Abstract
The badnavirus taro bacilliform virus (TaBV) has been reported to infect taro (Colocasia esculenta L.) and other edible aroids in several South Pacific island countries, but there are no published reports from Australia. Using PCR and RCA, we identified and characterized an Australian TaBV isolate. A terminally redundant cloned copy of the TaBV genome was generated and shown to be infectious in taro following agro-inoculation. This is the first report of TaBV from Australia and also the first report of an infectious clone for this virus.
References
Bomer M, Turaki A, Silva G, Kumar P, Seal S (2016) A sequence-independent strategy for amplification and characterization of episomal badnavirus sequences reveals three previously uncharacterized yam badnaviruses. Viruses 8:188
Boulton M, Buchholz W, Marks M, Markham P, Davies J (1989) Specificity of Agrobacterium-mediated delivery of maize streak virus DNA to members of the Gramineae. Plant Mol Biol 12:31–40
Bouhida M, Lockhart B, Olszewski N (1993) An analysis of the complete sequence of a sugarcane bacilliform virus genome infectious to banana and rice. J Gen Virol 74:15–22
Geering A, Hull R (2012) Caulimoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, ninth report of the International Committee on Taxonomy of Viruses. Elsevier, Amsterdam, pp 429–443
Gollifer D, Jackson G, Dabek A, Plumb R, May Y (1977) The occurrence and transmission of viruses of edible aroids in the Solomon Islands and the Southwest Pacific. Int J Pest Manag 23:171–177
Haible D, Kober S, Jeske H (2006) Rolling circle amplification revolutionizes diagnosis and genomics of geminiviruses. J Virol Methods 135:9–16
Higgins C, Bejerman N, Li M, James A, Dietzgen R, Pearson M, Revill P, Harding R (2016) Complete genome sequence of Colocasia bobone disease-associated virus, a putative cytorhabdovirus infecting taro. Arch Virol 161:745–748
Huang Q, Hartung J (2001) Cloning and sequence analysis of an infectious clone of citrus yellow mosaic virus that can infect sweet orange via Agrobacterium-mediated inoculation. J Gen Virol 82:2549–2558
Jacquot E, Hagen L, Jacquemond M, Yot P (1996) The open reading frame 2 product of cacao swollen shoot badnavirus is a nucleic acid-binding protein. Virology 225:191–195
Jacquot E, Hagen L, Michler P, Rohfritsch O, Stussi-Garaud C, Keller M, Jacquemond M, Yot P (1999) In situ localization of cacao swollen shoot virus in agroinfected Theobroma cacao. Arch Virol 144:259–271
James A, Geijskes R, Dale J, Harding R (2011) Development of a novel rolling-circle amplification technique to detect Banana streak virus that also discriminates between integrated and episomal virus sequences. Plant Dis 95:57–62
James M, Kenten R, Woods R (1973) Virus-like particles associated with two diseases of Colocasia esculenta (L.) Schott in the Solomon Islands. J Gen Virol 21:145–153
Kazmi SA, Yang Z, Hong N, Wang G, Wang Y (2015) Characterization by small RNA sequencing of taro bacilliform CH virus (TaBCHV), a novel badnavirus. PLoS One 10:e0134147
Macanawai A, Ebenebe A, Hunter D, Devitt L, Hafner G, Harding R (2005) Investigations into the seed and mealybug transmission of taro bacilliform virus. Aust Plant Pathol 34:73–76
Medberry S, Lockhart B, Olszewski N (1990) Properties of Commelina yellow mottle virus’s complete DNA sequence, genomic discontinuities and transcript suggest that it is a pararetrovirus. Nucleic Acids Res 18:5505–5513
Ming SFY, Ping GW, Ping LW, Xing WX, Ni H (2013) Molecular identification and specific detection of badnavirus from taro grown in China. Acta Phytopathol Sinica 6:590–595
Revill P, Jackson G, Hafner G, Yang I, Maino M, Dowling M, Devitt L, Dale J, Harding R (2005) Incidence and distribution of viruses of taro (Colocasia esculenta) in pacific island countries. Aust Plant Pathol 35:327–331
Sainsbury F, Thuenemann C, Lomonossoff P (2009) pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants. Plant Biotechnol J 7:682–693
Sukal A, Kidanemariam D, Dale J, James A, Harding R (2017) Characterization of badnaviruses infecting Dioscorea spp. in the pacific reveals two putative novel species and the first report of Dioscorea bacilliform RT virus 2. Virus Res 238:29–34
Wang Y, Hu J, Borth WB, Hamim I, Green JO, Melzer M (2017) First report of taro bacilliform CH virus (TaBCHV) on taro (Colocasia esculenta) in Hawaii, USA. Plant Dis 101:1334
Wu C, Lai Y, Lin N, Hsu Y, Tsai H, Liao J, Hu C (2008) A simplified method of constructing infectious clones of begomovirus employing limited restriction enzyme digestion of products of rolling circle amplification. J Virol Methods 147:355–359
Yang I, Hafner G, Dale J, Harding R (2003) Genomic characterization of taro bacilliform virus. Arch Virol 148:937–949
Yang I, Hafner G, Revill P, Dale J, Harding R (2003) Sequence diversity of south pacific isolates of taro bacilliform virus and the development of a PCR-based diagnostic test. Arch Virol 148:1957–1968
Acknowledgements
The authors are grateful to Dr. Ben Dugdale, Queensland University of Technology, for providing the pOPT-NXT vector for cloning purposes. DK is the recipient of an Australia Awards Scholarship.
Data availability
Sequences described in this paper are available under GenBank accession numbers MG017318-MG017320.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare they have no conflict of interest.
Ethical approval
This article does not contain any work conducted on animals or human participants.
Additional information
Handling Editor: Sead Sabanadzovic.
Rights and permissions
About this article
Cite this article
Kidanemariam, D.B., Sukal, A.C., Crew, K. et al. Characterization of an Australian isolate of taro bacilliform virus and development of an infectious clone. Arch Virol 163, 1677–1681 (2018). https://doi.org/10.1007/s00705-018-3783-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-018-3783-0