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Morphological and DNA Barcoding Based Identification of Calotropis procera (Aiton) Dryand. and Calotropis gigantea (L.) Dryand.

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Abstract

The precise identification of plant species is exclusively important in forensic studies and plants of medicinal interest. In addition to conventional methods, DNA barcoding is coming up as a supportive tool in the identification of biological entities, including plant species. DNA barcoding may be useful, especially when plant species are indistinguishable during their vegetative phase of growth. The present study focused on the identification of Calotropis procera and C. gigantea through morphological characters and DNA barcoding analysis. The markers (rbcL, matK, and ITS) used for DNA barcoding could not differentiate these two species, whereas morphological parameters are distinct enough to establish their identity. The BLAST analysis has shown 100% similarity for C. procera and C. gigantea with rbcL, matK, and ITS markers.

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ACKNOWLEDGMENTS

Authors are thankful to the Chairperson, Department of Botany and the Chairperson, Institute of Forensic Science and Criminology. Panjab University, Chandigarh for providing necessary facilities during this work.

Funding

DBT grant BT/IN/Indo-US/Foldscpe/39/2015 and DST purse from Govt of India are thankfully acknowledged.

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Authors and Affiliations

  1. Department of Botany, Panjab University, 160014, Chandigarh, India

    M. C. Sidhu & A. Kumari

  2. Institute of Forensic Science and Criminology, Panjab University, 160014, Chandigarh, India

    B. Jain, S. Kaur, A. Kamra & J. Rai

Authors
  1. M. C. Sidhu
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  2. A. Kumari
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  3. B. Jain
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  4. S. Kaur
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  5. A. Kamra
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  6. J. Rai
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Correspondence to J. Rai.

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The authors declare that they have no conflicts of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Sidhu, M.C., Kumari, A., Jain, B. et al. Morphological and DNA Barcoding Based Identification of Calotropis procera (Aiton) Dryand. and Calotropis gigantea (L.) Dryand.. Biol Bull Russ Acad Sci 50, 474–477 (2023). https://doi.org/10.1134/S1062359022602968

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  • DOI: https://doi.org/10.1134/S1062359022602968

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