ME Air 26 Mm 

89 Aspergillus sydowii (Brainier & Sartory) Thom & Church FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

90 Aspergillus tamarii Kita FF-FOOD, ASP 2%ME Air 26 M 

91 Aspergillus terreus var africanus Fennell & Rapier FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

92 Aspergillus terreus var terreus Thom FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

93 Aspergillus terricola var americanus Marchal FF-AIR, FOOD, ASP 2%ME Air 26 m 

94 Aspergillus terricola var terricola Marchal FF-AIR, FOOD, ASP 2%ME Air 26 m 

95 Aspergillus ustus (Bainier) Thom & Church FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

96 Aspergilus versicolor (Vuill.) Tirab. FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

97 Aspergillus violaceofuscus Gasperini FF-ASP 2%ME Air 26 m 

98 Aspergillus wentii Wermer FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

99 Aspergillus zonatus Zonatus FF-ASP 2%ME Air 26 M 

100 Aureobasidium pullulans var melanigenum (de Barry) G. Arnaud FF-AIR, FOOD 2%ME Air 26 M 

101 Aureobasidium pullulans var pullulans (de Barry) G. Arnaud FF-AIR, FOOD 2%ME Air 26 Mm 

102 Basipetospora halophila (J.F.H. Beyma) Pitt & A.D. Hocking FF-FOOD 2%ME Air 26 M 

103 Beauveria bassiana FF-AIR 2%ME Air 26 M 

104 Blastobotrys elegans de Hoog et al. BGA FF-AIR 2%ME Air 26 M 

105 Blastobotrys elegans de Hoog et al. BGB FF-AIR 2%ME Air 26 M 

106 Botryosporium longibrachiatum (Oud.) Maire FF-AIR 2%ME Air 26 M 

107 Botrytis aclada Fresen. FF-AIR, FOOD 2%ME Air 26 Mm 

108 Botrytis cinerea Pers. BGA FF-AIR, FOOD 2%ME Air 26 Mm 

109 Botrytis cinerea Pers. BGB FF-AIR, FOOD 2%ME Air 26 Mm 

110 Bulleromyces albus FF-AIR, YST 2%ME Air 26 Mm 

111 Byssochlamys fulva Olliver & G. Smith BGA FF-AIR, FOOD 2%ME Air 26 M 

112 Byssochlamys fulva Olliver & G. Smith BGB FF-AIR, FOOD 2%ME Air 26 M 

113 Byssochlamys nivea Westling FF-AIR, FOOD 2%ME Air 26 M 

114 Candida albicans (Robin) Berkhout FF-YST 2%ME Air 26 

115 Candida guilliermondii (Castellani) Langeron & Guerra Kodamaea ohmeri) FF-FOOD, YST 2%ME Air 26 

(teleo. Kodamaea ohmeri) 

116 Candida insectorum D.B. Scott et al. FF-YST 2%ME Air 26 

117 Candida intermedia(AS) (Ciferri & Ashford) Langeron & Guerra FF-FOOD, YST 2%ME Air 26 Mm 

118 Candida montana S. Goto & Oguri FF-YST 2%ME Air 26 

119 Candida parapsilosis (Ashford) Langeron & Talice FF-FOOD, YST 2%ME Air 26 M 

120 Candida rugosa (H.W. Anderson) Didddens & Lodder FF-YST 2%ME Air 26 

121 Candida sake (Saito & Oda) v. Uden & Buckley FF-FOOD, YST 2%ME Air 26 Mm 

122 Candida sorbophila (Nakase) S.A. Meyer & Yarrow FF-YST 2%ME Air 26 

123 Candida zeylanoides (Castellani) Langeron & Guerra FF-FOOD, YST 2%ME Air 26 Mm 

124 Cephaliophora tropica Thaxler FF-AIR 2%ME Air 26 Mm 

125 Cerinosterus cyanesens (de Hoog & de Vries) R.T. Moore FF-AIR 2%ME Air 26 

126 Chaetomium funicola Cooke FF-FOOD 2%ME Air 26 

127 Chaetomium globosum Kunze: Fries FF-AIR, FOOD 2%ME Air 26 M 

128 Chaetosartorya stromatoides Wiley & Simmons FF-ASP 2%ME Air 26 M 

129 Chrysosporium indicum (Randhawa & Sandhu) Garg FF-AIR 2%ME Air 26 M 

(anam. Aspergillus stromatoides) 



Section 13 � Page30 MicroStation System/MicroLog User Guide Apr 07



130 Chrysosporium inops J.W. Carmich. FF-FOOD 2%ME Air 26 M 

131 Chrysosporium keratinophilum D.Frey ex Charmichael FF-FOOD 2%ME Air 26 

132 Chrysosporium merdarium (Link: Fries) Carmichael. FF-AIR 2%ME Air 26 

133 Chrysosporium pannicola (Corda) van Oorschot & Stalpers FF-AIR 2%ME Air 26 

134 Cladosporium cladosporiodes (Fresen.) G.A. de Vries FF-AIR, FOOD 2%ME Air 26 Mm 

135 Cladosporium herbarum (Pers.) Link BGA FF-AIR, FOOD 2%ME Air 26 Mm 

136 Cladosporium herbarum (Pers.) Link BGB FF-AIR, FOOD 2%ME Air 26 Mm 

137 Cladosporium macrocarpum Preuss FF-AIR, FOOD 2%ME Air 26 M 

138 Cladosporium sphaerospermum Penz. BGA FF-AIR, FOOD 2%ME Air 26 Mm 

139 Cladosporium sphaerospermum Penz. BGB FF-AIR, FOOD 2%ME Air 26 Mm 

140 Cladosporium tenuissium Cooke FF-AIR 2%ME Air 26 M 

141 Clonostachys rosea (Link: Fr.) Schroers, Samuels, Seifert & W. Gams FF-AIR 2%ME Air 26 Mm 

142 Colletotrichum acutatum Simmonds FF-COL 2%ME Air 26 m 

143 Colletotrichum capsici (H. Sydow) E. Butler & Bisby FF-COL 2%ME Air 26 

144 Colletotrichum coccodes (Wallroth) Hughes FF-COL 2%ME Air 26 

145 Colletotrichum coffeanum Noack sensu Hindorf FF-COL 2%ME Air 26 

146 Colletotrichum crassipes (Spegazzini) von Arx FF-COL 2%ME Air 26 

147 Colletotrichum dematium (Persoon: Fries) Grove FF-COL 2%ME Air 26 

148 Colletotrichum destructivum O’Gara FF-COL 2%ME Air 26 

149 Colletotrichum gloeosporiodes (Penzig) Penzig & Saccardo FF-FOOD, COL 2%ME Air 26 M 

150 Colletotrichum lindenuthianum (Saccardo & Magnus) Briosi FF-COL 2%ME Air 26 

151 Colletotrichum trichellum (Fries) Duke FF-COL 2%ME Air 26 

152 Colletotrichum trifolii Bain & Essary FF-COL 2%ME Air 26 

153 Colletotrichum truncatum (Schweinitz) Andrus & Moore BGA FF-COL 2%ME Air 26 

154 Colletotrichum truncatum (Schweinitz) Andrus & Moore BGB FF-COL 2%ME Air 26 

155 Cryptococcus albidus var albidus (Saito) Skinner FF-FOOD, YST 2%ME Air 26 Mm 

156 Cryptococcus laurentii var laurentii (Kufferath) Skinner FF-FOOD, YST 2%ME Air 26 Mm 

157 Cryptococcus terreus di Menna FF-YST 2%ME Air 26 

158 Cunninghamella elegans Lendner FF-AIR, FOOD 2%ME Air 26 

159 Curvularia lunata var lunata (Wakker) Boedijn BGA FF-AIR, FOOD 2%ME Air 26 Mm 

160 Curvularia lunata var lunata (Wakker) Boedijn BGB FF-AIR, FOOD 2%ME Air 26 Mm 

161 Debaryomyces castellii Capriotti FF-YST 2%ME Air 26 

162 Debaryomyces hansenii var hansenii (Zopl) Lodder & Kreger-v.Rij BGA FF-FOOD, YST 2%ME Air 26 Mm 

(anam. CANADA FAMATA) 

163 Debaryomyces hansenii var hansenii (Zopl) Lodder & Kreger-v.Rij BGB FF-FOOD, YST 2%ME Air 26 Mm 


164 Debaryomyces hansenii var hansenii (Zopl) Lodder & Kreger-v.Rij BGC FF-FOOD, YST 2%ME Air 26 Mm 


165 Debaryomyces hansenii var hansenii (Zopl) Lodder & Kreger-v.Rij BGD FF-FOOD, YST 2%ME Air 26 Mm 


166 Debaryomyces occidentalis var occidentalis (Klocker) Kurtzman & Robnett FF-YST 2%ME Air 26 

167 Debaryomyces polymorphus var polymorphus (Klocker) C.W. Price & Phaff FF-YST 2%ME Air 26 

168 Dekkera bruxellensis van der Walt (anam. Brettanomyces bruxellensis) FF-FOOD, YST 2%ME Air 26 Mm 

169 Doratomyces microsporus (Sacc.) Morton & G. Smith FF-AIR 2%ME Air 26 M 

170 Doratomyces stemonitis (Pers.:Fr) Morton & G. Smith FF-AIR 2%ME Air 26 M 

171 Emericella fruticulosa (Raper & Fennel) Malloch & Cain FF-ASP 2%ME Air 26 M 

(anam.Aspergillus truticans) 

172 Emericella nidulans var nidulans (Eidam) Vuillemin FF-AIR, FOOD, ASP 2%ME Air 26 M 

(anam. Aspergillus nidulellus) 

173 Emericella quadrilineata (Thom & Raper) C.R. Benjamin FF-ASP 2%ME Air 26 M 

(anam. Aspergillus tetrazonus) 

174 Emericella rugulosa (Thom & Raper) C.R. Benjamin FF-ASP 2%ME Air 26 M 

(anam. Aspergillus rugulovalvus) 

175 Emericella striata (J.N. Rai et al.) Malloch & Cain FF-ASP 2%ME Air 26 M 

(anam. Aspergillus striatulus) 

176 Emericella unguis Malloch & Cain BGA (anam. Aspergillus unguis) FF-ASP 2%ME Air 26 M 

177 Emericella unguis Malloch & Cain BGB (anam. Aspergillus unguis) FF-ASP 2%ME Air 26 M 

178 Emericella variecolor var variecolor Berk. & Br. In Berk. FF-ASP 2%ME Air 26 M 

(anam. Aspergillus stellifer) 

179 Emericella violacea (Fennell & Raper) Malloch & Cain FF-ASP 2%ME Air 26 M 

(anam. Aspergillus violaccobrunneus) 

180 Endomyces fibuliger Lindner FF-AIR, FOOD, YST 2%ME Air 26 Mm 

181 Engyodontium album (Limber) de Hoog FF-AIR 2%ME Air 26 M 

182 Epicoccum nigrum (purapurascens) Link FF-AIR 2%ME Air 26 M 

183 Eremascus fertilis Stoppel FF-AIR, FOOD, YST 2%ME Air 26 M 

184 Eupenicillium baarnense (V.Beyma)Stolk&Scott (anam. Penicillium turbatum) FF-PEN 2%ME Air 26 M 

185 Eupenicillium brefeldianum (Dodge)Stolk&Scott (anam. Penicillium dodgei) FF-PEN 2%ME Air 26 M 






186 Eupenicillium cinnamorpurpureum D.B. Scott & Stolk BGA FF-AIR, FOOD, PEN 2%ME Air 26 M 

(anam. Penicillium cinnamopurpureum) 

187 Eupenicillium cinnamorpurpureum D.B. Scott & Stolk BGB FF-AIR, FOOD, PEN 2%ME Air 26 M 

(anam. Penicillium cinnamopurpureum) 

188 Eupenicillium crustaceum Ludwig (anam. Penicillium nilense) FF-AIR, PEN 2%ME Air 26 M 

189 Eupenicillium euglaucum (van Beyma) Stolk & Samson BGA FF-PEN 2%ME Air 26 Mm 

(anam. Penicillium citreonigrum) 

190 Eupenicillium euglaucum (van Beyma) Stolk & Samson BGB FF-PEN 2%ME Air 26 Mm 

(anam. Penicillium citreonigrum) 

191 Eupenicillium javanicum var javanicum (Van Beyma) Stolk & Scott FF-AIR, FOOD, PEN 2%ME Air 26 M 

(P. simplicissium p.p.) 

192 Eupenicillium lapidosum Scott & Stolk (anam. Penicillium lapidosum) FF-PEN 2%ME Air 26 M 

193 Eupenicillium meridianum Scott (anam. Penicillium decumbens) FF-PEN 2%ME Air 26 M 

194 Eupenicillium pinetorum Stolk (anam. Penicillium fuscum) FF-PEN 2%ME Air 26 M 

195 Eurotium amstelodami Mangin (anam. Aspergillus hollandicus) FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

196 Eurotium chevalieri L.Mangin BGA (anam. Aspergillus equitis) FF-AIR, FOOD, ASP 2%ME Air 26 M 

197 Eurotium chevalieri L.Mangin BGB (anam. Aspergillus equitis) FF-AIR, FOOD, ASP 2%ME Air 26 M 

198 Eurotium echinulatum Delacroix (anam. Aspergillus echinulatus) FF-AIR, ASP 2%ME Air 26 M 

199 Eurotium herbariorum (Wiggers:Fr.) Link (anam. Aspergillus glaucus) FF-AIR, FOOD, ASP 2%ME Air 26 M 

200 Eurotium intermedium Blaser (anam. Aspergillus intermedium) FF-ASP 2%ME Air 26 Mm 

201 Eurotium repens FF-AIR, FOOD, ASP 2%ME Air 26 M 

202 Eurotium rubrum Konig et al. (anam. Aspergillus rubrobrunneus) FF-AIR, FOOD, ASP 2%ME Air 26 Mm 

203 Eurotium spiculosum Blaser BGA (anam. Aspergillus spiculosum) FF-ASP 2%ME Air 26 

204 Eurotium spiculosum Blaser BGB (anam. Aspergillus spiculosum) FF-ASP 2%ME Air 26 

205 Fennellia flavipes Wiley & Simmons (anam. Aspergillus flavipes) FF-FOOD, ASP 2%ME Air 26 M 

206 Fennellia nivea (Wiley & Simmons) Samson BGA (anam. Aspergillus niveus) FF-FOOD, ASP 2%ME Air 26 M 

207 Fennellia nivea (Wiley & Simmons) Samson BGB (anam. Aspergillus niveus) FF-FOOD, ASP 2%ME Air 26 M 

208 Fusarium acuminatum Ellis & Everh. BGA (teleo. Gibberella acuminatum) FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

209 Fusarium acuminatum Ellis & Everh. BGB (teleo. Gibberella acuminatum) FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

210 Fusarium annulatum Bugnicourt FF-FUS 2%ME Air 26 M 

211 Fusarium avenaceum (Corda:Fr.) Sacc. BGA (teleo. Gibberella avenacea) FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

212 Fusarium avenaceum (Corda:Fr.) Sacc. BGB (teleo. Gibberella avenacea) FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

213 Fusarium avenaceum (Corda:Fr.) Sacc. BGC (teleo. Gibberella avenacea) FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

214 Fusarium avenaceum s sp aywerte Sangalang & L.W. Burgess FF-FUS 2%ME Air 26 Mm 

215 Fusarium avenaceum s sp nurragai Summerell & L.W. Burgess FF-FUS 2%ME Air 26 Mm 

216 Fusarium brevicatenulatum Nirenberg, O’Donnell, Kroschel & Andrianairo FF-FUS 2%ME Air 26 M 

217 Fusarium camptoceras Wollenw. & Reinking FF-FUS 2%ME Air 26 M 

218 Fusarium chlamydosporum var chlamydosporum Wollenw. & Reinking FF-AIR, FOOD, FUS 2%ME Air 26 M 

219 Fusarium ciliatum Link FF-FUS 2%ME Air 26 M 

220 Fusarium compactum (Wollenw.) Raillo FF-FUS 2%ME Air 26 M 

221 Fusarium crookwellense Burgess, Nelson & Toussoun BGA FF-FUS 2%ME Air 26 M 

222 Fusarium crookwellense Burgess, Nelson & Toussoun BGB FF-FUS 2%ME Air 26 M 

223 Fusarium crookwellense Burgess, Nelson & Toussoun BGC FF-FUS 2%ME Air 26 M 

224 Fusarium culmorum (W.G. Smith) Sacc. BGA FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

225 Fusarium culmorum (W.G. Smith) Sacc. BGB FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

226 Fusarium decemcellulare Brick (teleo. Nectria rigidiuscula) FF-FUS 2%ME Air 26 M 

227 Fusarium equiseti (Corda) Sacc. (teleo. Gibberella intricans) FF-AIR, FOOD, FUS 2%ME Air 26 M 

228 Fusarium eumartii Carpenter (teleo. Nectria haematococca) FF-FUS 2%ME Air 26 M 

229 Fusarium flocciferum Corda (teleo. Gibberella heterochroma) FF-FUS 2%ME Air 26 M 

230 Fusarium fujikuroi Nirenberg (teleo. Gibberella fujikuroi) FF-FUS 2%ME Air 26 M 

231 Fusarium globosum Rheeder, Marasas & Nelson FF-FUS 2%ME Air 26 M 

232 Fusarium graminearum Schwabe BGA (teleo. Gibberella zeae) FF-AIR, FOOD, FUS 2%ME Air 26 M 

233 Fusarium graminearum Schwabe BGB (teleo. Gibberella zeae) FF-AIR, FOOD, FUS 2%ME Air 26 M 

234 Fusarium graminearum Schwabe BGC (teleo. Gibberella zeae) FF-AIR, FOOD, FUS 2%ME Air 26 M 

235 Fusarium graminearum Schwabe BGD (teleo. Gibberella zeae) FF-AIR, FOOD, FUS 2%ME Air 26 M 

236 Fusarium graminearum Schwabe BGE (teleo. Gibberella zeae) FF-AIR, FOOD, FUS 2%ME Air 26 M 

237 Fusarium graminum Corda FF-AIR, FOOD, FUS 2%ME Air 26 M 

238 Fusarium heterosporum Nees BGA (teleo. Gibberella gordonia) FF-FUS 2%ME Air 26 M 

239 Fusarium heterosporum Nees BGB (teleo. Gibberella gordonia) FF-FUS 2%ME Air 26 M 

240 Fusarium heterosporum Nees BGC (teleo. Gibberella gordonia) FF-FUS 2%ME Air 26 M 

241 Fusarium juruanum P. Henn. (teleo. Nectria diplos) FF-FUS 2%ME Air 26 M 

242 Fusarium lactis Pirotta & Riboni FF-FUS 2%ME Air 26 M 

243 Fusarium lateritium var lateritium Nees (teleo. Gibberella baccata) FF-FUS 2%ME Air 26 M 

244 Fusarium longipes Wollenw. & Reinking FF-FOOD, FUS 2%ME Air 26 Mm 

245 Fusarium melanochlorum (Caspary) Sacc. (teleo. Nectria flavoviridis) FF-FUS 2%ME Air 26 M 

246 Fusarium merismoides VAR MERISMOIDES Corda FF-AIR, FUS 2%ME Air 26 M 






247 Fusarium oxysporum Schlcct.:Fr. BGA FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

248 Fusarium oxysporum Schlcct.:Fr. BGB FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

249 Fusarium pallidoroseum (Cooke) Sacc. FF-FUS 2%ME Air 26 M 

250 Fusarium poae (Peck) Wollenw. BGA FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

251 Fusarium poae (Peck) Wollenw. BGB FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

252 Fusarium proliferatum var proliferatum (Matsush.) Nirenberg BGA FF-AIR, FOOD, FUS 2%ME Air 26 M 

(teleo. Gibberella intermedia) 

253 Fusarium proliferatum var proliferatum (Matsush.) Nirenberg BGB FF-AIR, FOOD, FUS 2%ME Air 26 M 

(teleo. Gibberella intermedia) 

254 Fusarium pseudoanthophilum Nirenberg, O’Donnell & Mabatanhema FF-FUS 2%ME Air 26 M 

255 Fusarium pseudograminearum Aoki & O’Donnell FF-FUS 2%ME Air 26 M 

(teleo. Gibberella coronicola) 

256 Fusarium ramigenum O’Donnell & Nirenberg FF-FUS 2%ME Air 26 M 

257 Fusarium redolens Wollenw. FF-FUS 2%ME Air 26 M 

258 Fusarium reticulatum Mont. (teleo. Gibberella cyanea) FF-FUS 2%ME Air 26 M 

259 Fusarium robustum Gerlach FF-FUS 2%ME Air 26 M 

260 Fusarium sacchari (Butler) W. Gams BGA FF-FOOD, FUS 2%ME Air 26 M 

261 Fusarium sacchari (Butler) W. Gams BGB FF-FOOD, FUS 2%ME Air 26 M 

262 Fusarium sambucinum var sambucinum Fuckel (teleo. Gibberella pulicaris) FF-FOOD, FUS 2%ME Air 26 Mm 

263 Fusarium solani (Mart.) Sacc. BGA FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

264 Fusarium solani (Mart.) Sacc. BGB FF-AIR, FOOD, FUS 2%ME Air 26 Mm 

265 Fusarium sporotrichioides var minus Sherb. BGA FF-AIR, FUS 2%ME Air 26 Mm 

266 Fusarium sporotrichioides var minus Sherb. BGB FF-AIR, FUS 2%ME Air 26 Mm 

267 Fusarium sporotrichioides var sporotrichioides Sherb. BGA FF-AIR, FUS 2%ME Air 26 Mm 

268 Fusarium sporotrichioides var sporotrichioides Sherb. BGB FF-AIR, FUS 2%ME Air 26 Mm 

269 Fusarium stilboides Wollenw. BGA (teleo. Gibberella stilboides) FF-FOOD,FUS 2%ME Air 26 Mm 

270 Fusarium stilboides Wollenw. BGB (teleo. Gibberella stilboides) FF-FOOD,FUS 2%ME Air 26 Mm 

271 Fusarium stilboides Wollenw. BGC (teleo. Gibberella stilboides) FF-FOOD,FUS 2%ME Air 26 Mm 

272 Fusarium subglutinans (Wollenw. & Reinking) P.E. Nelson et al. FF-AIR, FOOD, FUS 2%ME Air 26 M 

(teleo. Gibberella subglutinans) 

273 Fusarium thapsinum Klittich, Leslie, Nelson & Marasas FF-FUS 2%ME Air 26 M 

(teleo. Gibberella thapsinum) 

274 Fusarium torulosum (Berk. & Curt.) Nirenberg FF-FUS 2%ME Air 26 M 

275 Fusarium tricinctum (Corda) Sacc. BGA FF-AIR, FOOD, FUS 2%ME Air 26 M 

276 Fusarium tricinctum (Corda) Sacc. BGB FF-AIR, FOOD, FUS 2%ME Air 26 M 

277 Fusarium tricinctum (Corda) Sacc. BGC FF-AIR, FOOD, FUS 2%ME Air 26 M 

278 Fusarium tricinctum (Corda) Sacc. BGD FF-AIR, FOOD, FUS 2%ME Air 26 M 

279 Fusarium tumidum Scherbakoff (teleo. Gibberella tumida) FF-FUS 2%ME Air 26 M 

280 Fusarium udum E. Butler FF-FUS 2%ME Air 26 M 

281 Fusarium venenatum Nirenberg FF-FUS 2%ME Air 26 M 

282 Fusarium ventricosum Appel & Wollenw. FF-FUS 2%ME Air 26 M 

283 Fusarium verticillioides (Sacc.) Nirenberg BGA FF-AIR, FOOD, FUS 2%ME Air 26 M 

(teleo. Gibberella moniliformis) 

284 Fusarium verticillioides (Sacc.) Nirenberg BGB FF-AIR, FOOD, FUS 2%ME Air 26 M 

(teleo. Gibberella moniliformis) 

285 Fusarium xylarioides Steyaert (teleo. Gibberella xylarioides) FF-FUS 2%ME Air 26 M 

286 Galactomyces geotrichum(SS)(Butler & Perterson) Redhead & Malloch BGA FF-AIR, FOOD, YST 2%ME Air 26 M 

(anam. Geotrichum candidum) 

287 Galactomyces geotrichum(SS)(Butler & Perterson) Redhead & Malloch BGB FF-AIR, FOOD, YST 2%ME Air 26 M 


288 Galactomyces geotrichum(SS)(Butler & Perterson) Redhead & Malloch BGC FF-AIR, FOOD, YST 2%ME Air 26 M 


289 Galactomyces geotrichum(SS)(Butler & Perterson) Redhead & Malloch BGD FF-AIR, FOOD, YST 2%ME Air 26 M 


290 Geomyces pannorum var pannorum (Link) Sigler & Carmichael BGA FF-AIR 2%ME Air 26 M 

291 Geomyces pannorum var pannorum (Link) Sigler & Carmichael BGB FF-AIR 2%ME Air 26 M 

292 Geomyces pulvereus Hocking & Pitt FF-AIR 2%ME Air 26 

293 Geosmithia putterilli (Thom) Pitt FF-FOOD, PEN 2%ME Air 26 M 

294 Geotrichum klebahnii (Stautz) Morenz FF-AIR 2%ME Air 26 

295 Geotrichum sericeum (Stautz) de Hoog et al. FF-YST 2%ME Air 26 

296 Gibberella subglutinans (Edwards) Nelson, Toussoun & Marasas FF-FUS 2%ME Air 26 

(anam. Fusarium subglutinans) 

297 Gliocladium catenulatum Gilman & Abbott FF-AIR 2%ME Air 26 M 

298 Gliocladium viride Matruchot FF-AIR 2%ME Air 26 Mm 

299 Gonatobotrys simplex Corda FF-AIR 2%ME Air 26 M 

300 Hanseniaspora uvarum (Niehaus) El-Taby Shehata et al. FF-FOOD, YST 2%ME Air 26 Mm 

(anam. Kloekera apiculata) 






301 Hanseniaspora valbyensis Kloecker (anam. Kloeckera japonica) FF-FOOD, YST 2%ME Air 26 Mm 

302 Hemicarpenteles paradoxus Sarbhoy & Elphick (anam. Aspergillus paradoxus) FF-ASP 2%ME Air 26 M 

303 Hortaea werneckii (Horta) Nishimura & Miyaji FF-FOOD 2%ME Air 26 

304 Hyphopichia burtonii (Boidin et al.) Arx & Van der Walt FF-FOOD, YST 2%ME Air 26 Mm 

(anam. Candida chodatii) 

305 Hypocrea andinensis Samuels & O. Petrini FF-TRI 2%ME Air 26 M 

306 Hypocrea aurantia P. Hennings FF-TRI 2%ME Air 26 M 

307 Hypocrea aureoviridis Plowright &Cooke (anam. Trichoderma aureoviride) FF-TRI 2%ME Air 26 

308 Hypocrea gelatinosa (Tode: Fr.) Fr. FF-TRI 2%ME Air 26 M 

309 Hypocrea jecorina Berk. & Broome BGA (anam. Trichoderma reesei) FF-TRI 2%ME Air 26 M 

310 Hypocrea jecorina Berk. & Broome BGB (anam. Trichoderma reesei) FF-TRI 2%ME Air 26 M 

311 Hypocrea nigricans (Imai) Doi FF-TRI 2%ME Air 26 M 

312 Hypocrea novaezelandiae Samuels & O. Petrini FF-TRI 2%ME Air 26 M 

313 Hypocrea orientalis Samuels & O. Petrini FF-TRI 2%ME Air 26 M 

314 Hypocrea pseudokoningii Samuels & O. Petrini FF-TRI 2%ME Air 26 M 

315 Hypocrea schweinitizii (Fr.) Saccardo (anam. Trichoderma citrinoviride) FF-TRI 2%ME Air 26 M 

316 Issatchenkia orientalis Kudryavtsev (anam. Candida krusei) FF-FOOD, YST 2%ME Air 26 Mm 

317 Issatchenkia scutulata (Phaff et al.) Kurtzman et al. FF-YST 2%ME Air 26 

318 Kluyveromyces lactis var lactis (Dombrowski) Van der Walt FF-FOOD, YST 2%ME Air 26 Mm 

319 (anam. Candida sphaerica) 

320 Kluyveromyces marxianus (Hansen) Van.der Walt BGA FF-FOOD, YST 2%ME Air 26 Mm 

(anam. Candida kefyr) 

321 Kluyveromyces marxianus (Hansen) Van.der Walt BGB FF-FOOD, YST 2%ME Air 26 Mm 


322 Kluyveromyces marxianus (Hansen) Van.der Walt BGC FF-FOOD, YST 2%ME Air 26 Mm 


323 Kluyveromyces marxianus (Hansen) Van.der Walt BGD FF-FOOD, YST 2%ME Air 26 Mm 


324 Kluyveromyces marxianus (Hansen) Van.der Walt BGE FF-FOOD, YST 2%ME Air 26 Mm 


325 Lecythophora hoffmannii (van.Beyma) W. Gams BGA FF-FOOD 2%ME Air 26 M 

326 Lecythophora hoffmannii (van.Beyma) W. Gams BGB FF-FOOD 2%ME Air 26 M 

327 Lecythophora mutabilis (van Beyma) W. Gams FF-AIR 2%ME Air 26 M 

328 Mariannaea elegans var elegans (Corda) G. Amand ex Samson FF-AIR 2%ME Air 26 Mm 

329 Mariannaea elegans var punicea Samson FF-AIR 2%ME Air 26 M 

330 Microdochium bolleyi (Sprague) de Hoog & Herm.-Nijhof FF-AIR 2%ME Air 26 M 

331 Microsphaeropsis olivacea (Bonorden) v. Hoehnel FF-AIR 2%ME Air 26 M 

332 Monascus ruber v. Tiegham FF-AIR, FOOD 2%ME Air 26 M 

333 Moniliella acetoabutens Stolk & Dakin FF-AIR, FOOD 2%ME Air 26 Mm 

334 Moniliella suaveolens var suaveolens (Lindner) v.Arx FF-AIR, FOOD 2%ME Air 26 M 

335 Mucor circinelloides f sp circinelloides v. Tiegham FF-AIR, FOOD 2%ME Air 26 Mm 

336 Mucor hiemalis f sp hiemalis Wehmer FF-AIR, FOOD 2%ME Air 26 Mm 

337 Mucor mucedo Linn.: Fr. FF-AIR 2%ME Air 26 M 

338 Mucor plumbeus Bonord. FF-AIR, FOOD 2%ME Air 26 M 

339 Mucor racemosus f sp racemosus Fresen. FF-AIR, FOOD 2%ME Air 26 M 

340 Myceliophthora lutea Costantin FF-AIR 2%ME Air 26 

341 Myrothecium roridum Tode:Fr. FF-AIR 2%ME Air 26 M 

342 Myrothecium verrucaria (Alb. & Schwein.:Fr.) Ditmar FF-AIR 2%ME Air 26 M 

343 Nectria aureofulva Cooke & Ellis (anam. Clonostachys sp.) FF-FUS 2%ME Air 26 M 

344 Nectria hematococca Berk. & Br. (anam. Fusarium eumartii) FF-FUS 2%ME Air 26 

345 Nectria ochroleuca (Schweinitz) Berkeley FF-FUS 2%ME Air 26 

346 Nectria sesquicilli Samuels (anam. Clonostachys sp.) FF-FUS 2%ME Air 26 M 

347 Neosartorya fischeri var fischeri (Wehmer) Malloch & Cain FF-AIR, FOOD, ASP 2%ME Air 26 M 

(anam. Aspergillus .fischerianus) 

348 Neurospora crassa Schear & Dodge FF-AIR, FOOD 2%ME Air 26 M 

349 Neurospora sitophilia Schear & Dodge FF-AIR, FOOD 2%ME Air 26 Mm 

350 Nigrospora oryzae (Berk. & Broome) Petch FF-AIR, FOOD 2%ME Air 26 Mm 

351 Nigrospora sphaerica (sacc.) Mason FF-AIR, FOOD 2%ME Air 26 M 

352 Ophiostoma piceae (Munch) H. & P. Sydow BGA FF-AIR 2%ME Air 26 Mm 

353 Ophiostoma piceae (Munch) H. & P. Sydow BGB FF-AIR 2%ME Air 26 Mm 

354 Ophiostoma ulmi (Buisman) Nannfeldt FF-AIR 2%ME Air 26 M 

355 Paecilomyces carneus (Duché & Heim) A. Brown & G. Smith FF-AIR 2%ME Air 26 M 

356 Paecilomyces farinosus (Holm:Fr.) A. Brown & G. Smith FF-AIR 2%ME Air 26 M 

357 Paecilomyces fumosoroseus (Wize) A. Brown & G. Smith FF-AIR 2%ME Air 26 M 

358 Paecilomyces lilacinus (Thom) Samson FF-AIR, FOOD 2%ME Air 26 M 

359 Paecilomyces marquandii (Massee) S. Hughes FF-AIR 2%ME Air 26 M 

360 Paecilomyces variotii (Bainer) BGA FF-AIR, FOOD 2%ME Air 26 Mm 






361 Paecilomyces variotii (Bainer) BGB FF-AIR, FOOD 2%ME Air 26 Mm 

362 Penicillium adametzii Zaleski BGA FF-PEN 2%ME Air 26 Mm 

363 Penicillium adametzii Zaleski BGB FF-PEN 2%ME Air 26 Mm 

364 Penicillium aethiopicum Frisvad FF-FOOD, PEN 2%ME Air 26 M 

365 Penicillium allii Vincent & Pitt FF-FOOD, PEN 2%ME Air 26 Mm 

366 Penicillium argillaceum Stolk et al. FF-AIR, PEN 2%ME Air 26 Mm 

367 Penicillium atramentosum Thom FF-PEN 2%ME Air 26 M 

368 Penicillium aurantiogriseum Dierckx BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

369 Penicillium aurantiogriseum Dierckx BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

370 Penicillium aurantiovirens Biourge FF-PEN 2%ME Air 26 Mm 

371 Penicillium bilaiae Chalabuda FF-AIR, PEN 2%ME Air 26 Mm 

372 Penicillium brevicompactum Dierckx BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

373 Penicillium brevicompactum Dierckx BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

374 Penicillium brevicompactum Dierckx BGC FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

375 Penicillium camemberti Thom BGA FF-FOOD, PEN 2%ME Air 26 Mm 

376 Penicillium camemberti Thom BGB FF-FOOD, PEN 2%ME Air 26 Mm 

377 Penicillium canescens Sopp FF-PEN 2%ME Air 26 Mm 

378 Penicillium capsulatum Raper & Fennell FF-AIR, PEN 2%ME Air 26 Mm 

379 Penicillium chermesinum Biourge FF-AIR, PEN 2%ME Air 26 Mm 

380 Penicillium chrysogenum Thom FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

381 Penicillium citreonigrum Dierckx BGA (teleo. Eupenicillium euglaucum) FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

382 Penicillium citreonigrum Dierckx BGB (teleo. Eupenicillium euglaucum) FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

383 Penicillium citreonigrum Dierckx BGC (teleo. Eupenicillium euglaucum) FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

384 Penicillium citrinum Thom FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

385 Penicillium clavigerum Demelius FF-PEN 2%ME Air 26 M 

386 Penicillium commune Thom BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

387 Penicillium commune Thom BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

388 Penicillium corylophilum Dierckx FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

389 Penicillium crateriforme Gilman & Abbott FF-PEN 2%ME Air 26 

390 Penicillium crustosum Thom FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

391 Penicillium cyclopium Westling FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

392 Penicillium decumbens Thom (teleo. Eupenicillium meridianum) FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

393 Penicillium digitatum (Pers.:Fr.) Sacc. BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

394 Penicillium digitatum (Pers.:Fr.) Sacc. BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

395 Penicillium discolor Frisvad & Samson FF-PEN 2%ME Air 26 M 

396 Penicillium duclauxii Delucr. FF-PEN 2%ME Air 26 M 

397 Penicillium echinulatum Fassatiova FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

398 Penicillium erythromellis Hocking FF-PEN 2%ME Air 26 Mm 

399 Penicillium expansum Link BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

400 Penicillium expansum Link BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

401 Penicillium expansum Link BGC FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

402 Penicillium expansum Link BGD FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

403 Penicillium fellutanum Biourge FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

404 Penicillium freii Frisvad & Samson BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

405 Penicillium freii Frisvad & Samson BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

406 Penicillium freii Frisvad & Samson BGC FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

407 Penicillium funiculosum Thom BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

408 Penicillium funiculosum Thom BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

409 Penicillium glabrum (Wehmer) Westling FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

410 Penicillium glandicola var glandicola (Oud.) Seifert & Samson FF-PEN 2%ME Air 26 M 

411 Penicillium griseofulvum Dierckx FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

412 Penicillium herquei Bainier & Sartory FF-AIR, PEN 2%ME Air 26 Mm 

413 Penicillium hirsutum Dierckx FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

414 Penicillium hordei Stolk FF-AIR, FOOD, PEN 2%ME Air 26 M 

415 Penicillium implicatum Biourge FF-AIR, FOOD, PEN 2%ME Air 26 m 

416 Penicillium islandicum Sopp FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

417 Penicillium italicum Wehmer FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

418 Penicillium janczewskii K.M. Zaleski FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

419 Penicillium janthienellum Biourge FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

420 Penicillium jansenii Zaleski FF-PEN 2%ME Air 26 M 

421 Penicillium lagena (Delitsch) Stolk & Samson FF-PEN 2%ME Air 26 Mm 

422 Penicillium lanosum Westling FF-PEN 2%ME Air 26 Mm 

423 Penicillium lividum Westling FF-PEN 2%ME Air 26 M 

424 Penicillium madriti G.Smith FF-PEN 2%ME Air 26 M 

425 Penicillium melanoconidium (Frisvad) Frisvad & Samson BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

426 Penicillium melanoconidium (Frisvad) Frisvad & Samson BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

427 Penicillium melinii Thom FF-PEN 2%ME Air 26 Mm 






428 Penicillium miczynski Zaleski FF-AIR, PEN 2%ME Air 26 Mm 

429 Penicillium minioluteum Dierckx BGA FF-AIR, PEN 2%ME Air 26 Mm 

430 Penicillium minioluteum Dierckx BGB FF-AIR, PEN 2%ME Air 26 Mm 

431 Penicillium minioluteum Dierckx BGC FF-AIR, PEN 2%ME Air 26 Mm 

432 Penicillium montanense M. Christensen & Backus FF-PEN 2%ME Air 26 M 

433 Penicillium nalgiovense Laxa FF-FOOD, PEN 2%ME Air 26 M 

434 Penicillium neoechinulatum (Frisvad et al.) Frisvad & Samson FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

435 Penicillium oblatum Pitt & Hocking FF-PEN 2%ME Air 26 Mm 

436 Penicillium ochrochloron Biourge BGA FF-AIR, PEN 2%ME Air 26 Mm 

437 Penicillium ochrochloron Biourge BGB FF-AIR, PEN 2%ME Air 26 Mm 

438 Penicillium olsonii Bainier & Sartory BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

439 Penicillium olsonii Bainier & Sartory BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

440 Penicillium oxalicum Currie & Thom FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

441 Penicillium paxilli Bainier FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

442 Penicillium phoeniceum van.Beyma FF-AIR, PEN 2%ME Air 26 M 

443 Penicillium piceum Raper & Fennell FF-AIR, PEN 2%ME Air 26 M 

444 Penicillium pinophilum Hedge. BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

445 Penicillium pinophilum Hedge. BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

446 Penicillium pinophilum Hedge. BGC FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

447 Penicillium pinophilum Hedge. BGD FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

448 Penicillium polonicum Zaleski BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

449 Penicillium polonicum Zaleski BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

450 Penicillium primulinum Pitt FF-PEN 2%ME Air 26 Mm 

451 Penicillium purpurescens (Sopp.) Biourge FF-PEN 2%ME Air 26 M 

452 Penicillium purpurogenum Stoll BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

453 Penicillium purpurogenum Stoll BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

454 Penicillium purpurogenum Stoll BGC FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

455 Penicillium purpurogenum var rubisclorotium Thom FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

456 Penicillium raistrickii G.Smith FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

457 Penicillium restrictum J.C. Gilman & E.V. Abbott BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

458 Penicillium restrictum J.C. Gilman & E.V. Abbott BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

459 Penicillium roqueforti Thom BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

460 Penicillium roqueforti Thom BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

461 Penicillium roqueforti Thom BGC FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

462 Penicillium roqueforti Thom BGD FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

463 Penicillium roqueforti Thom BGE FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

464 Penicillium rubrum Stoll BGA FF-AIR, PEN 2%ME Air 26 M 

465 Penicillium rubrum Stoll BGB FF-AIR, PEN 2%ME Air 26 M 

466 Penicillium rugulosum Thom BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

467 Penicillium rugulosum Thom BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

468 Penicillium rugulosum Thom BGC FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

469 Penicillium sclerotiorum Van.Beyma FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

470 Penicillium siamense Manoch & Ramirez FF- PEN 2%ME Air 26 Mm 

471 Penicillium simplicissimum (Oudem.) Thom BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

472 Penicillium simplicissimum (Oudem.) Thom BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

473 Penicillium smithii Quintanilla FF-PEN 2%ME Air 26 M 

474 Penicillium solitum Westling BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

475 Penicillium solitum Westling BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

476 Penicillium solitum Westling BGC FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

477 Penicillium steckii Zaleski BGA FF-AIR, PEN 2%ME Air 26 m 

478 Penicillium steckii Zaleski BGB FF-AIR, PEN 2%ME Air 26 m 

479 Penicillium thomii Maire BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

480 Penicillium thomii Maire BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

481 Penicillium tricolor Fresvad et al. FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

482 Penicillium trubatum Westling BGA (teleo. Eupenicillium baarnese) FF-PEN 2%ME Air 26 Mm 

483 Penicillium trubatum Westling BGB (teleo. Eupenicillium baarnese) FF-PEN 2%ME Air 26 Mm 

484 Penicillium variabile Sopp BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

485 Penicillium variabile Sopp BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

486 Penicillium verrucosum var verrucosum Dierckx BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

487 Penicillium verrucosum var verrucosum Dierckx BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

488 Penicillium viridicatum Westling BGA FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

489 Penicillium viridicatum Westling BGB FF-AIR, FOOD, PEN 2%ME Air 26 Mm 

490 Penicillium vulpinum (Cooke & Massee) Seifert & Samson BGA FF-AIR, PEN 2%ME Air 26 Mm 

491 Penicillium vulpinum (Cooke & Massee) Seifert & Samson BGB FF-AIR, PEN 2%ME Air 26 Mm 

492 Pestalotiopsis maculans (Corda) Nag Raj FF-AIR 2%ME Air 26 

493 Petromyces alliaceus Malloch & Cain BGA (anam. Aspergillus alliaceous) FF-AIR, FOOD, ASP 2%ME Air 26 M 

494 Petromyces alliaceus Malloch & Cain BGB (anam. Aspergillus alliaceous) FF-AIR, FOOD, ASP 2%ME Air 26 M 






495 Phanerochaete chrysosporium Burdsall FF-FOOD 2%ME Air 26 M 

496 Phialophora fastigiata (Lagerb. & Melin) Conant FF-AIR, FOOD 2%ME Air 26 

497 Phialophora malorum (Kidd & Beaumont) McColloch FF-AIR 2%ME Air 26 M 

498 Phialophora richardsiae (Nannf. & Melin) Conant FF-AIR 2%ME Air 26 M 

499 Phoma chrysanthemicola Hollos FF-AIR 2%ME Air 26 

500 Phoma exigua var exigua Desm. FF-AIR 2%ME Air 26 M 

501 Phoma glomerata (Corda) Wollenw. & Hochapfel BGA FF-AIR, FOOD 2%ME Air 26 M 

502 Phoma glomerata (Corda) Wollenw. & Hochapfel BGB FF-AIR, FOOD 2%ME Air 26 M 

503 Phoma herbarum Westend. FF-AIR 2%ME Air 26 M 

504 Phoma macrostoma var macrostoma Mont. FF-AIR 2%ME Air 26 M 

505 Phoma multirostrata (Mathur et al.) Dorenbosch & Boerema FF-AIR 2%ME Air 26 

506 Phoma septicidalis Boerema FF-AIR 2%ME Air 26 M 

507 Phoma sorghina (Sacc.) Boerema FF-AIR, FOOD 2%ME Air 26 M 

508 Pichia anomala (Sydow & Sydow) Kurtzman BGA FF-FOOD, YST 2%ME Air 26 Mm 

(anam. Candida pelliculosa) 

509 Pichia anomala (Sydow & Sydow) Kurtzman BGB FF-FOOD, YST 2%ME Air 26 Mm 

(anam. Candida pelliculosa) 

510 Pichia fermentans Lodder BGA (anam. Candida lambica) FF-FOOD, YST 2%ME Air 26 M 

511 Pichia fermentans Lodder BGB (anam. Candida lambica) FF-FOOD, YST 2%ME Air 26 M 

512 Pichia guilliermondii Wickernam (anam. Candida guilliermondii) FF-FOOD, YST 2%ME Air 26 Mm 

513 Pichia haplophila Shifrine & Phaff FF-YST 2%ME Air 26 

514 Pichia membranifaciens Hansen (anam. Candida valida) FF-FOOD, YST 2%ME Air 26 Mm 

515 Pichia pastoris (Guilliermond) Phaff FF-YST 2%ME Air 26 

516 Pichia petersonii (Wickerham) Kurtzman FF-YST 2%ME Air 26 

517 Pichia spartinae Ahearn et al. FF-YST 2%ME Air 26 

518 Pithomyces chartarum (Berk. & Curtis) M.B. Ellis FF-AIR 2%ME Air 26 

519 Pithomyces sacchari (Speg.) M.B. Ellis FF-AIR 2%ME Air 26 M 

520 Rhinocladiella atrovirens Nannf. BGA FF-AIR 2%ME Air 26 M 

521 Rhinocladiella atrovirens Nannf. BGB FF-AIR 2%ME Air 26 M 

522 Rhizomucor pusillus (Lindt) Schipper FF-AIR, FOOD 2%ME Air 26 M 

523 Rhizopus microsporus var microsporus v. Tiegh FF-AIR, FOOD 2%ME Air 26 M 

524 Rhizopus oligosporus Saito FF-AIR, FOOD 2%ME Air 26 Mm 

525 Rhizopus oryzae Went & Prins. Geerl. FF-AIR, FOOD 2%ME Air 26 Mm 

526 Rhizopus sexualis var sexualis (G. Sm.) Callen FF-AIR, FOOD 2%ME Air 26 M 

527 Rhizopus stolonifer var stolonifer (EHRENB.:FR.) LINDNER BGA FF-AIR, FOOD 2%ME AIR 26 Mm 

528 Rhizopus stolonifer var stolonifer (Ehrenb.:Fr.) Lindner BGB FF-AIR, FOOD 2%ME Air 26 Mm 

529 Rhodotorula aurantica (Saito) Lodder FF-AIR, YST 2%ME Air 26 Mm 

530 Rhodotorula minuta (Saito) F.C. Harrison FF-YST 2%ME Air 26 

531 Rhodotorula mucilaginosa(AS) (A.Jörg) F.C. Harrison BGA FF-AIR, FOOD, YST 2%ME Air 26 M 

532 Rhodotorula mucilaginosa (AS) (A.Jörg) F.C. Harrison BGB FF-AIR, FOOD, YST 2%ME Air 26 M 

533 Rhodotorula muscorum (di Menna) von Arx & Weijman FF-YST 2%ME Air 26 

534 Saccharomyces bayanas Sacc. FF-FOOD, YST 2%ME Air 26 M 

535 Saccharomyces cerevisiae Meyen ex E.C. Hanson BGA FF-FOOD, YST 2%ME Air 26 M 

(anam. Candida robusta) 

536 Saccharomyces cerevisiae Meyen ex E.C. Hanson BGB FF-FOOD, YST 2%ME Air 26 M 


537 Saccharomyces cerevisiae Meyen ex E.C. Hanson BGC FF-FOOD, YST 2%ME Air 26 M 


538 Saccharomyces cerevisiae Meyen ex E.C. Hanson BGD FF-FOOD, YST 2%ME Air 26 M 


539 Saccharomyces cerevisiae Meyen ex E.C. Hanson BGE FF-FOOD, YST 2%ME Air 26 M 


540 Saccharomyces cerevisiae Meyen ex E.C. Hanson BGF FF-FOOD, YST 2%ME Air 26 M 


541 Saccharomyces cerevisiae Meyen ex E.C. Hanson BGG FF-FOOD, YST 2%ME Air 26 M 


542 Saccharomyces exiguus(SS) Reess ex Hanson (anam. Candida holmii) FF-FOOD, YST 2%ME Air 26 Mm 

543 Schizosaccharomyces octosporus Beijerinck (Octosporomyces octosporus) FF-FOOD, YST 2%ME Air 26 m 

544 Schizosaccharomyces pombe Lindler FF-FOOD, YST 2%ME Air 26 Mm 

545 Scopulariopsis asperula (Sacc.) Hughes FF-AIR 2%ME Air 26 M 

546 Scopulariopsis brevicaulis (Sacc.) Bainier BGA FF-AIR, FOOD 2%ME Air 26 Mm 

547 Scopulariopsis brevicaulis (Sacc.) Bainier BGB FF-AIR, FOOD 2%ME Air 26 Mm 

548 Scopulariopsis brumptii Salvanet-Duval FF-AIR 2%ME Air 26 M 

549 Scopulariopsis candida (Guéguen) Vuill. FF-AIR, FOOD 2%ME Air 26 Mm 

550 Scopulariopsis chartarum (G. Smith) Morton & G. Smith BGA FF-AIR 2%ME Air 26 M 

551 Scopulariopsis chartarum (G. Smith) Morton & G. Smith BGB FF-AIR 2%ME Air 26 M 

552 Scopulariopsis fusca Zach BGA FF-FOOD 2%ME Air 26 Mm 






553 Scopulariopsis fusca Zach BGB FF-FOOD 2%ME Air 26 Mm 

554 Sistotrema brinkmannii (Bresadola) John Eriksson FF-AIR 2%ME Air 26 

551 Sistotrema raduloides (Karsten) Donk FF-AIR 2%ME Air 26 

552 Sporobolomyces roseus Kluyver & Van Niel FF-AIR, YST 2%ME Air 26 M 

553 Sporopachydermia ceriana Rodrigues de Miranda FF-YST 2%ME Air 26 

554 Sporothrix schenckii var schenckii Kektoen & Perkins FF-AIR 2%ME Air 26 M 

555 Stachybotrys bisbyi (Srinavesan) Barron FF-AIR 2%ME Air 26 

556 Stachybotrys chartarum (Ehrenb.) Hughes FF-AIR, FOOD 2%ME Air 26 M 

557 Stachybotrys cylindrospora C.N. Jensen FF-AIR 2%ME Air 26 Mm 

558 Stachybotrys echinulata (Rivolta) G. Smith FF-AIR 2%ME Air 26 M 

559 Sterigmatomyces elviae Sonck & Yarrow FF-AIR, FOOD, YST 2%ME Air 26 

560 Sterigmatomyces halophilus Fell FF-AIR, YST 2%ME Air 26 

561 Syncephalastrum racemosum J.Schrot. FF-AIR, FOOD 2%ME Air 26 Mm 

562 Talaromyces bacillisporus (Swift) C.R. Benjamin FF-AIR, FOOD, PEN 2%ME Air 26 M 

(anam. Penicillium bacillisporum) 

563 Talaromyces flavus var flavus (Klocker) Stolk & Samson FF-AIR, FOOD, PEN 2%ME Air 26 M 

(anam. Penicillium vermiculatum) 

564 Talaromyces macrosporus (Stolk & Samson) Frisvad et al. FF-FOOD, PEN 2%ME Air 26 Mm 

(anam. Penicillium dangesrdii) 

565 Thamnidium elegans Link FF-AIR, FOOD 2%ME Air 26 M 

566 Tilletiopsis albescens Gokhale FF-AIR 2%ME Air 26 M 

567 Torula herbarum Link: Fr. FF-AIR 2%ME Air 26 M 

568 Torulaspora delbrueckii (Lindner) Lindner (anam. Candida colliculosa) FF-FOOD, YST 2%ME Air 26 Mm 

569 Trichoderma asperellum FF-AIR, FOOD, TRI 2%ME Air 26 M 

570 Trichoderma atroviride Karsten FF-AIR, FOOD, TRI 2%ME Air 26 Mm 

571 Trichoderma aureoviride Rifai (teleo. Hypocrea aureoviridis) FF-TRI 2%ME Air 26 Mm 

572 Trichoderma citrinovirde Bissett BGA (teleo. Hypocrea schweinitzii) FF-AIR, FOOD, TRI 2%ME Air 26 Mm 

573 Trichoderma citrinovirde Bissett BGB (teleo. Hypocrea schweinitzii) FF-AIR, FOOD, TRI 2%ME Air 26 Mm 

574 Trichoderma citrinovirde Bissett BGC (teleo. Hypocrea schweinitzii) FF-AIR, FOOD, TRI 2%ME Air 26 Mm 

575 Trichoderma crassum Bissett FF-TRI 2%ME Air 26 M 

576 Trichoderma fasiculatum Bissett FF-TRI 2%ME Air 26 M 

577 Trichoderma fertile Bissett FF-TRI 2%ME Air 26 M 

578 Trichoderma ghanense Y. Doi, Y. Abe & J. Sugiyama FF-TRI 2%ME Air 26 M 

579 Trichoderma hamatum (Bon.) Bainier FF-AIR, TRI 2%ME Air 26 M 

580 Trichoderma harzianum Rifai BGA FF-AIR, FOOD, TRI 2%ME Air 26 Mm 

581 Trichoderma harzianum Rifai BGB FF-AIR, FOOD, TRI 2%ME Air 26 Mm 

582 Trichoderma harzianum Rifai BGC FF-AIR, FOOD, TRI 2%ME Air 26 Mm 

583 Trichoderma koningii Oud. (teleo. Hypocrea koningii) FF-AIR, TRI 2%ME Air 26 Mm 

584 Trichoderma longibrachiatum Rifai BGA FF-AIR, TRI 2%ME Air 26 Mm 

585 Trichoderma longibrachiatum Rifai BGB FF-AIR, TRI 2%ME Air 26 Mm 

586 Trichoderma longibrachiatum Rifai BGC FF-AIR, TRI 2%ME Air 26 Mm 

587 Trichoderma longibrachiatum Rifai BGD FF-AIR, TRI 2%ME Air 26 Mm 

588 Trichoderma minutisporum Bissett FF-AIR, TRI 2%ME Air 26 M 

589 Trichoderma oblongisporum Bissett FF-TRI 2%ME Air 26 

590 Trichoderma piluiliferum Webster & Rifai (teleo. Hypocrea pilulifera) FF-TRI 2%ME Air 26 M 

591 Trichoderma polysporum (Link:Fr.) Rifai FF-AIR, TRI 2%ME Air 26 M 

592 Trichoderma pseudokoningii Rifai (teleo. Hypocrea pseudokoningii) FF-TRI 2%ME Air 26 Mm 

593 Trichoderma reesei Simmons BGA (teleo. Hypocrea jecorina) FF-TRI 2%ME Air 26 Mm 

594 Trichoderma reesei Simmons BGB (teleo. Hypocrea jecorina) FF-TRI 2%ME Air 26 Mm 

595 Trichoderma saturnisporum Hammill FF-TRI 2%ME Air 26 Mm 

596 Trichoderma spirale Bissett FF-TRI 2%ME Air 26 M 

597 Trichoderma strictipile Bissett FF-TRI 2%ME Air 26 M 

598 Trichoderma strigosum Bissett FF-TRI 2%ME Air 26 M 

599 Trichoderma tomentosum Bissett FF-TRI 2%ME Air 26 M 

600 Trichoderma virens (Miller et al.)v.Arx BGA FF-TRI 2%ME Air 26 Mm 

601 Trichoderma virens (Miller et al.)v.Arx BGB FF-TRI 2%ME Air 26 Mm 

602 Trichoderma viride Pers.:Fr. FF-AIR, FOOD, TRI 2%ME Air 26 Mm 

603 Trichothecium roseum (Pers.) Link FF-AIR, FOOD 2%ME Air 26 Mm 

604 Trichurus spiralis Hasselbring BGA FF-AIR 2%ME Air 26 M 

605 Trichurus spiralis Hasselbring BGB FF-AIR 2%ME Air 26 M 

606 Tritirachium oryzae (Vincens) de Hoog FF-AIR 2%ME Air 26 M 

607 Ulocladium atrum Preuss FF-AIR 2%ME Air 26 M 

608 Ulocladium botrytis Preuss BGA FF-AIR, FOOD 2%ME Air 26 Mm 

609 Ulocladium botrytis Preuss BGB FF-AIR, FOOD 2%ME Air 26 Mm 

610 Ulocladium chartarum (Preuss) Simmons FF-AIR, FOOD 2%ME Air 26 Mm 

611 Ustilago maydis (de Candolle) Corda FF-AIR, YST 2%ME Air 26 Mm 

612 Verticillium lecanii (Zimmermann) Viegas FF-AIR 2%ME Air 26 M 






613 Wallemia sebi (Fr.) v. Arx FF-AIR, FOOD 2%ME Air 26 M 

614 Williopsis saturnus var saturnus (Klocker) Zender FF-YST 2%ME Air 26 

615 Yarrowia lipolytica (SS) (Wickerham et al.) v.d. Walt & v. Arx FF-FOOD, YST 2%ME Air 26 Mm 

(anam. Candida lipolytica) 

616 Zygorhyncus moelleri Vuillemin FF-AIR 2%ME Air 26 

617 Zygosaccharomyces bailii (Lindner) Guillierm FF-FOOD, YST 2%ME Air 26 Mm 

618 Zygosaccharomyces rouxii (Boutroux) Yarrow FF-FOOD, YST 2%ME Air 26 Mm 

619 Zygosporium mycophilum (Vuillemin) Saccardo FF-AIR 2%ME Air 26 M 




Appendix 5: Program Printouts 


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Manual printout (sample) 



Manual Printout Key 

1. Software version 

2. Name of file 

3. Data file record Edit status: OK, Out, Hold, Atypical 

4. Time MicroPlate was read 

5. Time file was modified 

6. Does user have Unrestricted Access to software? 

7. Number of MicroPlate 

8. Sample identifiers 

9. Data generation mode (file) 

10. Number of positive, borderline, and negative reactions 

11. Name of database searched 

12. Positive (), borderline ({/}), and negative reactions (-). A negative (-) well with a plus (+) on the right 

side is a mismatch. The well read as negative, but at least 80% of the strains of that organism tested in our 

database were positive for that well. A plus (+) with a < on the left and a minus (–) on the right is a 

mismatch, but the well read as positive, while the strains in our database were mostly negative for that well. 

13. Species identification/ #1 identification 

14. #2-9 identifications 



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Reader printout (sample) 


Reader and Saved File Modes Printout Key 



2. If the printout is from a saved file, this entry lists file name, position of the entry in 

the file, and edit status. 

3. Threshold mode. This listing may say “Manual” or “Automatic.” In Automatic 

mode, the software automatically calculates a threshold cutoff for negative and 

positive reactions, indicated by numerical OD values. Any values between these two 

numbers are considered borderline. The threshold can change from MicroPlate to 

MicroPlate, depending on the intensity of the positive reactions and the clarity of the 

negative reactions. In Manual Threshold mode, the user has changed the threshold 

cutoffs to match the visual interpretation of the plate. 






9. Operating mode (file) 

10. Number of positive, borderline, and negative reactions 


12. Numbers seen in each well is the percent change data of the OD compared to well 

A1 (A1 is always 0). DWD (dual wavelength data) = (590nm-750nm)x – (590nm- 

750nm)A1 x 1000, where x = any well. Positive (), borderline ({}), and negative 

reactions (no brackets). Numbers with no brackets and a plus (+) on the right side are 

mismatches. The well read as negative, but at least 80% of the strains of that 

organism tested in our database were positive for that well. Numbers with a < on the 

left and a minus (–) on the right are also mismatches, but the well read as positive, 

while the strains in our database were mostly negative for that well. Numbers with a 

minus (-) to the left of the well value mean that the well’s OD was less than the OD 

of the control well. 

13. Species Iderntification/#1 identification 




Fungal printout (sample) 

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FF Printout Key 


2. If the printout is from a saved file, this entry lists file name, position of the entry in 

the file, and edit status. 

3. Threshold mode. This listing may say “Manual” or “Automatic.” In Automatic 

mode, the software automatically calculates a threshold cutoff for negative and 

positive reactions, indicated by numerical OD values. Any values between these two 

numbers are considered borderline. The threshold can change from MicroPlate to 

MicroPlate, depending on the intensity of the positive reactions and the clarity of the 

negative reactions. Two thresholds are given for the FF MicroPlate: color (for 490 

nm readings) and turbidity (for 750 nm readings). 

4. Filters and their positions 






10. Operating mode (file) 

11. Number of positive, borderline, and negative reactions and listed for color and 

turbidity 


13. OD data for each well (in 8x12 format) 

• For color data: The numbers seen in each well is the percent change data of the 

optical density of the difference between 490nm and 750 nm compared to the 

A1 control well (A1 will always be 0). The Dual Wavelength Data formula =. 

DWD = (490nm - 750nm)x - (490nm - 750nm)A1 x 1000, where x = any 

well. The turbidity is subtracted out to obtain the OD contributed by the color 

• For the Turbidity Data: The numbers seen in each well is the percent change 

data of the optical density of the 750 nm readings compared to the A1 control 

well (A1 will always be 0). The % Change formula = % Change 750nm = 

(750nmx - 750nmA1 ) x 1600, where x = any well* 

• Positive (), borderline ({}), and negative reactions (no brackets). Numbers 

with no brackets and a plus (+) on the right side are mismatches. The well read 

as negative, but at least 80% of the strains of that organism tested in our 

database were positive for that well. Numbers with a < on the left and a minus (– 

) on the right are also mismatches, but the well read as positive, while the strains 

in our database were mostly negative for that well. Numbers with a minus (-) to 

the left of the well value mean that the well’s OD was less than the OD of the 

control well. 

14. Species Identification/#1 identification 


*We use a larger multiplication factor for the turbidity to have the OD readings on the same scale as 490 readings. 



Print ranked data headers printout (sample) 


Print species counts (sample) 




Print species/plate dendrograms, page 1 (sample) 











Appendix 6: Dangerous Pathogens (DP) Database 

Appendix 6: Dangerous Pathogen (DP) 

Database 

Biolog makes a supplemental database for species falling into a category 

called Dangerous Pathogens (e.g., the organisms that generally require 

BL2-BL3 handling protocols). If your laboratory is testing for these 

pathogens, you will know in advance if any samples are suspected of 

containing these organisms. Follow the instructions in the body of this 

user guide. As appropriate, also follow the special procedures listed here. 

Safety Considerations 

To comply with government safety regulations 1 use Biosafety Level 2 

(BL2) methods, containment equipment, and facilities. Immunization of 

laboratory personnel may be required. It is recommended that Biosafety 

Level 3 (BL3) practices, containment equipment, and facilities are used 

for work involving production volumes or concentration of cultures, and 

for activities that have a high potential for aerosol production. In these 

facilities, immunization is recommended for all persons working with the 

agent, all persons working in the same laboratory room where the cultures 

are handled, and all persons working with infected animals. 

Special Procedures for Dangerous Pathogens 

For the most part, you will prepare, read, and interpret these samples 

exactly as described throughout this manual. There are, however, a few 

exceptions. Table 13-1 lists the special procedures required for dangerous 

pathogens. 

1 Biosafety in Microbiological and Biomedical Laboratories, U.S. Department of Health and Human Services, 

Centers for Disease Control and Prevention, and National Institutes of Health, Fourth Edition, May 1999. 


Appendix 6 Dangerous Pathogens (DP) Database 

Organism Special Procedures 

Francisella tularensis Set up as a Gram-negative Fastidious organism 

Do not add thioglycolate to inoculating fluid 

Yersinia pestis Set up as an Gram-negative Enteric organism 


Grow only on BUG + Blood (not TSA + Blood) 

Burkholderia mallei and 

Set up as a Gram-negative Non-Enteric organism 

Burkholderia pseudomallei 

Grow only on BUG + Blood (not TSA + Blood) 

Brucella mellitensis 

(Use this procedure for B. abortus, B. 

canis, B. suis and other species that have 

been reclassified as B. mellitensis.) 

TABLE 12-1: PROCEDURE EXCEPTIONS FOR DANGEROUS PATHOGENS 

Incubate at 30° C 

Set up as a Gram-negative Fastidious organism 


Multiple chocolate plates are needed to obtain proper inoculum

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