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In vitro micropropagation and genetic fidelity assesment in Flemingia macrophylla (Willd.) Merr: an ethnomedicinal plant

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Abstract

Flemingia macrophylla is an important ethnomedicinal plant of Fabaceae, with immense pharmaceutical properties. Now, it is becoming rare because of its poor seed dispersal mechanism and over-exploitation of the material. In this study we have successfully established an in vitro micro-propagation protocol employing explants like ex vivo leaf and cotyledonary node. Both the explants were cultured on MS medium supplemented with various combinations and concentrations of growth hormones. In vitro morphogenetic response of leaf explants are very poor when compared with cotyledonary nodes. Cotyledonary nodes cultured on MS medium supplemented with BAP (2.0 mg/l) in combination with IAA (0.2 mg/l) produced a high percentage of multiple shoots (13.76 ± 1.1). Elongated shoots (6.30 ± 0.01 cm) rooted on MS medium with IBA (0.8 mg/l) produced a high frequency of rooting (10.80 ± 1.23) with excellent root length (10.56 ± 1.70 cm). Plantlets with a fully developed root system were acclimatized using soil: manure (1:1) and shifted to the greenhouse. Investigations for the corroboration of genetic stability among the clones and mother plants were carried out by using ISSR and RAPD markers and the results were in clear evidence that all the plants are true-to-type. This scientific approach for in vitro propagation and conservation is prerequisite for better sustainability of the plant.

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Abbreviations

BAP:

6-benzylaminopurine

KN:

Kinetin

IBA:

Indole-3-butyricacid

ISSR:

Inter simple-sequence repeats

RAPD:

Random amplified polymorphic DNA

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Acknowledgements

Authors are greatly acknowledged to Prof. A. Sadanandam CSIR-Emeritus Scientist, Department of Biotechnology, Kakatiya University for his kind advice and encouragement. We are greatly acknowledged to Prof. V. S. Raju Department of Botany, Kakatiya University for authentication of plant and encouragement. Authors are also greatful to UGC, New Delhi for sanctioning SAP DRS programme to the department.

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Authors and Affiliations

  1. Department of Biotechnology, Kakatiya University, Warangal, 506009, India

    Abhiteja Sirikonda, Phanikanth Jogam, Raghu Ellendula, Hemalatha Kudikala, Kasim Mood & Venkateswar Rao Allini

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  1. Abhiteja Sirikonda
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  2. Phanikanth Jogam
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  3. Raghu Ellendula
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  4. Hemalatha Kudikala
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  6. Venkateswar Rao Allini
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Correspondence to Venkateswar Rao Allini.

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Sirikonda, A., Jogam, P., Ellendula, R. et al. In vitro micropropagation and genetic fidelity assesment in Flemingia macrophylla (Willd.) Merr: an ethnomedicinal plant. Vegetos 33, 286–295 (2020). https://doi.org/10.1007/s42535-020-00106-9

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