Abstract
Taro bacilliform CH virus (TaBCHV) is the second badnavirus found infecting taro (Colocasia esculenta), after Taro bacilliform virus (TaBV). We determined the complete genome of TaBCHV Hawaii Strain (KY359389) from taro in Hawaii and examined its occurrence and distribution. Alignment analysis showed the TaBCHV Hawaii Strain has 93% and 94% sequence identity to China strains TaBCHV-1 and TaBCHV-2 respectively. Phylogenetic analysis basing on the whole genome sequence of 21 selected badnaviruses, showed that the TaBCHV Hawaii Strain clustered within the same branch as the two China strains. All sequences from available TaBCHV strains are most closely related to Piper yellow mottle virus (PYMoV; NC022365) and are most distant from TaBV (NC004450). We used RT-PCR to determine the occurrence and distribution of TaBCHV on taro in Hawaii. The positive rates of the virus on the five islands were 72% on Oahu, 53% on Hawaii, 89% on Maui, 73% on Molokai, and 78% on Kauai, with a mean positive rate of 73%. This is the first report of the complete genome sequence and the first report of the occurrence of this virus in the United States.
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Change history
29 November 2017
Due to some error captions for Fig. 1 and Fig. 2 have somehow been switched and needs to be read as: Fig. 1: Genome organization of Taro bacilliform CH virus (TaBCHV) Hawaii Srain.
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Acknowledgements
We thank Dr. Fred Brooks for his critical reviews of this manuscript. The research was supported in part by the USDA National Institute of Food and Agriculture, Hatch HAW09025-H (1001478), and the USDA Agricultural Research Service (58-5320-4-012). Financial support was also provided by research funds from China, National Nature Science Foundation (31201487), China Agriculture Research System (Cars-28), Hebei Natural Science Foundation of Higher Education Institutions for Youth (YQ2014023) and the support plan for youth talent in Hebei province.
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The authors declare that they have no conflicts of interest. This research did not involve human participants or any animal experimentation.
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A correction to this article is available online at https://doi.org/10.1007/s10658-017-1391-6.
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Wang, Y., Borth, W.B., Green, J.C. et al. Genome characterization and distribution of Taro bacilliform CH virus on taro in Hawaii, USA. Eur J Plant Pathol 150, 1107–1111 (2018). https://doi.org/10.1007/s10658-017-1353-z
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DOI: https://doi.org/10.1007/s10658-017-1353-z