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In vitro study of disinfectants on the embryonation and survival of Toxascaris leonina eggs

Published online by Cambridge University Press:  04 October 2017

Kh.M. El-Dakhly ,
A.S.M. Aboshinaf ,
W.M. Arafa ,
L.N. Mahrous ,
E. El-Nahass ,
A.F. Gharib ,
P.J. Holman  and
T.M. Craig
Kh.M. El-Dakhly*
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
A.S.M. Aboshinaf
Affiliation:
Provincial Laboratory of Animal Health Research Institute, Dokki, Giza, Egypt
W.M. Arafa
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
L.N. Mahrous
Affiliation:
Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
E. El-Nahass
Affiliation:
Department of Pathology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt
A.F. Gharib
Affiliation:
Provincial Laboratory of Animal Health Research Institute, Dokki, Giza, Egypt
P.J. Holman
Affiliation:
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University College Station, USA
T.M. Craig
Affiliation:
Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University College Station, USA
*
Author for Correspondence: Kh.M. El-Dakhly, Fax: +2 082 2327982; E-mail: eldakley_s71@yahoo.com
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Abstract

The effect of six available and commercial disinfectants on the embryonation and larval development of Toxascaris leonina eggs was studied. Dettol® and Virkon® both induced a 100% reduction in larval development (P ≤ 0.05). Dettol® resulted in deformed eggshells and a halt in embryonal development at 1 week post exposure. All Virkon®-treated eggs showed an early embryonic lysis 24 h post exposure. TH4+ and 70% ethanol both significantly (P ≤ 0.05) affected larval development, with 58.8 and 85.8% reduction, respectively. Neither sodium hypochlorite nor phenol significantly affected larval development (2.8 and 21.0%, respectively). Sodium hypochlorite treatment caused a visible decortication of the eggshell; however, phenol-treated embryonated Toxascaris eggs appeared more or less morphologically normal. In conclusion, the disinfectants tested induced variable degrees of decortication and suppression of larval development. Virkon®S was the most effective disinfectant against Toxascaris eggs, suggesting that it is the most advisable one to use. To the best of our knowledge, this is the first report of the use of Virkon®S as an ovicide and/or larvicide of helminths, particularly Toxascaris leonina.

Type
Research Paper
Information
Journal of Helminthology , Volume 92 , Issue 5 , September 2018 , pp. 530 - 534
Copyright
Copyright © Cambridge University Press 2017 

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References

Anderson, RC (2000) Nematode parasites of vertebrates, their development and transmission. 2nd edn. Wallingford, UK, CABI Publishing.Google Scholar
Aycicek, H, Yarsan, E, Sarimehmetoglu, HO, Tanyuksel, M, Girginkardesler, N and Ozyurt, M (2001) Efficacy of some disinfectants on embryonated eggs of Toxocara canis. Turkish Journal of Medical Sciences 31, 35–39.Google Scholar
Barriga, OO and Omar, HM (1992) Immunity to Toxocara vitulorum repeated infections in a rabbit model. Veterinary Immunopathology 33, 249–260.Google Scholar
Dalimi, A, Sattari, A and Motamedi, G (2006) A study on intestinal helminthes of dogs, foxes and jackals in the western part of Iran. Veterinary Parasitology 142, 129–133.Google Scholar
Dubna, S, Langrova, I, Napravnik, J, Jankovska, I, Vadlejch, J, Pekar, S and Fechtner, J (2007) The prevalence of intestinal parasites in dogs from Prague, rural areas, and shelters of the Czech Republic. Veterinary Parasitology 145, 120–128.Google Scholar
Dunn, AM (1978) Veterinary helminthology. 2nd edn. London, William Heinemann.Google Scholar
Epe, C (2009) Intestinal nematodes: biology and control. Veterinary Clinics of North America: Small Animal Practice 39, 1091–1107.Google Scholar
Feney-Rodriguez, S, Cuellar Del Hoyo, C and Guillenllera, JL (1988) Comparative study of the influence of light on embryonization of Toxocara canis, Toxascaris leonina and Ascaris suum. Revista Iberica de Parasitologia 48, 395–401.Google Scholar
Gasparini, R, Pozzi, T, Magnelli, R, Fatighenti, D, Giotti, E, Poliseno, G, Pratelli, M, Severini, R, Bonanni, P and De Feo, L (1995) Evaluation of in vitro efficacy of the disinfectant Virkon. European Journal of Epidemiology 11, 193–197.Google Scholar
Gibbons, LM, Jacobs, DE and Sani, RA (2001) Toxocara malaysiensis n. sp. (Nematoda: Ascaridoidea) from the domestic cat (Felis catus L.). Journal of Parasitology 87, 660–665.Google Scholar
Gonzalez, P, Carbonell, E, Urios, V and Rozhnov, VV (2007) Coprology of Panthera tigris altaica and Felis bengalensis euptilurus from the Russian Far East. Journal of Parasitology 93, 948–950.Google Scholar
Grooms, D (2003) Biosecurity guide for livestock farm visits. Michigan State University Extension Bulletin, E-2842.Google Scholar
Itoh, N, Kanai, K, Tominaga, H, Kawamata, J, Kaneshima, T, Chikazawa, S, Hori, Y, Hoshi, F and Higuchi, S (2011) Giardia and other intestinal parasites in dogs from veterinary clinics in Japan. Parasitology Research 109, 253–256.Google Scholar
Kim, Y-H and Huh, S (2005) Prevalence of Toxocara canis, Toxascaris leonina and Dirofilaria immitis in dogs in Chuncheon, Korea (2004). Korean Journal of Parasitology 43, 65–67.Google Scholar
Koski, P, Anttila, P and Kuusela, J (2016) Killing of Gyrodactylus salaris by heat and chemical disinfection. Acta Veterinaria Scandinavica 58, 21. http://doi.org/10.1186/s13028-016-0202-y.Google Scholar
Labarthe, N, Serrao, ML, Ferreira, AMR, Almeida, NKO and Guerrero, J (2004) A survey of gastrointestinal helminths in cats of the metropolitan region of Rio de Janeiro, Brazil. Veterinary Parasitology 123, 133–139.Google Scholar
McDonnell, G and Russell, AD (1999) Antiseptics and disinfectants: activity, action, and resistance. Clinical Microbiology Reviews 12, 147–179.Google Scholar
Møretrø, T, Vestby, LK, Nesse, LL, Storheim, SE, Kotlarz, K and Langsrud, S (2009) Evaluation of efficacy of disinfectants against Salmonella from the feed industry. Journal of Applied Microbiology 106, 1005–1012.Google Scholar
Morrondo, P, Díez-Morrondo, C, Pedreira, J, Díez-Baños, N, Sánchez-Andrade, R, Paz-Silva, A and Díez-Baños, P (2006) Toxocara canis larvae viability after disinfectant – exposition. Parasitology Research 99, 558–561.Google Scholar
Morton, HE (1983) Alcohols. pp. 225–239 in Bloch, SS (Ed.) Disinfection, sterilization, and preservation. 3rd edn. Philadelphia, Lea & Febiger.Google Scholar
Oh, K-S, Kim, G-T, Ahn, K-S and Shin, S-S (2016) Effects of disinfectants on larval development of Ascaris suum eggs. Korean Journal of Parasitology 54, 103–107.Google Scholar
Okoshi, S and Usui, M (1968) Experimental studies on Toxascaris leonina. IV. Development of eggs of three ascarids, T. leonina, Toxocara canis and Toxocara cati, in dogs and cats. Japanese Journal of Veterinary Science 30, 29–38.Google Scholar
Okulewicz, A, Perec-Matysiak, A, Buńkowska, K and Hildebrand, J (2012) Toxocara canis, Toxocara cati and Toxascaris leonina in wild and domestic carnivores. Helminthologia 49, 3–10.Google Scholar
Parsons, JC (1987) Ascarid infections of cats and dogs. Veterinary Clinics of North America: Small Animal Practice 17, 1307–1339.Google Scholar
Pawar, RM, Lakshimkantan, U, Hasan, S, Poornachandar, A and Shivaji, S (2012) Detection and molecular characterization of ascarid nematode infection (Toxascaris leonina and Toxocara cati) in captive Asiatic lions (Panthera leo persica). Acta Parasitologica 57, 67–73.Google Scholar
Prokopic, J and Figallova, V (1982) The migration of larvae of Toxascaris leonina (Linstow, 1909) in experimentally infected white mice. Folia Parasitologica (Praha) 29, 233–238.Google Scholar
Reperant, LA, Hegglin, D, Fischer, C, Kohler, L, Weber, M and Deplazes, P (2007) Influence of urbanization on the epidemiology of intestinal helminths of the red fox (Vulpes vulpes) in Geneva, Switzerland. Parasitology Research 101, 605–611.Google Scholar
Russell, AD and Furr, JR (1977) The antibacterial activity of a new chloroxylenol formulation containing ethylenediaminetetraacetic acid. Journal of Applied Bacteriology 43, 253–260.Google Scholar
Sabry, MA (1999) Epidemiological studies on toxocariasis in animals and man. PhD thesis (Zoonoses), Faculty of Veterinary Medicine, Cairo University.Google Scholar
Scott, TA and Swetnam, C (1993) Screening sanitizing agents and methods of application for hatching eggs, 2. Effectiveness against microorganisms on the egg shell. Journal of Applied Poultry Research 2, 7–11.Google Scholar
Sharma, S (1997) Approaches to design and synthesis of antiparasitic drugs. Vol. 25. Amsterdam, Elsevier.Google Scholar
Sommerfelt, IE, Cardillo, N, Lopez, C, Ribicich, M, Gallo, C and Franco, A (2006) Prevalence of Toxocara cati and other parasites in cats’ faeces collected from the open spaces of public institutions: Buenos Aires, Argentina. Veterinary Parasitology 140, 296–301.Google Scholar
Soulsby, EJL (1982) Helminthes, arthropods and Protozoa of domesticated animals. 7th edn. London, Bailliere, Tindall and Cassel.Google Scholar
Sprent, JFA (1959) The life history and development of Toxascaris leonina (von Linstow 1902) in the dog and cat. Parasitology 49, 330–371.Google Scholar
Tarsitano, E, Greco, G, Decaro, N, Nicassio, F, Lucente, MS, Buonavogliam, C and Tempesta, M (2010) Environmental monitoring and analysis of faecal contamination in an urban setting in the city of Bari (Apulia Region, Italy): health and hygiene implications. International Journal of Environmental Research and Public Health 7, 3972–3986.Google Scholar
Traversa, D (2012) Pet roundworms and hookworms: A continuing need for global worming. Parasites & Vectors 5, 91. doi:10.1186/1756-3305-5-91.Google Scholar
Turpin, K (2013) Ethanol vs. isopropyl alcohol to disinfect. Available at http://www.ehow.com/about_6540795_ethanol-vs_-isopropyl-alcoholdisinfect.html (accessed 16 September 2017).Google Scholar
Urquhart, GM, Armour, J, Duncan, JL, Dunn, AM and Jennings, FW (1996) Veterinary parasitology. 2nd edn. Oxford, UK, Blackwell Science.Google Scholar
Verocai, G, Tavares, P, Ribeiro, DA, Correia, T and Scott, F (2010) Effects of disinfectants on Toxocara canis embryogenesis and larval establishment in mice tissues. Zoonoses and Public Health 57, e213–e216.Google Scholar
Wright, I, Stafford, K and Coles, G (2016) The prevalence of intestinal nematodes in cats and dogs from Lancashire, north-west England. Journal of Small Animal Practice 57, 393–395.Google Scholar
Wright, WH (1935) Observations on the life history of Toxascaris leonina (Nematoda: Ascaridae). Proceedings of the Helminthological Society of Washington 2, 56.Google Scholar
Yasuda-Yasuki, Y, Namiki-Kanie, S and Hachisaka, Y (1978) Inhibition of germination of Bacillus subtilis spores by alcohols, pp. 113–116 in Chambliss, G and Vary, JC (Eds) Spores VII. Washington, DC, American Society for Microbiology.Google Scholar
Zeweil, HS, Rizk, RE, Bekhet, GM and Ahmed, MR (2015) Comparing the effectiveness of egg disinfectants against bacteria and mitotic indices of developing chick embryos. Journal of Basic and Applied Zoology 70, 1–15.Google Scholar