Abstract
Three new species of Hypoxylon (Xylariaceae) collected from Martinique in the French Caribbean are recognised by new combinations of morphological characters. Their status as undescribed taxa was supported by secondary metabolite profiling based on High performance liquid chromatography with diode array and mass spectrometric detection (HPLC/DAD-MS) as well as by comparison of ITS and partial ß-tubulin DNA sequences with related taxa. In the course of this study, the teleomorph of Nodulisporium griseobrunneum was found, and this species could be transferred to Hypoxylon. Moreover, several names in Hypoxylon are epitypified by selecting recently collected specimens from the same geographic areas as the holotypes came from. Despite the fact that our study used the hitherto most extensive taxon sampling, the phylogenetic analyses inferred from ITS and ß-tubulin sequences remain contradictory to each other, and neither genealogy was found fully in agreement with phenotype-derived traits. We conclude that the right gene (or multi-gene genealogies) to reflect the phylogeny and evolution of Hypoxylon still remains to be found. For the time being, we recommend that the application of polyphasic taxonomic concepts should be continued in taxonomic studies of Hypoxylon.
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Acknowledgments
We gratefully acknowledge support from the curators of various international culture collections and herbaria, above all BPI, CBS, GAM, K, LIP, MUCL, NY and WSP, who provided important specimens for the present study. We also thank Markus Scholler (KR) for mediating international loan transactions, and Régis Courtecuisse for facilitating field work in the French Caribbean. Christian Lechat and Thomas Læssøe are thanked for their continuous support in finding important specimens during the field trips in the Neotropics and elsewhere, and all other co-authors of the cited publications by D. P., J. F., and M.S. are thanked for their help. The field sampling in French Guiana has also benefited from “Investissements d’Avenir” grants of the ANR (CEBA ANR-10-LABX-0025, CNRS Cayenne and Toulouse) to J.F. and co-workers. Gerhard Rambold (Univ. Bayreuth) is gratefully acknowledged for his support of our studies during supervision of the MSc thesis of E. K. Last but not least, Beata Schmieschek, Christina Leistner and Philine Wotsch are thanked for expert technical assistance, and the excellent performance of Simone Heitkämper during her student project is gratefully acknowledged.
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Taxonomic Novelties: Hypoxylon griseobrunneum (B.S. Mehrotra) J. Fourn., Kuhnert & M. Stadler, comb. nov.; Hypoxylon isabellinum J. Fourn., Kuhnert & M. Stadler sp. nov.; Hypoxylon laminosum J. Fourn., Kuhnert & M. Stadler, sp. nov.; Hypoxylon lateripigmentum J. Fourn., Kuhnert & M. Stadler, sp. nov.
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Kuhnert, E., Fournier, J., Peršoh, D. et al. New Hypoxylon species from Martinique and new evidence on the molecular phylogeny of Hypoxylon based on ITS rDNA and β-tubulin data. Fungal Diversity 64, 181–203 (2014). https://doi.org/10.1007/s13225-013-0264-3
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DOI: https://doi.org/10.1007/s13225-013-0264-3