Figure 1.
Phylogenetic analyses of Sarcophyton species and relationship with chemotypes.
Phylogentetic tree of an alignment of utilizing mitochondrial protein-coding genes MutS homolog msh1 sequences for Sarcophyton specimens constructed by the maximum likelihood (ML) method. Values at branches represent ML, neighbor-joining (NJ) and maximum parsimony (MP) method bootstrap values, respectively. Monophylies with more than 95% Bayesian posterior probabilities are shown by thick branches. Sequences in bold without GenBank accession numbers are msh1 sequences newly obtained in this study. Color dots indicate different chemotypes as described in this study. For chemotype information see Figure 2 and for specimen information see Table 1.
Figure 2.
Structures of cembranes from Sarcophyton species identified in this study.
Colored dots next to each cembrane are the same as in other figures.
Table 1.
Summary of field sites and chemotypes.
Figure 3.
In situ photographs of colonies of Sarcophyton.
A. Sarcophyton trocheliophorm, Sunabe 12. B. Sarcophyton glaucum clade B, Sunabe 13. C. Sarcophyton glaucum clade C, Mizugama 7. D. Sarcophyton glaucum clade D, Sunabe 17. E. Sarcophyton glaucum clade F, Zanpa 3. F. Sarcophyton ehrenbergi mixed clade, Sunabe 1.
Figure 4.
Sclerites of Sarcophyton glaucum clades B, D and F, and their averages length and width.
All sclerites shown are surface sclerites; Clade B obtained from specimens Sunabe 6 and Sunabe 13; Clade D from Sunabe 2, Sunabe 19; Clade F from Zanpa 3, Mizugama 5, Mizugama 9. Images were taken using a scanning electron microscope.
Table 2.
Mean and standard deviation (SD) of length and width (mm) from sclerites of each phylogenetic clade.
Figure 5.
Scatter plot and regression line of length and width of sclerites of each Sarcophyton glaucum clade.
Horizontal axis: width of sclerites, vertical axis: length of sclerites.
Figure 6.
Map of collection sites of specimens examined in this study.
Okinawa Island is located in southern Japan.