Abstract
Complete and/or partial DNA sequences for the plastid-encoded rbcL gene and the 5′ end of the mitochondrial cytochrome oxidase I (COI-5P) gene were used to re-examine the systematics of Hypnea species (Cystocloniaceae, Rhodophyta) from North Carolina, USA. These data, combined with light microscopic observations, indicate that two species (Hypnea cryptica and H. musciformis sensu stricto) are present in nearshore waters of coastal North Carolina. Molecular and morphological analyses with topotype material of Hypnea volubilis from North Carolina offshore waters revealed that it and Calliblepharis saidana are conspecific. Hypnea volubilis is proposed as a heterotypic synonym of C. saidana. This is the first report of Calliblepharis from the United States Atlantic coast and only the second report from the western hemisphere.
Funding source: National Science Foundation
Award Identifier / Grant number: DEB-0742437
Award Identifier / Grant number: DEB-0743334
Funding source: CMS DNA-Algal Trust
About the authors
Jason T. Campbell completed his Master of Science degree at the University of North Carolina at Wilmington in May 2021. His research focused on the phylogenetics of Hypnea species throughout the Western. He is currently working as a Fisheries Observer in the Gulf of Mexico Shrimp and Reef fish fishery out of Bayou la Batre, AL.
D. Wilson Freshwater is researcher at the Center for Marine Science, University of North Carolina Wilmington. His current research interests include molecular phylogeny and taxonomy of marine algae, marine floristics, Pomatomus saltatrix aggressive behavior, and ecology of marine hard bottom communities.
J. Craig Bailey is researcher at the Center for Marine Science, University of North Carolina Wilmington. His current research interests include the evolution stramenopiles and red algae. He is a failed humorist.
Acknowledgements
The authors wish to thank the Center for Marine Science’s Marine Operations division, the Coastal Oceans Research and Monitoring Program (CORMP) and late NOAA-NURC program for assistance with the collection of offshore specimens. We are especially indebted to captains and divers Steve Hall, Ken Johns, Dave Wells, Jay Souza, Chris LaClair, Brett Bolton, Brian Degan, Jennifer Idol, and Henry J. Styron. We also thank J. ‘Trey’ Foscue for laboratory assistance.
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: DWFs work on this project was funded by NC SeaGrant, US National Science Foundation Grants DEB-0742437 and DEB-0743334, and the CMS DNA-Algal Trust.
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Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
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Ethical standards: The authors declare that no laws were willfully violated in the course of this research, only marine algae.
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