Abstract
Verrucaria pakistanica is described as a novel species from District Kohistan, Khyber Pakhtunkhwa Province. It is characterised by thick, deeply areolate thallus, large superficial perithecia, a thick involucrellum (80–105 µm), i.e., conical, not reaching the substratum and larger ascospores (20–29 × 7–10 µm) than the similar V. muralis. Phylogeny of ITS and nuLSU region confirms its position within the genus Verrucaria and morphological comparison makes it distinct from other related species of the genus.
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Acknowledgements
The financial assistance provided by University of the Punjab, Quaid–e–Azam Campus, Lahore, under PU Research Project for the fiscal year 2021–2022 is gratefully acknowledged. We are also thankful to Dr. Jan Vondrak, Department of Botany, Faculty of Science, University of South Bohemia, CZ–370 05 České Budějovice, Czech Republic, for his pre submission review of the manuscript.
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The funding for this research work provided by University of the Punjab, Quaid–e–Azam Campus, Lahore, under PU Research Project for the fiscal year 2021–2022.
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Supplementary Information
Below is the link to the electronic supplementary material.
Online Resource 1
A brief comparison of Verrucaria pakistanica with other morphologically and phylogenetically related taxa (FAS 15 KB)
Online Resource 2
Sequences used in the phylogenetic analyses. Voucher numbers their country of origin are given. Sequences generated from local collection are marked as bold (FAS 8 KB)
Online Resource 3
The online version contains alignment used to produce phylogeny of nrITS dataset (PDF 392 KB)
Online Resource 4
The online version contains alignment used to produce phylogeny of nuLSU dataset (PDF 586 KB)
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Information on Electronic Supplementary Material
Online Resource 1. A brief comparison of Verrucaria pakistanica with other morphologically and phylogenetically related taxa.
Online Resource 2. Sequences used in the phylogenetic analyses. Voucher numbers their country of origin are given. Sequences generated from local collection are marked as bold.
Online Resource 3. The online version contains alignment used to produce phylogeny of nrITS dataset.
Online Resource 4. The online version contains alignment used to produce phylogeny of nuLSU dataset.
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Khalid, A.N., Zulfiqar, R. A new species of the genus Verrucaria (lichenized Ascomycetes: Verrucariaceae) from Kohistan district, Pakistan. Plant Syst Evol 309, 18 (2023). https://doi.org/10.1007/s00606-023-01853-3
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DOI: https://doi.org/10.1007/s00606-023-01853-3